ABSTRACT
Summary: The clinical usefulness of two commercial peach extracts for SPT (by Lofarma SpA and ALK-Abellò, respectively) was compared in a multicenter study carried out in Italy. Peach allergic patients were tested with the two extracts in parallel and underwent the detection of IgE specific for all three peach allergens currently available (Pru p1, Pru p3, and Pru p4, respectively). The two extracts were almost identical in terms of sensitivity and specificity, being able to detect virtually all patients sensitized to stable peach allergens (lipid transfer protein (LTP) and, presumably, peamaclein) but scoring negative in patients exclusively sensitive to labile allergens (either PR-10 and/or profilin). Thus, the two extracts represent an excellent tool to carry out a preliminary component-resolved diagnosis of peach allergy at the first patient visit.
Subject(s)
Allergens/immunology , Antigens, Plant/immunology , Food Hypersensitivity/diagnosis , Plant Extracts , Plant Proteins/immunology , Prunus persica , Skin Tests/methods , Antigens, Plant/analysis , Carrier Proteins , Food Hypersensitivity/immunology , Humans , Immunoglobulin E , Plant Extracts/chemistry , Plant Extracts/immunology , Plant Proteins/analysisABSTRACT
BACKGROUND AND OBJECTIVES: Peach gibberellin-regulated protein (peamaclein) has recently emerged as a relevant food allergen in cypress pollen-hypersensitive patients. Objective: We investigated monosensitization to peamaclein among Italian cypress pollen-allergic patients. MATERIAL AND METHODS: A total of 835 cypress pollen-hypersensitive patients from 28 Italian allergy centers underwent a thorough work-up to determine food-allergic reactions and performed skin prick testing with a commercial peach extract containing peamaclein. IgE to rPru p 3 was measured in peach reactors, and those with negative results were enrolled as potentially monosensitized to peamaclein. IgE reactivity to rPru p 7 was evaluated using immunoblot and an experimental ImmunoCAP with rPru p 7. RESULTS: Skin prick tests were positive to peach in 163 patients (19.5%); however, 127 (77.9%) were excluded because they reacted to Pru p 3. Twenty-four patients (14.7%) corresponding to 2.8% of the entire study population) were considered potentially monosensitized to peamaclein. No geographic preference was observed. Seventeen of the 24 patients (70.8%) had a history of food allergy, mainly to peach (n=15). Additional offending foods included other Rosaceae, citrus fruits, fig, melon, tree nuts, and kiwi. On peach immunoblot, only 3 of 18 putative peamaclein-allergic patients reacted to a band at about 7 kDa; an additional 4 patients reacted at about 50-60 kDa. Ten of 18 patients (56%) had a positive result for Pru p 7 on ImmunoCAP. CONCLUSION: Allergy and sensitization to peamaclein seem rare in Italy. Most patients react to peach, although other Rosaceae fruits and several citrus fruits may also be offending foods. Peach and cypress pollen probably also share cross-reacting allergens other than peamaclein.
Subject(s)
Cupressus , Food Hypersensitivity , Allergens/adverse effects , Antigens, Plant/adverse effects , Cross Reactions , Food Hypersensitivity/epidemiology , Gibberellins , Humans , Immunoglobulin E , Plant Proteins/adverse effects , Pollen , Skin Tests/adverse effectsSubject(s)
Antigens, Plant/metabolism , Clinical Decision-Making/methods , Particulate Matter/metabolism , Pollen/metabolism , Rhinitis, Allergic, Seasonal/diagnosis , Adult , Allergens/immunology , Antigens, Plant/immunology , Fagales , Female , Humans , Male , Mediterranean Region/epidemiology , Particulate Matter/immunology , Pinales , Poaceae , Pollen/immunology , Rhinitis, Allergic, Seasonal/epidemiology , SeasonsABSTRACT
Arthritic diseases are the most frequent causes of chronic pain and disability. Rheumatoid arthritis (RA) is an autoimmune disease characterized by synovial inflammation and progressive structural joint damage. Osteoarthritis is a degenerative process of the articular cartilage associated with hypertrophic changes in the bone. The aim of the present study was to investigate the anti-inflammatory and analgesic effects of Hévíz thermal water and mud in monosodium iodoacetate- (MIA-) (25 mg/ml, 20 µl i.a.) induced osteoarthritis and Complete Freund's adjuvant- (CFA-) (1 mg/ml, 50-50 µl s.c) induced rheumatoid arthritis murine models. The mechanonociceptive threshold of female NMRI mice (n=6- 8 mice/ group) was measured by aesthesiometry, and paw volume was monitored with plethysmometry, knee joint diameter with digital micrometer, and dynamic weight bearing on the hind limbs with a Bioseb instrument. Periarticular bone destruction was assessed by SkyScan 1176 in vivo micro-CT. Inflammatory cytokines were detected by ELISA in plasma samples. Treatments (30 min, every working day) with tap water, sand, and a combined therapy of tap water and sand served as controls. Hévíz medicinal water and combined treatment with water and mud significantly decreased the mechanical hyperalgesia and knee oedema in MIA-induced osteoarthritis model. However, balneotherapy did not influence mechanical hyperalgesia, weight bearing, or oedema formation induced by CFA. Neither medicinal water nor mud treatment ameliorated deep structural damage of the bones or the joints in the animal models. On the basis of the present findings, we conclude that balneotherapy is an effective complementary treatment to reduce the pain sensation and swelling in degenerative joint diseases such as osteoarthritis. Our experimental data are in agreement with the previous human studies that also confirmed antinociceptive and anti-inflammatory effects of thermal water and Hévíz mud treatments.
