Combined passivators regulate physiological, antioxidant potential and metals accumulation in potato grown in metals contaminated soil.

To solve the problem of excessive heavy metals in farmland soil, there is a dire need for research effort to screen for the soil passivator materials. This study aimed to develop a practical novel approach for improving the potato growth and remedial effectiveness of the metals by optimal combination and dosage of various passivators. Experimental treatments were comprised of various levels of passivating agents (sepiolite, quicklime and calcium magnesium phosphate) in individual and combined form. Results showed that application of passivating agents significantly enhanced growth by optimizing photosynthetic attributes, enzymatic antioxidants, and soil health. Balanced application of passivators effectively reduce the bioavailability of metals, curbing their uptake by potato plants. Sole application of all the agents results statistically similar outcomes as compared with combined form. Additionally, passivators indirectly enhance the activity of essential antioxidant enzymes. Synergistic effect of all the agents significantly improved the tuber quality by decreasing the accumulation of proline, malondialdehyde content, and bioaccumulation of Cu, Pb, Cd, and As in potato parts. In crux, combined usage of passivating agents proved to be of better growth, improvement in antioxidative defense system, and better quality of potato. By mitigating heavy metal contamination, passivators not only enhance crop quality and yield but also ensure heavy metal-free potatoes that meet stringent food safety standards.

Comparative efficacy of phosphorous supplements with phosphate solubilizing bacteria for optimizing wheat yield in calcareous soils.

Phosphorus (P) deficiency is the main hurdle in achieving sustainable crop production ps especially in calcareous soils. Using bio-fertilizers like phosphate solubilizing bacteria (PSB) could be a useful approach for sustainable P management as they improve P availability in soil via dissolution, desorption and mineralization reactions. In addition, application of organic amendments with PSB could further ameliorate soil conditions for sustainable management of immobilized nutrients in calcarious soils. Therefore, we performed pot experiment to study the role of PSB in nullifying antagonistic effects of liming (4.78, 10, 15 and 20%) on P availability from poultry manure (PM), farm yard manure (FYM), single super phosphate (SSP) and rock phosphate (RP) in alkaline soils. PSB inoculation improved wheat growth, P availability and stimulated soil acidification over control regardless of P sources and lime levels. Soil calcification adversely affected plant growth, P nutrition, induced soil salinity and alkalinity, however, PSB and manures application potentially nullified such harmful effects over mentioned traits. Individually, organic sources were superior than mineral sources however, the performance of mineral fertilizers with PSB was at par to sole application of manures. Furthermore, application of RP with PSB proved as effective as sole SSP. Therefore, using PSB as bio-fertilizer has huge potential for improving P availability in calcareous soils.

Relationship between the Chemical Composition and the Biological Functions of Coffee.

Coffee is a Rubiaceae coffee plant ranked as the first of the three most important beverages in the world, with effects including lowering blood sugar, protecting the liver, and protecting the nerves. Coffee contains many chemical components, including alkaloids, phenolic acids, flavonoids, terpenoids, and so on. Chemical components in coffee are the basis of its biological function and taste. The chemical components are the basis of biological activities and form the characteristic aroma of coffee. The main chemical components and biological activities of coffee have been extensively studied, which would provide a relevant basis and theoretical support for the further development of the coffee industry.

Antimicrobial, antioxidant and cytotoxic properties of Chenopodium glaucum L.

