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1.
Oncol Rep ; 41(1): 711-717, 2019 Jan.
Article in English | MEDLINE | ID: mdl-30542721

ABSTRACT

In attempting to identify effective anticancer drugs from natural products that are harmless to humans, we found that the gomisin J from Schisandra chinensis fruit has anticancer activity. Schisandra chinensis fruits are used in traditional herbal medicine and gomisin J is one of their chemical constituents. In the present study, we examined the anticancer activity of gomisin J in MCF7 and MDA-MB-231 breast cancer cell lines and in MCF10A normal cell line, in a time- and concentration-dependent manner. Our data revealed that gomisin J exerted a much stronger cytotoxic effect on MCF7 and MDA-MB-231 cancer cells than on MCF10A normal cells. Gomisin J suppressed the proliferation and decreased the viability of MCF7 and MDA-MB-231 cells at relatively low (<10 µg/ml) and high (>30 µg/ml) concentrations, respectively. Our data also revealed that gomisin J induced necroptosis, a programmed form of necrosis, as well as apoptosis. Notably, gomisin J predominantly induced necroptosis in MCF7 cells that are known to have high resistance to many pro-apoptotic anticancer drugs, while MDA-MB-231 exhibited a much lower level of necroptosis but instead a higher level of apoptosis. This data indicated the possibility that it may be used as a more effective anticancer drug, especially in apoptosis-resistant malignant cancer cells. In an extended study, gomisin J exhibited a strong cytotoxic effect on all tested various types of 13 cancer cell lines, indicating its potential to be used against a wide range of different types of cancer cells.


Subject(s)
Lignans/pharmacology , Neoplasms/drug therapy , Polycyclic Compounds/pharmacology , Schisandra/chemistry , Apoptosis/drug effects , Cell Line, Tumor , Cell Proliferation/drug effects , Drug Screening Assays, Antitumor , Fruit/chemistry , Humans , Lignans/therapeutic use , Polycyclic Compounds/therapeutic use
2.
Nat Prod Commun ; 12(1): 21-22, 2017 Jan.
Article in English | MEDLINE | ID: mdl-30549816

ABSTRACT

MeOH. extracts of Kaenpferia parvflora Wall.- ex. Baker family Zingiberaceae; were consecutively partitioned- with CHCl3, EtOAc, nd n-BuOH. The CHC1, fractions were diluted in distilled water with n-hexane-CH2Cl2 and three methoxyflavones were isolated from. the CH2Cl2 extract. Based on-spectral analysis and comparison of the spectral data with literature values, the compounds. were identified as; 3,5,7,3',4'-pentamethoxyflavone (KPl), 5,7- dimethoxyflavone (KP2), and 5,7,4'-trimethoxyflavone (KP3). In relation to their possible effectiveness against Alzheimher's disease;these-compounds were tested for their ability to inhibit acetylcholinesterase activity and neurite outgrowth in the PC12 cell line. Of the three compounds, KPl was the only one to inhibit significantly the acetylcholinesterase activity in a dose-dependent manner.


Subject(s)
Cholinesterase Inhibitors/pharmacology , Flavones/pharmacology , Zingiberaceae/chemistry , Alzheimer Disease/drug therapy , Animals , Dose-Response Relationship, Drug , Humans , Neurites/drug effects , PC12 Cells , Plant Extracts/chemistry , Plant Extracts/pharmacology , Rats , Rhizome/chemistry , Solvents
3.
Nat Prod Commun ; 9(12): 1723-4, 2014 Dec.
Article in English | MEDLINE | ID: mdl-25632468

ABSTRACT

The present study evaluated the antidiabetic effect of osajin and pomiferin from the osajin orange in normal and streptozotocin-induced diabetic rats. Pomiferin in the streptozotocin-induced diabetic effects showed significant hypoglycemic activity for 14 days significantly decreased the serum glucose, triglyceride while it increased the serum insulin in diabetic rats but not in normal rats (p < 0.05; at doses of 100 and 300 mg/kg for 14 days). Pomiferin showed potential in anti-diabetic effects compared to osajin. It also has no effects on C-peptide (ECLIA). Further structure-activity relationships of aromatic position 3 on ring B from osajin and pomiferin will be reported in due course.


