[The ultrastructure of Leydig cells under the influence of drinking mineral water and electromagnetic radiation under the stress conditions in the rats]. / Ul'trastruktura kletok Leidiga pri deistvii mineral'noi vody i nizkointensivnogo elektromagnitnogo izlucheniya v usloviyakh stressa u krys.

The objective of the present study was elucidate the peculiar features of low-intensity electromagnetic radiation (LI EMR) and mineral water (MW) on the ultrastructure of rat Leydig cells under conditions of immobilization stress. The experiments were carried out on outbred male rats with the use of electron microscopy. It has been demonstrated that the prophylactic consumption of drinking sulfate-containing mineral water and the application low-intensity electromagnetic radiation (with the flow power density of 1 mcW/cm2 and frequency around 1,000 Hz) or the combination of these two modalities under conditions of immobilization stress reduced the degree of ultrastructural derangement in the rat Leydig cells and stimulated the development of regenerative processes. In the cases of the single-factor impact, drinking mineral water exerted more pronounced action than low-intensity electromagnetic radiation on mitochondrial regeneration. In case of the simultaneous application of the two factors their protective action on the Leydig cells was much more conspicuous than that of either of them applied alone. It is concluded that drinking sulfate-containing mineral water in combination with the application of low-intensity electromagnetic radiation enhances resistance of the rat Leydig cells to stress.

[The ultrastructural manifestations of the regenerative processes in the Sertoli cells under the action of low-intensity electromagnetic radiation in the rats subjected to stress].

The experiments on the outbred female rats using the electron microscopic technique have demonstrated that the application of ultrahigh frequency low-intensity electromagnetic radiation (LIEMR) with a flux density below 1 mCW/Cm2 and a frequency of approximately 1,000 MHz in the regime of primary prophylaxis and therapeutic-preventive action suppressed the development of the post-stress pathological ultrastructural changes and increased the activity of the regenerative processes in the Sertoli cells. It was shown that the developing adaptive and compensatory changes in the Sertoli cells most frequently involve the energy-producing structures (mitochondria) that undergo the enlargement of their average and total dimensions. Simultaneously, the amount of granular endoplasmic reticulum and the number of ribosomes increased while the intracellular links between the organelles strengthened and the reserve potential of the cells improved. It is concluded that the observed effects may be due to the action of both local and systemic regulation mechanisms.

[The regeneration process in the Sertoli cells and Leydig cells of the rats undergoing the combined treatment with drinking mineral water and a magnetic field under stressful conditions].

The experiments carried out on outbred white male rats have demonstrated that twice repeated immobilization stress resulted in the destruction of intracellular organelles and interfered with the regeneration processes in the Sertoli cells and Leydig cells. The combination of the consumption of drinking mineral sulfate water (MW) with the application of a constant or alternating magnetic field (MF) caused stimulation of intracellular regenerative reactions that were especially pronounced in the Sertoli cells in the form of enhanced organoid and intra-organoid regeneration of mitochondria. This in turn increased biological potential of the cells and thereby provided a basis for the further development of biosynthetic processes. The results of the study expose to a certain degree the causes and mechanisms responsible for the higher effectiveness of the combined action of mineral water and magnetic fields compared with the monofactor treatment (mineral water alone).

[Ultrastructural changes of sertoli cells in rat testicles treated with drinking mineral water in combination with microelements, zinc and silicon, under stress conditions].

The present experiments carried out on outbred male rats have demonstrated that the treatment with drinking mineral water in combination with microelements, zinc and silicon, significantly increased resistance of their cultured Sertoli cells to the stressful impact of a single immobilization event and promoted the development of intracelular adaptive and protective reactions. This response was manifest as a decrease in the level of the destructive processes and an enhancement of regenerative/hyperplastic reactions of mitochondria, ribosomes, and other intracellular organelles. Similar experiments with the use of sulfate mineral water revealed its less pronounced effect.

[Prevention of early post-stress disorders in rat testicles under effect of drinking sulfate mineral water containing zinc and silicium].

Experiments performed on outbred male rats have demonstrated that consumption of drinking sulfate mineral water containing microelements (zinc and silicium) considerably slows down the development of stress-induced pathological changes in the testicles compared with the action of pure mineral water containing no admixtures. This finding confirms the important role of zinc and silicon in combination with the ion-salt base of mineral water in the enhancement of the efficacy of the adaptive and protective reactions in the testicles during the early post-stress period.

