Depolymerization of holocellulose from Chinese herb residues by the mixture of lignin-derived deep eutectic solvent with water.

The depolymerization of biomass carbohydrate polymers usually happened in homogeneous medium, just a few in heterogeneous solution. Herein, holocellulose from two Chinese herb residues Cortex albiziae (HRCA) and Heteropogon contortus (HRHC) was prepared and characterized. Deep eutectic solvent (DES) of choline chloride/p-coumaric acid collaborating with water was employed for the dissociation of those two holocelluloses and selectively dissolved hemicellulose into xylose with maximal yields of 81.50 % and 72.47 %, respectively. Most cellulose remained as the polymer state with a maximum solubility of 9.38 %. The synergistic action of DES and water was investigated to unveil the depolymerization mechanism and the roles of each component in depolymerizing procedure. It was water that released hemicellulose polymers and dissolved polymers to soluble short oligosaccharides, and DES rapidly cleaved the latter to xylose. Contrarily, DES broke cellulose to only soluble cello-oligosaccharides, but water exhibited the ability to hydrolyze cellulose to glucose.

[Soil fertility characteristics and their influencing factors in tea plantations of Jiangxi Pro-vince, China]. / æ±Ÿè¥¿çœèŒ¶å›­åœŸå£¤è‚¥åŠ›ç‰¹å¾åŠå ¶å½±å“å› å­.

To investigate soil fertility status and characteristics of typical tea plantations, we selec-ted 372 typical tea plantations of 21 areas across Jiangxi Province and analyzed the soil nutrient, spatial data, and their correlations with topography, soil type, elevation and plantation age. The results showed that soil pH, organic matter, alkaline nitrogen, available phosphorus, available potassium, total nitrogen, total phosphorus and total potassium of tea plantation in Jiangxi reached 53.9%, 60.1%, 56.1%, 22.9%, 38.5%, 43.7%, 11.1% and 95.5% of indices of high fertility, high efficiency and high yield tea plantation, respectively, with the available phosphorus showing a strong variation. Soil available copper, zinc, iron, manganese and boron reached 76.3%, 74.2%, 96.8%, 73.1% and 0.0% of the first-class standards for soil trace elements, respectively. Tea plantations with highest soil fertility located in central Jiangxi, followed by northeastern and northwestern Jiangxi, and lowest in southern Jiangxi. Soil pH was significantly positively correlated with organic matter, alkaline nitrogen, available phosphorus, available potassium, total nitrogen and total phosphorus but not for total potassium. For different topography, soil fertility was highest in the flat land, followed by the high mountains, and lowest in the mountains and hills. Across different soil types, soil fertility was higher in paddy soil, sandy soil and mountain yellow brown soil, followed by yellow soil, red-yellow soil and purple soil, and lowest in red soil. Soil pH, organic matter and total potassium increased while available phosphorus decreased with altitude. The organic matter, alkaline nitrogen, available phosphorus, total nitrogen and total phosphorus increased, but soil pH decreased with time. In summary, soil fertility of tea plantations in Jiangxi Province was generally good, with high organic matter, total potassium, available copper, zinc, iron and manganese. However, soil was acidic, available phosphorus and total phosphorus content was low, available boron was seriously limited. We suggest increase soil pH and potassium supply in central Jiangxi, increase potassium and nitrogen fertilizer supply in northeastern Jiangxi, increase organic matter and phosphorus fertilizer supply in northwestern Jiangxi, and increase nitrogen, phosphorus and potassium supply combined with organic fertilizers in southern Jiangxi. High mountain tea plantations should enhance available phosphorus and potassium supply. Mountain tea plantations should enhance nitrogen and phosphate supply. Tea plantations with red and yellow soil should increase pH and total potassium supply. Tea plantations with red soil should apply nitrogen, phosphorus and potassium fertilizers combined with organic fertilizers. Tea plantations with yellow soil and mountain yellow brown soil required additional phosphorus supply, and tea plantations with purple soil should increase soil organic matter supply. Tea plantations need to increase dolomite powder, physiological alkaline fertilizers and organic fertilizers to prevent soil acidification.

