ABSTRACT
A comparative cytological analysis of intra- and intertissular cytomictic interactions in early micro-sporogenesis of mono- and dicotyledonous plants was performed by the example of the two cellular systems - microsporocytes and tapetum. It is found that cytomixis is the component of intratissular interactions mainly. In the tapetum cells cytomixis is notable for structural and temporary taxon specific features. The nuclear migration in microsporocytes is confined mainly to zygotene-pachytene meiotic stages and characterized by a certain synchronism with cytomixis at the tapetum. Intertissular cytomictic interactions (tapetum - microsporocytes) were found in the monocot anthers only. Intertissular interactions are likely to reflect the intensification of competitive relations between the tapetum and microsporocytes for area in the process of anther tissue differentiation. Polyploid tapetum nucleus and syncytia being powerful acceptors are able to compete with microsporocytes and direct the chromatin translocation to their favor. The absence of intertissular interactions in dicots probably reflects a better balance between the processes of differentiation at somatic and generative tissues into microsporangium compared to monocots.
Subject(s)
Allium/metabolism , Gametogenesis, Plant/genetics , Lilium/metabolism , Nicotiana/metabolism , Allium/cytology , Cell Communication , Cell Nucleus/metabolism , Cell Nucleus/ultrastructure , Chromatin/chemistry , Flowers/cytology , Flowers/metabolism , Lilium/cytology , Meiosis , Pollen/metabolism , Pollen/ultrastructure , Nicotiana/cytologyABSTRACT
The bipolar spindle is a major cytoskeletal structure, which ensures an equal chromosome distribution between the daughter nuclei. The spindle formation in animal cells depends on centrosomes activity. In flowering plant cells the centrosomes have not been identified as definite structures. The absence of these structures suggests that plants assemble their spindle via novel mechanisms. Nonetheless, the cellular and molecular mechanisms controlling the cytoskeleton remodeling during the spindle development in plants are still insufficiently clear. This article describes the results of a comparative analysis of the microtubular cytoskeleton dynamics during assembly of the second division spindle in tobacco microsporocytes with the normal and deformed nuclei. According to our observations, the bipolar spindle fibres are formed from short arrays of the disintegrated perinuclear cytoskeleton system, the perinuclear microtubular band. The microsporocytes of polyploid tobacco plants with deformed nuclei entirely lack this cytoskeleton structure. In such type of cells the overall prometaphase events are blocked, and the assembly of second division spindles is completely arrested.