Subject(s)
Benzopyrans/therapeutic use , Diabetes Mellitus, Experimental/drug therapy , Hypoglycemic Agents/therapeutic use , Isoflavones/therapeutic use , Animals , Male , Rats , Streptozocin
4.
Nat Prod Commun ; 9(9): 1295-8, 2014 Sep.
Article in English | MEDLINE | ID: mdl-25918796

ABSTRACT

Radiotherapy is an important form of treatment for a wide range of cancers, but it can damage DNA and cause adverse effects. We investigated if the diplacone analogs of P. tomentosa were radio-protective in a human lymphoblastoid cell line (AHH-1). Four geranylated flavonoids, diplacone, 3'-O-methyl-5'-hydroxydiplacone, 3'-O-methyl-5'-O-methyldiplacone and 3'-O-methyldiplacol, were tested for their antioxidant and radio-protective effects. Diplacone analogs effectively scavenged free radicals and inhibited radiation-induced DNA strand breaks in vitro. They significantly decreased levels of reactive oxygen species and cellular DNA damage in 2 Gy-irradiated AHH-1 cells. Glutathione levels and superoxide dismutase activity in irradiated AHH-1 cells increased significantly after treatment with these analogs. The enhanced biological anti-oxidant activity and radioprotective activity of diplacone analogs maintained the survival of irradiated AHH-1 cells in a clonogenic assay. These data suggest that diplacone analogs may protect healthy tissue surrounding tumor cells during radiotherapy to ensure better control of radiotherapy and allow higher doses of radiotherapy to be employed.


Subject(s)
DNA Damage/drug effects , DNA Damage/radiation effects , Flavonoids/pharmacology , Magnoliopsida/chemistry , Plant Extracts/pharmacology , Radiation-Protective Agents/pharmacology , Antioxidants/chemistry , Antioxidants/pharmacology , Cell Line, Tumor , Flavonoids/chemistry , Gamma Rays/adverse effects , Humans , Neoplasms/drug therapy , Neoplasms/metabolism , Neoplasms/radiotherapy , Oxidation-Reduction , Plant Extracts/chemistry , Radiation-Protective Agents/chemistry , Reactive Oxygen Species/metabolism
5.
Int J Oncol ; 43(6): 1943-50, 2013 Dec.
Article in English | MEDLINE | ID: mdl-24141596

ABSTRACT

The biochemical mechanisms of cell death by oleifolioside B (OB), a cycloartane-type triterpene glycoside isolated from Dendropanax morbifera Leveille, were investigated in A549 human lung carcinoma cells. Our data indicated that exposure to OB led to caspase activation and typical features of apoptosis; however, apoptotic cell death was not prevented by z-VAD-fmk, a pan-caspase inhibitor, demonstrating that OB-induced apoptosis was independent of caspase activation. Subsequently, we found that OB increased autophagy, as indicated by an increase in monodansylcadaverine fluorescent dye-labeled autophagosome formation and in the levels of the autophagic form of microtubule-associated protein 1 light chain 3 and Atg3, an autophagy-specific gene, which is associated with inhibiting phospho-nuclear factor erythroid 2-related factor 2 (Nrf2) expression. However, pretreatment with bafilomycin A1, an autophagy inhibitor, attenuated OB-induced apoptosis and dephosphorylation of Nrf2. The data suggest that OB-induced autophagy functions as a death mechanism in A549 cells and OB has potential as a novel anticancer agent capable of targeting apoptotic and autophagic cell death and the Nrf2 signaling pathway.


