ABSTRACT
<p><b>OBJECTIVE</b>To research the mechanism of the inhibition effects of BWE on cell attachment of influenza virus by capillary electrophoresis.</p><p><b>METHOD</b>The morphologic difference of red cells after treating with BWE infected by influenza virus was detected with microscope, capillary electrophoresis and HA.</p><p><b>RESULT</b>The pretreatment of the normal cells with BWE inhibited the attachment of influenza to the cells, while no meaningful inhibition was observed when influenza virus was pretreated before being inoculated to cells.</p><p><b>CONCLUSION</b>The results indicate that the inhibition effects of BWE on cell attachment of influenza virus may be an important mechanism of anti-influenza activity of Radix Isatidis Extracts.</p>
Subject(s)
Humans , Male , Antiviral Agents , Pharmacology , Drugs, Chinese Herbal , Pharmacology , Electrophoresis, Capillary , Erythrocytes , Virology , Hemagglutination Inhibition Tests , Influenza A virus , Isatis , Chemistry , Plant Roots , Chemistry , Plants, Medicinal , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To investigate the modulation of pilose antler extract (PAE) on rat osteogenic cells UMR-106 in vitro.</p><p><b>METHOD</b>Component P2 of PAE was isolated by Sephacryl S-200HR gel filtration chromatography. The proliferative effects of P2 and other components isolated by Sephacryl S-200HR on UMR-106 cells were investigated by MTT assay.</p><p><b>RESULT</b>The P2 could significantly increase the proliferation rate of osteogenic cells. When the protein concentration of P2 was between 0.972 mg x L(-1) and 97.2 mg x L(-1), it could inhibit the proliferation of UMR-106 cells. But while the concentration was equal to or greater than 97.2 mg x L(-1), the P2 could increase the proliferation rate of cells, especially 477.92% at 9.72 g x L(-1), which was approximated to 499.62% of PAE. The molecular weight of the P2 was about 59 kDa determined by SDS-PAGE. On the other hand, inhibition was also observed in the sample of the P3, P4 and P5.</p><p><b>CONCLUSION</b>Those regulative factors in PAE which have different molecular weight can affect the proliferation of UMR-106 cells two-wayly. And this adjustment also relies on the dose of those factors. This finding may help us to understand the possible mechanism of Chinese traditional medicine from animal materials.</p>