Network Pharmacology and Molecular Docking Identify the Potential Mechanism and Therapeutic Role of Scutellaria baicalensis in Alzheimer's Disease.

Aim: Scutellaria baicalensis, a traditional Chinese medicinal herb renowned for its anti-inflammatory, antioxidant, and anti-tumor properties, has shown promise in alleviating cognitive impairment associated with Alzheimer's disease. Nonetheless, the exact neuroprotective mechanism of Scutellaria baicalensis against Alzheimer's disease remains unclear. In this study, network pharmacology was employed to explore the possible mechanisms by which Scutellaria baicalensis protects against Alzheimer's disease. Methods: The active compounds of Scutellaria baicalensis were retrieved from the TCMSP database, and their corresponding targets were identified. Alzheimer's disease-related targets were obtained through searches in the GeneCards and OMIM databases. Cytoscape 3.6.0 software was utilized to construct a regulatory network illustrating the "active ingredient-target" relationships. Subsequently, the target genes affected by Scutellaria baicalensis in the context of Alzheimer's disease were input into the String database to establish a PPI network. GO analysis and KEGG analysis were conducted using the DAVID database to predict the potential pathways associated with these key targets. Following this, the capacity of these active ingredients to bind to core targets was confirmed through molecular docking. In vitro experiments were then carried out for further validation. Results: A total of 36 active ingredients from Scutellaria baicalensis were screened out, which corresponded to 365 targets. Molecular docking results demonstrated the robust binding abilities of Baicalein, Wogonin, and 5,2'-Dihydroxy-6,7,8-trimethoxyflavone to key target proteins (SRC, PIK3R1, and STAT3). In vitro experiments showed that the active components of Scutellaria baicalensis can inhibit STAT3 expression by downregulating the PIK3R1/SRC pathway in Neuro 2A cells. Conclusion: In summary, these findings collectively suggest that Scutellaria baicalensis holds promise as a viable treatment option for Alzheimer's disease.

Amino acid application inhibits root-to-shoot cadmium translocation in Chinese cabbage by modulating pectin methyl-esterification.

The exogenous application of amino acids (AAs) generally alleviates cadmium (Cd) toxicity in plants by altering their subcellular distribution. However, the physiological mechanisms underlying AA-mediated cell wall (CW) sequestration of Cd in Chinese cabbage remain unclear. Using two genotypes of Chinses cabbage, Jingcui 60 (Cd-tolerant) and 16-7 (Cd-sensitive), we characterized the root structure, subcellular distribution of Cd, CW component, and related gene expression under the Cd stress. Cysteine (Cys) supplementation led to a reduction in the Cd concentration in the shoots of Jingcui 60 and 16-7 by 65.09 % and 64.03 %, respectively. Addition of Cys alleviated leaf chlorosis in both cultivars by increasing Cd chelation in the root CW and reducing its distribution in the cytoplasm and organelles. We further demonstrated that Cys supplementation mediated the downregulation of PMEI1 expression and improving the activity of pectin methyl-esterase (PME) by 17.98 % and 25.52 % in both cultivars, respectively, compared to the Cd treatment, resulting in an approximate 12.00 %-14.70 % increase in Cd retention in pectin. In contrast, threonine (Thr) application did not significantly alter Cd distribution in the shoots of either cultivar. Taken together, our results suggest that Cys application reduces Cd root-to-shoot translocation by increasing Cd sequestration in the root CW through the downregulation of pectin methyl-esterification.

Metal oxide modified biochars for fertile soil management: Effects on soil phosphorus transformation, enzyme activity, microbe community, and plant growth.