Subject(s)
Apoptosis/drug effects , Autophagy/drug effects , Carcinoma, Non-Small-Cell Lung/drug therapy , Lung Neoplasms/drug therapy , Saponins/pharmacology , Amino Acid Chloromethyl Ketones/pharmacology , Antineoplastic Agents/pharmacology , Autophagy-Related Proteins , CASP8 and FADD-Like Apoptosis Regulating Protein/biosynthesis , Caspase 3/metabolism , Caspase 8/metabolism , Caspase 9/metabolism , Caspase Inhibitors/pharmacology , Cell Line, Tumor , Enzyme Inhibitors/pharmacology , Humans , Inhibitor of Apoptosis Proteins/biosynthesis , Macrolides/pharmacology , Microtubule-Associated Proteins/biosynthesis , Microtubule-Associated Proteins/metabolism , NF-E2-Related Factor 2/biosynthesis , NF-E2-Related Factor 2/metabolism , Plant Extracts/pharmacology , Proto-Oncogene Proteins c-bcl-2/biosynthesis , Survivin , Ubiquitin-Conjugating Enzymes/biosynthesis , Ubiquitin-Conjugating Enzymes/metabolism
6.
Indian J Biochem Biophys ; 50(6): 485-91, 2013 Dec.
Article in English | MEDLINE | ID: mdl-24772972

ABSTRACT

Triptolide, a diterpene derived from Tripterygium wilfordii Hook f., a Chinese medicinal herb, has been reported to inhibit cell proliferation and induce apoptosis in various human cancer cells, but its anticancer effects on human osteosarcoma cells have not yet been elucidated. In this study, we investigated whether triptolide induces apoptosis in human osteosarcoma cells and the underlying molecular mechanisms. We firstly demonstrated that triptolide inhibited cell growth and induced apoptosis in U2OS cells. Western blot analysis showed that the levels of procaspase-8, -9, Bcl-2, Bid and mitochondrial cytochrome c were downregulated in triptolide-treated U2OS cells, whereas the levels of Fas, FasL, Bax, cytosolic cytochrome c, cleaved caspase-3 and cleaved PARP were upregulated. These results suggest that triptolide induces apoptosis in U2OS cells by activating both death receptor and mitochondrial apoptotic pathways.


Subject(s)
Antineoplastic Agents/pharmacology , Apoptosis/drug effects , Diterpenes/pharmacology , Osteosarcoma/pathology , Phenanthrenes/pharmacology , Caspase 3/metabolism , Cell Line, Tumor , Cell Proliferation/drug effects , Enzyme Activation/drug effects , Epoxy Compounds/pharmacology , G2 Phase Cell Cycle Checkpoints/drug effects , Humans , M Phase Cell Cycle Checkpoints/drug effects , Mitochondria/drug effects , Mitochondria/metabolism , Poly(ADP-ribose) Polymerases/metabolism , Proteolysis/drug effects
7.
Article in English | MEDLINE | ID: mdl-25945102

ABSTRACT

Triptolide (TPL) has been shown to inhibit cell proliferation and induce apoptosis in various human cancer cells; however, the precise mechanism of apoptosis induced by TPL in human melanoma cells has not yet been elucidated. In this study, we investigated the precise mechanism underlying cytocidal effects of TPL on human melanoma cells. Treatment of human melanoma cells with TPL significantly inhibited cell growth and induced apoptosis, as evidenced by flow cytometry and annexin V-fluorescein isothiocyanate analyses. TPL increased the levels of Fas and Fas-associated death domain (FADD) and induced cleavage of Bid by activation of caspase-8 and cytochrome c release from mitochondria to the cytosol, which resulted in activation of caspase-9 and caspase-3. Moreover, TPL-induced apoptosis in SK-MEL-2 cells was mediated through dephosphorylation of focal adhesion kinase (FAK) and its cleavage by caspase-8-mediated caspase-3 activation via upregulation of Fas expression. We also found that TPL mediated the dissociation of receptor-interacting protein (RIP) from FAK and enhanced the formation of RIP/Fas complex formation initiating cell death. In conclusion, our data firstly demonstrated that TPL induces apoptosis by both extrinsic and intrinsic apoptosis pathways in human melanoma cells and identified that RIP shuttles between Fas and FAK to mediate apoptosis.

