Curcumin loaded liposome formulation: Enhanced efficacy on performance, flesh quality, immune response with defense against Streptococcus agalactiae in Nile tilapia (Orechromis niloticus).

Application of novel trend comprising antioxidant phytogenics is aiming to minimize the stress related factors and associated diseases in intensive fish culturing. Today, the concept of exploiting and protecting natural antioxidants represents a paradigm shift for the aqua feed industry. Therefore, our principal goal targeting liposome as a novel nanocarrier for curcumin is directed to attain superior performance, fillet antioxidant stability and bacterial resistance in Nile tilapia. A total of 500 Nile tilapia fingerlings (average body weight, 10.27 ± 0.10 g) assigned into five experimental groups in 25 glass aquaria of 120 L capacity at the density 20 fish/aquaria. The experimental groups were supplemented with varying doses of liposomal curcumin-NPs, LipoCur-NPs (0, 5, 15, 25 and 35 mg/kg diet) were reared for 12 weeks and later Streptococcus agalactiae (S. agalactiae) challenged model was performed. Inclusion of LipoCur-NPs (25 and 35 mg/kg diet) had the most prominent impact on Nile tilapia growth rate and feed conversion ratio. The immune boosting outcomes post supplementing 35 mg/kg diet of LipoCur-NPs were evidenced by higher myeloperoxidase, lysozyme and total immunoglobulin levels. Even after 4 weeks frozen storage, LipoCur-NPs at the dose of 35 mg/kg diet prominently increased (P < 0.05) the fillet scavenging capability for free radicals (1,1-diphenyl-2-picrylhydrazyl and 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) with an inverse reduction in lipid peroxidation biomarker (malondialdehyde). Notably, upregulation of GSH-Px, CAT, and SOD genes in fillet of 35 mg/kg LipoCur-NPs fed fish coordinated with higher T-AOC and lower oxidative markers (ROS and H2O2). Post S. agalactiae challenge, higher supplementation levels of LipoCur-NPs (35 mg/kg diet) greatly attenuated the expression of its vital virulence genes (cfb, fbsA and cpsA) with higher expression of Igm, CXC-chemokine and MHC genes. Concordantly, downregulation of inflammatory markers (IL-1ß, TNF-α and IL-8) and upregulation of anti-inflammatory ones (IL-10 and TGF-ß) were remarkably documented. Based on these findings, the innovative curcumin loaded liposome was considered a novel multitargeting alternative not only playing an imperative role in Nile tilapia growth promotion and fillet stability upon storage, but also protecting efficiently against S. agalactiae.

Dietary Eugenol Nanoemulsion Potentiated Performance of Broiler Chickens: Orchestration of Digestive Enzymes, Intestinal Barrier Functions and Cytokines Related Gene Expression With a Consequence of Attenuating the Severity of E. coli O78 Infection.

Recently, the use of essential oils (EOs) or their bioactive compounds encapsulated by nanoparticles as alternative supplements for in-feed antimicrobials is gaining attention, especially in organic poultry production. Focusing on eugenol, its incorporation into the nanoformulation is a novel strategy to improve its stability and bioavailability and thus augment its growth-boosting and antimicrobial activities. Therefore, we explored eugenol nanoemulsion activities in modulating growth, digestive and gut barrier functions, immunity, cecal microbiota, and broilers response to avian pathogenic E. coli challenge (APEC) O78. A total of 1,000 one-day-old broiler chicks were allocated into five groups; negative control (NC, fed basal diet), positive control (PC), and 100, 250, and 400 mg/kg eugenol nanoemulsion supplemented groups. All groups except NC were challenged with APEC O78 at 14 days of age. The results showed that birds fed eugenol nanoemulsion displayed higher BWG, FI, and survivability and most improved FCR over the whole rearing period. Birds fed 400 mg/kg of eugenol nanoemulsion sustained a higher growth rate (24% vs. PC) after infection. Likely, the expression of digestive enzymes' genes (AMY2A, CCK, CELA1, and PNLIP) was more prominently upregulated and unaffected by APEC O78 challenge in the group fed eugenol nanoemulsion at the level of 400 mg/kg. Enhanced gut barrier integrity was sustained post-challenge in the group supplemented with higher levels of eugenol nanoemulsion as evidenced by the overexpression of cathelicidins-2, ß-defensin-1, MUC-2, JAM-2, occludin, CLDN-1, and FABP-2 genes. A distinct modulatory effect of dietary eugenol nanoemulsion was observed on cytokine genes (IL-1ß, TNF-α, IL-6, IL-8, and IL-10) expression with a prominent reduction in the excessive inflammatory reactions post-challenge. Supplementing eugenol nanoemulsion increased the relative cecal abundance of Lactobacillus species and reduced Enterobacteriaceae and Bacteriods counts. Notably, a prominent reduction in APEC O78 loads with downregulation of papC, iroN, iutA, and iss virulence genes and detrimental modifications in E. coli morphological features were noticed in the 400 mg/kg eugenol nanoemulsion group at the 3rd-week post-challenge. Collectively, we recommend the use of eugenol nanoemulsion as a prospective targeted delivery approach for achieving maximum broilers growth and protection against APEC O78 infection.

Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).

Nowadays there is a great attention for nanotechnology in aquaculture production. It has an efficient role in nutrients and drugs delivery, ponds sterilization, water treatment and aquatic diseases reduction. Till now, there is no available data on impact of selenite-loaded chitosan nanoparticles (SeChNPs) on Nile tilapia. Hence, the current study investigated the effects of selenite-loaded chitosan nanoparticles supplementation on the growth, immune, antioxidant and apoptotic related genes as well as resistance to Aeromonas hydrophila of Nile tilapia, Oreochromis niloticus. A total of 400 fish were randomly divided into four groups, and each group retained five replicates. The control group was fed a basal diet (with inorganic se), other groups fed diets supplemented with SeChNPs 0.5, 1 and 2 g/kg diet. The loading concentration of Se to ChNPs was 0.3, 0.6 and 1.2 mg/0.5, 1 and 2 gm respectively. Fish groups fed SeChNPs (0.5 and 1 g/kg) exhibited the highest final body gain, better feed utilization. Additionally, the expression of myostatin gene was down-regulated by 0.2 and 0.3 fold in group fed 0.5 and 1 g/kg SeChNPs when compared with control group. Dietary inclusion of SeChNPs increased serum lysozyme, alternative complement and myeloperoxidase activities and immunoglobulin type M level. Supplementation of SeChNPs at the level of 2 g/kg up-regulated glutathione peroxidase, superoxide dismutase and catalase expression by 1.12, 4.9 and 2.31 folds respectively, in comparison with control group. In contrast, the levels of C- reactive protein and malondialdehyde were reduced. The expression of IL-10, IL-8, TNF-α and IL-1ß genes was up-regulated after dietary inclusion of different levels of SeChNPs in a dose dependent manner. Post-challenge, the highest survival rate was detected in group fed 2 g/kg SeChNPs (93%) in contrast, the control group was displayed the lowest survival rate (45%). After challenge with A. hydrophila, the expression of caspase 1 was up-regulated in groups fed 1 and 2 g/kg of SeChNPs. Moreover, the maximum down-regulation of cytochromes P450 and heat shock protein were found in 2 g/kg SeChNPs supplemented group (reduced by 0.4 and 0.6-fold, respectively, when compared with control group). In conclusion, the ameliorative effects of SeChNPs on Nile tilapia growth resulted from immune stimulatory and free radicals scavenging effects of selenium loaded chitosan nano composite.

Effects of dietary white mulberry leaves on hemato-biochemical alterations, immunosuppression and oxidative stress induced by Aeromonas hydrophila in Oreochromis niloticus.

The present work was designed to assess the potential hemato-biochemical protective action, immunemodulatory and antioxidant conclusions of varied concentration of white mulberry Morus alba leaves (MAL) extract supplementation on Nile tilapia (Oreochromis .niloticus). A total two hundred and forty of O. niloticus were haphazardly sorted into four groups. The control (CT) group was fed on basal diet. A group MAL1, MAL3 and MAL5 was fed on 1, 3 and 5 g/kg MAL respectively for thirty days. On day thirty one, half of replicates in each group were challenged by 0.5 ml × 108Aeromonas hydrophila where, the residual replicates were kept without challenge. A. hydrophila challenged tilapias revealed anemia that alleviated by supplementation with 5 g/kg MAL also, recovers the shift of leucogram prompted by the challenge. Elevation of alkaline phosphatase, aminotransferases, lactate dehydrogenase and malondialdehyde (ALP, ALT, AST, LDH and MDA) in CT, MAL1 and MAL3 in the challenged replicates respectively where within normal at MAL5. Supplementation with MAL5 showed more potent antioxidant and immune reaction than MAL1 and MAL3. There were a rapid increase of immunoglobulin M, lysozymes, nitric oxide, catalase and superoxide dismutase and their allied genes expression (IgM, CAT and SOD) in MAL groups with contrast in CT challenged groups. Where in challenged groups, there was suppression in genes expression of interleukins (8 and 1 beta) and interferon ɤ (IL8. IL-1ß and INFɤ). Tilapias challenged by A. hydrophila unveiled plentiful surge in the percentage of mortality in CT challenged fish (80%), followed by the groups supplemented with MAL1 and MAL3 were (73.33%) where MAL5 was 20%. The mortalities have been halted from the 6th, 13th, 14th and 15th days in, MAL5, MAL3, MAL1, and CT correspondingly. These previous results could be fulfilled that using of MAL 5 g/kg protect tilapias from hemato-biochemical alterations and enhance its immune feedback, antioxidant defense and resistance against A. hydrophila.

