Anti-fungal efficacy of Miswak Extract (Salvadora Persica) and commercial cleaner against Candida albicans on heat cured polymethylmethacrylate denture base.

AIM: The aim of the study was to compare the antifungal efficacy between commercial cleaner (Corega) and Miswak extract (Salvadora persica) against Candida albicans on heat cured Polymethyl methacrylate (PMMA) acrylic denture base resin. MATERIALS AND METHODS: Forty-eight samples of heat cured PMMA acrylic denture base resin were fabircated in the study. The sterile acrylic resin specimens were immersed in standardized cell suspension of Candida albicans and incubated for 60 min at 370°C for cell adhesion and 2 h at 370°C for biofilm formation. After 24 h biofilm was evaluated by cell viability (CFUs) on SDA and cell counting of Candida albicans under light microscope at 400× magnification. The fungicidal effect of commercial cleaner and Miswak extract on Candida albicans biofilm was then evaluated by colony-forming units on SDA and cell counting under light microscope at 400× magnification. RESULTS: Screening test agar disk-diffusion assay showed mean inhibitory zone of 3 mm for commercial cleaner as compared to Miswak extract, which showed mean inhibitory zone of 2 and 1 mm for different concentrations. Broth microdilution method showed 31 mg/ml MIC and 62.5 mg/ml Minimal Fungicidal Concentration (MFC) values for commercial cleaner as compared to Miswak extract that showed 125 mg/ml MIC and 250 mg/ml MFC values against Candida albicans. A significant difference (p < 0.05) was observed between pre and post treatment of both commercial cleaner and Miswak extract, for CFUs and cell count for Candida albicans. CONCLUSION: Commercial denture cleaner (Corega) showed better antifungal (C albicans) activity than Miswak extract (Salvadora persica) on heat cured PMMA acrylic denture base resin.

Decontamination of lithium disilicate ceramics using various photosensitizers, herbal and chemical disinfectants, and the effect of surface conditioners on bond strength values.

AIM: To assess and compare the antimicrobial efficacy of disinfectants on lithium disilicate ceramic (LDC) used in dental applications and shear bond strength (SBS) of LDC after using different conditioners hydrofluoric acid (HF), self-etching ceramic primers (SECP) and Neodymium-doped yttrium orthovanadate (Nd: YVO4). MATERIALS AND METHODS: One hundred and twenty LDC discs were fabricated by auto-polymerizing acrylic resin using the lost wax technique. S. aureus, S. mutans, and C. albican were inoculated on thirty discs (n = 30 each). Each group was further divided into three subgroups based on different disinfecting agents used (n = 30) Group 1: Garlic extract, Group 2: Rose Bengal (RB) activated by PDT, and Group 3: Sodium hypochlorite (NaOCl). An assessment of the survival rate of microorganisms was performed. The remaining thirty samples were surface treated using three different LDC surface conditioners (n = 10) Group 1: HF + Silane (S), group 2: SECP, and Group 3: Nd: YVO4 laser+S. SBS and failure mode analysis were performed using a universal testing machine and stereomicroscope at 40x magnification, The statistical analysis was conducted using one-way ANOVA and Post Hoc Tukey test. RESULTS: Garlic extract, RB, and 2% NaOCl sample displayed comparable outcomes of antimicrobial potency against C. albicans, S aureus, and S. mutans (p > 0.05). Furthermore, SBS analysis showed HF+S, SECP, and Nd: YVO4+S exhibited comparable outcomes of bond strength (p > 0.05). CONCLUSION: Garlic extract and Rose bengal activated by PDT can be contemplated as alternatives to the chemical agent NaOCl used for LDC disinfection. Similarly, SECP and Nd: YVO4 possess the potential to be used for the surface conditioning of LDC to improve the bond integrity with resin cement.

Comparison of the postoperative anti-inflammatory efficacy of chlorhexidine, saline rinses and herbal mouthwashes after mechanical debridement in patients with peri-implant mucositis: A randomized controlled trial.