Combination Therapy With Hyperbaric Oxygen and Erythropoietin Inhibits Neuronal Apoptosis and Improves Recovery in Rats With Spinal Cord Injury.

BACKGROUND: Apoptosis plays an important role in various diseases, including spinal cord injury (SCI). Hyperbaric oxygen (HBO) and erythropoietin (EPO) promote the recovery from SCI, but the relationship between apoptosis and the combination therapeutic effect is not completely clear. OBJECTIVE: The purpose of this study was to investigate the effects of HBO and EPO on SCI and the mechanisms that underlie their therapeutic benefits. DESIGN: The study was designed to explore the effects of HBO and EPO on SCI through a randomized controlled trial. METHODS: Sixty young developing female Sprague-Dawley rats were randomly divided into groups of 12 rats receiving sham, SCI, HBO, EPO, or HBO plus EPO. The SCI model was modified with the Allen method to better control consistency. HBO was performed for 1 hour per day for a total of 21 days, and EPO was given once per week for a total of 3 weeks. Both methods were performed 2 hours after SCI. Locomotor function was evaluated with the 21-point Basso-Beattie-Bresnahan Locomotor Rating Scale, an inclined-plane test, and a footprint analysis. All genes were detected by Western blotting and immunohistochemistry. The level of cell apoptosis was determined by Hoechst staining. RESULTS: The results showed that HBO and EPO promoted the recovery of locomotor function in the hind limbs of rats by inhibiting the apoptosis of neurons. During this period, the expression of B-cell lymphoma/leukemia 2 protein (Bcl-2) increased significantly, whereas the expression of Bcl-2-associated X protein (Bax) and cleaved caspase 3 decreased significantly, indicating the inhibition of apoptosis. Meanwhile, the expression of G protein-coupled receptor 17 decreased, and that of myelin basic protein increased, suggesting that there may be a potential connection between demyelination and neuronal apoptosis. LIMITATIONS: The limitations of the study include deviations in the preparation of SCI models; lack of reverse validation of molecular mechanisms; absence of in vitro cell experiments; and only one time point after SCI was studied. CONCLUSIONS: HBO and EPO treatments are beneficial for SCI, especially when the 2 therapies are combined.

Hyperbaric Oxygen Improves Functional Recovery of the Injured Spinal Cord by Inhibiting Inflammation and Glial Scar Formation.

BACKGROUND: Inflammation and glial scar formation determine the recovery process after spinal cord injury. Hyperbaric oxygen is used as a rehabilitation therapy for various clinical diseases, including spinal cord injury. However, the relationship between hyperbaric oxygen therapy and inflammation or glial scar is not fully understood. OBJECTIVE: The aim of this study was to investigate the therapeutic effect and molecular mechanism of hyperbaric oxygen on spinal cord injury. METHODS: A total of 54 developing female Sprague-Dawley rats were randomly divided into sham group, spinal cord injury group, and hyperbaric oxygen group, with 18 rats in each group. The model of spinal cord injury was established using Allen's method. Hyperbaric oxygen therapy was administered once a day until the rats were killed. RESULTS: The results demonstrated inflammation and glial scar formation are involved in secondary spinal cord injury. After hyperbaric oxygen treatment, there was a notable improvement of the locomotor function in rats. Hyperbaric oxygen reduced the inflammatory reaction and glial scar formation by inhibiting inflammation-related factors iNOS and COX-2 and glial scar-related components GFAP and NG2. This process may be achieved by inhibiting AKT and NF-kB pathways. CONCLUSIONS: Hyperbaric oxygen effectively promotes the recovery of spinal cord injury by inhibiting inflammation and glial scar formation.

Hyperbaric oxygen treatment of spinal cord injury in rat model.