ABSTRACT
Hydrogen sulfide (H2S) is a toxic gas. It has been recognized that H2S evolving in biochemical reactions in living organisms has an important role in different physiologic processes. Nowadays, H2S is known as an endogenous messenger molecule. Natural sulfurous spring water has been proved beneficial in the therapy of diseases of the skin and other organs (Boros et al 2013). In vivo real-time detection of local H2S concentration is an important but challenging task.We developed a two-electrode amperometric cell for selective subcutaneous detection of H2S in anesthetized mice. The cell is a small size implantable gas sensor containing a platinum disc anode and a silver cathode. The selectivity is provided by a membrane permeable only by gases. There is a buffered reversible electrochemical mediator solution in an oxidized form inside the cell. As gaseous H2S penetrates into the cell the mediator is reduced, and +0.4 V versus the reference is employed on the platinum working electrode. The reduced mediator is oxidized on the anode surface. The current provides an analytical signal representing the concentration of H2S.Appropriate shape, size and membrane material were selected, and optimal working parameters--such as mediator concentration, pH and cell voltage--were determined in vitro. The lower limit of detection in the stirred sample solution at pH = 5.5 was as small as 9.4 × 10(-7) M and a dynamic concentration range of 0-6 × 10(-4) M could be achieved.The detecting surfaces of the cell were covered with freshly dissected mouse skin to test dermal H2S permeability. In other experiments, the cell was implanted subcutaneously in an anesthetized mouse and the animal was submerged in a buffer solution containing different concentrations of H2S so that the skin surface over the sensor was covered by the solution. Measurements of subcutaneous H2S concentration were taken. The experiments clearly proved that H2S diffuses through the skin of the live mouse.
Subject(s)
Electrochemistry/instrumentation , Hydrogen Sulfide/analysis , Subcutaneous Tissue/chemistry , Anesthesia , Animals , Balneology , Ferricyanides/chemistry , Hot Springs , Hydrogen Sulfide/metabolism , Hydrogen Sulfide/therapeutic use , Hydrogen-Ion Concentration , Membranes, Artificial , Mice , Mice, Inbred BALB C , Permeability , Skin AbsorptionABSTRACT
Substance P (SP) and calcitonin gene-related peptide (CGRP) released from capsaicin-sensitive sensory nerves induce local neurogenic inflammation in the innervated area. The aim of the present study was to investigate the effects of an endogenous opioid peptide, endomorphin-1, on sensory neuropeptide release in vitro and acute neurogenic and non-neurogenic inflammatory reactions in vivo. Electrical field stimulation (EFS; 40 V, 0.1 ms, 10 Hz, 120 s; 1200 impulses) was performed to evoke SP and CGRP release from peptidergic afferents of the isolated rat tracheae which was determined from the incubation medium with radioimmunoassay. Neurogenic inflammation in the skin of the acutely denervated rat hind paw was induced by topical application of 1% mustard oil and detected by Evans Blue leakage. Mustard oil-induced ear swelling of the mouse was determined with a micrometer during 3 h and myeloperoxidase activity as an indicator of granulocyte accumulation was measured with spectrophotometry at 6 h. EFS evoked about a twofold elevation in the release of both pro-inflammatory sensory neuropeptides. Endomorphin-1 (5 nM-2 microM) diminished the release of SP and CGRP in a concentration-dependent manner, the EC50 values were 39.45 nM and 10.84 nM, respectively. The maximal inhibitory action was about 80% in both cases. Administration of endomorphin-1 (1-100 microg/kg i.p.) dose-dependently inhibited mustard oil-evoked neurogenic plasma protein extravasation in the rat skin as determined by microg Evans Blue per g wet tissue. Repeated i.p. injections of the 10 microg/kg dose three times per day for 10 days did not induce desensitization in this model. Neurogenic swelling of the mouse ear was also dose-dependently diminished by 1-100 microg/kg i.p. endomorphin-1, but non-neurogenic neutrophil accumulation was not influenced. These results suggest that endomorphin-1 is able to inhibit the outflow of pro-inflammatory sensory neuropeptides. Based on this mechanism of action it is also able to effectively diminish neurogenic inflammatory responses in vivo.