AIM: The null hypothesis is that there is no difference in the post-operative anti-inflammatory efficacy of chlorhexidine (CHX), 2% saline rinses (SR) and a herbal mouthwash (MW) after non-surgical mechanical debridement (MD) for treatment of peri-implant mucositis (PiM). The aim was to compare the post-operative anti-inflammatory efficacy of CHX, 2% SR and a herbal oral rinse after non-surgical MD of PiM. MATERIALS AND METHODS: The present randomized controlled trial had a single-blinded parallel arm design. Patients diagnosed with PiM were enrolled. Demographic information was recorded. All patients underwent MD and were randomly divided into 4 groups: CHX-group: 0.12% non-alcoholic CHX; Sodium chloride (NaCl) group: 2% NaCl rinses; Herbal MW group: Herbal-based MW and H2 O group: distilled water with peppermint flavour. After MD, all the participants were advised to rinse twice daily (every 12 hrs) for 2 weeks with their respective MWs. In all groups, peri-implant modified plaque index (mPI), modified gingival index (mGI) and probing depth (PD) were measured at baseline and at 12 weeks of follow-up. Sample size was estimated using data from a pilot investigation; and group-comparisons were performed. Statistical significance was confirmed when P-values were below 0.01. RESULTS: Sixty individuals (15 patients/group) were included. At baseline, mPI, mGI and PD were comparable in all groups. At baseline, there was no significant difference in peri-implant mPI, mGI and PD in all groups. At 12-weeks' follow-up, there was a statistically significant reduction in peri-implant mPI (p < 0.01), mGI (p < 0.01) and PD (p < 0.01) in CHX, NaCl and herbal MW groups compared with H2 O group. There was no significant relation between implant location, duration for which, implants were functional, gender and peri-implant clinical parameters in all groups. CONCLUSION: After non-surgical MD, post-operative use of CHX and herbal and NaCl MWs is useful for the management of PiM in the short-term.

Efficacy of chlorhexidine, photosensitizers, green tea extract, and propolis on bond integrity and microleakage of caries-affected dentin: An in-vitro study.

AIM: The current study aimed to evaluate the efficacy of chlorhexidine (CHX), phycocyanin photosensitizer (PC), green tea extract (GTE), and propolis (PP) on the adhesive bond integrity and microleakage of caries-affected dentin (CAD) using etch and rinse adhesive system. MATERIAL AND METHODS: A sum of 80 extracted human mandibular molars was collected and assessed using ICDAS criteria. Randomly CAD samples were treated with different disinfectants (n = 20) each CHX, PC, GTE, and PP. After ensuring disinfection, specimens were rehabilitated with bulk-fill composite resin employing etch and rinse adhesive system. SBS testing was performed by engaging (n=10) specimens in a universal testing machine. Microleakage analysis was performed by using the dye penetration technique.A stereomicroscope under 40× magnification was utilized for analyzing failure modes. Statistical analysis was accomplished by using the ANOVA and Tukey's post hoc tests (p<0.05). RESULTS: Highest bond strength was displayed by CAD disinfection with CHX and bonded to resin cement (15.33 ± 0.14 MPa). Whereas, the lowest SBS bond value was presented by Group 2 dentin surface disinfection with PC. CAD disinfected with GTE, PP and CHX demonstrated comparable SBS(p>0.05). The maximum microleakage score was exhibited when CAD bonded to resin cement disinfected with PP. While the minimum microleakage score was unveiled CAD bonded to resin cement sanitized with CHX CONCLUSION: Caries-affected dentin bonded to resin cement, chlorhexidine exhibited the highest adhesive shear bond strength with a minimal microleakage score using etch and rinse adhesive system.

A Comparative Study on Alvogyl and a Mixture of Black Seed Oil and Powder for Alveolar Osteitis: A Randomized Double-Blind Controlled Clinical Trial.

Introduction: Alveolar osteitis (AO) is the most common complication faced by exodontia patients and is usually seen 24-74 hours after tooth extraction, heralded by severe throbbing pain. Nigella sativa is commonly known as black seed known to have anti-inflammatory and antibacterial properties along with other reparative properties that enhance bone formation. This study aimed to evaluate and compare the effects of Alvogyl and a mixture of Nigella sativa powder and oil in the treatment of dry sockets. Materials and Methods: Sixty patients above the age of 18 and below 70 years, from both genders, who underwent extraction of teeth and were clinically diagnosed with a dry socket at the clinic of the College of Dentistry, Jouf University, Saudi Arabia, were included in this study. Pain scores were assessed after placement of the dressing at the following intervals: 5 minutes, 30 minutes, 60 minutes, 2nd day, 4th day, and 7th day. Patients were randomly allocated to three groups, namely, Group 1 (Alvogyl), Group 2 (mixture of Nigella sativa's powder and oil), and Group 3 (control). Pain relief and healing of the socket were compared between the three groups. The collected data were subjected to statistical analysis through Spearman's correlation test, independent t-test, ANOVA, and post hoc test. Results: A mixture of Nigella sativa powder and oil showed a statistically significant difference in relieving pain compared to the Alvogyl group. A mixture of Nigella sativa's powder and oil required fewer dressings when compared to the Alvogyl group. Conclusion: A mixture of Nigella sativa powder and oil is the more efficacious dressing material for the management of dry sockets compared to Alvogyl. It provides immediate and complete pain relief and fewer numbers of repeated visits.

Analgesic Efficacy of Curcuma longa (Curcumin) after Surgical Periodontal Therapy.

