Co-inoculation of phosphate-solubilizing bacteria and phosphate accumulating bacteria in phosphorus-enriched composting regulates phosphorus transformation by facilitating polyphosphate formation.

This study aimed to explore the impact of co-inoculating phosphate-solubilizing bacteria (PSB) and phosphate accumulating bacteria (PAB) on phosphorus forms transformation, microbial biomass phosphorus (MBP) and polyphosphate (Poly-P) accumulation, bacterial community composition in composting, using high throughput sequencing, PICRUSt 2, network analysis, structural equation model (SEM) and random forest (RF) analysis. The results demonstrated PSB-PAB co-inoculation (T1) reduced Olsen-P content (1.4 g) but had higher levels of MBP (74.2 mg/kg) and Poly-P (419 A.U.) compared to PSB-only (T0). The mantel test revealed a significantly positive correlation between bacterial diversity and both bioavailable P and MBP. Halocella was identified as a key genus related to Poly-P synthesis by network analysis. SEM and RF analysis showed that pH and bacterial community had the most influence on Poly-P synthesis, and PICRUSt 2 analysis revealed inoculation of PAB increased ppk gene abundance in T1. Thus, PSB-PAB co-inoculation provides a new idea for phosphorus management.

Carbon-containing additives changes the phosphorus flow by affecting humification and bacterial community during composting.

Phosphorus recycling from organic wastes to prepare a fertilizer by composting is promising. The aim of this study was to compare the effect of diverse carbon-containing additives (T1, glucose; T2, biochar; T3, woody peat) on phosphorus (P) fractions transformations, humus formation and bacterial community succession in chicken manure composting. Results showed that orthophosphate monoester was significantly related to the humification process, and glucose or woody peat addition increased the P in humus. Lentibacillus was a key carbon cycle bacteria related to organics stabilization affected by carbon-containing additives. Redundancy analysis and variation partitioning indicated that phosphatase enzyme activity driven by bacterial community and humic substance had 59.7% contribution to P fractions dynamics. The findings highlight an efficient humus-regulation P stabilization way, notably in composting adding glucose to form humus with a better binding ability to labile P forms and phosphatase.

Phosphorus transformation behavior and phosphorus cycling genes expression in food waste composting with hydroxyapatite enhanced by phosphate-solubilizing bacteria.

This study aimed to explore the effect of phosphate-solubilizing bacteria (PSB) Bacillus inoculation in the cooling stage on hydroxyapatite dissolution, phosphorus (P) forms transformation, and bacterial P cycling genes in food waste composting with hydroxyapatite. Results indicated that PSB inoculation promoted the dissolution of hydroxyapatite, increased P availability of compost by 8.1% and decreased the ratio of organic P to inorganic P by 10.2% based on sequential fractionation and 31P nuclear magnetic resonance spectroscopy. Illumina sequencing indicated Bacillus relative abundance after inoculation increased up to one time higher than control after the cooling stage. Network analysis and metabolic function of bacterial community analysis suggested inorganic P solubilizing genes of Bacillus and organic P mineralization genes of other genera were improved after inoculation in the core module. Therefore, bioaugmentation of PSB in the cooling stage may be a potential way to improve P bioavailability of bone and food waste in composting.

Soil organic carbon stability mediate soil phosphorus in greenhouse vegetable soil by shifting phoD-harboring bacterial communities and keystone taxa.