Subject(s)
Calcitonin Gene-Related Peptide/metabolism , Neurogenic Inflammation/metabolism , Neurons, Afferent/metabolism , Oligopeptides/metabolism , Substance P/metabolism , Animals , Electric Stimulation , Male , Mice , Mice, Inbred BALB C , Mustard Plant/toxicity , Neurogenic Inflammation/chemically induced , Plant Oils/toxicity , Rats , Rats, Wistar , Skin/drug effectsABSTRACT
OBJECTIVE: The participation of sensory neurons and transient receptor potential vanilloid 1 (TRPV1) receptors in phorbol 12-myristate 13-acetate (PMA)-induced nerve-sensitizing effect was examined. MATERIALS AND METHODS: PMA dissolved in acetone and acetone were applied to the ears of TRPV1 receptor knockout and wild-type mice. Different groups of animals received ibuprofen, anti-interleukin-1 beta (IL-1beta) antibody, resiniferatoxin (RTX) or capsaicin pretreatment. Ear thickness, myeloperoxidase activity and IL-1beta content of the ears were determined. Histological evaluation was performed. RESULTS: PMA exerted potentiating action on contralateral acetone-induced ear oedema, which was inhibited by ibuprofen, topical capsaicin desensitization of the acetone-treated ear as well as by systemic RTX pretreatment. Neither the lack of TRPV1 receptors nor anti-IL-1beta antibody prevented sensitizing effect. CONCLUSIONS: The TRPV1 receptor-independent potentiating action of PMA on contralateral acetone-induced ear oedema is mediated via capsaicin-sensitive afferents and prostanoids are involved. IL-1beta is not essential in this process.
Subject(s)
Acetone/pharmacology , Ear/pathology , Edema/immunology , TRPV Cation Channels/physiology , Acetone/administration & dosage , Administration, Cutaneous , Afferent Pathways , Animals , Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Antibodies/pharmacology , Capsaicin/pharmacology , Diterpenes/pharmacology , Drug Synergism , Ear/innervation , Edema/chemically induced , Edema/pathology , Ibuprofen/pharmacology , Interleukin-1beta/immunology , Interleukin-1beta/metabolism , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Peroxidase/metabolism , Sensory Receptor Cells/physiopathology , TRPV Cation Channels/genetics , Tetradecanoylphorbol Acetate/administration & dosage , Tetradecanoylphorbol Acetate/pharmacologyABSTRACT
BACKGROUND AND PURPOSE: Substance P (SP) and calcitonin gene-related peptide (CGRP) released from capsaicin-sensitive sensory nerves induce local neurogenic inflammation; somatostatin exerts systemic anti-inflammatory actions presumably via sst4/sst1 receptors. This study investigates the effects of a high affinity, sst4-selective, synthetic agonist, J-2156, on sensory neuropeptide release in vitro and inflammatory processes in vivo. EXPERIMENTAL APPROACH: Electrically-induced SP, CGRP and somatostatin release from isolated rat tracheae was measured with radioimmunoassay. Mustard oil-induced neurogenic inflammation in rat hindpaw skin was determined by Evans blue leakage and in the mouse ear with micrometry. Dextran-, carrageenan- or bradykinin-induced non-neurogenic inflammation was examined with plethysmometry or Evans blue, respectively. Adjuvant-induced chronic arthritis was assessed by plethysmometry and histological scoring. Granulocyte accumulation was determined with myeloperoxidase assay and IL-1beta with ELISA. KEY RESULTS: J-2156 (10-2000 nM) diminished electrically-evoked neuropeptide release in a concentration-dependent manner. EC50 for the inhibition of substance P, CGRP and somatostatin release were 11.6 nM, 14.3 nM and 110.7 nM, respectively. J-2156 (1-100 microg kg(-1) i.p.) significantly, but not dose-dependently, inhibited neurogenic and non-neurogenic acute inflammatory processes and adjuvant-induced chronic oedema and arthritic changes. Endotoxin-evoked myeloperoxidase activity and IL-1beta production in the lung, but not IL-1beta- or zymosan-induced leukocyte accumulation in the skin were significantly diminished by J-2156. CONCLUSIONS AND IMPLICATIONS: J-2156 acting on sst4 receptors inhibits neuropeptide release, vascular components of acute inflammatory processes, endotoxin-induced granulocyte accumulation and IL-1beta synthesis in the lung and synovial and inflammatory cells in chronic arthritis. Therefore it might be a promising lead for the development of novel anti-inflammatory drugs.