BACKGROUND: The purpose of this study was to investigate the therapeutic effects and mechanisms of hyperbaric oxygen (HBO) treatment on rats following spinal cord injury (SCI). METHODS: A total of 45 Sprague-Dawley (SD) rats were randomly divided into three groups. Sham-SCI group was surgically exposed but not subjected to the SCI procedure. SCI-control group was administered SCI and treated with regular air. SCI-HBO group was administered SCI and HBO treatment. Neuromotor functions were examined using the Basso, Beattie, and Bresnahan (BBB) locomotor rating scale and the inclined plane assessment at before SCI (baseline) and after SCI. Superoxide dismutase (SOD) activities and malondialdehyde (MDA) levels were measured. RESULTS: Starting from Day 1 after SCI but except Day 2, the SCI-HBO group has significantly higher BBB scores than the SCI-control group. After SCI, the maximum inclination angles at which rats could maintain were significantly lower in both SCI groups. But the maximum angles were significantly bigger for the rats in the SCI-HBO group than those on the SCI-control group at 5, 10 and 20 days after SCI. SOD activities in SCI-HBO rats were significantly higher and MDA levels were significantly lower than in SCI-control rats, at two and five days after SCI. There was also less cystic degeneration of spinal cord in SCI-HBO rats, compared to SCI-control rats. CONCLUSIONS: These results suggest that HBO treatment has a therapeutic value in treating SCI. Increased oxygen free radical scavenging and reduced lipid oxidation may be one of the mechanisms.

Changes in autophagy in rats after spinal cord injury and the effect of hyperbaric oxygen on autophagy.

The purpose of this study was to explore the effects of Hyperbaric oxygen (HBO) on the autophagic changes after induction of spinal cord injury (SCI) in rats. A total of 75 rats were randomly divided into the sham-operated group, the spinal cord injury group, and the SCI+HBO group. We found that at 7 d and 14 d after surgery, the BBB scores were higher in the SCI+HBO group in comparison to the SCI group. The expression of Beclin-1 and LC3II was upregulated in the SCI and SCI+HBO groups after SCI. Fluorescently stained Beclin-1 and LC3II proteins were barely detectable in the sham group. In contrast, Beclin-l and LC3II expression was observed in neurons and glial cells from the SCI and SCI+HBO groups. Beclin-1 and LC3II expression appeared at 6h after SCI. At each time point, Beclin-1 and LC3II expression was significantly higher in the SCI+HBO group compared to the SCI group. These results suggest that autophagy is activated in rats after SCI and sustained over a period of time. HBO treatment enhances autophagy expression in rats after SCI and accelerates cell repair rate, which may represent one of the mechanisms of action of HBO in the treatment of SCI.

Microalgae consortia cultivation in dairy wastewater to improve the potential of nutrient removal and biodiesel feedstock production.

The potential of microalgae consortia used in dairy wastewater treatment combined with microalgae biodiesel feedstock production was evaluated by comparing the nutrient removal of dairy wastewater, the growth of cells, and the lipid content and composition of biomass between monoalgae and microalgae consortia cultivation system. Our results showed that higher chemical oxygen demand (COD) removal (maximum, 57.01-62.86 %) and total phosphorus (TP) removal (maximum, 91.16-95.96 %) were achieved in almost microalgae consortia cultivation system than those in Chlorella sp. monoalgae cultivation system (maximum, 44.76 and 86.74 %, respectively). In addition, microalgae consortia cultivation except the mixture of Chlorella sp. and Scenedesmus spp. reached higher biomass concentration (5.11-5.41 g L(-1)), biomass productivity (730.4-773.2 mg L(-1) day(-1)), and lipid productivity (143.7-150.6 mg L(-1) day(-1)) than those of monoalgae cultivation (4.72 g L(-1), 674.3, and 142.2 mg L(-1) day(-1), respectively) on the seventh day. Furthermore, the fatty acid methyl ester (FAME) profiles indicated the lipids produced from microalgae consortia cultivation system were more suitable for biodiesel production. The microalgae consortia display superiority in dairy wastewater treatment and the getting feedstock for biodiesel production.

Addition of optically pure H-phosphinate to ketones: selectivity, stereochemistry and mechanism.