Addition of organic amendments, such as manure and straw, to arable fields as a partial substitute for mineral phosphorus (P), are a sustainable practice in high-efficiency agricultural production. Different organic inputs may induce varied soil organic carbon (OC) stability and phoD harboring microbes, subsequently regulate P behavior, but the underlying mechanisms are poorly understood. A 11-year field experiment examined P forms by 31P-nuclear magnetic resonance (NMR), OC chemical composition by 13C NMR, and biologically-based P availability methods, phoD bacterial communities, and their co-occurrence in soils amended with chemical P fertilizer (CF), chemical P partly substituted by organic amendments including pig manure (CM), a mixture of pig manure and corn straw (CMS), and corn straw (CS), with equal P input in all treatments. Organic amendments significantly increased soil labile Pi (CaCl2-P, citrate-P, 2.91-3.26 and 1.16-1.32 times higher than CF) and Po (enzyme-P, diesters, 4.08-7.47 and 1.71-2.14 times higher than CF) contents and phosphatase activities, while significantly decreased aromaticity (AI) and recalcitrance indexes (RI) of soil C, compared with CF. The keystone genera in manured soils (Alienimomas and Streptomyces) and straw-applied soils (Janthinobacterium and Caulobacter) were significantly correlated with soil enzyme-P, microbial biomass P (MBP), diesters, and citrate-P. Soil AI and RI were significantly correlated with the phoD keystone and soil P species. It suggested that the keystone was impacted by soil OC stability and play a role in regulating P redistribution in amended soils. This study highlights how manure and straw incorporation altered soil OC stability, shaped the phoD harboring community, and enhanced soil P biological processes promoted by the keystone taxa. The partial substitution of mineral P by mixture of manure and straw is effectively promote soil P availability and beneficial for environmental sustainability.

Humic acid and phosphorus fractions transformation regulated by carbon-based materials in composting steered its potential for phosphorus mobilization in soil.

This study investigated the effects of different carbon-based additives including biochar, woody peat, and glucose on humic acid, fulvic acid, and phosphorus fractions in chicken manure composting and its potential for phosphorus mobilization in soil. The results showed that the addition of glucose effectively increased the total humic substance content (90.2 mg/g) of composts, and the fulvic acid content was significantly higher than other groups (P < 0.05). The addition of biochar could effectively improve the content of available phosphorus by 59.9% in composting. The addition of carbon-based materials to the composting was beneficial for the production of more stable inorganic phosphorus in the phosphorus fraction. The highest proportion of soluble inorganic phosphorus components of sodium hydroxide was found in group with woody peat addition (8.7%) and the highest proportion of soluble inorganic phosphorus components of hydrochloric acid was found in group with glucose addition (35.2%). The compost products with the addition of biochar (humic acid decreased by 17.9%) and woody peat (fulvic acid decreased by 72.6%) significantly increased soil humic acid mineralization. The compost products with the addition of biochar was suitable as active phosphate fertilizer, while the compost products with the addition of glucose was suitable as slow-release phosphate fertilizer.

Influence of carbon-to-phosphorus ratios on phosphorus fractions transformation and bacterial community succession in phosphorus-enriched composting.

This study aims to assess the effect of different carbon-to-phosphorus (C:P) ratios on phosphorus (P) fractions transformation, bacterial community succession and microbial P-solubilizing function in kitchen waste composting with rock phosphate (RP) amendment and phosphate-solubilizing bacteria (PSB) inoculation. Results indicated that initial C:P ratio at 50 enhanced organic carbon degradation, available P (AP) accumulation, the amount of PSB and pqqC gene abundance in composting but higher C:P ratio increased microbial biomass phosphorus (MBP) content. Redundancy analysis showed C:P ratios, PSB amount and pqqC gene abundance greatly affected bacterial community diversity and composition. Network analysis indicated that lower C:P ratio enhanced the interaction frequency in core bacterial network for AP transformation. Variance partitioning analysis abiotic factors contributed more to MBP and AP conversion. The study revealed that C:P ratio could directly drive PSB to regulate P fractions and the accumulation of MBP or AP in P-enriched composting.

Lymph-Node-Targeted Cholesterolized TLR7 Agonist Liposomes Provoke a Safe and Durable Antitumor Response.

Toll-like receptor (TLR) agonists as the potent stimulants of an innate immune system hold promises for applications in anticancer immunotherapy. However, most of them are limited in the clinical translation due to the uncontrolled systemic inflammatory response. In the current study, 1V209, a small molecule TLR7 agonist, was conjugated with cholesterol (1V209-Cho) and prepared into liposomes (1V209-Cho-Lip). 1V209-Cho-Lip exerted minimal toxic effects and enhanced the transportation ability in lymph nodes (LNs) compared with 1V209. 1V209-Cho-Lip treatment inhibited tumor progression in CT26 colorectal cancer, 4T1 breast cancer, and Pan02 pancreatic ductal cancer models through inducing effective DC activation and eliciting CD8+ T cell responses. Furthermore, 1V209-Cho-Lip induced tumor-specific memory immunity to inhibit cancer recurrence and metastasis. These results indicate that cholesterol conjugation with 1V209 is an effective approach to target lymph nodes and to reduce the adverse effects. This work provides a rational basis for the distribution optimization of TLR agonists for potential clinical use.