Subject(s)
Anti-Inflammatory Agents/pharmacology , Butanes/pharmacology , Inflammation/prevention & control , Membrane Proteins/agonists , Naphthalenes/pharmacology , Neuropeptides/metabolism , Receptors, Somatostatin/agonists , Sulfones/pharmacology , Trachea/drug effects , Animals , Anti-Inflammatory Agents/therapeutic use , Arthritis, Experimental/prevention & control , Butanes/therapeutic use , Carrageenan , Dose-Response Relationship, Drug , Edema/chemically induced , Edema/prevention & control , Electric Stimulation , Inflammation/chemically induced , Inflammation/metabolism , Lipopolysaccharides , Male , Membrane Proteins/metabolism , Mice , Mice, Inbred BALB C , Mustard Plant , Naphthalenes/therapeutic use , Neurogenic Inflammation/prevention & control , Plant Oils , Pulmonary Eosinophilia/chemically induced , Pulmonary Eosinophilia/prevention & control , Rats , Rats, Inbred Lew , Rats, Wistar , Receptors, Somatostatin/metabolism , Sulfones/therapeutic use , Trachea/metabolismABSTRACT
Substance P (SP) and calcitonin gene-related peptide (CGRP), released from capsaicin-sensitive sensory nerves induce local neurogenic inflammation, while somatostatin exerts systemic anti-inflammatory actions. The aim of the present study was to investigate the release of pituitary adenylate cyclase activating polypeptide-38 (PACAP-38) and its effects on sensory neuropeptide release in vitro and acute neurogenic ear swelling in vivo. Capsaicin (10(-6) M) or electrical field stimulation (EFS; 40 V, 0.1 ms, 10 Hz, 120 s; 1200 impulses)-induced release of PACAP-38, SP, CGRP and somatostatin from isolated rat tracheae was measured with radioimmunoassay. Mustard oil-induced neurogenic inflammation in the mouse ear was determined with a micrometer and in the rat hind paw skin by the Evans Blue leakage technique. Capsaicin and EFS evoked 27% and more than twofold elevation of PACAP-38 release respectively, compared with the prestimulated basal values from isolated trachea preparation. Exogenously administered PACAP-38 (20-2000 nM) diminished both capsaicin- and EFS-evoked sensory neuropeptide release in a concentration-dependent manner. The maximal inhibitory effects of PACAP on capsaicin-induced substance P, CGRP and somatostatin release amounted to 75.4%, 73.3% and 90.0%, while EFS-evoked release of these peptides was 80.03%, 87.7% and 67.7%. In case of capsaicin stimulation the EC50 values for substance P, CGRP and somatostatin were 82.9 nM, 60.1 nM and 66.9 nM, respectively. When EFS was performed, these corresponding EC50 data were 92.1 nM, 67.8 nM and 20.9 nM. PACAP-38 (10, 100 and 1000 microg/kg i.p. in 200 microl volume) inhibited neurogenic ear swelling in the mouse. Furthermore, 100 microg/kg i.p. PACAP also significantly diminished mustard oil-evoked plasma protein extravasation in the rat skin. These results suggest that PACAP-38 is released from the stimulated peripheral terminals of capsaicin-sensitive afferents and it is able to inhibit the outflow of sensory neuropeptides. Based on this mechanism of action PACAP is also able to effectively diminish/abolish neurogenic inflammatory response in vivo after systemic administration.
