Study on HPLC-fingerprint-based identification of dao-di herb and non dao-di herb of scutellariae radix / 中国中药杂志

<p><b>OBJECTIVE</b>To compare the discrepancies between chemical constituents in Dao-di herb and non Dao-di herb of Huangqin (the root of Scutellaria baicalensis), study the impact of habitat and growth pattern (including cultivated and wild Huangqin) on chemical substances of Huangqin, and then provide evidence for the identification of Dao-di herb and quality evaluation of Huangqin.</p><p><b>METHOD</b>The chemical constituents in Huangqin collected from different habitats and under different growth patterns, were analyzed using HPLC fingerprint. The fingerprints obtained were then evaluated by hierarchical clustering analysis, principal component analysis and components peak area pattern.</p><p><b>RESULT</b>The fingerprints' chemical profiles of Dao-di herb and non Dao-di Huangqin had significant disparity. The fingerprints of modem Dao-di herb Huangqin samples originated from Chengde (Hebei Province) were significantly different from those from other habitats, though the fingerprints of the non Dao-di Huangqin collected from Chifeng (Inner Mongolia) and Chengde had high similarity to each other. The chemical characteristics of Huangqin samples collected from the habitats recorded in ancient herbals, such as Qingyang (Gansu Province), Yan'an (Shaanxi Province), Linyi (Shangdong Province), Changzhi and Jinzhong (Shanxi Province) were similar. The fingerprints of modern non Dao-di samples collected from Dingxi and Longnan (Gansu Province) and Shangluo (Shaanxi Province) had high similarity. In addition, the content of acteoside in wild Huangqin was higher than that in cultivated Huangqin.</p><p><b>CONCLUSION</b>Dao-di herb and non Dao-di herb of Huangqin could be distinguished using the developed HPLC fingerprints. The results obtained may provide evidence for the quality control and pharmcodynamical research of Dao-di herb and non Dao-di Huangqin.</p>

Phenolic acid derivatives from Bauhinia glauca subsp. pernervosa / 药学学报

To study the chemical constituents of Bauhinia glauca subsp. pernervosa, eleven phenolic acids were isolated from a 95% ethanol extract by using a combination of various chromatographic techniques including column chromatography over silica gel, ODS, MCI, Sephadex LH-20, and semi-preparative HPLC. By spectroscopic techniques including 1H NMR, 13C NMR, 2D NMR, and HR-ESI-MS, these compounds were identified as isopropyl O-beta-(6'-O-galloyl)-glucopyranoside (1), ethyl O-beta-(6'-O-galloyl)-glucopyranoside (2), 3, 4, 5-trimethoxyphenyl-(6'-O-galloyl)-O-beta-D-glucopyranoside (3), 3, 4, 5-trimethoxyphenyl-beta-D-glucopyranoside (4), gallic acid (5), methyl gallate (6), ethyl gallate (7), protocatechuic acid (8), 3, 5-dimethoxy-4-hydroxybenzoic acid (9), erigeside C (10) and glucosyringic acid (11). Among them, compound 1 is a new polyhydroxyl compound; compounds 2, 10, and 11 were isolated from the genus Bauhinia for the first time, and the other compounds were isolated from the plant for the first time. Compounds 6 and 8 showed significant protein tyrosine phosphatase1B (PTP1B) inhibitory activity in vitro with the IC50 values of 72.3 and 54.1 micromol x L(-1), respectively.

Isoflavones from Millettia nitida var. hirsutissima / 药学学报

To study the chemical constituents of Millettia nitida var. hirsutissima, the constituents were isolated by chromatographic techniques, and structures were identified by spectroscopic methods. Eight isoflavones were isolated and identified, including a new compound, hirsutissimiside F (1), and seven known compounds, formononetin (2), ononin (3), odoratin 7-O-beta-D-glucopyranoside (4), lanceolarin (5), afromosin (6), sphaerobioside (7), and hirsutissimiside B (8). Compounds 3, 4, 5 and 7 were isolated from the genus Millettia for the first time, 2 was obtained from this plant for the first time.

