ABSTRACT
This study proposes an alternative technique to prevent heat degradation induced by classic procedures of bioactive compound extraction, comparing classical maceration/decoction in hot water of polyphenols from Mango (Mangifera indica L.) (MI) with ultrasound-assisted extraction (UAE) in a water solution of ß-cyclodextrin (ß-CD) at room temperature and testing their biological activity on TNFα-induced endothelial dysfunction. Both extracts counteracted TNFα effects on EAhy926 cells, down-modulating interleukin-6, interleukin-8, cyclooxygenase-2 and intracellular adhesion molecule-1, while increasing endothelial nitric oxide synthase levels. ß-CD extract showed higher efficacy in improving endothelial function. These effects were abolished after pre-treatment with the oestrogen receptor inhibitor ICI1182,780. Moreover, the ß-CD extract induced Akt activation and completely abolished the TNFα-induced p38MAPK phosphorylation. UAE and ß-CD encapsulation provide an efficient extraction protocol that increases polyphenol bioavailability. Polyphenols from MI play a protective role on endothelial cells and may be further considered as oestrogen-like molecules with vascular protective properties.
Subject(s)
Endothelial Cells/drug effects , Mangifera/chemistry , Plant Bark/chemistry , Plant Extracts/chemistry , Polyphenols/chemistry , Cell Line , Cyclooxygenase 2/metabolism , Humans , Intercellular Adhesion Molecule-1/metabolism , Interleukin-6/metabolism , Interleukin-8/metabolism , Matrix Metalloproteinase 2/metabolism , Matrix Metalloproteinase 9/metabolism , Nitric Oxide Synthase Type III/metabolism , Phosphorylation , Signal Transduction , Tumor Necrosis Factor-alpha/pharmacology , Ultrasonics , beta-Cyclodextrins , p38 Mitogen-Activated Protein Kinases/metabolismABSTRACT
The antiradical power of the methanol extracts of olive pomace (Taggiasca cultivar) achieved by high-pressure-high-temperature reactor were investigated using ABTSâ¢(+) and DPPH⢠assays. The highest antioxidant activity was quantified at 90 min of contact time and 180°C of extraction temperature (64.19 ± 0.16 µg(TE) L(-1) and 15.80 ± 0.62 µg(DPPH) µL(extract) (-1)). The extract with high-antioxidant power resulted to be effective to counteract key aspects of cellular oxidation sensitive mechanisms and inflammation associated to vascular diseases. A linear correlation (p < 0.05) between total polyphenol contents and antioxidant capacity was given by the ABTSâ¢(+) method (R (2) = 0.9184) and DPPH assay (R (2 )= 0.7062).