PURPOSE: To compare the analgesic efficacy of orally administered Curcuma longa (curcumin) and mefenamic acid (MA) after surgical periodontal therapy (SPT). MATERIALS AND METHODS: Seventy-six periodontitis patients were randomly divided into two groups. In the test group, patients received curcumin capsules (200 mg), and in the control group, patients received MA (500 mg). All patients underwent post-operative antibiotic therapy using 500 mg amoxicillin and 400 mg metronidazole for 7 days. Post-operative pain and discomfort were evaluated using the numerical rating scale (NRS) and verbal rating scale (VRS), respectively. Evaluation were performed after 24 (T1), 48 (T2), and 72 h (T3). Group comparisons were done using Student's t-test and the Mann-Whitney U-test. The level of statistical significance was established at p < 0.05. RESULTS: All patients had stage 3/grade C periodontitis. The mean age of individuals in the test and control groups were 58.4 ± 7.3 and 57.2 ± 5.2 years, respectively. A family history of periodontal diseases was reported by 37.5% and 47.4% individuals in the test and control groups, respectively. In the test and control groups, the total mean duration of periodontal surgery was 168.2 ± 12.2 and 173.4 ± 10.7 min, respectively. There was no statistically significant difference in the mean NRS and VRS scores among patients in the test and control groups. In both groups, there was no statistically significant difference in the change in NRS scores at any time point. CONCLUSIONS: Compared with MA, curcumin is ineffective for pain and discomfort management after SPT. The possibility of the results being biased due to lack of operator blinding cannot be overlooked.

Disinfection of acrylic denture resin polymer with Rose Bengal, Methylene blue and Porphyrin derivative in photodynamic therapy.

AIM: The study aimed to assess the effect of in-vitro chlorhexidine and antimicrobial photodynamic therapy (aPDT) disinfection protocols against acrylic resin specimens colonized with S. mutans, S. aureus, E. coli, and C. albicans. MATERIAL AND METHODS: Reference strains of S. mutans, S. aureus, E. coli, and C. albicans were tested. Sixteen blocks of acrylic specimens were prepared by heat-cure acrylic resin and contaminated by in-vitro biofilm growth. Specimens in group 1, group 2 and group 3 were treated with Rose Bengal (RB), methylene blue (MB) 500 mg/L and porphyrin derivative (PD) 5 ml respectively, for the sensitization of biofilms. All photosensitizers (PS) were activated by LED at different wavelength. CHX was prepared in sterile distilled water and applied for 60 s. Each contaminated specimen was sprayed on all its surfaces with the aforementioned photosensitizers and control CHX. One-way analysis of variance (ANOVA) model was used to test the effect of the treatments and Tukey multiple comparison tests to compare means OF CFU/mL (log10) for exposed E. coli, C. albicans, S aureus, and S. mutans RESULTS: Specimens treated with 0.12% CHX (control) demonstrated a significant reduction in CFU/mL (log10) for exposed E. coli; 2.04±0.07 CFU/mL, C. albicans; 2.09±0.85 CFU/mL, S aureus; 3.04±0.11 CFU/mL, and S. mutans; 2.54±0.91 CFU/mL. The intragroup comparison revealed E.coli did not exhibit a decrease in reduction CFU/mL (log10) when acrylic resin irradiated with RB 5 µm. Whereas, CFU/mL (log10) values of S.aureus; 3.62±0.68 and S.mutans; 3.41±0.13 plummeted (p<0.05). Intergroup comparison showed E.coli values to display comparable reduction when disinfected with MB 500 mg/L and 0.12% CHX; 3.16±0.34 and 2.04±0.07 CFU/mL (log10) (p<0.05). CONCLUSION: Photosensitizers (RB, MB, PD) are selective in reducing bacterial count on acrylic resin blocks. CHX was found to be effective against all bacteria E.coli, C.albicans, S.aureus, and S.mutans at a concentration of 0.12%.

Influence of adjunctive photodynamic therapy (curcumin) on the extrusion bond strength of radicular dentin to contemporary root canal sealers.

OBJECTIVE: The aim was to compare Photodynamic therapy (PDT) as an adjunct to NaOCl and EDTA on the extrusion bond strength (EBS) of AH Plus (AH), Sealapex (SA) and MTA Fillapex (MF) root canal sealers. MATERIALS AND METHOD: Root canal treatment for human teeth (n = 60) using protaper universal system was performed followed by irrigation with NaOCl/ EDTA. Samples were divided (n = 30) based on disinfection technique, NaOCl/EDTA and NaOCl/EDTA with PDT (Curcumin-PS-CP) as an adjunct. Specimens were divided in three subgroups (n = 10) based on sealers used (Gp 1-Na-E-AH, Gp 2-Na-E-PDT-AH, Gp 3-Na-E-SA, Gp 4-Na-E-PDT-SA, Gp 5-Na-E-MF & Gp 6-Na-E-PDT-MF). Canals were dried and obturated followed by sectioning at coronal and middle third. EBS testing was performed using a universal testing machine (UTM) and modes of failure were assessed. Analysis of variance (ANOVA) and Tukey multiple comparison tests were used to compare the means of EBS. (p < 0.05). RESULTS: Sealers showed a significant influence on EBS, as Gp 1 (Co: 9.21 ± 0.62, Mid: 8.54 ± 0.55) and Gp 2 (Co: 7.11 ± 0.68, Mid: 7.30 ± 0.37) showed higher EBS than Gp 3 (Co: 5.21 ± 0.63, Mid: 4.36 ± 0.83) and Gp 4 (Co: 5.39 ± 0.72, Mid: 4.46 ± 0.74). Gp 5 (Co: 9.04 ± 0.13, Mid: 8.14 ± 0.91) and Gp 6 (Co: 8.67 ± 0.42, Mid: 8.07 ± 0.07) exhibited higher EBS than Gp 3 and Gp 4 specimens. Adjunctive use of PDT for AH sealer (Gp 2) showed decreased EBS compared to Gp 1 specimens. EBS remained comparable for both MTA-Fillapex and Sealapex with and without PDT. CONCLUSION: AH Plus (Gp 1 and 2) and MTA-Fillapex (Gp 5 and 6) sealers displayed better EBS compared to Sealapex (Group 3 and 4). Adjunctive Application of PDT (CP) did not show a significant improvement in bond strength of sealers to root dentin.

