ShCIGT, a Trihelix family gene, mediates cold and drought tolerance by interacting with SnRK1 in tomato.

Abiotic stress, such as drought and cold stress, have a major impact on plant growth and development. The trihelix transcription factor family plays important roles in plant morphological development and adaptation to abiotic stresses. In this study, we isolated a cold-induced gene named ShCIGT from the wild tomato species Solanum habrochaites and found that it contributes to abiotic stress tolerance. ShCIGT belongs to the GT-1 subfamily of the trihelix transcription factors. It was constitutively expressed in various tissues. Its expression was induced by multiple abiotic stresses and abscisic acid (ABA). Overexpression of ShCIGT in cultivated tomato enhanced cold and drought stress tolerance. In addition, the transgenic plants displayed a reduced sensitivity to ABA during post-germination growth. We found that ShCIGT interacts with SnRK1, an energy sensor in the metabolic signaling network, which controls plant metabolism, growth and development, and stress tolerance. Based on these data, we conclude ShCIGT may improve abiotic-stress tolerance in tomato by interacting with SnRK1.

Overexpression of ShDHN, a dehydrin gene from Solanum habrochaites enhances tolerance to multiple abiotic stresses in tomato.

Dehydrins (DHNs) play important roles in plant adaptation to abiotic stress. In this study, a cold-induced SK3-type DHN gene (ShDHN) isolated from wild tomato species Solanum habrochaites was characterized for its function in abiotic stress tolerance. ShDHN was constitutively expressed in root, leaf, stem, flower and fruit. ShDHN was continuously up-regulated during cold stress and showed higher expression level in the cold-tolerant S. habrochaites than in the susceptible S. lycopersicum. Moreover, ShDHN expression was also regulated by drought, salt, osmotic stress, and exogenous signaling molecules. Overexpression of ShDHN in cultivated tomato increased tolerance to cold and drought stresses and improved seedling growth under salt and osmotic stresses. Compared with the wild-type, the transgenic plants accumulated more proline, maintained higher enzymatic activities of superoxide dismutase and catalase, and suffered less membrane damage under cold and drought stresses. Moreover, the transgenic plants accumulated lower levels of H2O2 and O2(-) under cold stress, and had higher relative water contents and lower water loss rates under dehydration conditions. Furthermore, overexpression of ShDHN in tomato led to the up- or down-regulated expression of several genes involved in ROS scavenging and JA signaling pathway, including SOD1, GST, POD, LOX, PR1 and PR2. Taken together, these results indicate that ShDHN has pleiotropic effects on improving plant adaptation to abiotic stresses and that it possesses potential usefulness in genetic improvement of stress tolerance in tomato.

Differential modulation of photosynthesis, signaling, and transcriptional regulation between tolerant and sensitive tomato genotypes under cold stress.

The wild species Solanum habrochaites is more cold tolerant than the cultivated tomato (S. lycopersicum). To explore the mechanisms underlying cold tolerance of S. habrochaites, seedlings of S. habrochaites LA1777 introgression lines (ILs), as well as the two parents, were evaluated under low temperature (4°C). The IL LA3969 and its donor parent LA1777 were found to be more cold tolerant than the recurrent parent S. lycopersicum LA4024. The differences in physiology and global gene expression between cold-tolerant (LA1777 and LA3969) and -sensitive (LA4024) genotypes under cold stress were further investigated. Comparative transcriptome analysis identified 1613, 1456, and 1523 cold-responsive genes in LA1777, LA3969, and LA4024, respectively. Gene ontology (GO) term enrichment analysis revealed that more GO biological process terms were significantly enriched among the up-regulated genes in the two tolerant genotypes, whereas more biological processes were significantly repressed by cold stress in the sensitive one. A total of 92 genes with significant differential expression between tolerant and sensitive genotypes under cold stress were identified. Among these, many stress-related GO terms were significantly enriched, such as 'response to stimulus' and 'response to stress'. Moreover, GO terms 'response to hormone stimulus', 'response to reactive oxygen species (ROS)', and 'calcium-mediated signaling' were also overrepresented. Several transcripts involved in hormone or ROS homeostasis were also differentially expressed. ROS, hormones, and calcium as signaling molecules may play important roles in regulating gene expression in response to cold stress. Moreover, the expression of various transcription factors, post-translational proteins, metabolic enzymes, and photosynthesis-related genes was also specifically modulated. These specific modifications may play pivotal roles in conferring cold tolerance in tomato. These results not only provide new insights into the molecular mechanisms of cold tolerance in tomato, but also provide potential candidate genes for genetic improvement.