Subject(s)
Calcitonin Gene-Related Peptide/metabolism , Neurogenic Inflammation/metabolism , Pituitary Adenylate Cyclase-Activating Polypeptide/metabolism , Substance P/metabolism , Animals , Capsaicin/pharmacology , Dose-Response Relationship, Drug , Dose-Response Relationship, Radiation , Drug Interactions , Ear/innervation , Ear/pathology , Electric Stimulation/methods , Hindlimb/innervation , Hindlimb/pathology , In Vitro Techniques , Male , Mice , Mice, Inbred BALB C , Mustard Plant , Neurogenic Inflammation/chemically induced , Neurogenic Inflammation/drug therapy , Neurogenic Inflammation/pathology , Pituitary Adenylate Cyclase-Activating Polypeptide/pharmacology , Plant Oils , Radioimmunoassay/methods , Rats , Rats, Wistar , Somatostatin/metabolismABSTRACT
A neurogenic component has been suggested to play a pivotal role in a range of inflammatory/immune diseases. Mustard oil (allyl-isothiocyanate) has been used in studies of inflammation to mediate neurogenic vasodilatation and oedema in rodent skin. The aim of the present study was to analyse mustard oil-induced oedema and neutrophil accumulation in the mouse ear focussing on the roles of neurokinin 1 (NK(1)) and vanilloid (TRPV1) receptors using normal (BALB/c, C57BL/6) as well as NK(1) and TRPV1 receptor knockout mice. A single or double treatment of 1% mustard oil on the BALB/c mouse ear induced ear oedema with responses diminished by 6 h. However a 25-30% increase in ear thickness was maintained by the hourly reapplication of mustard oil. Desensitisation of sensory nerves with capsaicin, or the NK(1) receptor antagonist SR140333, inhibited oedema but only in the first 3 h. Neutrophil accumulation in response to mustard oil was inhibited neither by SR140333 nor capsaicin pre-treatment. An activating dose of capsaicin (2.5%) induced a large oedema in C57BL/6 wild-type mice that was minimal in TRPV1 receptor knockout mice. By comparison, mustard oil generated ear swelling was inhibited by SR140333 in wild-type and TRPV1 knockout mice. Repeated administration of mustard oil maintained 35% oedema in TRPV1 knockout animals and the lack of TRPV1 receptors did not alter the leukocyte accumulation. In contrast repeated treatment caused about 20% ear oedema in Sv129+C57BL/6 wild-type mice but the absence of NK(1) receptors significantly decreased the response. Neutrophil accumulation showed similar values in both groups. This study has revealed that mustard oil can act via both neurogenic and non-neurogenic mechanisms to mediate inflammation in the mouse ear. Importantly, the activation of the sensory nerves was still observed in TRPV1 knockout mice indicating that the neurogenic inflammatory component occurs via a TRPV1 receptor independent process.
Subject(s)
Inflammation/classification , Plant Extracts/toxicity , Receptors, Drug/metabolism , Receptors, Neurokinin-1/metabolism , Animals , Capillary Permeability/drug effects , Capsaicin/pharmacology , Dose-Response Relationship, Drug , Ear/innervation , Edema/chemically induced , Inflammation/chemically induced , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Knockout , Mustard Plant , Neurokinin-1 Receptor Antagonists , Piperidines/pharmacology , Plant Oils , Quinuclidines/pharmacology , Receptors, Drug/genetics , Receptors, Neurokinin-1/genetics , Staining and Labeling/methods , TRPV Cation Channels , Time FactorsABSTRACT
1. Somatostatin (6.11 nmol kg(-1) i.p.) inhibited neurogenic plasma extravasation evoked by 1% mustard oil and non-neurogenic oedema induced by 5% dextran in the rat skin. 2. Cyclic synthetic octapeptide (TT-248 and TT-250) and heptapeptide (TT-232) somatostatin analogues proved to be more effective in reducing neurogenic and non-neurogenic inflammatory reactions but octreotide had no influence on either neurogenic or non-neurogenic inflammation. 3. TT-232 administered i.p. or i.v. (1.06 - 42.40 nmol kg(-1)) inhibited in a dose-dependent manner the plasma extravasation evoked by mustard oil in the rat's paw. Neither diclofenac (15.78 - 315.60 micromol kg(-1)) nor the selective COX-2 inhibitor meloxicam (2.95 - 569.38 micromol kg(-1)) attenuated the mustard oil-induced neurogenic plasma extravasation. 4. TT-232, diclofenac and meloxicam dose-dependently diminished non-neurogenic dextran-oedema of the paw the ED(35) values were 1.73 nmol kg(-1) for TT-232 and 34.37 micromol kg(-1) for diclofenac. 5. TT-232 inhibited in the dose range of 1.06 - 21.21 nmol kg(-1) the bradykinin-induced plasma extravasation in the skin of the chronically denervated paw. 6. Mustard oil-induced cutaneous plasma extravasation was dose-dependently diminished by s.c. TT-232 1, 2, 4, 6 or 16 h after the treatment. TT-232 (2 x 106, 2 x 212 and 2 x 530 nmol kg(-1) per day s.c. for 18 days) caused dose-dependent inhibition of chronic Freund adjuvant-induced arthritis during the experimental period. 7. TT-232 (200 and 500 nM) inhibited the release of SP, CGRP and somatostatin from the rat isolated trachea induced by electrical field stimulation (40 V, 0.1 ms, 10 Hz, 120 s) or by capsaicin (10(-7) M), but did not influence the basal, non-stimulated peptide release. 8. It is concluded that somatostatin analogues without endocrine functions as TT-232 are promising compounds with a novel site of action for inhibition of non-neurogenic and neurogenic inflammatory processes.