Involvement of Muscarinic Receptors in Hypotensive and Diuretic Effects of Aqueous Soluble Fraction from Asphodelus tenuifolius Cav.

Background. Asphodelus tenuifolius Cav. (Asphodelaceae) is widely used in Pakistan traditional medicine as a hypotensive and diuretic agent. Despite the cardioprotective effects described for A. tenuifolius, the mechanisms involved in its probable hypotensive and diuretic effects have never been evaluated. Firstly, different extracts from A. tenuifolius seeds were obtained, and their antioxidant profiles and chemical constituents by LC-DAD-were determined, including molecular networking by the GNPS platform. Then, to evaluate changes in blood pressure, different groups of anesthetized normotensive rats were intravenously treated with the crude extract (AT-Cr, 1-50 mg/kg), aqueous (AS-AT, 1-25 mg/kg), n-butanol (BS-AT, 1-50 mg/kg), and dichloromethane fraction (DS-AT, 1-80 mg/kg). The diuretic effects of AT-Cr, AS-AT, BS-AT, and DS-AT at 100, 200, and 300 mg/kg, p.o. doses, were also evaluated in comparison with hydrochlorothiazide (HCTZ, 10 mg/kg, p.o). The urinary volume, sodium, potassium, and pH were estimated in the sample collected for 6 h from saline-loaded rats. Using pharmacological antagonists or inhibitors, we determine the involvement of acetylcholine, prostaglandins, and nitric oxide in A. tenuifolius-induced hypotensive and diuresis action. In addition, the activities of angiotensin-converting enzyme, erythrocyte carbonic anhydrase, and renal Na+/K+/ATPase were evaluated in vitro. Acute treatment with crude extract and fractions of A. tenuifolius exhibited significant hypotensive and diuretic potential in normotensive rats. However, AS-AT produced the most potent and significant dose-dependent hypotension and diuretic effects in normotensive rats. Previous treatment with atropine significantly reduced the hypotensive and diuretic action of AS-AT, but pretreatment with indomethacin or L-NAME did not affect these effects. Moreover, the 7-day treatment with AS-AT did not reduce activities of serum angiotensin-converting enzyme, erythrocyte carbonic anhydrase, and renal Na+/K+/ATPase. AS-AT showed four major compound node clusters, which included sugars, alkaloids, nucleoside, amino acid, and glycosylated flavonoids. This research supports and extends the traditional use of A. tenuifolius as a hypotensive and diuretic agent. The results showed that AS-AT from A. tenuifolius could present compounds responsible for hypotensive and diuretic activities through the activation of muscarinic receptors.

Phytochemical analysis of crude extract of Delphinium brunonianum and its effect on hypertension and metabolic perturbations in fructose fed rats.

The present study aims at phytochemical profiling and valuating the effect of crude extract of Delphinium brunonianum on fructose mediated rise in blood pressure and metabolic abnormalities in rats. Therefore, rats were fed on fructose (10%w/v) for 6 weeks. Rats in treatment groups received amlodipine 250, 500 and 1000 mg/kg of DB-Cr separately in concurrent to fructose. Various parameters of metabolic perturbations were assessed at the end of study. Further, DB-Cr was analyzed using LC-MS technique. DB-Cr exerted remarkable antihypertensive effect whereas, sympathetic hyperactivity and hyperinsulinemia in these rats was significantly blunted, further, endothelium functionality was successfully restored. LC-MS analysis of DB-Cr revealed the presence of a variety of chemical constituents (41) including quinic acid, scopolin, gingerol, Robinetin 3-rutinoside, KAPA and maleic acid. In conclusion, D. brunonianum possess the potential to combat the fructose mediated hypertension and metabolic perturbations, which may partially be due to its chemical constituents.

Repair strength and surface topography of lithium disilicate and hybrid resin ceramics with LLLT and photodynamic therapy in comparison to hydrofluoric acid.

