ABSTRACT
OBJECTIVE: To explore the effect of Ligustrazine (LGZ) affecting the expression of CD40 on mesothelial cells (MC). METHODS: Rat's peritoneal MC isolated from peritoneal cavity were cultured and maintained under the defined conditions in vitro. They were cultured with 4.25% peritoneal dialysis solution (PDS), 4.25% PDS added LGZ (40 mg/L), 4.25% PDS added IFN-gamma (100 u/ml) and 4.25% PDS added IFN-gamma and LGZ for 30 min, the DMEM/F12 cultural medium was taken as control. The expression of CD40 on MC was detected by reverse transcript polymerase chain reaction (RT-PCR) and flow cytometry analysis. RESULTS: MC cultured in vitro expressed CD40 markedly, the expression of CD40 mRNA and its protein was markedly up-regulated following stimulation with 4.25% PDS or IFN-gamma and 4.25% PDS. LGZ (40 mg/L) could reduce the expression of CD40 mRNA and its protein significantly as it was added in 4.25% PDS and 4.25% PDS with IFN-gamma. CONCLUSION: The PDS stimulates the expression of CD40 on MC. LGZ has the function of down-regulating the expression of CD40 on MC. During long-term continuous ambulatory peritoneal dialysis, it might be helpful to alleviate chronic inflammatory reaction by adding LGZ into standard PDS, therefore prevent or retard the occurence and development of peritoneal fibrosis.
Subject(s)
CD40 Antigens/biosynthesis , Epithelium/metabolism , Peritoneum/cytology , Pyrazines/pharmacology , Animals , Anti-Inflammatory Agents, Non-Steroidal/pharmacology , CD40 Antigens/genetics , Cells, Cultured , Dialysis Solutions/pharmacology , Fibrosis/prevention & control , Male , Peritoneal Dialysis/adverse effects , RNA, Messenger/biosynthesis , RNA, Messenger/genetics , RatsSubject(s)
Apoptosis/drug effects , Drugs, Chinese Herbal/pharmacology , Kidney/pathology , Lupus Nephritis/pathology , Proto-Oncogene Proteins c-myc/biosynthesis , Cell Division/drug effects , Cells, Cultured , Fibroblasts/metabolism , Fibroblasts/pathology , Humans , Kidney/metabolism , Lupus Nephritis/metabolism , Plant Extracts , Salvia miltiorrhizaABSTRACT
The therapy of integrated traditional Chinese medicine and Western medicine (TCM-WM) on repeatedly relapsed primary nephrotic syndrome (PNS) was observed. There were 30 and 32 cases in the WM group and TCM-WM group respectively. The results showed that in WM group the recurrence rate was 11.8%, 23.5%, 35.3% at 6,12,47 months after the PNS was remitted, while in TCM-WM group, it was 0, 3.3%, 13.3% respectively. The mean remission period was 12.4 +/- 3.2 months and 35.9 +/- 3.7 months in WM group and TCM-WM group respectively. The recurrence rate in TCM-WM group at various time was significantly lower than that in WM group (P < 0.01). The mean remission period in TCM-WM group was also significantly longer than that in WM group (P < 0.01). This indicated that the therapy of TCM-WM on repeatedly relapsed PNS is superior than that of WM alone.
Subject(s)
Drugs, Chinese Herbal/therapeutic use , Nephrotic Syndrome/drug therapy , Prednisone/therapeutic use , Adolescent , Adult , Cyclophosphamide/therapeutic use , Female , Humans , Male , Middle Aged , RecurrenceABSTRACT
The therapy of integrated traditional Chinese medicine and Western medicine (TCM-WM) on lupus nephritis (LN) was explored. There were 74 and 66 cases in the TCM-WM group and WM group respectively. The results showed that in TCM-WM group the effective rate and recurrence rate were 95.9% and 4.1% respectively, while in WM group they were 80.3% and 21.2% respectively. The occurrence rate of side effect was 37.9% and 77.3% in TCM-WM group and WM group respectively. It indicated that the therapy of TCM-WM could significantly enhance curative rate and reduce recurrence and side effect rate.
