ABSTRACT
To evaluate the effects of different combinations of probiotics on performance, egg quality, and immune response of layer hens, a trial was carried out with 1,800 white feather layer hens of the Lohmann variety. The experiment was conducted by using a completely randomized design with 9 treatments, 4 replicates, and 50 hens in each replicate. Compared with the control group, group F, which added a composition of heat-inactivated Lactobacillus salivarius(CB) and Bacillus subtilis to the diets of layer hens, caused highly significant (P < 0.05) increases in egg production, daily egg yield, damaged egg ratio, combined with a significant (P < 0.05) decrease in feed conversion and damaged egg ratio. Group G, adding a combination of inactivated Lactobacillus salivarius and sodium butyrate, resulted in a significant increase (P < 0.05) in daily egg yield, feed conversion, damaged egg ratio and Haugh unit. Meanwhile, groups D and H had significantly decreased feed conversion (P < 0.05), and groups B, H, and I had a significantly decreased damaged egg ratio. In serum levels, no significant difference was observed except a significant decrease (P < 0.05) in total cholesterol (groups D, E, and G) and low-density lipoprotein cholesterol (group E and G) and a significant increase (P < 0.05) in total cholesterol (groups D, E, and G) compared with group A. According to the hemagglutination inhibition test, the antibody titer of antibody against the avian influenza virus was significantly (P < 0.05) higher in most treated groups such as groups B, C, E, G, and I after d 15 fed to layers with probiotics and groups B, C, D, E, F, G, and H after d 45 compared with the control group. No significant difference was observed in the antibody titer against the Newcastle disease virus at d 15, but significantly (P < 0.05) higher at d 45 in groups F and G. These results demonstrate that several combinations of probiotics used in this experiment have a positive impact on the performance, egg quality, and immune response of layer hens, and the following work will continue to focus on these groups.
Subject(s)
Butyrates/pharmacology , Chickens/physiology , Eggs/standards , Oviposition/drug effects , Probiotics/pharmacology , Animal Feed/analysis , Animal Nutritional Physiological Phenomena , Animals , Antibodies/blood , Chickens/blood , Cholesterol/blood , Diet/veterinary , Dietary Supplements , Female , Hemagglutination TestsABSTRACT
OBJECTIVE: To study Cordyceps (artificial fermented Cordyceps sinensis(Berk.) Sacc) powderin the treatment of asthma in the animal models. METHOD: Pulmonary function and airway inflammation in vivo were investigated. RESULT: Cordyceps, 5g.kg-1(ig), significantly inhibited bronchial challenge of ovalbumin-induced change of RL and Cdyn (P < 0.05) and inhibited antigen-induced increase of eosinophils in the BALF of rats (P < 0.05). CONCLUSION: The results suggested cordyceps could be applied for the prevention and cure of asthma.
Subject(s)
Asthma/physiopathology , Cordyceps , Drugs, Chinese Herbal/pharmacology , Lepidoptera , Lung/physiopathology , Phytotherapy , Airway Resistance/drug effects , Animals , Asthma/drug therapy , Cordyceps/chemistry , Drugs, Chinese Herbal/isolation & purification , Female , Guinea Pigs , Lepidoptera/chemistry , Lung Compliance/drug effects , Male , Rats , Rats, Sprague-DawleyABSTRACT
From the ethyl acetate extract of Asarum forbesii Maxin, four new constituents, asarumin A(I), B(II), C(III) and D(IV), were isolated along with elemicin (V), trans-asarone(VI) and linoleic acid(VII). The structures of the new compounds were elucidated as methyl 3 S-benzoyloxy-2 S-hydroxy-2-isopropylbutyrate for I, methyl 2 R-benzoyloxyisopentanoate for II, methyl 2 R-trans-cinnamoyloxyisopentanoate for III and methyl 2 R-piperonyloyloxyisopentanoate for IV. Compounds I, II, III and VII showed weak inhibition of PCA in rats, but the other compounds were inactive.