AIM: The aim was to compare the repair bond strength and surface topography of lithium disilicate ceramics (LDC) and hybrid resin ceramics (HRC) using different surface conditioning treatments [low level laser therapy (LLLT), photodynamic therapy (PDT), hydrofluoric acid (HF) with silane and air abrasion (AA) and silane]. MATERIAL AND METHOD: Sixty specimens each of LDC and HRC were used. Discs were prepared for each group (6 × 2 mm), conditioned using different regimes. Specimens in group 1 and 5 were laser irradiated using Er,Cr:YSGG (ECYL), group 2 and 6 were conditioned using methylene blue photosensitizer (PDT), group 3 and 7 surface was treated with hydrofluoric acid and silane (HFA-S), group 4 and 8 conditioned with Al2O3 air abrasion and silane (AA-S). A Porcelain Repair Kit was used according to manufacturer recommendation in all samples. Peak universal bond adhesive was rubbed on ceramic surface and then bonded with composite resin. For shear bond strength testing the specimens were placed in a universal testing machine. A stereomicroscope at 40x magnification was used to analyse failure pattern. Five specimens in each group after surface treatment were evaluated for surface changes and topography using scanning electron microscopy. The mean repair bond strength was calculated using ANOVA and Tukey's post hoc test at a significance level of (p < 0.05). RESULT: The highest repair bond strength was observed in group 3 (LDC) (20.57 ± 3.58 MPa) (HFA-S), whereas, the lowest score was displayed in Group 2 (LDC) using methylene blue photosensitizer (MBPS) (12.18 ± 1.08 MPa). Similarly, in HRC the highest repair SBS was presented in group 8 (AA-S) (20.52 ± 2.51 MPa) and the lowest SBS values were exhibited by PDT treated group 6 (13.22 ± 0.62 MPa). CONCLUSION: A combination of mechanical and chemical surface treatments should be used in order to achieve adequate repair bond strength between resin composites and ceramic interface of LDC and HRC.

Antimicrobial efficacy of silver diamine fluoride in comparison to photodynamic therapy and chlorhexidine on canal disinfection and bond strength to radicular dentin.

AIM: To assess the antimicrobial efficacy of Silver diamine fluoride (SDF), Sodium hypochlorite (NaOCl), Chlorhexidine (CHX) and Photodynamic therapy (PDT) in radicular dentin and subsequent bond strength of Fiber reinforced composite post (FRCP) to radicular dentin. MATERIAL AND METHODS: Forty extracted premolars were disinfected and decoronated up-to cement-o-enamel junction (CEJ). Canals were instrumented using crown-down technique, dried and filled with gutta percha. Post space was prepared with the help of peso reamers and inoculated with E. faecalis. Samples were randomly allocated into four groups based on chemical irrigation (n = 10). Group 1, canal disinfected with (PDT, MB), group 2 (5.25 % NaOCl +17 % EDTA), group 3 (3.8 % SDF) and group 4 (CHX). Specimens were tested with confocal laser scanning microscopy analysis (CLSM) to confirm the presence of E. faecalis. Fibre post were luted in root canal and sectioned at three levels (apical, middle and coronal). Push-out test was performed and failure analysis of debonded surfaces was evaluated using stereomicroscope. Live and dead cells after exposure were calculated in percentage. One-way analysis of variance (ANOVA) assessed the means and standard deviations of extrusion bond strength (EBS). Tukey multiple comparison tests was used to compare means of bond strength (p = 0.05). RESULTS: The maximum antimicrobial efficacy against E. faecalis was observed in group 2 which included canal disinfected with 5.25 % NaOCl and 17 % EDTA (64.41 ±â€¯1.22). Similarly, CHX showed lowest effectiveness as canal irrigant against E.faecalis (58.29 ±â€¯2.41). Intragroup comparison revealed highest bond strength for group 2 at all three level. Similarly, the lowest EBS was found in CHX group. Inter-group comparison confirmed comparable EBS at all root levels (apical, middle and coronal) for group 2 and group 3 (p > 0.05). CONCLUSION: Radicular dentin treated with 5.25 % NaOCl and 17 % EDTA displayed highest antimicrobial and bond strength scores. SDF and PDT can potentially be used in canal irrigation.

Influence of Surface Conditioning on the Repair Strength of Bioactive Restorative Material.