Subject(s)
Drugs, Chinese Herbal/administration & dosage , Lupus Nephritis/drug therapy , Prednisone/administration & dosage , Adolescent , Adult , Child , Cyclophosphamide/administration & dosage , Drug Therapy, Combination , Female , Humans , MaleABSTRACT
UNLABELLED: 41 active lupus nephritis patients were treated vigorously with cyclophosphamide (CTX), steroid used as routine, as well as TCM administered according to the various disease stage. 35 cases administered with same western medicine but no TCM was taken as control group. RESULT: After a six-month treatment course, the therapeutical efficacy was significantly higher in treated group than that in control group (P < 0.05). Lupus nephritis usually manifested itself as Liver-Kidney Yin deficiency in TCM. Our study suggested that Chinese herbs might play an important role in the treatment of lupus nephritis.
Subject(s)
Cyclophosphamide/administration & dosage , Drugs, Chinese Herbal/therapeutic use , Lupus Nephritis/drug therapy , Adolescent , Adult , Child , Female , Humans , Male , Middle Aged , Yin Deficiency/drug therapyABSTRACT
UNLABELLED: The model of experimental glomerulonephritis in situ in rats was established by administration of C-BSA. 24 male Wistar rats were randomly assigned to three groups: C-BSA, control and C-BSA+scorpion (scorpion injection, ip, 1.0 g/kg, once/day). At the end of 4th week, the bioactivity of serum IL-1 was assayed by thymocyte proliferation method and the renal pathology was checked up. Urine sample was collected for 24 hour once/week for protein determination. RESULT: Quantity of proteinuria in scorpion group was reduced significantly in comparing with C-BSA group. Level of IL-1 was significantly lower in scorpion group than that in C-BSA group. Injury of renal tissue also was mild in the treated group. These results suggested that scorpion injection could suppress the activities of IL-1 and ameliorate the pathological injury in this experimental glomerulonephritis in situ.
Subject(s)
Glomerulonephritis/drug therapy , Interleukin-1/blood , Materia Medica/therapeutic use , Scorpions , Animals , Glomerulonephritis/immunology , Glomerulonephritis/pathology , Kidney Cortex/pathology , Male , Mice , Mice, Inbred C3H , Random Allocation , Rats , Rats, Wistar , Serum Albumin, BovineABSTRACT
This paper studied the method and effect of adult primary nephrotic syndrome (PNS) with the integrated traditional Chinese and Western medicine (TCM-WM) treatment. 134 patients were divided into WM group (66 cases) and TCM-WM group (68 cases). Both groups were mainly treated with corticosteroid. The latter group was treated with corticosteroid plus Chinese medicinal herbs. The results showed that total effective rate of WM group and TCM-WM group was 56.1% and 85.3% respectively, while the occurrence rate of side effect was 48% and 14.8% respectively. It indicated that corticosteroid plus Chinese medicinal herbs could enhance curative effect and reduce side effect.
Subject(s)
Drugs, Chinese Herbal/therapeutic use , Nephrotic Syndrome/drug therapy , Prednisone/therapeutic use , Adolescent , Adult , Cushing Syndrome/chemically induced , Cushing Syndrome/drug therapy , Drug Therapy, Combination , Female , Humans , Male , Middle Aged , Prednisone/adverse effects , Substance Withdrawal Syndrome/drug therapy , Yang Deficiency/chemically induced , Yang Deficiency/drug therapy , Yin Deficiency/chemically induced , Yin Deficiency/drug therapyABSTRACT
45 cases of IgA nephropathy were confirmed by renal biopsy and were divided into 2 groups, the integrated traditional and western medicine (TCM-WM) therapeutic group and the western medicine (WM) therapeutic group. The WM group was treated by conventional western medicine (persantin, vitamin B co.), and the TCM-WM group was treated by the same western medicine plus differential symptomatic treatment with decoction of Chinese crude herbs. In this paper, most patients (30 cases) were present as deficiency of Yin of the Liver and Kidney type, and were treated by Qiju Dihuang pill plus Erzhi pill. All types in TCM-WM group were also used herbs of promoting blood circulation and remove blood stasis in almost every case. After 6 months of such treatment, the total effective rate of TCM-WM group and the WM group were 86.4% and 13.0% respectively. There was very significant difference (P less than 0.01) between them. The results showed that the curative effect of the TCM-WM group was much superior than WM alone for the treatment of IgA nephropathy.