Subject(s)
Anti-Inflammatory Agents/pharmacology , Somatostatin/pharmacology , Animals , Arthritis, Experimental/pathology , Arthritis, Experimental/prevention & control , Bradykinin/administration & dosage , Capillary Permeability/drug effects , Dextrans/administration & dosage , Diclofenac/pharmacology , Dose-Response Relationship, Drug , Evans Blue/metabolism , Female , Hindlimb/drug effects , Hindlimb/innervation , Hindlimb/pathology , In Vitro Techniques , Inflammation/chemically induced , Inflammation/prevention & control , Injections, Intraperitoneal , Meloxicam , Neuropeptides/drug effects , Neuropeptides/metabolism , Peptides, Cyclic/pharmacology , Rats , Rats, Wistar , Somatostatin/analogs & derivatives , Thiazines/pharmacology , Thiazoles/pharmacology , Time Factors , Trachea/drug effects , Trachea/metabolism , Treatment OutcomeABSTRACT
The systemic anti-inflammatory effect induced by antidromic sensory nerve stimulation was investigated in rats and guinea-pigs. In atropine-pretreated rats, bilateral antidromic stimulation of vagal afferent fibres (8 Hz, 20 min, at C-fibre strength) inhibited plasma extravasation induced by 1% mustard oil on the acutely denervated hindlegs by 36.45+/-3.95%. Both the prevention of this inhibitory effect by cysteamine pretreatment and the stimulation-evoked rise of plasma somatostatin-like immunoreactivity in the two species suggest a mediator role of neural somatostatin. Since this response was blocked by systemic capsaicin pretreatment and slightly reduced after subdiaphragmal vagotomy, participation of thoracic capsaicin-sensitive afferents is indicated. In guinea-pigs pretreated with guanethidine and pipecuronium, antidromic sciatic nerve stimulation induced 45.46+/-5.08% inhibition on the contralateral leg and increased plasma somatostatin-like immunoreactivity. It is concluded that somatostatin released from the activated vagal capsaicin-sensitive sensory nerve terminals of the rat and somatic nerves of the guinea-pigs exerts a systemic humoral function.
Subject(s)
Anti-Inflammatory Agents/blood , Capsaicin/pharmacology , Nerve Fibers/drug effects , Sciatic Nerve/drug effects , Somatostatin/blood , Vagus Nerve/drug effects , Afferent Pathways , Animals , Anti-Inflammatory Agents/immunology , Blood Pressure/drug effects , Capillary Permeability/drug effects , Electric Stimulation , Extravasation of Diagnostic and Therapeutic Materials , Female , Guanethidine/pharmacology , Guinea Pigs , Heart Rate/drug effects , Hindlimb , Inflammation/chemically induced , Inflammation/physiopathology , Mustard Plant , Pipecuronium/pharmacology , Plant Extracts/adverse effects , Plant Oils , Rats , Rats, Wistar , Sciatic Nerve/metabolism , Skin/blood supply , Skin/innervation , Skin/pathology , Somatostatin/immunology , Vagus Nerve/metabolismABSTRACT
In rats anaesthetized with urethan and pretreated with pipecuronium bromide nocifensive reaction of blood pressure elevation evoked by intraarterial capsaicin injection was inhibited over 40 min by bilateral antidromic stimulation of the sensory fibres of the sciatic nerves. Rise in blood pressure, heart rate and respiratory frequency evoked by capsaicin were markedly diminished after smearing 1% mustard oil on the acutely denervated hindpaws indicating a release of mediators with anti-nociceptive action from cutaneous nociceptors. Intravenous injection of the putative mediator somatostatin (10 microg/kg) or its analogues RC-160 and TT-232, but not octreotide inhibited the cardiorespiratory and blood pressure responses evoked by topical cutaneous application of mustard oil or capsaicin instillation into the eye. It is concluded, that the endocrine and the anti-nociceptive effects of somatostatin are mediated through distinct receptor subtypes and therefore, TT-232, a novel heptapeptide analogue without endocrine action, is a promising analgesic compound.