OBJECTIVE: To evaluate the effects of surface treatment and repair material on the repair shear bond strength (SBS) of the bioactive restorative material. METHODS: A total of 240 Activa BioActive Restorative (Activa) discs were prepared, aged, and polished, and divided randomly into eight groups (n = 30). Groups 1-4 discs were repaired with bulk-fill flowable resin-based composite (Bulk-RBC), and Groups 5-8 discs with Activa. Surface treatment used for each repair material type were air abrasion with silica-coated 30-m Al2O3 particles (air abrasion) (Groups 2 & 6), Air abrasion with universal primer (Groups 3 & 7), and Air abrasion with universal adhesive (Groups 4 & 8). Groups 1 and 5 were controls without surface treatment. SBS test was performed, and the failure mode and surface topography were assessed. RESULTS: Surface treatment with air abrasion significantly improved the SBS for repair using both Activa and Bulk-RBC. Repair SBS using Activa was significantly higher compared with Bulk-RBC. Cohesive failure in substrate and mixed failures were most common in the surface-treated groups (2-4, 6-8). Air abrasion produced prominent surface topography changes compared with polishing. CONCLUSION: Air abrasion enhances the repair SBS of aged bioactive restorative material. The use of the same material (Activa) for repair affords a higher bond strength compared with the use bulk-RBC.

Treatment of caries affected dentin with different photosensitizers and its effect on adhesive bond integrity to resin composite.

OBJECTIVE: The aim of the present study was to evaluate the effect of different photosensitizers activated by photodynamic therapy (PDT) on shear bond strength (SBS) of composite resin on caries affected dentin compared to conventional cavity disinfectants. MATERIALS AND METHODS: Forty carious mandibular teeth were collected through non-traumatic extraction. All samples were embedded in polyvinyl cross-sections to the cemento-enamel junction. The preparation of caries-affected dentin was performed by grinding the specimens using silicon carbide discs. All specimens were arbitrarily allocated in to four groups (n = 10). Group 1, affected dentin disinfected with methylene blue (MB) [100 mg/L]; Group 2, caries affected dentin disinfected using curcumin photosensitizer (CP) [500 mg/L]; Group 3, affected dentin disinfected with indocyanin green photosensitizer (ICGP) [0.5 mL] Group 4, affected dentin treated with 3 % H2O2. Two-step self-etch adhesives was smeared on all specimens for 15 s and photopolymerized for 10 s. All specimens were placed in an incubator with humid environment for 24 h at 37 °C followed by positioning in universal testing machine for SBS testing. Failure mode examination of debonded samples was performed by a stereomicroscope. Analysis of variance (ANOVA) was used for inter group comparison through mean and standard deviation of each group. For multiple group comparison Tukey HSD was employed. Level of significance was established at p < 0.05. RESULT: In group 2 dentin disinfection using CP, displayed the highest SBS value (18.21 ± 1.39 MPa). Whereas, group 4 (3% H2O2 as cavity disinfectant) presented the lowest SBS values (13.39 ± 1.26 MPa). SBS in Group 3 (ICGP) (17.42 ± 0.55 MPa) specimens was comparable to group 2 (p > 0.05) samples. In group 1, samples disinfected with MB (15.18 ± 0.39 MPa) was found to be significantly lower compared to group 2 and group 3 (p < 0.05) respectively. CONCLUSION: Curcumin and Indocyanin green (ICGP) PS have the potential to be used as cavity disinfectant as it improves SBS of caries affected dentin to resin composite. Photodynamic therapy for disinfection of caries affected dentin will augment its bond strength, however its long-term effect on bond integrity still needs to be investigated.

Appraisal of disease-modifying potential of amlodipine as an anti-arthritic agent: new indication for an old drug.

Amlodipine, a second-generation calcium channel blocker, exhibits documented anti-inflammatory potential. Thereby, present investigation was accomplished with an aim to explore anti-arthritic potential of amlodipine, giving a second chance to an existing drug. For validation of anti-arthritic potential of amlodipine, some in vitro models comprised of bovine serum albumin- and egg albumin-induced protein denaturation along with membrane stabilization of red blood cell was being conducted. In vivo models comprised of formaldehyde-provoked acute arthritis and CFA-instigated chronic arthritic. Paw edema, arthritic index, body weight alterations, biochemical and hematological parameters, and ankle joint histological and radiographic investigations were appraised. Moreover, RT-PCR was conducted to evaluate the levels of several inflammatory markers. Molecular docking was being conducted targeting TNF-α, IL-1ß and IL-6 to establish the correlation between experimental and theoretical results. Amlodipine provides significant protection against denaturation being provoked by heating egg albumin and BSA along with stabilizing membrane of red blood cell, thereby proving in vitro anti-arthritic effect. A significant (p < 0.001) reduction in paw swelling was being observed with amlodipine in case of formaldehyde-instigated arthritis especially at the dose of 20 mg/kg. In case of CFA-provoked arthritis, reduction in paw volume and arthritic score while preservation of body weight loss and normal hematological and biochemical parameters in comparison to arthritic control were being manifested by amlodipine at the dose of 20 mg/kg. Gene expression level of TNF-α, IL-6 and IL-1ß was significantly reduced by amlodipine while an increase in expression level of IL-4 and IL-10 was evident in animals treated with piroxicam and amlodipine. Molecular docking analysis demonstrated strong binding interaction of amlodipine with TNF-α, IL-6 and IL-1ß thus providing a good correlation between experimental and theoretical results. Thus, current study is suggestive that amlodipine exhibits strong anti-arthritic potential and thus can be considered as a candidate for drug repurposing as anti-arthritic agent.