Inoculation of phosphate-solubilizing bacteria (Bacillus) regulates microbial interaction to improve phosphorus fractions mobilization during kitchen waste composting.

Bacillus presents in most composts as core microbial taxa and is widely used as inoculant in composting. However, the role of Bacillus as phosphate-solubilizing bacteria (PSB) inoculant in composting and the response of indigenous bacterial community are unclear. This study used redundancy analysis (RDA), network analysis and structural equation model (SEM) to investigate the dynamics of phosphorus (P) fractions, bacterial community, and microbial interaction in composting with PSB (Bacillus sp. P6) inoculation. Results indicated that Bacillus inoculation increased Olsen P content, organic matter degradation, and bacterial diversity, benefiting P fractions mobilization during composting. RDA showed that pH was the main factor influencing P fractions transformation and bacterial taxa. Network analysis and SEM revealed that Bacillus indirectly improved the contribution of bacterial community on P mobilization by enhancing microbial interactions. Therefore, Bacillus with P solubilizing function may be a potential inoculant to regulate the biotic process of P transformation.

Phosphorus excess changes rock phosphate solubilization level and bacterial community mediating phosphorus fractions mobilization during composting.

This study investigated the changes of phosphorus (P) fractions, bacterial community and their response to available P or carbon (C):P during composting with different rock phosphate (RP) addition levels. Results showed that adding RP at 10% or 15% promoted the rise of temperature, maturity and Olsen P accumulation in composting, which had a higher amount of RP solubilization than other groups. Available P changed bacterial composition and decreased diversity in composts. RP solubilization efficiency was negatively correlated to C:P ratio and the highest (22.7%) when 10% RP was added, in which bacterial community changed from "function redundancy" to "intensive P-solubilization". Low C:P ratio (〈300) increased the RP solubilization ratio especially within 135-160. Therefore, this study proposed that adding P-rich substrates to decrease C:P ratio could regulate P-solubilizers' activity for increasing RP solubilization efficiency during composting.

Autophagy-based unconventional secretion of HMGB1 by keratinocytes plays a pivotal role in psoriatic skin inflammation.