Metal oxide modified biochars are increasingly being used for intensive agricultural soil remediation, but there has been limited research on their effects on soil phosphorus transformation, soil enzyme activity, microbe community and plant growth. Two highly-performance metal oxides biochars (FeAl-biochar and MgAl-biochar) were investigated for their effects on soil phosphorus availability, fractions, enzyme activity, microbe community and plant growth in two typical intensive fertile agricultural soils. Adding raw biochar to acidic soil increased NH4Cl-P content, while metal oxide biochar reduced NH4Cl-P content by binding to phosphorus. Original biochar slightly reduced Al-P content in lateritic red soil, while metal oxide biochar increased it. LBC and FBC significantly reduced Ca2-P and Ca8-P properties while improving Al-P and Fe-P, respectively. Inorganic phosphorus-solubilizing bacteria increased in abundance with biochar amendment in both soil types, and biochar addition affected soil pH and phosphorus fractions, leading to changes in bacterial growth and community structure. Biochar's microporous structure allowed it to adsorb phosphorus and aluminum ions, making them more available for plants and reducing leaching. In calcareous soils, biochar additions may dominantly increase the Ca (hydro)oxides bounded P or soluble P instead of Fe-P or Al-P through biotic pathways, favoring plant growth. The recommendations for using metal oxides biochar for fertile soil management include using LBC biochar for optimal performance in both P leaching reduction and plant growth promotion, with the mechanisms differing depending on soil type. This research highlights the potential of metal oxide modified biochars for improving soil fertility and reducing phosphorus leaching, with specific recommendations for their use in different soil types.

Interception of fertile soil phosphorus leaching with immobilization materials: Recent progresses, opportunities and challenges.

The non-point source pollution induced by phosphorus (P) leaching from fertile soils is accelerating the eutrophication phenomena in aqueous ecosystems. Herein, to alleviate and intercept the P leaching from the fertile soils, diverse P immobilization materials (PIM) which can transform labile P into stable P via a range of physicochemical and biological interactions have been adopted and received increasing research interest. However, the remediation mechanisms of different PIMs were complex and vary with soil properties and PIM application methods. In this review, the P fraction and mobility characteristics of different fertile soils were first introduced. Then, three kinds of PIM including inorganic materials (e.g., clay minerals and red mud), organic materials (e.g., polyacrylamide), and composites (e.g., modified biochar) applied in soil P leaching interception were concluded. The key factors (i.e., soil pH, soil texture, organic matter content and variable soil moisture) influencing PIM performance and potential PIMs used for reducing soil P leaching were also introduced. Current review can favor for proposing more suitable and insightful strategies to regulate the fertile soil P and achieve the dual goals of improving the crop land quality and yield, and preventing agricultural non-point source pollution.

Identifying biotic and abiotic processes of reversing biochar-induced soil phosphorus leaching through biochar modification with MgAl layered (hydr)oxides.

Biochar (BC) as a increasing widely adopted soil amendments showed potential threat to soil P leaching, but the relevant mechanisms were not clear enough and relevant strategy should be proposed to address the P leaching induced by BC application. In this study, effects of ordinary corn straw BC, and a fabricated Mg/Al-LDHs modified biochar (LBC) on soil P availability, adsorption, fraction and mobility were compared and investigated by conducting the column and incubation experiments at biochar to soil rate of 1 %, 2 % and 4 % (w/w). Chemical sequential extraction methods and various solid-state method (i.e., three-dimensional excitation emission matrix (EEM), x-ray diffraction (XRD), scanning electron micrograph (SEM) and P K-edge X-ray absorption near edge structure (XANES)) were utilized to give deep insights into the P mobilization and immobilization mechanisms by respectively applying the BC and LBC. Results of incubation experiments showed that applying the LBC reduced the labile P with significant CaP transformation to Al-retained P, while ordinary BC promoted the Fe/Al-P transformation to labile dibasic calcium phosphate and monobasic calcium phosphate evidenced by the EEM analysis, in-situ XANES investigation and chemical sequential extraction methods. Results of phosphatase and microbial analyses indicated that the decreased labile P after 30 days' incubation and the mitigated P leaching in LBC treatment were dominantly ascribed to abiotic processes of inorganic P transformation and (de)sorption. This research gave deep insights into abiotic and biotic processes of ordinary biochar promoting soil P leaching, and important implications for applying engineered biochar in reducing P leaching and improving soil productivity.

Effects of phosphorus-containing material application on soil cadmium bioavailability: a meta-analysis.