Effect of Chlorella vulgaris enriched diet on growth performance, hemato-immunological responses, antioxidant and transcriptomics profile disorders caused by deltamethrin toxicity in Nile tilapia (Oreochromis niloticus).

The toxic effect of deltamethrin (DM) was documented in aquaculture. There is no obtainable data on the effect of Chlorella vulgaris against DM toxicity. The current study focused on the effect of dietary supplementation with C. vulgaris (CV) on growth performance, innate immune response, antioxidant activities, and transcriptomics disorders induced by sub-lethal dose of DM in Oreochromis niloticus. A total number of 216 O. niloticus divided into four groups with tri-replicates. The 1st control group (CT) fed a basal diet, the second group fed diet enriched with 5% CV. The third group was exposed to DM (15 µg/L), where the last group fed CV and simultaneously exposed to DM as previous-mentioned. The procedure of CV feeding and DM exposure were continued for two months. Exposures to DM revealed in stunting of the growth parameters and lessening of survival ratio of tilapias with a significant decline of the erythrogram (macrocytic hypochromic anemic picture), and leucocytes immune cells and related parameters (immunoglobulin M, lysozyme) and sever shifting in the antioxidant indicators. Sever raise was monitored in hepatic and kidney markers. Also, genes expression related to immune and antioxidant parameters were severely impacted. Where tilapias received CV showed a significant increase in the growth and immune parameters besides to an improvement of hematological, antioxidant values and their related genes expressions. The fourth group that received CV simultaneous with DM exposure showed a soothing of the previous indicators and markers toward the values of tilapias fed on basal diet (CT). In turn, CV supplementation may be presented a protective effect alongside DM toxicity in O. niloticus appeared through soothing of the immune, antioxidant and related genes expressions in addition to its hepato-renal protective effects. Therefore, the current study recommended that an incorporating of 5% CV for tilapias diet could improve their growth performance, immunity, antioxidant and transcriptomics disorders induced by deltamethrin.

Impact of dietary supplementation with Echinacea purpurea on growth performance, immunological, biochemical, and pathological findings in broiler chickens infected by pathogenic E. coli.

This study is aimed to appraise the impact of dietary supplementation with Echinacea purpurea (EP) in broiler chickens infected by pathogenic E. coli. Eighty-one-day-old chicks of both sexes, commercial Hubbard chicks, were divided into 4 groups. The first was kept as normal control, the second was supplemented by EP (5 mg/kg feed) from the first day to the end, where the third was infected orally by 1 ml E. coli O78 at 107 CFU intra-crop at 7 days old, while the last group was supplemented by EP and infected by the E. coli O78 as previously described . E. coli-infected chicks disclosed severe symptoms with mortality 30%. Where, the infected and EP supplemented group showed less clinical symptoms and 15% mortality. EP-supplemented groups showed a significant improvement in their body weight in comparing with infected group. E. coli-infected chickens revealed a significant drop in lymphocytes' number with a significant elevation in monocyte and heterophils with non-significant changes in total leucocyte count when matched with gp. (1), while the fourth group revealed non-significant changes in total leucocyte with a significant drop in heterophils and monocyte and a significant elevation in the lymphocytes matching with the 3rd group. Serum biochemical markers of liver, kidneys, and immunological parameters showed sever adverse alterations associated with E. coli infection. Dietary supplementations with Echinacea purpurea improve the final body weight and immune response of non-infected chicks and have a respectable effect for facing E. coli infection. EP improves hematological and serum biochemical adverse effects occured by E. coli infection. Histopathology of liver, kidneys, intestines, and spleen confirms these alterations.

Immune-protective, antioxidant and relative genes expression impacts of ß-glucan against fipronil toxicity in Nile tilapia, Oreochromis niloticus.