The precise mechanism through which macroautophagy/autophagy affects psoriasis is poorly understood. Here, we found that keratinocyte (KC) autophagy, which was positively correlated with psoriatic severity in patients and mouse models and could be inhibited by mitogen-activated protein kinase (MAPK) family inactivation. The impairment of autophagic flux alleviated psoriasisform inflammation. We also found that an autophagy-based unconventional secretory pathway (autosecretion) dependent on ATG5 (autophagy related 5) and GORASP2 (golgi reassembly stacking protein 2) promoted psoriasiform KC inflammation. Moreover, the alarmin HMGB1 (high mobility group box 1) was more effective than other autosecretory proteins in regulating psoriasiform cutaneous inflammation. HMGB1 neutralization in autophagy-efficient KCs eliminated the differences in psoriasiform inflammation between Krt14+/+-Atg5f/f KCs and Krt14Cre/+-atg5f/f KCs, and conversely, recombinant HMGB1 almost completely restored psoriasiform inflammation in Krt14Cre/+-atg5f/f KCs in vivo. These results suggest that HMGB1-associated autosecretion plays a pivotal role in cutaneous inflammation. Finally, we demonstrated that Krt14Cre/+-hmgb1f/f mice displayed attenuated psoriatic inflammation due to the essential crosstalk between KC-specific HMGB1-associated autosecretion and γδT cells. Thus, this study uncovered a novel autophagy mechanism in psoriasis pathogenesis, and the findings imply the clinical significance of investigating and treating psoriasis.Abbreviations: 3-MA: 3-methyladenine; ACTB: actin beta; AGER: advanced glycosylation end-product specific receptor; Anti-HMGB1: anti-HMGB1 neutralizing antibody; Anti-IL18: anti-IL18 neutralizing antibody; Anti-IL1B: anti-IL1B neutralizing antibody; ATG5: autophagy related 5; BAF: bafilomycin A1; BECN1: beclin 1; CASP1: caspase 1; CCL: C-C motif chemokine ligand; CsA: cyclosporine A; ctrl shRNA: lentivirus harboring shRNA against control; CXCL: C-X-C motif chemokine ligand; DCs: dendritic cells; DMEM: dulbecco's modified Eagle's medium; ELISA: enzyme-linked immunosorbent assay; EM: electron microscopy; FBS: fetal bovine serum; GORASP2 shRNA: lentivirus harboring shRNA against GORASP2; GORASP2/GRASP55: golgi reassembly stacking protein 2; GR1: a composite epitope between LY6 (lymphocyte antigen 6 complex) locus C1 and LY6 locus G6D antigens; H&E: hematoxylin and eosin; HMGB1: high mobility group box 1; HMGB1 shRNA: lentivirus harboring shRNA against HMGB1; IFNG/IFN-γ: interferon gamma; IL17A: interleukin 17A; IL18: interleukin 18; IL1A/IL-1α: interleukin 1 alpha; IL1B/IL-1ß: interleukin 1 beta; IL22/IL-22: interleukin 22; IL23A: interleukin 23 subunit alpha; IL23R: interleukin 23 receptor; IMQ: imiquimod; ITGAM/CD11B: integrin subunit alpha M; ITGAX/CD11C: integrin subunit alpha X; IVL: involucrin; KC: keratinocyte; KD: knockdown; KO: knockout; Krt14+/+-Atg5f/f mice: mice bearing an Atg5 flox allele, in which exon 3 of the Atg5 gene is flanked by two loxP sites; Krt14+/+-Hmgb1f/f: mice bearing an Hmgb1 flox allele, in which exon 2 to 4 of the Hmgb1 gene is flanked by two loxP sites; Krt14Cre/+-atg5f/f mice: keratinocyte-specific atg5 knockout mice generated by mating Atg5-floxed mice with mice expressing Cre recombinase under the control of the promoter of Krt4; Krt14Cre/+-hmgb1f/f mice: keratinocyte-specific hmgb1 knockout mice generated by mating Hmgb1-floxed mice with mice expressing Cre recombinase under the control of the promoter of Krt14; Krt14-Vegfa mice: mice expressing 164-amino acid Vegfa splice variant recombinase under the control of promoter of Krt14; LAMP1: lysosomal associated membrane protein 1; LDH: lactate dehydrogenase; LORICRIN: loricrin cornified envelope precursor protein; M5: TNF, IL1A, IL17A, IL22 and OSM in combination; MAP1LC3/LC3: microtubule associated protein 1 light chain 3; MAPK: mitogen-activated protein kinase; MKI67: marker of proliferation Ki-67; MTT: thiazolyl blue tetrazolium bromide; NFKB/NF-κB: nuclear factor kappa B; NHEKs: primary normal human epidermal keratinocytes; NS: not significant; OSM: oncostatin M; PASI: psoriasis area and severity index; PtdIns3K: class III phosphatidylinositol 3-kinase; qRT-PCR: quantitative RT-PCR; RELA/p65: RELA proto-oncogene, NF-kB subunit; rHMGB1: recombinant HMGB1; rIL18: recombinant interleukin 18; rIL1B: recombinant interleukin 1 beta; S100A: S100 calcium binding protein A; SQSTM1/p62: sequestosome 1; T17: IL17A-producing T; TCR: T-cell receptor; tcrd KO mice: tcrd (T cell receptor delta chain) knockout mice, which show deficient receptor expression in all adult lymphoid and epithelial organs; TLR: toll-like receptor; TNF/TNF-α: tumor necrosis factor; WOR: wortmannin; WT: wild-type; γδT17 cells: IL17A-producing γδ T cells.

Composting with biochar or woody peat addition reduces phosphorus bioavailability.