Chemical constituents of Momordica charantia L / 药学学报

Momordica charantia L. is a vegetable widely cultivated around the world. Its fruits have been used in Asian countries as a folk medicine for the treatment of non-insulin-dependent diabetes mellitus. Here we report eight compounds isolated from the fruits of M. charantia. On the basis of NMR and MS spectroscopic analyses, these compounds were identified as momordicolide ((10E)-3-hydroxyl-dodeca-10-en-9-olide, 1), monordicophenoide A (4-hydroxyl-benzoic acid 4-O-beta-D-apiofuranosyl (1 --> 2)-O-beta-D-glucopyranoside, 2), dihydrophaseic acid 3-O-beta-D-glucopyranoside (3), 6,9-dihydroxy-megastigman-4,7-dien-3-one (blumenol, 4), guanosine (5), adenosine (6), uracil (7) and cytosine (8). Among them, 1 and 2 are new compounds. Compounds 3-5 were isolated from this plant for the first time.

Studies on chemical constituents from seed of Psoralea corylifolia / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of Psoralea corylifolia.</p><p><b>METHOD</b>Chromatographic methods were used to separate compounds, and spectroscopic methods were used to determine the structures.</p><p><b>RESULT</b>Five compounds were isolated and identified as furano (2", 3", 7, 6)-4'-hydroxyflavanone (1), psoralidin (2), genistein (3), psoralen (4), isopsoralen (5).</p><p><b>CONCLUSION</b>Compounds 1 was a new compound named psoraleflavanone. And 13C-NMR data of compound 2 were assined for the first time.</p>

Effect of supplemented Taoren Chengqi decoction on NIT-1, a pancreatic beta-cell from a transgenic NOD/Lt mouse / 中国中药杂志

<p><b>OBJECTIVE</b>To study the effect of Supplemented Taoren Chengqi decoction (STCD) on the secretion of insulin and proliferation of NIT-1.</p><p><b>METHOD</b>The effect of STCD and the serum of rat after orally administrating of STCD on the secretion of insulin and proliferation of NIT-1 were studied. The proliferation of NIT-1 was measured by 3H-TdR incorporation and cell counting methods, while the secretion of insulin was measured from the cultured medium by the ultra sensitive rat insulin ELISIA kit.</p><p><b>RESULT</b>Both the STCD and the serum of rat after orally administrating of STCD significantly could increased the secretion of insulin and proliferation of NIT-1.</p><p><b>CONCLUSION</b>The treatment of the diabetic patients by STCD might be through with its improvement of secretion of insulin and proliferation on pancreatic beta-cell.</p>

Chemical constituents from Spatholobus sinensis / 药学学报

Spatholobus sinensis is a plant of the Spatholobus genus (Leguminosae family). Its caulis are used as "ji-xue-teng" regionally. However, to our knowledge, no phytochemical investigation on S. sinensis has been reported to date. In this study, eight compounds were isolated from the ethanol extract of the caulis of S. sinensis, by solvents extraction and column chromatography methods. By analysis of their physic-chemical constants and spectral data, the structures of 8 compounds were identified as spatholosineside A (1), 2',4',5,7-tetrahydroxyisoflavone (2), isoliquiritigenin (3), lupinalbin A (4), coumestrol (5), naringenin (6), protocatechuic acid (7), leonuriside A (8). Compound 1 is a new compound.

Study on biligustilides from Angelica sinensis / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of Angelica sinensis.</p><p><b>METHOD</b>The constituents were separated by chromatographic methods, and their structures were identified on the basis of spectroscopic analysis.</p><p><b>RESULT</b>Eight compounds were isolated and identified as levistolide A (1), senkyunolide O (2), (3Z, 3Z')-6.8', 7.3'-diligustilide (3), tokinolide B (4), isotokinolide B (5), (3'Z)-(3R, 8S, 3a'R, 6'S)-3, 3a': 8, 6'-biligustilide (6), E, E'-3. 3', 8. 8'-diligustilide (7) and E, E'-3. 3', 8. 8'-isodiligustilide (8), which are all diligustilides.</p><p><b>CONCLUSION</b>Compound 7 was obtained from the plant for the first time; compounds 6 and 8 are new compounds.</p>

