ABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE: Launaea arborescens, its vernacular name is Mol-albina belonging to asteracaea family origin of the southwest of Algeria. This plant is used in folk medicines to treat gastroenteritis, diabetes, child aliment and other diseases; it is taken macerated or boiled. AIM: This study aims to evaluate the anti-inflammation an analgesic activity of the aqueous extract of Launaea arborescens (AqELA) and its pathway of action. METHODS: the investigation of anti-inflammatory and analgesic effects were done using formalin test, acetic acid test. For mechanism investigation, it was used hot plate test to induce opioid receptors, a histamine and serotonin test to induce edema paw, finally, for the TRPV1 receptor, it was used the capsaicin test. RESULTS: The aqueous extract of Launaea arborescens showed a significant inhibition of abdominal writhing test 95% and 100% inhibition of licking paw using acid acetic test and formalin test respectively (EC: 47 mg/kg and 104 mg/kg). The analgesic effect of the aqueous extract of Launaea arborescens showed inhibition of sensation of pain after 120 min compared to morphine effect. The aqueous extract of Launaea arborescens reduced paw volume after 180 min and 120 min for histamine and serotonin respectively with dose-dependent. Concerning of TRPV1 receptors, the inhibition was showed at doses 100 mg and 300 mg. CONCLUSION: Our results contribute towards validation of the traditional use of Launaea arborescens for inflammation ailment.
Subject(s)
Analgesics/pharmacology , Anti-Inflammatory Agents/pharmacology , Asteraceae/chemistry , Plant Extracts/pharmacology , Algeria , Analgesics/therapeutic use , Animals , Anti-Inflammatory Agents/therapeutic use , Behavior, Animal/drug effects , Capsaicin/toxicity , Edema/chemically induced , Edema/drug therapy , Formaldehyde/toxicity , Histamine/toxicity , Hot Temperature/adverse effects , Inflammation/drug therapy , Inflammation/etiology , Male , Medicine, Traditional , Mice, Inbred BALB C , Pain/drug therapy , Pain/etiology , Pain Measurement , Plant Extracts/therapeutic use , Serotonin/toxicity , Solutions/chemistry , TRPV Cation Channels/antagonists & inhibitorsABSTRACT
OBJECTIVE: To investigate the butanol fraction of the water/acetone extract and isolate of the new flavonoids from Launeae arboescens. METHODS: The compounds were isolated by liquid chromatographic methods and their structures were identified by using spectroscopic analysis. RESULTS: THE ISOLATED COMPOUNDS WERE IDENTIFIED AS: 7-O-[α-rhamnopyranosyl 4',5,6-Trihydroxy flavone 1,4',5'-Di-Methoxy 7-(5â³-Me Hexan)1-oyl flavanone 2, 3â³-isopropyl pyrano [1â³:7,4â³:6] 3',4',5',5-Tetrahydroxy flavanone 3,5,4',5'-Tri-Hydroxy 7-(3â³-Me butan) -yl flavanone 4, 5,7-Dihydroxy-2',4',5' -trimethoxy-isoflavanone 5,5,6,7,4'-tetrahydroxy flavonol 6,7-O-[α-rhamnopyranosyl-(1->6)-ß-glucopyranosyl]- 4',5,7-tri-hydroxy-flavanone 7,7-O-[α-rhamnopyranosyl-(1->6)-ß-glucopyranosyl] 3',5-Dihydroxy 4'-Methoxy flavanone 8. CONCLUSIONS: The presence of different types of bioactive flavonoids in Launeae arboescens extract can explain the large ethnopharmacological uses and the potential activity of this medicinal plant.
ABSTRACT
Urolithiasis can lead to the loss of renal function in some cases. In this study, we tested the inhibiting effect of wheat bran (Triticum aestivum L) extract on calcium oxalate crystallization in a turbidimetric model, by FTIR spectroscopy, and polarized microscopy. The results show that this plant extract has a major inhibitory effect on calcium oxalate crystallization.