8.
Int J Mol Sci ; 13(8): 9642-9648, 2012.
Article in English | MEDLINE | ID: mdl-22949822

ABSTRACT

To examine the neuroprotective effects of Glycine max, we tested its protection against the glutamate-induced toxicity in primary cortical cultured neurons. In order to clarify the neuroprotective mechanism(s) of this observed effect, isolation was performed to seek and identify active fractions and components. From such fractionation, two triterpene glycosides, 3-O-[α-l-rhamnopyranosyl(1-2)-ß-d-glucopyranosyl(1-2)-ß-d-glucuronopyranosyl]olean-12-en-3ß,22ß,24-triol (1) and 3-O-[ß-d-glucopyranosyl(1-2)-ß-d-galactopyranosyl(1-2)-ß-d-glucuronopyranosyl]olean-12-en-3ß,22ß,24-triol (2) were isolated with the methanol extracts with of air-dried Glycine max. Among these compounds, compound 2 exhibited significant neuroprotective activities against glutamate-induced toxicity, exhibiting cell viability of about 50% at concentrations ranging from 0.1 µM to 10 µM. Therefore, the neuroprotective effect of Glycine max might be due to the inhibition of glutamate-induced toxicity by triterpene glycosides.


Subject(s)
Cerebral Cortex/drug effects , Glutamates/toxicity , Glycine max/chemistry , Glycosides/pharmacology , Neurons/drug effects , Neuroprotective Agents/pharmacology , Saponins/pharmacology , Triterpenes/pharmacology , Animals , Cells, Cultured , Cerebral Cortex/cytology , Neurons/cytology , Plant Extracts/pharmacology , Rats , Rats, Sprague-Dawley
9.
Nat Prod Commun ; 7(7): 825-6, 2012 Jul.
Article in English | MEDLINE | ID: mdl-22908556

ABSTRACT

In the search for antiproliferative compounds against human cancer cells (A549, SK-OV-3, SK-MEL-2, XF498, HCT15), it was found that the chloroform extracts obtained from the whole plant of Carpesium rosulatum Miq. (Compositae) exhibited significant cytotoxic activity. Two sesquiterpene lactones, CT-1 (2alpha,5-epoxy-5,10-dihydroxy-6-angeloyloxy-9beta-isobutyloxy-germacran-8alpha, 12-olide), and CT-2 (2beta,5-epoxy-5,10-dihydroxy-6alpha,9beta-diangeloyloxy-germacran-8alpha,12-olide) were isolated from the whole plant. CT-2 showed the most potent cytotoxicity with an IC50 value of 7.88 microM against SK-MEL-2.


Subject(s)
Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/pharmacology , Asteraceae/chemistry , Cell Line, Tumor , Cell Proliferation/drug effects , Chromatography, High Pressure Liquid , Humans , Inhibitory Concentration 50 , Molecular Structure , Republic of Korea
10.
Article in English | MEDLINE | ID: mdl-22911495

ABSTRACT

Oleifolioside A, a new triterpenoid compound isolated from Dendropanax morbifera Leveille (D. morbifera), was shown in this study to have potent inhibitory effects on lipopolysaccharide (LPS-)stimulated nitric oxide (NO) and prostaglandin E(2) (PGE(2)) production in RAW 264.7 macrophages. Consistent with these findings, oleifolioside A was further shown to suppress the expression of LPS-stimulated inducible nitric oxide synthase (iNOS) and cyclooxigenase-2 (COX-2) in a dose-dependent manner at both the protein and mRNA levels and to significantly inhibit the DNA-binding activity and transcriptional activity of NF-κB in response to LPS. These results were found to be associated with the inhibition of the degradation and phosphorylation of IκB-α and subsequent translocation of the NF-κB p65 subunit to the nucleus. Inhibition of NF-κB activation by oleifolioside A was also shown to be mediated through the prevention of p38 MAPK and ERK1/2 phosphorylation. Taken together, our results suggest that oleifolioside A has the potential to be a novel anti-inflammatory agent capable of targeting both the NF-κB and MAPK signaling pathways.

