1.
Methods Mol Biol
; 2791: 89-96, 2024.
Article
in English
| MEDLINE
| ID: mdl-38532095
ABSTRACT
Culture of immature buckwheat embryos is an excellent explant for tissue culture and regeneration in vitro, as well as a method of embryo rescue in case of fruit abortion especially in interspecific crosses. Here, we describe the method of immature embryo rescue using a differentiated approach to different age groups. The method involves the development of embryos along the path of direct embryogenesis bypassing the callus stage.