We evaluated phytochemical composition, antibacterial, antifungal, anti-oxidant and cytotoxic properties of aqueous (water) and organic extracts (methanol, ethyl acetate and n-hexane) of Chenopodium glaucum. Highest phenolic content 45 mg gallic acid equivalents (GAE)/g d.w was found in aqueous extract followed by ethyl acetate (41mg GAE/g d.w) and methanol extract (34.46 mg GAE/g d.w). Antibacterial potential of aqueous and organic extracts of C. glaucum was examined against Acinetobacter baumannii, Klebsiella pneumoniae, Escherichia coli and Staphylococcus epidermidis. The aqueous, methanolic, ethyl acetate, and n-hexane extract showed antibacterial activity against A. baumannii, K. pneumoniae, E. coli and S. epidermidis. However, against A. baumannii significantly higher inhibition zone (19 mm and 18.96 mm respectively) was shown by ethyl acetate and methanol extracts. Aqueous extract possessed highest growth inhibition (11 mm) against E. coli. Aqueous, ethyl acetate and methanol extracts showed 9 mm, 10 mm, and 10.33 mm zone of inhibition against the K. pneumoniae. For antifungal activity, the extracts were less effective against Aspergillus niger but showed strong antifungal activity against Aspergillus flavus (A. flavus). The antioxidant activity was measured as DPPH (2, 2-diphenyl-1-picrylhydrazyl), H2O2 and ABTS (2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) scavenging activity of free radicals. All the organic extracts of C. glaucum possessed ABTS, DPPH and H2O2 scavenging properties. The highest cytotoxic activity measured as half maximal inhibitory concentration (IC50) against human lungs carcinoma cells was recorded for methanolic (IC50 = 16 µg/mL) and n-hexane (IC50 = 25 µg/mL) extracts, respectively. The Gas chromatography-mass spectrometry (GC-MS) analysis showed 4 major and 26 minor compounds in n-hexane extract and 4 major and 7 minor compounds in methanol extract of the C. glaucum. It is concluded that aqueous and organic extracts of C. glaucum would be potential therapeutic agents and could be exploited on a pilot scale to treat human pathogenic diseases.

Coupling Phosphate-Solubilizing Bacteria with Phosphorus Supplements Improve Maize Phosphorus Acquisition and Growth under Lime Induced Salinity Stress.

Global warming promotes soil calcification and salinization processes. As a result, soil phosphorus (P) is becoming deficient in arid and semiarid areas throughout the world. In this pot study, we evaluated the potential of phosphate-solubilizing bacteria (PSB) for enhancing the growth and P uptake in maize under varying levels of lime (4.8%, 10%, 15% and 20%) and additional P supplements (farmyard manure, poultry manure, single super phosphate and rock phosphate) added at the rate of 45 mg P2O5 kg-1. Inoculation and application of P as organic manures (Poultry and farm yard manures) improved maize growth and P uptake compared to the control and soils with P applied from mineral sources. Liming adversely affected crop growth, but the use of PSB and organic manure significantly neutralized this harmful effect. Mineral P sources combined with PSB were as effective as the organic sources alone. Furthermore, while single supper phosphate showed better results than Rock phosphate, the latter performed comparably upon PSB inoculation. Thus, PSB plus P application as organic manures is an eco-friendly option to improve crop growth and P nutrition in a calcareous soil under changing climate.

Phosphate-Solubilizing Bacteria Nullify the Antagonistic Effect of Soil Calcification on Bioavailability of Phosphorus in Alkaline Soils.

Phosphate-solubilizing bacteria (PSB) reduce the negative effects of soil calcification on soil phosphorus (P) nutrition. In this incubation study, we explored the ability of PSB (control and inoculated) to release P from different P sources [single super phosphate (SSP), rock phosphate (RP), poultry manure (PM) and farm yard manure (FYM)] with various soil lime contents (4.78, 10, 15 and 20%) in alkaline soil. PSB inoculation progressively enriched Olsen extractable P from all sources compared to the control over the course of 56 days; however, this increase was greater from organic sources (PM and FYM) than from mineral P sources (SSP and RP). Lime addition to the soil decreased bioavailable P, but this effect was largely neutralized by PSB inoculation. PSB were the most viable in soil inoculated with PSB and amended with organic sources, while lime addition decreased PSB survival. Our findings imply that PSB inoculation can counteract the antagonistic effect of soil calcification on bioavailable P when it is applied using both mineral and organic sources, although organic sources support this process more efficiently than do mineral P sources. Therefore, PSB inoculation combined with organic manure application is one of the best options for improving soil P nutrition.

Optimizing the phosphorus use in cotton by using CSM-CROPGRO-cotton model for semi-arid climate of Vehari-Punjab, Pakistan.