11.
Molecules ; 17(5): 5945-51, 2012 May 18.
Article in English | MEDLINE | ID: mdl-22609785

ABSTRACT

Paulownia coreana has traditionally been used as the medicine and health food in the treatment of cancer and infectious diseases. In the present study, a new antiproliferation agent, isoatriplicolide tiglate (PCAC) was isolated from the chloroform soluble fraction of the leaves of Paulownia coreana. The antiproliferation activities of PCAC plant extract was examined in breast and cervical cancer cell lines in a time-and dose-dependent manners. Our in vitro experiments showed that PCAC suppresses the cell growth and proliferation of cancer cells at a relatively low concentration (< 10 µg/mL) and induces apoptosis at a high concentration (> 50 µg/mL). Western blot analysis showed that concentration higher than 50 µg/mL induces a time-dependent increase in the percentage of apoptotic cells. In this case, PCAC uses both extrinsic and intrinsic pathways for the apoptosis. PCAC treatment decreased the expression of pro-caspase 8, 9, and 3, the main regulators of apoptotic cell death, in MDA-MB-231 cells, accompanied by the activation of caspase 8, 9, and 3. More importantly, PCAC inhibited the in vitro proliferation of six other human breast and cervical cancer cell lines. In conclusion, our data strongly suggest that PCAC acts as an antiproliferation agents particularly against breast and cervical cancers by inducing cell cycle arrest in the S/G2 phase and caspase dependent apoptosis at relatively low (< 10 µg/mL) and high (> 50 µg/mL) concentrations, respectively.


Subject(s)
Ferns/chemistry , Plant Extracts/pharmacology , Sesquiterpenes/pharmacology , Apoptosis/drug effects , Cell Cycle/drug effects , Cell Line, Tumor , Cell Proliferation/drug effects , Humans , Plant Extracts/chemistry , Sesquiterpenes/chemistry
12.
J Agric Food Chem ; 60(21): 5400-6, 2012 May 30.
Article in English | MEDLINE | ID: mdl-22564025

ABSTRACT

Apoptosis, the main type of programmed cell death, plays an essential role in a variety of biological events. Whereas "classical" apoptosis is dependent on caspase activation, caspase-independent death is increasingly recognized as an alternative pathway. To develop new anticancer agents, oleifolioside A was isolated from Dendropanax morbifera Leveille and the biochemical mechanisms of oleifolioside A-induced apoptosis in HeLa cells were investigated. Exposure to oleifolioside A resulted in caspase activation and typical features of apoptosis, although cell death was not prevented by caspase inhibition. Oleifolioside A treatment induced up-regulation of Bad, loss of mitochondrial membrane potential, nuclear relocation of mitochondrial factors, apoptosis-inducing factor (AIF), endonuclease G (EndoG), and apoptosis induction. This is the first report of anticancer activity of oleifolioside A, and nuclear translocation of AIF and EndoG in oleifolioside A-treated HeLa cells might represent an alternative death signaling pathway in the absence of caspase activity.


Subject(s)
Apoptosis Inducing Factor/metabolism , Apoptosis/drug effects , Araliaceae/chemistry , Cell Nucleus/metabolism , Endodeoxyribonucleases/metabolism , Plant Extracts/pharmacology , Saponins/pharmacology , Uterine Cervical Neoplasms/metabolism , Caspases/metabolism , Cell Nucleus/enzymology , Female , HeLa Cells , Humans , Protein Transport , Uterine Cervical Neoplasms/drug therapy , Uterine Cervical Neoplasms/enzymology , Uterine Cervical Neoplasms/physiopathology
13.
Biochem Biophys Res Commun ; 421(2): 190-6, 2012 May 04.
Article in English | MEDLINE | ID: mdl-22503685