Subject(s)
Analgesics/pharmacology , Nerve Endings/metabolism , Neurons, Afferent/metabolism , Nociceptors/physiology , Peptides, Cyclic/pharmacology , Somatostatin/analogs & derivatives , Somatostatin/physiology , Animals , Blood Pressure/drug effects , Capsaicin/administration & dosage , Capsaicin/pharmacology , Female , Heart Rate/drug effects , Mustard Plant , Neurons, Afferent/physiology , Plant Extracts/administration & dosage , Plant Extracts/pharmacology , Plant Oils , Rats , Rats, Wistar , Respiration/drug effects , Skin/innervation , Somatostatin/metabolism , Somatostatin/pharmacologyABSTRACT
The effect of the non-peptide selective tachykinin NK1 receptor antagonist SR140333 has been investigated on oedema formation and neutrophil accumulation induced by thermal injury (50 degrees C for 5 min), mustard oil, substance P, the tachykinin NK1 agonist GR73632, and interleukin-1beta in the abdominal skin of the anaesthetised rat. SR140333 significantly inhibited (120 nmol/kg i.v.) or prevented (240 nmol/kg i.v.) the early oedema formation (0-10 min) induced by thermal injury. However, a dosing strategy which blocked NK1 receptors for 5 h (SR140333, 240 nmol/kg i.v. + 240 nmol/kg s.c.) failed to influence neutrophil accumulation measured 5 h after thermal injury. Thus, the neurogenic component mediated by NK1 receptors is important to elicit the early oedema formation, but does not influence subsequent neutrophil accumulation. Topical application of mustard oil (2%), a neurogenic inflammation stimulant, caused NK1 receptor-mediated early neurogenic plasma extravasation, but did not induce cutaneous neutrophil accumulation over 5 h. Substance P and GR73632 at high doses (1 nmol/site) also failed to elicit neutrophil accumulation. Neutrophil accumulation induced by interleukin-1beta (0.03-3 pmol i.d.) was not affected by SR140333 pretreatment. In conclusion, despite an early pronounced tachykinin NK1 receptor-dependent oedema response after thermal injury, the results suggest that subsequent neutrophil accumulation is not mediated by NK1 receptors. Furthermore, we have not obtained any evidence to suggest that either endogenous or exogenous tachykinins can directly induce neutrophil accumulation in the rat cutaneous microvasculature.
Subject(s)
Burns/physiopathology , Neutrophils/physiology , Receptors, Neurokinin-1/physiology , Skin/blood supply , Abdomen/innervation , Animals , Bradykinin/pharmacology , Burns/complications , Dose-Response Relationship, Drug , Edema/drug therapy , Edema/etiology , Edema/physiopathology , Hindlimb/innervation , Histamine/pharmacology , Inflammation/chemically induced , Inflammation/drug therapy , Interleukin-1/pharmacology , Male , Microcirculation/drug effects , Mustard Plant , Neurokinin-1 Receptor Antagonists , Neutrophils/cytology , Neutrophils/drug effects , Peptide Fragments/pharmacology , Piperidines/pharmacology , Plant Extracts/pharmacology , Plant Oils , Quinuclidines/pharmacology , Rats , Rats, Wistar , Receptors, Neurokinin-1/agonists , Skin/drug effects , Skin/pathology , Substance P/analogs & derivatives , Substance P/pharmacology , Time FactorsABSTRACT
A specific and sensitive radioimmunoassay was developed in our laboratory for measuring plasma and tissue somatostatin levels. The hormone content of arterial blood and skin samples of untreated and mustard oil (a specific agent causing neurogenic inflammation) treated animals was detected by this method. The somatostatin level of the inflamed tissue was significantly higher, but no difference was found between the plasma concentrations.