Crop nutrient management is an essential component of any cropping system. With increasing concerns over environmental protection, improvement in fertilizer use efficiencies has become a prime goal in global agriculture system. Phosphorus (P) is one of the most important nutrients, and strategies are required to optimize its use in important arable crops like cotton (Gossypium hirsutum L.) that has great significance. Sustainable P use in crop production could significantly avoid environmental hazards resulting from over-P fertilization. Crop growth modeling has emerged as an effective tool to assess and predict the optimal nutrient requirements for different crops. In present study, Decision Support System for Agro-technology Transfer (DSSAT) sub-model CSM-CROPGRO-Cotton-P was evaluated to estimate the observed and simulated P use in two cotton cultivars grown at three P application rates under the semi-arid climate of southern Punjab, Pakistan. The results revealed that both the cultivars performed best at medium rate of P application (57 kg ha-1) in terms of days to anthesis, days to maturity, seed cotton yield, total dry matter production, and harvest index during 2013 and 2014. Cultivar FH-142 performed better than MNH-886 in terms of different yield components. There was a good agreement between observed and simulated days to anthesis (0 to 1 day), days to maturity (0 to 2 days), seed cotton yield, total dry matter, and harvest index with an error of -4.4 to 15%, 12-7.5%, and 13-9.5% in MNH-886 and for FH-142, 4-16%, 19-11%, and 16-8.3% for growing years 2013 and 2014, respectively. CROPGRO-Cotton-P would be a useful tool to forecast cotton yield under different levels of P in cotton production system of the semi-arid climate of Southern Punjab.

A combined application of biochar and phosphorus alleviates heat-induced adversities on physiological, agronomical and quality attributes of rice.

Present study examined the influence of high-temperature stress and different biochar and phosphorus (P) fertilization treatments on the growth, grain yield and quality of two rice cultivars (IR-64 and Huanghuazhan). Plants were subjected to high day temperature-HDT (35 °C ± 2), high night temperature-HNT (32 °C ± 2), and control temperature-CT (28 °C ± 2) in controlled growth chambers. The different fertilization treatments were control, biochar alone, phosphorous (P) alone and biochar + P. High-temperature stress severely reduced the photosynthesis, stomatal conductance, water use efficiency, and increased the leaf water potential of both rice cultivars. Grain yield and its related attributes except for number of panicles, were reduced under high temperature. The HDT posed more negative effects on rice physiological attributes, while HNT was more destructive for grain yield. High temperature stress also hampered the grain appearance and milling quality traits in both rice cultivars. The Huanghuazhan performed better than IR-64 under high-temperature stress with better growth and higher grain yield. Different soil fertilization treatments were helpful in ameliorating the detrimental effects of high temperature. Addition of biochar alone improved some growth and yield parameters but such positive effects were lower when compared with the combined application of biochar and P. The biochar+P application recorded 7% higher grain yield (plant(-1)) of rice compared with control averaged across different temperature treatments and cultivars. The highest grain production and better grain quality in biochar+P treatments might be due to enhanced photosynthesis, water use efficiency, and grain size, which compensated the adversities of high temperature stress.

A biochar application protects rice pollen from high-temperature stress.

The influences of high temperature and fertilization with biochar and phosphorus (P) on the pollen characteristics of two rice cultivars (IR-64 and Huanghuazhan) were examined in controlled growth chambers. Temperature treatments included high daytime temperature (HDT), high nighttime temperature (HNT) and ambient temperature (AT). The fertilization treatments were control, biochar alone, P alone and biochar + P. High temperature severely reduced pollen fertility, anther dehiscence, pollen retention and pollen germination of both rice cultivars, with HNT more destructive than HDT. The Huanghuazhan cultivar performed better than IR-64 under high temperature, with higher pollen fertility, better anther dehiscence and greater pollen retention and germination rates. In both cultivars, the pollen of plants treated with biochar + P were more resistant to heat induced stress. Further studies are needed to test the ability of biochar to ameliorate the effects of different abiotic stresses in rice and other crops.