Diverse phosphorus-containing materials (PCMs) were widely applied in remediation of cadmium-contaminated soils, and their effects on the change of soil cadmium availability (SCA) varied with their physicochemical characteristics and environmental conditions. Investigation on the effect of various PCMs on reducing SCA under different conditions favors the safe utilization of Cd-contaminated soil. Herein, a meta-analysis of literature published before August 2021 was carried out. A total of 342 independent observations were obtained from 42 published papers which included 9 factors that may affect the passivation effect of fertilizer content: phosphorus type, phosphorus application rate, soil pH, soil CEC, soil organic matter, experiment type, and time. Results of boosted regression tree analysis showed that the application rate is the most important factor contributing to the SCA, followed by soil pH and duration. Results of this meta-analysis showed that medium P input shows potential for reactivating the SCA. Under alkaline soil conditions and high soil CEC values, PCM input can better deactivate SCA. In addition, the difference from the previous understanding is that under the medium input of phosphorus-containing fertilizer (90-500 mg P∙kg-1), it will significantly increase the content of available cadmium in soil. In addition, future recommendation for exploring novel PCMs and suitable strategies for controlling the SCA though PCM application were also proposed. Our works may promote the interpretation of the interference factors on the SCA changes and fill the research gaps on utilization of PCM in Cd-polluted soil remediation.

Activated low-grade phosphate rocks for simultaneously reducing the phosphorus loss and cadmium uptake by rice in paddy soil.

Cadmium (Cd) pollution and phosphorus (P) leaching in paddy soils has raised the global concern. In this study, two kinds of the low grade phosphate rocks activated by the sodium lignosulfonate (SL) and humic acid (HA) were fabricated for soil Cd passivation and reduction of the soil P leaching simultaneously. The mechanisms of the Cd adsorption and passivation by the activated phosphate rocks (APRs) were investigated through the batch experiment and the indoor culture test (i.e., incubation and pot experiments) in the Cd-polluted paddy soil. The effects of the APRs on the potted rice growth, uptake of Cd by rice and P loss were also studied. In comparison with the superphosphate treatment, the cumulative P loss from SL- and HA-APRs were reduced by the 65.2% and 65.3%. In terms of the Cd passivation, the Cd adsorbed on the APRs was through the chemical ways (i.e., ligand exchange and the formation of internal complexes). The application of the APRs significantly decreased the soil exchangeable Cd by 48.9%-55.0%, while the Fe/Mn oxides-bound Cd and residual Cd increased significantly by 19.6%-20.3% and 50.7%-69.4%, respectively. Pot experiment also suggested that both the APRs treatments (SL- and HA-APRs) significantly diminished soil Cd accumulation in rice (by 72.7% and 62.8%) coupling with the significantly decreased P leaching. These results provide a sustainable way to explore a novel cost-effective, high-efficient and bi-functional mineral-based soil amendments for environmental remediation.

Differences in main processes to transform phosphorus influenced by ammonium nitrogen in flooded intensive agricultural and steppe soils.

Exogenous ammonium nitrogen (AN) fertilization/deposition and the subsequently induced soil acidification, might exacerbate soil phosphorus (P) transformation and mobility, whereas poor understanding in abiotic and biotic processes still existed between intensively cultivated soil with high proportion of legacy inorganic P (Pi) in comparison to natural steppe soil with high proportion of organic P (Po). Column batch flooded experiment using vegetable (VEG) and steppe (STE) soils was conducted to investigate the P transformation and mobility influenced by 60-day intermittent elution with an (1500 mg N kg-1) solution or an acid sulfate (107 mmol H+ kg-1) solution. The results showed that AN elution significantly decreased the contents of all Pi fractions and strengthened Pi leaching, whereas acid elution increased the contents of Al-Pi, Fe-Pi, and reductant-soluble Pi and reduced P leaching in VEG soil. In contrast, AN elution increased the contents of soluble-Po and Al-(Pi + Po) and decreased Ca-P and Fe-Pi, no effects on P leaching, whereas acid elution increased the contents of soluble-(Pi + Po), Al-(Pi + Po), Fe-(Pi + Po) and reduced P leaching in STE soil. Variances analysis showed that pH and microbial biomass carbon were the most important factors to predict the P composition of the VEG and the STE soil, respectively. This indicated that AN elution released the soil mineral-bound phosphate with ammonia oxidation coupled with Fe(III) reduction, besides the same chemical influences on Fe/Al oxides as acid elution in VEG soil; while predominantly affected biochemical/biological processes of soil P by changing microbial biomass and enzyme activities in STE soil.