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Therapeutic Methods and Therapies TCIM
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Acta Neurobiol Exp (Wars) ; 77(1): 45-56, 2017.
Article in English | MEDLINE | ID: mdl-28379215

ABSTRACT

Traditionally, Morus rubra L. (Moraceae) (red mulberry) and Cornus mas L. (Cornacea) (cornelian cherry) fruits are eaten fresh and are also used in marmalades, juices, jam, natural dyes in Turkey and are believed to have beneficial effects in case of multiple health issues such as antipyretic, diarrhea and intestinal parasites. However, the effects of M. rubra and C. mas on epilepsy has not been known. This study evaluates the effects of M. rubra and C. mas extracts on penicillin-induced epileptiform activity. Sixty Wistar rats randomly divided into ten groups (n=6): control, sham, penicillin, penicillin+M. rubra extract (2.5, 5, 10, 20 mg/kg) and penicillin+C. mas extract (2.5, 5, 10 mg/kg). Epileptiform activity was induced by using penicillin (500 IU, i.c.) and electrocorticogram records (150 min) were obtained. Also, biochemical analysis in blood samples were evaluated. According to the electrocorticogram analysis, the effective dose was detected as 10 mg/kg for both C. mas and M. rubra. This dose decreased the spike frequencies of convulsions while amplitude wasn't changed by both substances. In erythrocyte studies, there were significant differences regarding nitric oxide in the control, sham and penicillin groups. There were significant differences regarding malondialdehyde in all groups. In the plasma, there were significant differences among groups regarding xanthine oxidase in the penicillin­C. mas and penicillin­M. rubra groups. There were differences regarding malondialdehyde in the penicillin-C. mas and M. rubra-C. mas groups. Both extracts reduced the frequency of epileptiform activity. After administration of the extracts malondialdehyde levels decreased also in both erythrocytes and plasma.


Subject(s)
Epilepsy/drug therapy , Erythrocytes/metabolism , Glucosides/chemistry , Morus/chemistry , Plant Extracts/therapeutic use , Pyrans/chemistry , Action Potentials/drug effects , Animals , Anti-Bacterial Agents/toxicity , Anticonvulsants/therapeutic use , Brain Waves/physiology , Cerebral Cortex/pathology , Disease Models, Animal , Epilepsy/blood , Epilepsy/chemically induced , Erythrocytes/drug effects , Male , Neurons/drug effects , Nitric Oxide/blood , Penicillins/toxicity , Rats , Rats, Wistar , Superoxide Dismutase/blood , Thiazolidinediones/blood , Time Factors
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