Subject(s)
Blood Chemical Analysis/methods , Radioimmunoassay/methods , Skin/chemistry , Somatostatin/analysis , Somatostatin/blood , Animals , Dermatitis/etiology , Dermatitis/metabolism , Mustard Plant , Nervous System Diseases/chemically induced , Nervous System Diseases/complications , Plant Extracts/pharmacology , Plant Oils , Rats , Rats, Wistar , Sensitivity and Specificity , Skin/drug effectsSubject(s)
Analgesics/pharmacology , Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Inflammation/physiopathology , Peptides, Cyclic/pharmacology , Somatostatin/analogs & derivatives , Somatostatin/pharmacology , Animals , Blood Pressure/drug effects , Dextrans , Edema , Heart Rate/drug effects , Hindlimb , Inflammation/drug therapy , Mustard Plant , Octreotide/pharmacology , Plant Extracts , Plant Oils , RatsSubject(s)
Evoked Potentials , Inflammation/physiopathology , Naloxone/pharmacology , Nerve Fibers/physiology , Neurons, Afferent/physiology , Skin/innervation , Animals , Edema , Enkephalins/physiology , Mustard Plant , Neuropeptides/physiology , Neurotransmitter Agents/physiology , Paraffin , Plant Extracts , Plant Oils , Plants, Medicinal , RatsABSTRACT
OBJECTIVE: The purpose of this article is to examine the role of yolk sac failure during organogenesis in the development of diabetes-associated embryopathy. METHODS: The current literature regarding congenital malformations in diabetic pregnancies was reviewed to elucidate the precise role of the yolk sac in embryonic development and the relation between yolk sac injury and embryopathy. RESULTS: We and others have demonstrated that hyperglycemia produces a teratogenic effect during organogenesis. In addition, we have shown that the yolk sac appears to be the target site of injury induced by hyperglycemia. We have also presented evidence that cell membrane dysfunction leads to failed vitelline vessel formation and that arachidonic acid supplementation prevents many of the morphologic and biochemical alterations observed under hyperglycemic conditions. CONCLUSIONS: These data strongly support the teratogenic effect of hyperglycemia, the arachidonic acid deficiency state, the resultant maldevelopment of vitelline vessels, and the ability to prevent these changes by arachidonic acid supplementation. These studies have made significant inroads in explaining why diabetes-associated anomalies occur, and suggest a potential future role for prophylaxis against these organogenetic malformations using dietary polyunsaturated fatty acid supplementation.
Subject(s)
Congenital Abnormalities/etiology , Diabetes Mellitus/pathology , Yolk Sac/pathology , Animals , Congenital Abnormalities/physiopathology , Diabetes Complications , Diabetes Mellitus/physiopathology , Disease Models, Animal , Embryonic and Fetal Development/physiology , Fatty Acids, Unsaturated/physiology , Hematopoiesis/physiology , Humans , Neovascularization, PathologicABSTRACT
Using the postimplantation rat conceptus model, we analyzed with gas-liquid chromatography, the fatty acid composition in major lipid groups (phospholipids, triglycerides, nonesterified fatty acids, and cholesterol esters) of yolk sacs and embryos cultured for 48 hours under control, hyperglycemic, and arachidonic acid-supplemented hyperglycemic conditions. In all experimental conditions the yolk sacs had greater fatty acid content than the embryos in all lipid groups except in nonesterified fatty acids. The fatty acid level in embryonic nonesterified fatty acids was significantly higher (p less than 0.05) in hyperglycemia-exposed embryos than found with arachidonic acid supplementation. Total yolk sac triglycerides were greater with added glucose (p less than 0.05) than with the addition of arachidonic acid to the same medium. Oleic acid, a fatty acid associated with essential fatty acid deficiency, was increased in the embryonic phospholipids and nonesterified fatty acids of conceptuses exposed to excess glucose, as well as in the culture media of this group, compared with the control or arachidonic acid-supplemented, hyperglycemic group (p less than 0.05). The results of this study demonstrate that diabetes-related embryopathy is associated with quantitative and qualitative abnormalities in major lipid groups. Furthermore, the elevation in embryonic oleic acid level suggests that the teratogenic mechanism could be related to a deficiency in essential fatty acids. The pattern of essential fatty acid deficiency and embryopathy was preventable with arachidonic acid supplementation in this experimental model.