ABSTRACT

The expression of matrix metalloproteinase (MMPs)-9 is critical for cell migration and can lead to invasion and metastasis of cancer cells. In the present study, we examined the inhibitory effects of JNP3, a new compound which was isolated from traditional Chinese medicine, on cell invasion and MMP-9 activation in phorbol myristate acetate (PMA)-induced MCF-7 cells. Treatment with JNP3 significantly and selectively inhibited PMA-induced MMP-9 secretion, mRNA expression and protein levels, and these results led to reduction of cell invasion and migration in PMA-induced MCF-7 cells. The results of MMP-9 promoter assay and EMSA showed that JNP3 specifically inhibited PMA-induced MMP-9 gene expression by blocking NF-κB-dependent transcriptional activity. In addition, PMA-induced phosphorylation of ERK1/2 and JNK were suppressed by JNP3 treatment, whereas the phosphorylation of p38 MAPK was not affected by JNP3. These results suggest that JNP3 can be potential anti-cancer agents through specific inhibition of NF-κB-dependent MMP-9 gene expression.


Subject(s)
Antineoplastic Agents/pharmacology , Breast Neoplasms/metabolism , Breast Neoplasms/pathology , Matrix Metalloproteinase Inhibitors , NF-kappa B/antagonists & inhibitors , Triterpenes/pharmacology , Cell Line, Tumor , Down-Regulation , Female , Gene Expression/drug effects , Gene Expression Regulation, Enzymologic/drug effects , Gene Expression Regulation, Neoplastic/drug effects , Humans , MAP Kinase Kinase 4/metabolism , MAP Kinase Signaling System/drug effects , Matrix Metalloproteinase 9/genetics , Neoplasm Invasiveness , Phosphorylation/drug effects , Tetradecanoylphorbol Acetate/pharmacology , Triterpenes/chemistry
14.
Immunopharmacol Immunotoxicol ; 34(1): 95-7, 2012 Feb.
Article in English | MEDLINE | ID: mdl-21612564

ABSTRACT

The study evaluated the anticomplement activity from various solvent extracts of eight Artemisia plants (Artemisia capillaris Thunb., Artemisia fukudo Makino., Artemisia japonica Thunb., Artemisia montana (Nakai) Pamp., Artemisia keiskeana Miq., Artemisia rubripes Nakai., Artemisia stolonifera (Maxim.) Kom., and Artemisia sylvatica Max.) from South Korea on the classical pathway (CP). We have evaluated various organic solvent extract from eight Artemisia plants with regard to its anticomplement activity on the CP. A. rubripes and A. montana chloroform extracts showed inhibitory activity against complement system with 50% inhibitory concentrations (IC50) values of 54.3 and 64.2 µg/mL. This is the first report of anticomplement activity from Artemisia plants.


Subject(s)
Artemisia/chemistry , Complement Pathway, Classical/drug effects , Complement System Proteins/metabolism , Plant Extracts/pharmacology , Animals , Humans , Plant Extracts/chemistry , Rabbits , Republic of Korea , Sheep , Solvents/chemistry
15.
Immunopharmacol Immunotoxicol ; 34(1): 12-4, 2012 Feb.
Article in English | MEDLINE | ID: mdl-21506692

ABSTRACT

The study evaluated the anticomplement effects from organic solvent extracts of five Compositae plants (Ligularia fischeri (Ledeb.) Turez, Ligularia taquetii (H.Lev. & Vaniot) Nakai, Ainsliaea acerifolia Sch.Bip, Aster scaber Thunb, Aster koraiensis Nakai, Synurus deltoides Aiton) from South Korea on the classical pathway complement system. We have evaluated organic solvent extracts from five Compositae with regard to its anticomplement activity. Chloroform extracts from L. taquetii showed inhibitory activity against complement system with 50% inhibitory concentrations (IC50) values of 73.2 µg/mL. This is the first report of anticomplement activity from L. taquetii.


