Sequential targeting biomimetic nano platform for enhanced mild photothermal therapy and chemotherapy of tumor.

Tumor targeting drug delivery is of significant importance for the treatment of triple negative breast cancer (TNBC) considering the presence of appreciable amount of tumor matrix and the absence of effective targets on the tumor cells. Hence in this study, a new therapeutic multifunctional nanoplatform with improved TNBC targeting ability and efficacy was constructed and used for therapy of TNBC. Specifically, curcumin loaded mesoporous polydopamine (mPDA/Cur) nanoparticles were synthesized. Thereafter, manganese dioxide (MnO2) and a hybrid of cancer-associated fibroblasts (CAFs) membranes as well as cancer cell membranes were sequentially coated on the surface of mPDA/Cur to obtain mPDA/Cur@M/CM. It was found that two distinct kinds of cell membranes were able to endow the nano platform with homologous targeting ability, thereby achieving accurate delivery of drugs. Nanoparticles gathered in the tumor matrix can loosen the tumor matrix via the photothermal effect mediated by mPDA to rupture the physical barrier of tumor, which is conducive to the penetration and targeting of drugs to tumor cells in the deep tissues. Moreover, the existence of curcumin, MnO2 and mPDA was able to promote the apoptosis of cancer cells by promoting increased cytotoxicity, enhanced Fenton-like reaction, and thermal damage, respectively. Overall, both in vitro and in vivo results showed that the designed biomimetic nanoplatform could significantly inhibit the tumor growth and thus provide an efficient novel therapeutic strategy for TNBC.

Preparation, optimization and reusability of immobilized petroleum-degrading bacteria.

In the remediation of marine pollution, it is important to effectively degrade pollutants and reuse petroleum-degrading bacteria. In order to obtain more effective biodegradability and reusability, an immobilized bacteria combination with petroleum-degrading bacteria, sodium alginate (SA) and biochar by adsorption-embedding method was systematically analysed. The results indicated that the immobilized bacteria had good mechanical properties and the degradation rate was 51% when the straw (CS) was 3%, the SA and CaCl2 were 4.5% and 6%, respectively. Besides, SA-CS-DM-PVA has the highest degradation rate and the lowest broken rate, above 51% and below 6.1% respectively. The optimum dosage of the modified immobilized bacteria was 132, degradation time was 5d, and reuse frequency was 4 times. Moreover, immobilized bacteria characterized by scanning electron microscopy (SEM), results showed that there were more pores on the surface after degradation, and the carrier was exposed. Therefore, the modified immobilized bacteria with good degradability and reusability, have good application prospects in the treatment of marine oil pollution.