Aromatic methyl ketones and cyclic asymmetric ketones underwent hydrophosphorylation with P-stereogenic H-P species in the presence of potassium carbonate to produce P,C-stereogenic tertiary α-hydroxyl phosphinates in excellent yields with up to 99 : 1 dr. The diastereoselectivity was induced by a reversible conversion of less stable stereomer of product to that of a more stable one via an equilibrium, which was confirmed by aldehyde/ketone exchanging reaction. Toward the exchange, aliphatic or aldehyde carbonyl were more active than aromatic or ketone carbonyls, respectively. The stability difference between the two diastereomers was controlled by the sizes of substituents linking to phosphorus or α-carbon.

Analysis of gene expression changes in relation to toxicity and tumorigenesis in the livers of Big Blue transgenic rats fed comfrey (Symphytum officinale).

BACKGROUND: Comfrey is consumed by humans as a vegetable and a tea, and has been used as an herbal medicine for more than 2000 years. Comfrey, however, is hepatotoxic in livestock and humans and carcinogenic in experimental animals. Our previous study suggested that comfrey induces liver tumors by a genotoxic mechanism and that the pyrrolizidine alkaloids in the plant are responsible for mutation induction and tumor initiation in rat liver. RESULTS: In this study, we identified comfrey-induced gene expression profile in the livers of rats. Groups of 6 male transgenic Big Blue rats were fed a basal diet and a diet containing 8% comfrey roots, a dose that resulted in liver tumors in a previous carcinogenicity bioassay. The animals were treated for 12 weeks and sacrificed one day after the final treatment. We used a rat microarray containing 26,857 genes to perform genome-wide gene expression studies. Dietary comfrey resulted in marked changes in liver gene expression, as well as in significant decreases in the body weight and increases in liver mutant frequency. When a two-fold cutoff value and a P-value less than 0.01 were selected, 2,726 genes were identified as differentially expressed in comfrey-fed rats compared to control animals. Among these genes, there were 1,617 genes associated by Ingenuity Pathway Analysis with particular functions, and the differentially expressed genes in comfrey-fed rat livers were involved in metabolism, injury of endothelial cells, and liver injury and abnormalities, including liver fibrosis and cancer development. CONCLUSION: The gene expression profile provides us a better understanding of underlying mechanisms for comfrey-induced hepatic toxicity. Integration of gene expression changes with known pathological changes can be used to formulate a mechanistic scheme for comfrey-induced liver toxicity and tumorigenesis.

Gene expression profiles distinguish the carcinogenic effects of aristolochic acid in target (kidney) and non-target (liver) tissues in rats.

BACKGROUND: Aristolochic acid (AA) is the active component of herbal drugs derived from Aristolochia species that have been used for medicinal purposes since antiquity. AA, however, induced nephropathy and urothelial cancer in people and malignant tumors in the kidney and urinary tract of rodents. Although AA is bioactivated in both kidney and liver, it only induces tumors in kidney. To evaluate whether microarray analysis can be used for distinguishing the tissue-specific carcinogenicity of AA, we examined gene expression profiles in kidney and liver of rats treated with carcinogenic doses of AA. RESULTS: Microarray analysis was performed using the Rat Genome Survey Microarray and data analysis was carried out within ArrayTrack software. Principal components analysis and hierarchical cluster analysis of the expression profiles showed that samples were grouped together according to the tissues and treatments. The gene expression profiles were significantly altered by AA treatment in both kidney and liver (p < 0.01; fold change > 1.5). Functional analysis with Ingenuity Pathways Analysis showed that there were many more significantly altered genes involved in cancer-related pathways in kidney than in liver. Also, analysis with Gene Ontology for Functional Analysis (GOFFA) software indicated that the biological processes related to defense response apoptosis and immune response were significantly altered by AA exposure in kidney, but not in liver. CONCLUSION: Our results suggest that microarray analysis is a useful tool for detecting AA exposure; that analysis of the gene expression profiles can define the differential responses to toxicity and carcinogenicity of AA from kidney and liver; and that significant alteration of genes associated with defense response, apoptosis and immune response in kidney, but not in liver, may be responsible for the tissue-specific toxicity and carcinogenicity of AA.