Subject(s)
Asteraceae/chemistry , Complement Pathway, Classical/drug effects , Methanol/chemistry , Plant Extracts/pharmacology , Solvents/chemistry , Animals , Dose-Response Relationship, Drug , Humans , Plant Extracts/chemistry , Rabbits , Sheep
16.
Immunopharmacol Immunotoxicol ; 34(1): 113-5, 2012 Feb.
Article in English | MEDLINE | ID: mdl-21623706

ABSTRACT

The study evaluated the anticomplement activity from isolated compounds from Artemisia montana (Nakai) Pamp. from South Korea on the classical pathway. In a previous work, A. montana (Nakai) Pamp. chloroform extracts showed inhibitory activity against complement system. The chromatographic separation of a chloroform chloride extract of A. montana (Nakai) Pamp. led to the isolation of four compounds. Their structures were characterized to be ezoartemin, yamayomoginin, ezomontanin and 11,13-dihydroezomontanin by spectroscopic data. This is the first report of anticomplement activity of isolated compounds from A. montana (Nakai) Pamp.


Subject(s)
Artemisia/chemistry , Complement Pathway, Classical/drug effects , Complement System Proteins/chemistry , Adult , Animals , Humans , Male , Sheep
17.
Immunopharmacol Immunotoxicol ; 34(2): 213-5, 2012 Apr.
Article in English | MEDLINE | ID: mdl-21770839

ABSTRACT

The present study evaluated the anticomplement effects of isolated compounds from Achyranthes japonica in the classical pathway of the complement system. Using column chromatography, three compounds: achyranthoside C dimethyl ester, achyranthoside C butyl dimethyl ester, and achyranthoside E dimethyl ester were isolated and evaluated for in vitro anticomplement activity. Achyranthoside C dimethyl ester showed the most potent inhibitory activity against the complement system, with 50% inhibitory concentrations (IC(50)) values of 26.2 µg/mL. This is the first report of anticomplement activity of isolated compounds from A. japonica.


Subject(s)
Achyranthes/chemistry , Complement Inactivating Agents/pharmacology , Complement Pathway, Classical/drug effects , Oleanolic Acid/analogs & derivatives , Oleanolic Acid/pharmacology , Saponins/pharmacology , Animals , Chloroform/chemistry , Chromatography, High Pressure Liquid , Chromatography, Thin Layer , Complement Inactivating Agents/isolation & purification , Complement Pathway, Classical/immunology , Hemolysis/immunology , Inhibitory Concentration 50 , Oleanolic Acid/isolation & purification , Plant Extracts/chemistry , Plant Extracts/isolation & purification , Rabbits , Saponins/isolation & purification , Sheep
18.
Immunopharmacol Immunotoxicol ; 34(2): 244-6, 2012 Apr.
Article in English | MEDLINE | ID: mdl-21854097

ABSTRACT

The study evaluated the anticomplement activity from isolated compounds from Artemisia rubripes Nakai from South Korea on the classical pathway. In the previous works, Artemisia rubripes chloroform extracts showed inhibitory activity against complement system. The chromatographic separation of a chloroform chloride extract of Artemisia rubripes led to the isolation of three compounds. Their structures were characterized to be scopoletin (1), 11,(13)-triene-6,12-olide (2), and 1ß,6α-dihydroxy-4(15)-eudesmene (3) by spectroscopic data. This is the first report of anticomplement activity of isolated compounds from Artemisia rubripes.


Subject(s)
Artemisia/chemistry , Complement Inactivating Agents/pharmacology , Complement Pathway, Classical/drug effects , Scopoletin/pharmacology , Sesquiterpenes/pharmacology , Animals , Chloroform/chemistry , Chromatography, High Pressure Liquid , Chromatography, Thin Layer , Cinnamates/pharmacology , Complement Inactivating Agents/chemistry , Complement Inactivating Agents/isolation & purification , Depsides/pharmacology , Flavonoids/pharmacology , Hemolysis/immunology , Inhibitory Concentration 50 , Molecular Structure , Plant Extracts/chemistry , Rabbits , Scopoletin/chemistry , Scopoletin/isolation & purification , Sesquiterpenes/chemistry , Sesquiterpenes/isolation & purification , Sesquiterpenes, Eudesmane/chemistry , Sesquiterpenes, Eudesmane/isolation & purification , Sesquiterpenes, Eudesmane/pharmacology , Sheep , Rosmarinic Acid
19.
Immunopharmacol Immunotoxicol ; 34(2): 299-302, 2012 Apr.
Article in English | MEDLINE | ID: mdl-21854169

ABSTRACT

The present study evaluated the anticomplement effects from isolated compounds of Sorghum bicolor in classical pathway complement system. Using column chromatograph, three compounds; Sorgoleone-362 (1), Sorgoleone-360 (2) and Sorgoleone-386 (3) were isolated and evaluated for in vitro anticomplement activity. Sorgoleone-386 showed inhibitory activity against complement system with 50% inhibitory concentrations (IC(50)) values of 148.3µg/ml. This is the first report of anticomplement activity of isolated compounds from Sorghum bicolor.