Influence of Er,Cr:YSGG Laser Dentin Conditioning on the Bond Strength of Bioactive and Conventional Bulk-Fill Dental Restorative Material.

Objective: The aim of the study was to evaluate shear bond strength (SBS) and modes of failure for bioactive (BA) material when bonded to Er,Cr:YSGG lased dentin in comparison with other conditioning regimes. Materials and methods: One hundred twenty extracted teeth were mounted and allocated into eight groups (n = 15) according to the surface conditioning [group 1: Er,Cr:YSGG laser + BA; group 2: Er,Cr:YSGG laser + multi-core (MC); group 3: Er,Cr:YSGG + Ketac Conditioner (polyacrylic acid) + BA; group 4: Er,Cr:YSGG + Ketac Conditioner + MC; group 5: conventional etch and rinse + BA (Control); group 6: conventional etch and rinse + MC (Control); group 7: self-etch + BA; group 8: self-etch + MC]. Based on the type of bulk-fill material, samples from groups 1, 3, 5, and 7 were bonded to BA and groups 2, 4, 6, and 8 were bonded to conventional MC bulk-fill material. All specimens were tested for SBS using universal testing machine. Ten samples from each group were assessed for modes of failure. Data were assessed using analysis of variance and Tukey multiple comparisons test. Results: The maximum SBS value was in group 6 (18.96 ± 0.315) with etch and rinse dentin conditioning and bonded to MC. The minimum SBS value was exhibited by group 7 (16.04 ± 0.854) self-etch conditioning bonded to BA. SBS among group 6 specimens were significantly higher than that of specimens in group 7 (p < 0.05). Among all groups the most common type of observed failure was adhesive. Conclusions: Dentin surface treatment with Er,Cr:YSGG phototherapy for the adhesive bonding of BA and conventional bulk-fill resin composite MC showed comparable bond strength outcomes to conventional conditioning techniques. Er,Cr:YSGG laser dentin conditioning of dentin for bonding of bulk-fill BA materials has potential to be used clinically as it shows potential for adequate adhesive bond integrity.

Effect of photodynamic therapy and ErCrYSGG laser irradiation on the push-out bond strength between fiber post and root dentin.

BACKGROUND: To evaluate the push out bond strength and modes of failure of fiber post by using photodynamic therapy (PDT), Er,Cr:YSGG laser and conventional cleaning and shaping (CCS). METHODS: Sixty maxillary anterior teeth were sectioned horizontaly 2 mm incisal to the cemento-enamel junction, and root canal were prepared for post space. Tapered fiber posts were placed inside the root canal after post space was made. The fiber posts were subjected to PDT, Er,Cr:YSGG laser and CSS with 20 specimens in each group. The specimens obtained were sectioned in cervical and apical sections. A universal testing machine was used to perform the push out test and the push out bond strength was formulated by σ = C/A, expressed in mega-pascals (MPa). RESULTS: The highest mean push out bond strength was achieved by PDT group (8.08 ±â€¯2.73 MPa) and the lowest was shown by specimens in CCS group (7.45 ±â€¯1.04 MPa). ANOVA showed no statistical difference among the experimental groups (p = 0.481). In the cervical segments, the mean push-out bond strength was found to be slightly higher for all three groups compared to apical segments (P < 0.05). The independent t-tests results showed that the mean push-out bond strength values of the cervical segments were slightly higher than the apical segments in PDT, Er,Cr:YSGG and CSS groups (P < 0.05). Significant differences were observed when mean push-out bond strengths were compared for both cervical (p = 0.037) and apical (p = 0.019) segments between all the groups. Twenty-one failures were found at the interface between the adhesive and the dentin surface, 6 failures were observed at the interface between the adhesive and post, whereas 5 failures were mixed. CONCLUSION: Push-out bond strength to root canal dentin were not affected by Er,Cr:YSGG compared with conventional cleaning and shaping. However, PDT produced the smallest number of failure modes and slightly higher push-out bond strength to root dentin.

Effect of Er,Cr:YSGG on bond strength and microleakage of dentin bonded to resin composite with different distance and irradiation time.

