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Therapeutic Methods and Therapies TCIM
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PLoS One ; 16(3): e0235454, 2021.
Article in English | MEDLINE | ID: mdl-33661896

ABSTRACT

BACKGROUND AND AIMS: Inflammation and calcification are major factors responsible for degeneration of bioprosthetic valve and other substitute heart valve implantations. The objective of this study was to evaluate the anti-inflammatory and anti-calcification effects of Entelon150® (consisting of grape-seed extract) in a beagle dog model of intravascular bovine pericardium implantation. METHODS: In total, 8 healthy male beagle dogs were implanted with a bovine pericardium bilaterally in the external jugular veins and divided into two groups. Animals in the Entelon150® group (n = 4) were treated with 150 mg of Entelon150® twice daily for six weeks after surgery. The negative control (NC) group (n = 4) was treated with 5 ml of saline using the same method. After six weeks, we measured the calcium content, performed histological examination, and performed molecular analysis. RESULTS: The calcium content of implanted tissue in the Entelon150® group (0.56±0.14 mg/g) was significantly lower than that in the NC group (1.48±0.57 mg/g) (p < 0.05). Histopathological examination showed that infiltration of chronic inflammatory cells, such as fibroblasts and macrophages, occurred around the graft in all groups; however, the inflammation level of the implanted tissue in the Entelon150® group was s lower than that in the NC group. Both immunohistochemical and western blot analyses revealed that bone morphogenetic protein 2 expression was significantly attenuated in the Entelon150® group. CONCLUSIONS: Our results indicate that Entelon150® significantly attenuates post-implantation inflammation and degenerative calcification of the bovine pericardium in dogs. Therefore, Entelon150® may increase the longevity of the bovine pericardium after intravascular implantation.


Subject(s)
Anti-Inflammatory Agents/therapeutic use , Calcinosis/drug therapy , Grape Seed Extract/therapeutic use , Postoperative Complications/drug therapy , Transcatheter Aortic Valve Replacement/methods , Animals , Anti-Inflammatory Agents/administration & dosage , Anti-Inflammatory Agents/pharmacology , Bioprosthesis , Calcinosis/etiology , Cattle , Dogs , Fibroblasts/drug effects , Grape Seed Extract/administration & dosage , Grape Seed Extract/pharmacology , Heart Valve Prosthesis , Macrophages/drug effects , Male , Pericardium/transplantation , Transcatheter Aortic Valve Replacement/adverse effects
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