Subject(s)
Benzoquinones/pharmacology , Complement Inactivating Agents/pharmacology , Complement Pathway, Classical/drug effects , Lipids/pharmacology , Plant Extracts/chemistry , Plant Roots/chemistry , Sorghum/chemistry , Animals , Benzoquinones/isolation & purification , Chromatography, High Pressure Liquid , Chromatography, Thin Layer , Cinnamates/pharmacology , Complement Inactivating Agents/isolation & purification , Depsides/pharmacology , Flavonoids/pharmacology , Hemolysis/immunology , Inhibitory Concentration 50 , Lipids/isolation & purification , Molecular Structure , Rabbits , Sheep , Rosmarinic Acid
20.
Immunopharmacol Immunotoxicol ; 34(2): 265-74, 2012 Apr.
Article in English | MEDLINE | ID: mdl-21854183

ABSTRACT

This study is aimed to evaluate the protective effect of fermented Angelicae gigantis Radix (AGR) with Monascus purpureus strain on carbon tetrachloride (CCl(4))-induced hepatotoxicity and oxidative stress in rats. The activities of liver marker enzymes, alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and the levels of lipid peroxidation were increased when CCl(4) was treated but these parameters were significantly decreased by fermented AGR treatment. CCl(4) treatment exhibited decrease in serum concentrations of triglyceride, total cholesterol, HDL-cholesterol, and free fatty acids, and these were also decreased by fermented AGR administration. The level of serum leptin was significantly lower in fermented AGR administration than that in normal control group. CCl(4) treatment significantly increased the concentration of liver triglyceride. The current study observed significant elevations of the thiobarbituric acid-reactive substances (TBARS) levels in the liver homogenate, mitochondrial, and microsomal fractions of CCl(4) control group compared with normal control group. CCl(4) treatment resulted in a significant decrease in the levels of plasma and hepatic glutathione, but these reductions were significantly increased by fermented AGR administration. CCl(4) induced the marked hepatocytes necrosis and fatty accumulation around the central veins. Accordingly, fermented AGR may be an ideal candidate for the hepatoprotective effect in animal model.


Subject(s)
Angelica/chemistry , Carbon Tetrachloride/pharmacology , Chemical and Drug Induced Liver Injury/prevention & control , Fermentation , Oxidative Stress/drug effects , Phytotherapy/methods , Plant Extracts/therapeutic use , Adipose Tissue, White/drug effects , Adipose Tissue, White/pathology , Animals , Body Weight/drug effects , Chemical and Drug Induced Liver Injury/blood , Chemical and Drug Induced Liver Injury/metabolism , Chemical and Drug Induced Liver Injury/pathology , Coumarins/analysis , Coumarins/metabolism , Drinking/drug effects , Eating/drug effects , Enzymes/blood , Glutathione/blood , Glutathione/metabolism , Leptin/blood , Lipids/blood , Liver/drug effects , Liver/enzymology , Liver/metabolism , Liver/pathology , Male , Microsomes, Liver/drug effects , Microsomes, Liver/metabolism , Mitochondria, Liver/drug effects , Mitochondria, Liver/metabolism , Monascus/metabolism , Necrosis/pathology , Necrosis/prevention & control , Organ Size/drug effects , Plant Extracts/chemistry , Plant Extracts/metabolism , Plant Extracts/pharmacology , Plant Roots/chemistry , Rats , Rats, Sprague-Dawley , Thiobarbituric Acid Reactive Substances/metabolism , Zinc/blood
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