Biochar and woody peat have been recognized as an additive to reduce carbon and nitrogen loss during composting. Yet little is known about their influences on the transformation of phosphorus (P) fractions in composting. This study investigated the quantitative and qualitative changes in different P forms during composting with adding biochar or woody peat using sequential extraction and P K-edge X-ray absorption near-edge structure (XANES). The results showed that compost products from the treatment with adding woody peat had a higher HA/FA (the ratio of humic acid to fulvic acid) compared to biochar treatment and the control, suggesting that the addition of woody peat might benefit the humification process of composting. Sequential extraction and XANES illustrated that adding biochar or woody peat limited the P availability. Biochar increased the proportion of Pi and woody peat decreased the conversion from Po to Pi compared to the control. Structural equation modeling and redundancy analysis suggested that biochar improved the refractory P based on the indirect effects of NH4+-N by regulating microbial community, while woody peat was beneficial for Po accumulation by affecting humic acid. Taken together, this research provides basis for regulating the nutrient level of carbon, nitrogen, and phosphorus in composts and reducing environmental risks.

History of petroleum disturbance triggering the depth-resolved assembly process of microbial communities in the vadose zone.

Biogeochemical gradient forms in vadose zone, yet little is known about the assembly processes of microbial communities in this zone under petroleum disturbance. This study collected vadose zone soils at three sites with 0, 5, and 30 years of petroleum contamination to unravel the vertical microbial community successions and their assembly mechanisms. The results showed that petroleum hydrocarbons exhibited higher concentrations at the long-term contaminated site, showing negative impacts on some soil properties, retarding in the surface soils and decreasing along soil depth. Cultivable fraction of heterotrophic bacteria and microbial α-diversity decreased along depth in vadose zones with short-term/no contamination history, but exhibited an opposite trend with long-term contamination history. Petroleum contamination intensified the vertical heterogeneity of microbial communities based on the contamination time. Microbial co-occurrence network revealed the lowest species co-occurrence pattern at the long-term contaminated site. The distance-decay patterns and null model analysis together suggested distinct assembly mechanisms at three sites, where dispersal limitation (42-45%) was higher and variable and homogenizing selections were lower (37-38%) in vadose zones under petroleum disturbance than those in the uncontaminated vadose zone. Our findings help to better understand the subsurface biogeochemical cycles and bioremediation of petroleum-contaminated vadose zones.

cGAS-STING pathway in cancer biotherapy.

The activation of the cGAS-STING pathway has tremendous potential to improve anti-tumor immunity by generating type I interferons. In recent decades, we have witnessed that producing dsDNA upon various stimuli is an initiative factor, triggering the cGAS-SING pathway for a defensive host. The understanding of both intracellular cascade reaction and the changes of molecular components gains insight into type I IFNs and adaptive immunity. Based on the immunological study, the STING-cGAS pathway is coupled to cancer biotherapy. The most challenging problem is the limited therapeutic effect. Therefore, people view 5, 6-dimethylxanthenone-4-acetic acid, cyclic dinucleotides and various derivative as cGAS-STING pathway agonists. Even so, these agonists have flaws in decreasing biotherapeutic efficacy. Subsequently, we exploited agonist delivery systems (nanocarriers, microparticles and hydrogels). The article will discuss the activation of the cGAS-STING pathway and underlying mechanisms, with an introduction of cGAS-STING agonists, related clinical trials and agonist delivery systems.

Therapeutic Effect and Mechanisms of the Novel Monosulfactam 0073.

This study aimed to evaluate the antimicrobial activity of the novel monosulfactam 0073 against multidrug-resistant Gram-negative bacteria in vitro and in vivo and to characterize the mechanisms underlying 0073 activity. The in vitro activities of 0073, aztreonam, and the combination with avibactam were assessed by MIC and time-kill assays. The safety of 0073 was evaluated using 3-(4,5-dimethylthizol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and acute toxicity assays. Murine thigh infection and pneumonia models were employed to define in vivo efficacy. A penicillin-binding protein (PBP) competition assay and confocal microscopy were conducted. The inhibitory action of 0073 against ß-lactamases was evaluated by the half-maximal inhibitory concentration (IC50), and resistance development was evaluated via serial passage. The monosulfactam 0073 showed promising antimicrobial activity against Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumannii isolates producing metallo-ß-lactamases (MBLs) and serine ß-lactamases. In preliminary experiments, compound 0073 exhibited safety both in vitro and in vivo In the murine thigh infection model and the pneumonia models in which infection was induced by P. aeruginosa and Klebsiella pneumoniae, 0073 significantly reduced the bacterial burden. Compound 0073 targeted several PBPs and exerted inhibitory effects against some serine ß-lactamases. Finally, 0073 showed a reduced propensity for resistance selection compared with that of aztreonam. The novel monosulfactam 0073 exhibited increased activity against ß-lactamase-producing Gram-negative organisms compared with the activity of aztreonam and showed good safety profiles both in vitro and in vivo The underlying mechanisms may be attributed to the affinity of 0073 for several PBPs and its inhibitory activity against some serine ß-lactamases. These data indicate that 0073 represents a potential treatment for infections caused by ß-lactamase-producing multidrug-resistant bacteria.

