Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters

Database
Language
Affiliation country
Publication year range
1.
Int J Biol Macromol ; 102: 779-788, 2017 Sep.
Article in English | MEDLINE | ID: mdl-28412339

ABSTRACT

Microbial amylases are used to produce ethanol, glucose and can be applied in textiles products, detergents and other industries. This study aimed to determine the best carbon source concentration to induce the amylase production by A. japonicus, and its purification and biochemical characterization. For that, this fungus was cultivated in Khanna medium, pH 5.5, for 4 days, at 25°C, in static condition, supplemented with potato starch and maltose in different concentrations. The fungal crude enzymatic extract was purified in a unique elution in DEAE-cellulose column and the molecular mass was determined as 72kDa. The optimum temperature and pH was 65°C and 5.0, respectively. Amylase remained 75% of its activity after one hour at 50°C and was stable in the pH range 3.0-7.0. The analysis of the end-products by thin layer chromatography showed only glucose formation, which characterizes the purified enzyme as a glucoamylase. Amylopectin was the best substrate for the enzyme assay and Mn+2 and Pb+2 were good glucoamylase activators. This activation, in addition to the biochemical characteristics are important results for future biotechnological applications of this glucoamylase in the recycling and deinking process by the paper industries.


Subject(s)
Aspergillus/enzymology , Glucan 1,4-alpha-Glucosidase/isolation & purification , Glucan 1,4-alpha-Glucosidase/metabolism , Lead/pharmacology , Manganese/pharmacology , Amylose/metabolism , Dose-Response Relationship, Drug , Edetic Acid/pharmacology , Enzyme Activation/drug effects , Glucan 1,4-alpha-Glucosidase/chemistry , Hydrogen-Ion Concentration , Hydrolysis , Kinetics , Maltose/pharmacology , Mercaptoethanol/pharmacology , Molecular Weight , Phylogeny , Temperature
SELECTION OF CITATIONS
SEARCH DETAIL