Acupunto Renzhong en el caballo. Estudio anatómico e histológico / Acupoint renzhong (jenchung GV-26) in the horse. Anatomical and histological study

El propósito de este estudio es documentar las características histológicas y anatómicas del acupunto Renzhong GV-26 en diez caballos de raza española. Primero, se midió la resistencia eléctrica para la localización del acupunto, y posteriormente se insertó una aguja de acupuntura a una profundidad de 1 cm dentro del acupunto. Luego se obtuvieron muestras del acupunto y fueron procesadas por técnicas histológicas de rutina. Los resultados muestran que el acupunto Renzhong GV-26 está situado en el estrato intermedio del labio superior, representado por diferentes estructuras, como tejido conectivo denso, músculo estriado esquelético, fibras nerviosas y estructuras vasculares. Es decir, numerosas estructuras conforman el acupunto Renzhong GV-26 en el caballo (AU)

The purpose of this study was to document the anatomic and histologic characteristics of acupoint Renzhong GV-26 in ten Spanish horses. First, the electrical skin resistance was measured to locate the acupoint, and the needles were inserted to a depth of 1cm into the acupoint. Then, the tissue surrounding the acupoint was removed, sectioned serially, and processed by routine histologic techniques. The results show that the acupoint Renzhong GV-26 is situated in the intermediate area of the upper lip, represented by several structures of dense connective tissue, skeletal muscle tissue, nerve fibers and vascular structures. Therefore, numerous structures conform part of the acupoint Renzhong GV-26 in the horse (AU)

Inhibition of voltage-gated cationic channels in rat embryonic hypothalamic neurones and C1300 neuroblastoma cells by triphenylethylene antioestrogens.

The effect of the non-steroidal antioestrogens tamoxifen and toremifene on voltage-gated cationic currents was examined in primary cultures of rat hypothalamic neurones and the C1300 mouse neuroblastoma cell line using the whole-cell patch clamp technique. When applied to the external bathing solution both tamoxifen and toremifene were able to inhibit TTX-sensitive sodium currents with IC50 values of 1-2 microM and delayed rectifier type potassium currents (IC50, 2-3 microM). However, only toremifene showed a significant inhibition of the I(A) current (IC50 3 microM). Inhibition of voltage-gated cationic currents was significantly impaired when tamoxifen was applied in a serum-containing solution. The steroidal antioestrogen ICI 182,780 did not inhibit any of the currents at 10 microM.