Biogas slurry as an activator for the remediation of petroleum contaminated soils through composting mediated by humic acid.

Soil pollution caused by petroleum hydrocarbons is a widespread environmental problem. Composting is one of the cost-effective solutions for petroleum hydrocarbons removal but limited by low efficiency of bioremediation, leading to high phytotoxicity. Given that biogas slurry as nutrients can alter the microbial activity, the aim of this study was to investigate the role of biogas slurry on the remediation of petroleum contaminated soils in composting. Herein, we added biogas slurry into the composting of hydrocarbon contaminated soil to investigate its effect on the biodegradation of petroleum hydrocarbons, humic acid (HA) transformation and the safety of product. The results showed that biogas slurry addition improved the degradation of organic matter and total petroleum hydrocarbons (TPH) (especially C > 16), but also increased 18.0% of germination index and the humification degree of HA. The estrone from biogas slurry was removed during composting and did not affect the phytotoxicity level of compost. Redundancy analysis and structural equation modeling indicated that TPH degradation was significantly related to the humification of HA components and total nitrogen from biogas slurry, which contributed to composting safety. Therefore, biogas slurry could be a possible activator for the remediation of petroleum contaminated soils through composting mediated by HA transformation, which is beneficial to obtain the composts with a lower phytotoxicity and higher maturity for soil application.

Indirect effect of nutrient accumulation intensified toxicity risk of metals in sediments from urban river network.

The levels of metals in sediments of urban river ecosystems are crucial for aquatic environmental health and pollution assessment. Yet little is known about the interaction of nutrients with metals for environmental risks under contamination accumulation. Here, we combined hierarchical cluster, correlation, and principal component analysis with structural equation model (SEM) to investigate the pollution level, source, toxicity risk, and interaction associated with metals and nutrients in the sediments of a river network in a city area of East China. The results showed that the pollution associated with metals in sediments was rated as moderate degree of contamination load and medium-high toxicity risk in the middle and downstream of urban rivers based on contamination factor, pollution load index, and environmental toxicity quotient. The concentration of mercury (Hg) and zinc (Zn) showed a significant correlation with toxic risks, which had more contribution to toxicity than other metals in the study area. Organic nitrogen and organic pollution index showed heavily polluted sediments in south of the study area. Though correlation analysis indicated that nutrients and metals had different input zones from anthropogenic sources in the urban river network, SEM suggested that nutrient accumulation indirectly intensified toxicity risk of metals by 13.6% in sediments. Therefore, we suggested the combined consideration of metal toxicity risk with nutrient accumulation, which may provide a comprehensive understanding to identify sediment pollution. Graphical abstract Toxicity rate of metals in sediments from urban river network indirectly intensified by nutrients accumulation.

Reducing ammonia and greenhouse gas emission with adding high levels of superphosphate fertilizer during composting.

Previous studies revealed that superphosphate fertilizer (SSP) as an additive in compost can reduce the nitrogen loss and improve the effectiveness of phosphorus during composting. However, few studies have explored the influence of adding SSP with high levels on ammonia and greenhouse gas emission and the suitable amount for SSP addition according to a combined assessment of the composting process and product. The present study aimed to evaluate the impact of SSP with high additive amounts on NH3, CO2, CH4, and N2O emission and organic carbon loss. All piles were mixtures of pig manure and cornstalks with different levels of SSP addition including 10%, 14%, 18%, 22%, 26%, and 30% dry weight basis of raw materials. Compared with the control without SSP, the amount of NH3 cumulative emissions was decreased by 23.8-48.1% for the treatments with 10-30% SSP addition, and the emission of greenhouse gas including CO2, CH4, and N2O by 20.9-35.5% (CO2 equivalent) was reduced by 20.9-35.5%. Adding SSP with the amount exceeding 14% to compost could reduce CO2 emissions by 32.0-38.4% and more than 30% carbon loss at the end of composting but exceeding 26% had an adverse impact on maturity of the composts. Therefore, considering the maturity and safety of compost and gas emission reduction, 14-26% SSP was the optimum amount for composting addition, which is an effective and economical way to increase the nutrient level of carbon, nitrogen, and phosphorus in compost and reduce environmental risks.