Our study is considered to attempt reducing the immune-toxic and antioxidant impacts of exposure to fipronil (FP) on Nile tilapia, Oreochromis niloticus using the ß-glucan (ßG). Two hundred and seventy fingerlings of Nile tilapia were divided randomly into six groups (45 tilapias of each, in 3 replicates): group I control (CT) group nourished on a basal diet. Group II (ßG) nourished a basal diet supplemented with 0.4% ßG. Groups III (1/20 FP) and V (1/10 FP) was exposed to 1/20 and 1/10 of the 96 h LC50 of FP in water and nourished the basal diet respectively. Groups IV (1/20 FP+ ßG) and VI (1/10 FP+ ßG) were exposed to 1/20 and 1/10 FP concomitantly with 0.4% ßG supplementation for 90 successive days. Growth performance metrics were higher in ßG group than CT. While those metrics were fallen at exposure to 1/20 or 1/10 FP. Supplementation with ßG elevated the IgM and lysozyme levels.Whereas, tilapias exposed to FP only at different concentration showed lowering of those compared to CT. Supplementation with ßG was effectively augmented IgM and lysozyme in 1/20 FP exposed tilapias. Furthermore, in a minor grade at 1/10 FP exposed tilapias. Exposure to FP increased the activities of hepatic markers chiefly at 1/10, however the ßG supplementation was successfully improved these markers. There was imbalance of cortisol level at FP exposure where, ßG combining to FP alleviate this disparity. There was fallen in LDH, MDH and FDPase in ßG tilapias where continuing raise in 1/10 FP followed by 1/20 FP. ßG supplementation raise the level of GSH, without significant variations in MDA conversely occurs in FP alone. Genes expression of ßG caused raise of both GPx and GR, without fluctuations in CAT and SOD. Exposure to FP diminishes all evaluated antioxidant genes. It could fulfilled that supplementation with ßG successfully alleviated the immune-toxic and antioxidant impact of FP in tilapias.

Efficacy of dietary Nano-selenium on growth, immune response, antioxidant, transcriptomic profile and resistance of Nile tilapia, Oreochromis niloticus against Streptococcus iniae infection.

As recently applicable, there are few studies on the impact of using nano-selenium (nano-Se) on varied fish species. Where nothing reachable focused on its impact on tilapias so, the present analysis evaluated the efficacy of using nano-Se in tilapias on immune response, antioxidant defense compared by conventional Se form. 480 O. niloticus fingerlings were haphazardly grouped firstly into three groups with four replicates of each. The control one (CT) was fed on a basal diet. The second and third one supplemented with 0.7 mg/kg-1 Se and nano-Se respectively for ten weeks. At the start day of the ninth week, two replicates from each group were injected by Streptococcus iniae where, the remaining replicates stand without challenge. Enhancement of growth performance measurements were noted in nano-Se compared to Se or CT groups. Existed anemia in S. iniae tilapias became alleviated by using nano-Se that also, improves the alteration of leucogram induced by challenge. Elevation of aminotransferases, alkaline phosphatase, lactate dehydrogenase (ALT, AST, ALP and LDH) and creatinine in Se and CT challenged replicates that seemed nearly normal by using nano-Se. Usage of nano-Se showed more powerful antioxidant activities than Se. There were an expansion of immunoglobulin M, lysozymes, glutathione peroxidase, nitric oxide, superoxide dismutase and catalase (IgM, LYZ, GPx, NO, SOD, CAT) and their related gene expression in nano-Se with contrast in Se or CT challenged groups. Nile tilapias challenged by S. iniae disclosed substantial expansion in the percentage of mortality in CT challenged fish (93.33%), followed by the group supplemented with Se (73.33%), whereas the lowermost one at fish supplemented by nano-Se (26.66%). The mortalities have been stopped from the 5th, 12th and 14th days in, nano-Se, Se and CT respectively. It can be concluded that using of Se 0.7 mg/kg-1induce immunosuppressive, antioxidant, liver and kidneys negative impact on tilapias where the same dose from nano-Se was more potent immunomodulating and antioxidant. Also it is attend in counteracting the serious impact induced by S. iniae challenge.

Anti-tumour effects of Egyptian propolis on Ehrlich ascites carcinoma.

A total of 150 female Swiss mice were used to study the ability of water soluble propolis derivatives (WSPD) of Egyptian propolis to inhibit the proliferation and growth of Ehrlich ascites carcinoma (EAC) cells in mice. The mice were divided equally into three groups: the first was kept as a negative control group, the second received an intraperitoneal injection of 2.5 × 10(6) EAC and was kept as a positive control group and the third an intraperitoneal injection of 2.5 × 10(6) EAC and treated with propolis (50 mg/kg body weight) administered by gastric intubations 2 h prior to the intraperitoneal injection of EAC. The propolis was administered daily for 11 successive days. An examination of EAC cells revealed a reduction in the volume, total cell count, viable percentage and increase in the percentage of dead cells in the treated group with an increasing mean survival time (MST), increasing life span (ILS) percentage and treated vs positive control (T/C) percentage. Immunological studies revealed a significant increase in the lymphocyte transformation rate (LTR), phagocytic activity and killing power in the group treated with propolis. A haematological study of the parameters revealed leucocytosis in cancer-bearing mice and propolis-treated groups with granulocytosis and monocytosis. The erythrogram revealed a significant reduction in red blood cell (RBC) count in group 2. The result showed that the implantation of EAC in Swiss mice without treatment resulted in a significant decrease in total protein and albumin levels without a change in globulin level and a significant increase in creatinine level, while the third group that received propolis showed an improvement in these biochemical parameters compared to the normal control group.