ABSTRACT
Two new biflavonoids, pyranoamentoflavone 7-methyl ether (1) and pyranoamentoflavone 4'-methyl ether (2), have been isolated from the leaves of Calophyllum venulosum. The structures of these two new compounds were elucidated by spectroscopic data.
Subject(s)
Calophyllum/chemistry , Flavonoids/isolation & purification , Plants, Medicinal/chemistry , Chemical Phenomena , Chemistry, Physical , Chromatography, High Pressure Liquid , Flavonoids/chemistry , Malaysia , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Plant Leaves/chemistry , Spectrophotometry, Infrared , Spectrophotometry, Ultraviolet , Spectroscopy, Fourier Transform InfraredABSTRACT
[reaction: see text] The structures of novel gaudichaudiic acids F-I (1-4), isolated from the bark of Indonesian Garcinia gaudichaudii, have been elucidated by detailed spectral analysis. Gaudichaudiic acid I (4) is probably derived from 1 as a result of allylic oxidation at C-24 and C-21, followed by aromatization.
Subject(s)
Antineoplastic Agents, Phytogenic/chemistry , Bridged-Ring Compounds/chemistry , Plants, Medicinal/chemistry , Xanthenes/chemical synthesis , Antineoplastic Agents, Phytogenic/isolation & purification , Bridged-Ring Compounds/isolation & purification , Indonesia , Magnetic Resonance Spectroscopy , Mass Spectrometry , Plant Epidermis/chemistry , Spectrometry, Mass, Fast Atom Bombardment , Xanthenes/isolation & purificationABSTRACT
The isolation and identification of a new phloroglucinol derivative (2), a diterpenol (4), together with the known compounds flavesone (1) and sarothralen B (3), from the aerial parts of Hypericum japonicum are reported. Their structures were established by extensive spectral analysis and the structure of (3) has also been confirmed by a single crystal X-ray determination.
Subject(s)
Hypericum/chemistry , Phloroglucinol/analogs & derivatives , Phloroglucinol/chemistry , Plants, Medicinal , Crystallography, X-Ray , Drugs, Chinese Herbal/chemistry , Magnetic Resonance Spectroscopy , Phloroglucinol/isolation & purificationABSTRACT
Three new pyranoxanthones, dulxanthones F-H (1-3), have been isolated from the leaves of Garcinia dulcis. Their structures have been determined on the basis of extensive NMR studies.
Subject(s)
Plants, Medicinal/chemistry , Xanthines/isolation & purification , Molecular Structure , Spectrum Analysis , Xanthines/chemistryABSTRACT
[formula: see text] The structures of sampsoniones I and J, isolated from the aerial parts of the Chinese medicinal plant Hypericum sampsonii, have been elucidated by detailed spectral analysis. They are complex adamantyl derivatives, and sampsonione I is the first polyprenylated benzoylphloroglucinol derivative with the unique caged tetracyclo[7.3.1.1.0]tetradecane-2,12,14-trione skeleton. Cytotoxic sampsonione I has also been obtained by the biomimetic transformation of sampsonione J.
Subject(s)
Adamantane/analogs & derivatives , Hypericum/chemistry , Plants, Medicinal , Adamantane/chemistry , Magnetic Resonance Spectroscopy , Mass Spectrometry , Molecular Mimicry , Molecular StructureABSTRACT
This is the first inn vitro study on the activity of 20 kinds of crude extracts of traditional Chinese medicine (TCM) on the intestinal parasite, Blastocystis hominis using the criteria of living cell count (LCC) and living cell rate (LCR). LCC and LCR were applied as observation indicators, the former as a fixed-quantity and the latter as a fixed-quality method. LCR calculated percentage rate of living cells using eosin-brilliant cresyl blue staining which could differentiate between living cells and dying or dead cells. There were five extracts with no inhibitory activity, thirteen with moderate inhibition and two with high inhibition. The crude extracts of Coptis chinensis (CC) and Brucea javanica (BJ) were found to be most active against B. hominis. The active concentration of CC was 100 micrograms/ml. The active concentration of BJ was 500 micrograms/ml. The active concentration of metronidazole (MD) was 10 micrograms/ml and this was taken as an active standard drug for B. hominis.
Subject(s)
Blastocystis hominis/drug effects , Medicine, Chinese Traditional , AnimalsABSTRACT
The methanol extract from the whole plant of Geum japonicum was found to inhibit the human immunodeficiency virus (HIV-1) protease. Through bioassay-directed fractionation of the extract, a new triterpene acid along with five known triterpene acids, ursolic acid, epipomolic acid, maslinic acid, euscaphic acid, and tormentic acid, were isolated. The structure of the new compound was determined by spectral means including 1H-1H COSY, HMQC, HMBC, and NOE experiments to be 2 alpha, 19 alpha-dihydroxy-3-oxo-12-ursen-28-oic acid (1). Of these compounds, 1, ursolic acid, and maslinic acid showed potent inhibitory activity against HIV-1 protease.
Subject(s)
Antiviral Agents/pharmacology , HIV Protease Inhibitors/pharmacology , HIV-1/drug effects , HIV-1/enzymology , Plants, Medicinal/chemistry , Triterpenes/pharmacology , Amino Acid Sequence , Antiviral Agents/chemistry , HIV Protease Inhibitors/chemistry , Humans , Magnetic Resonance Spectroscopy , Molecular Sequence Data , Triterpenes/chemistryABSTRACT
A novel derivative of sucrose, beta-(3,6-di-O-feruloyl)-fructofuranosyl-alpha-(2,3,4,6-tetra-O-ac etyl)- glucopyranoside, was isolated from the wood of Bhesa paniculata. Its structure was determined by a combination of 2D 1H-1H and 1H-13C correlation NMR spectroscopy. The known compounds, glycerol 1-9',12'-octadecadienoate, beta-sitosterol, (+/-)-pinoresinol, methyl 3,4-dihydroxybenzoate, 4-hydroxy-3-methoxybenzoic acid, anofinic acid and 2-(1'-methylethenyl)-benzofuran-5-carboxylic acid were also isolated.
Subject(s)
Coumaric Acids/chemistry , Plants, Medicinal/chemistry , Sucrose/analogs & derivatives , Trees/chemistry , Carbohydrate Conformation , Carbohydrate Sequence , Chromatography, High Pressure Liquid/methods , Coumaric Acids/analysis , Coumaric Acids/isolation & purification , Indonesia , Magnetic Resonance Spectroscopy , Malaysia , Models, Structural , Molecular Sequence Data , Sucrose/chemistry , Sucrose/isolation & purificationABSTRACT
A substance with antiproliferative bioactivity in an aqueous extract of Cordyline terminalis was purified and identified by mass spectrometry to be the natural nucleoside, thymidine. 10(-5) M Thymidine inhibited EL4 cell replication and decreased cell viability after 12-24 h. The effect was highly specific for this nucleoside. Treated cell cultures showed a significant increase in S phase cells and a corresponding decrease in G1 phase cells. Nitrobenzylthioinosine (which prevented facilitated entry of thymidine) protected cells from the antiproliferative action of thymidine. A human breast cancer cell line (MCF7) was also growth-inhibited by 10(-5) M thymidine but a murine lymphoma cell line (K36) was not. Thus, submillimolar thymidine has effects on cell proliferation which are selective both with respect to specificity for the compound and for different tumour cell lines.