BACKGROUND: To explore the surface modification of dentin using Er,Cr:YSGG phototherapy and bonding system on the shear bond strength and microleakage scores at two different distances and times of laser irradiation. MATERIALS AND METHODS: Ninety disease-free third molars were collected for the experiment. The four laser groups were divided on the basis of non-contact mode distance and time of irradiation: Group-I: Distance of 1 mm with duration of 60 s; Group-II: Distance of 1 mm with duration of 120 s; Group-III: Distance of 2 mm with duration of 60 s and; Group-IV: Distance of 2 mm with duration of 120 s. The surface for control group (Group-EB: Etch & bond group) was treated with etch and rinse bonding regime. Specimens from all the groups were assessed for shear bond strength and microleakage scores. Samples from all groups were immersed in 2% methylene blue for 24 h and assessed under a digital microscope for microleakage. RESULTS: The lowest bond strength and microleakage was observed in the control group EB. The maximum bond strength score was observed in Group-IV with mean scores 23.41 ± 1.83, while the maximum microleakage scores were observed in Group-II and Group-IV with mean scores 1.0 ± 0.36 and 1.0 ± 0.11, respectively. The lowest bond strength and microleakage among laser groups was observed in Group-II and Group-III, respectively. However, specimens in laser groups showed comparable bond strength and microleakge scores (p > 0.05). For bond strength and microleakage values, analysis of variance showed significant difference among the study groups (p < 0.001). Using Tukey post hoc test, bond strength and microleakage of laser groups showed statistically significant values than EB group. CONCLUSION: Use of phototherapy using Er,Cr:YSGG for dentin surface treatment has the potential for clinical application in comparison to conventional conditioning technique. Increasing the distance of laser application and time of irradiation has significant effect on the shear bond strength and microleakage scores of dentin bonded to resin composite. However, further in-vitro analysis should be undertaken in order to prove such findings.

Efficacy of phototherapy with different conventional surface treatments on adhesive quality of lithium disilicate ceramics.

AIM: The aim of the present study was to evaluate shear bond strength (SBS) of LDC and resin composite in combination with phototherapy and different ceramic surface treatments. MATERIALS AND METHOD: Forty Lithium Disilicate Glass Ceramic (LDC) disks measuring (4 × 4 x 9 mm) were fabricated. The specimens were randomly divided into four groups (n = 10 each) according to the surface treatment. Group 1 H F + Silane (Control); Group 2 HF + Ultrasonic bath + Silane; Group 3 SECP (Self etch ceramic primer) and Group 4 phototherapy (Er, Cr: YSGG) + Silane. On each ceramic disk a resin build-up was done. For SBS the specimens were subjected to increasing load with a transversal velocity of 1 ml/min on a universal testing machine on a ceramic resin interface. Failure mode was evaluated using digital microscope. The failure modes were divided into adhesive, cohesive and admixed interface. Data through bond strength testing was tabulated using statistical program for social science (SPSS). Means and standard deviations were compared using analysis of variance and Tukey's post hoc test (p < 0.05). RESULTS: The maximum and minimum bond strength was observed in group 2 [19.58(1.011)] and group 1 [17.14(1.122)] respectively. The bond strength among experimental group 1 [17.14 (1.122)] and group 4 [17.48(1.145)] were found to be comparable. Specimens in Group 2 displayed significantly higher bond strength among all experimental groups. Commonly adhesive failure mode was observed in the present study, with an incidence of 60%, 100% and 70% in groups 1,2 and 3 correspondingly. CONCLUSION: Phototherapy using laser at frequency 30 Hz and 4.5 W can be used as a surface conditioner for LDC alternate to HF acid. Conditioning of LDC using Self-etch ceramic primer showed better SBS outcomes as compared to phototherapy (Er, Cr: YSGG laser).

Effect of phototherapy on dentin bond strength and microleakage when bonded to resin with different conditioning regimes.

AIM: To assess the efficacy of phototherapy on bond strength and microleakage of bulk fill composites in comparison to conventional dentin surface treatments. MATERIALS AND METHODS: Ninety human third molars were divided into 3 groups (n = 30 each) according to surface conditioning treatment. Group 1 (Etch and Bond) EB, group 2 was treated with Er,Cr: YSGG (ECL) and group 3 was treated with a diode laser (DL). Based on the type of bulk fill resin composite, samples were divided into six sub-groups. In sub-group 1, 2 and 3 (n = 15) build-up was done using ZirconCore (ZC) and these subgroups were named as EB-ZC, ECL-ZC and DL-ZC. In subgroup 4,5,6 (n = 15) MulticCore Flow (MC) was used for core build-up and these subgroups were named as EB-MC, ECL-MC, DL-MC. For shear bond strength (SBS) all the samples were exposed to loads using a universal testing machine. Five samples each from all subgroups were immersed in methylene blue dye for 24 h prior to microleakage testing. Data were assessed using analysis of variance and Tukey multiple comparisons test. RESULTS: Among all the groups the lowest bond strength was achieved in DL-ZC [10.45(0.459)]. Similarly, the highest bond strength was attained in EB-MC[17.84(0.925)]. The highest microleakage scores amongst different group was exhibited in DL-ZC [82.45(39.459)] whereas, the lowest microleakage scores were displayed in EB-MC [31.21(15.92)]. CONCLUSION: Phototherapy in the form of Er,Cr:YSGG laser showed comparable adhesive bond outcomes to conventional etch and bond dentin conditioning techniques. Moreover, Multicore bulk fill material showed better bond strength and microleakage scores than zirconium particle infiltrated bulk fill composite in the presence of dentin phototherapy.