[The use of drinking sulfate mineral water in combination with laser and magnetic-laser irradiation for primary prophylaxis of post-radiation disorders(experimental study)].

Experiments on laboratory rats have demonstrated that prophylactic use of drinkable sulfate mineral water in combination with laser and magnetolaser irradiation of adrenal glands produces a radioprotecive effect that was especially well apparent in the liver and less so in the testes. Simultaneously, the functional activity of the thymus decreased. Protection of the liver by the combination of the above factors was more pronounced than the effect of each of them applied separately.

[Spermatogenesis in rats given potable sulfated mineral water in the early postradiation period]. / Sostoianie spermatogeneza u krys pri primenenii pit'evoi sul'fatnoi mineral'noi vody v rannem postradiatsionnom periode.

Rat experiments have shown that course intake (21 days) of sulphate sodium-magnesium-calcium water with sulphate concentration 2.6 mg/l and mineralization 3.1 g/l in early postradiation period (1-2 Gy) inhibits development of dystrophic and destructive processes in spermatogenic epithelium of the testes and maintains spermatogenesis at higher level than control.

[The dependence of the radioprotective effect of iodine-containing waters on the concentration and form of the iodine]. / Zavisimost' radiozashchitnogo éffekta iodsoderzhashchikh vod ot kontsentratsii i formy ioda.

Preradiation intragastric injection of iodine-containing waters during 21 days increased mean survival and decreased the mortality of rats exposed to gamma-radiation (whole-body dose 5.4 and 6.4 Gy, dose rate 1.053 Gy/min). The highest radioprotective effect was reached in use of waters containing 49 and 100 mg/1 positive iodine ions in radiation dose 5.4 Gy. In the whole-body radiation 6.4 Gy, intragastric injection of water with 49 mg/1 positive ions resulted in 100% survival.

[The action of sulfate mineral water in experimental whole-body irradiation]. / Deistvie sul'fatnoi mineral'noi vody pri obshchem radiatsionnom obluchenii v éksperimente.

The authors' experiments have shown that a course intake of sulfate mineral water after total radiation (1 and 5 Gy) can prevent and minimize dystrophic and necrobiotic changes in the liver, stimulate intracellular regeneration, reduce pathological mitoses in the small intestine. The response depended on radiation dose and time of crenotherapy start after radiation. Sulfate mineral water is also a weak radioprotector.

[The postradiation restoration of the liver by using potable mineral waters]. / Postradiatsionnoe vosstanovlenie pecheni pri primenenii pit'evykh mineral'nykh vod.

In experiments with 135 white breed-less male rats it was shown that the drinking mineral waters containing sulphate or silic ions promoted the postradiated rehabilitation in the liver after whole-body 5 Gy gamma-ray exposure. The decrease of the rate of lipid peroxidation in hepatocyte membranes, the increase of quantity normal cells and the decrease of quantity dystrophic and necrobiotic cells, the change of haemostasis took place. The iodine- and boron-containing mineral waters were less efficacious in these processes than the waters with sulphate and silic ions.

[Postradiation recovery processes in the body with the internal use of iodine-containing waters]. / Postradiatsionnye vosstanovitel'nye protsessy v organizme pri vnutrennem primeneniem iodsoderzhashchikh vod.

It is shown that both natural and dissolved iodine-bromine water given in courses to animals preexposed to 1 Gy gamma radiation promotes recovery of the damaged processes in the body. The therapeutic action of natural mineral water proved more beneficial because such water contains more iodine and minerals.

[The characteristics of the action of potable mineral waters on the postradiation recovery processes in rats receiving different doses of gamma irradiation]. / Osobennosti deistviia pit'evykh mineral'nykh vod na postradiatsionnye vosstanovitel'nye protsessy u krys pri raznykh dozakh gamma-oblucheniia.

In experiments on 54 white noninbred male rats it was shown that drinking mineral water containing sulphate, calcium and sodium ions (Moskovskaia mineral water) activates postradiation regenerative processes in the thymus and the liver and normalizes functional activity of CCS-system in blood plasma if it was taken 28 days after whole-body 1 Gy gamma-radiation. This positive effect was less marked after 5 Gy gamma-radiation. Silicon and boron containing mineral water under the above conditions proved less effective.