ABSTRACT
OBJECTIVE: To determine the content of protocatechuic acid in Blumea riparia by RP-HPLC. METHOD: mu-Bondapak C18 column was used, mobile phase consisted of methanol-water-glacial acetic acid(19:80:1) and detection was performed at UV 260 nm. RESULT: The standard curve was linear in the range of 3.31-41.8 micrograms.ml-1. The correlation coefficient was 0.9999. The average recovery rate and RSD were 98.05% and 1.94% (n = 6) respectively. CONCLUSION: The method provides scientific indexes for quality control of riparia.
Subject(s)
Asteraceae/chemistry , Hydroxybenzoates/analysis , Plants, Medicinal/chemistry , Chromatography, High Pressure Liquid/methodsABSTRACT
Identification and determination of borneol and isoborneol in ointment Liyan Gao by TLC and GC were carried out. L-borneol standard substance and external standard one-point method were used for the determination. The recommended method is precise and simple.