Preclinical Evaluation of Chimeric Antigen Receptor-Modified T Cells Specific to Epithelial Cell Adhesion Molecule for Treating Colorectal Cancer.

Chimeric antigen receptor-modified T cells (CAR-T cells) have emerged as a promising cancer immunotherapy for solid tumors. Epithelial cell adhesion molecule (EpCAM) is overexpressed in a variety of tumors and is recognized as a biomarker for circulating tumor cells and cancer stem cells, representing an attractive target for adoptive T-cell immunotherapy. This study generated third-generation CAR-T cells with redirected specificity to EpCAM (EpCAM CAR-T) by lentiviral vector. The study demonstrated that EpCAM CAR-T cells can elicit lytic cytotoxicity to target cells in an EpCAM-dependent manner and secrete cytotoxic cytokines, including interferon gamma and tumor necrosis factor alpha. Furthermore, adoptive transfer of EpCAM CAR-T cells significantly delayed tumor growth and formation in xenograft models. In addition, the safety evaluation showed that CAR-T cells have no systemic toxicity in mice. The data confirmed the antitumor ability and safety of CAR-T cells targeting EpCAM and may provide a new target for CAR-T cell therapies in treating solid tumors.

Three-Dimensional Ameliorated Biologics Elicit Thymic Renewal in Tumor-Bearing Hosts.

Cancer-initiating/sustaining stem cell subsets (CSCs) have the potential to regenerate cancer cell populations and are resistant to routine therapeutic strategies, thus attracting much attention in anticancer research. In this study, an innovative framework of endogenous microenvironment-renewal for addressing such a dilemma has been just developed. CSCs in three-dimensional multipotent spheroid-engineered biologics were prepared with 150 Gy radiation and inoculated into 15-mo-old BALB/c and C57BL/6 mice bearing diverse advanced tumors covering Mammary 4T1, liver Hepa, lung LL/2, and colon C26 tumors and distant metastases. Subsequently, the systematic microenvironment of tumor-bearing hosts was rapidly remodeled to resettle thymic cortex and medulla rudiment as an endogenous foxn1-thymosin reprogramming TCR-repertoire for resetting MHC-unrestricted multifunction renewal. Postrenewal Vγ4γδT-subsets would bind and lead migrating CSCs into apoptosis. Moreover, TCR repertoire multifunction renewal could reverse tumor metastases from tumoricidal resistance into eventual regression as a blockade of cancer-sustaining Bmi-1/Nanog-Oct4-Sox2 renewal loop with sequent multivalent depletion of both migrating/in situ CSCs and non-stem terminal cancer cell subsets. This study represents a promising start to set up a generalizable strategy of three-dimensional biologics evoking an endogenous integral microenvironment into pluripotent renewal versus advanced cancer.

Organophosphorus-degrading bacterial community during composting from different sources and their roles in phosphorus transformation.

The goals of this study were to identify the key culturable organophosphorus-degrading bacteria (OPDB) that contributed to regulating different phosphorus (P) fractions and evaluate the roles of OPDB and inorganic phosphate-solubilizing bacteria (IPSB) in P transformation during different composting. The results showed that the amounts, incidence and community composition of OPDB for composts from diverse sources were distinctly different but significantly related to temperature and organic matter content. Fifteen key OPDB correlated closely with different P fractions have been selected by redundancy analysis. Two structural equation models were established to compare the roles of OPDB and IPSB on P availability during composting. Variance partitioning further showed that the interactions between IPSB and OPDB communities had a greater impact on P transformation than each independent factor. Therefore, the combined regulation of IPSB and OPDB were suggested to control the transformation of P fractions during composting.