Main flavonoids from Sophora flavescenes / 药学学报

Radix Sophorae Flavescentis is a traditional Chinese medicine, which is the roots of Sophora flavescens and is used to treat abscess, dysentery, eczema and skin itch. The main active compounds of the roots of Sophora flavescens are alkaloids and flavonoids. To provide reference compounds for HPLC-DAD qualitification analysis of flavanonoids and for study on the bioactivities of flavanonoids, six main flavanonoids were isolated from the roots of Sophora flavescens. They are 5-methoxy-7, 2', 4'-trihydroxy-8-[3,3-dimethylallyl] -flavanone (1), isoxanthohumol (2), 3/beta, 7, 4'-trihydroxy-5-methoxy-8-[3,3-dimethylallyl] -flavanone (3), nor-kurarinone [(2S) -sophoraflavanone G] (4), kurarinone (5) and kushenol I (6). Compound 1 is a new compound, and compound 3 is obtained from Sophora flavecens for the first time. The 13C NMR data of compounds 3 and 5 are reported for the first time.

Studies on chemical constituents of Tibetan medicine Caryopteris toroetii / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of the Tibetan medicine Caryopteris toroetii.</p><p><b>METHOD</b>The crude drug was extracted with 95% EtOH and isolated by repeatedly chromatographic methods. The structures of the isolated compounds were elucidated by spectral analysis.</p><p><b>RESULT</b>Six compounds were yielded and identified as: phenethyl cinnamide (1), 1-hydroxy-2, 3, 4, 5-tetramethoxy-xanthone (2), 1-hydroxy-2, 3, 5-trimethoxy-xanthone (3), (2S)-5-hydroxy-7-methoxyflavanone (4), 2', 4'-dihydroxydihydrochalcone (5) and rutin (6).</p><p><b>CONCLUSION</b>All the above compounds were obtained from C. toroetii for the first time.</p>

Chemical constituents of isoflavones from vine stems of Millettia nitita var. hirsutissima / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents from the vine stems of isoflavones of Millettia nitita var. hirsutissima.</p><p><b>METHOD</b>Chromatographic methods were used to isolate compounds, and spectroscopic methods were used to elucidate the structures.</p><p><b>RESULT</b>Four isoflavones were isolated and identified: 3'-O-methylorobol (1), genistein (2), biochanin A (3), afromosin (4).</p><p><b>CONCLUSION</b>All Compounds were obtained from the plant for the first time.</p>

Saponins from roots of Hedysarum multijugum / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents from the root of Hedysarum multijugum.</p><p><b>METHOD</b>The compounds were separated by chromatographic methods, their structures were identified by spectral analysis.</p><p><b>RESULT</b>Four compounds were isolated and identified as soyasaponin II methyl ester (1), soyasaponin II (2), soyasaponin Bg (3), and soyasaponin I (4).</p><p><b>CONCLUSION</b>The four compounds were obtained from the plant for the first time. Compound 3 was isolated from the genus Hedysarum for the first time, and the NMR data of 3 was reported for the first time.</p>

Study on chemical constituents of mangrove Acanthus ilcifolius / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of Acanthus ilicifolius.</p><p><b>METHOD</b>Chromatographic methods were used to isolate compounds from A. ilicifolius, and chemical and spectroscopic methods were used to elucidate the structures of the isolated compounds.</p><p><b>RESULT</b>Seven compounds, betaine (1), phenylethyl-O-beta-D-glucopyranosyl- (1-->2) -beta-D-glucopyranoside (2), phenylethyl-O-beta-D-glucopyranoside (3), acteoside (4), isoacteoside (5), benzyl-O-beta-D-glucopyranoside (6) and vanillic acid (7) were obtained.</p><p><b>CONCLUSION</b>1, 3, 6 and 7 were obtained from the genus for the first time.</p>

Studies on chemical constituents in flower of Abelmoschus manihot / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of Abelmoschus manihot.</p><p><b>METHOD</b>Chromatographic methods were used to isolate compounds from A. manihot, and spectroscopic methods were used to identify the structures.</p><p><b>RESULT</b>Thirteen compounds, myricetin (1), cannabiscitrin (2), myricetin-3-O-beta-D-glucopyranoside (3), glycerolmonopalmitate (4), 2, 4-dihydroxy benzoic acid (5), guanosine (6), adenosine (7), maleic acid (8), heptatriacontanoic acid (9), 1-triacontanol (10) , tetracosane (11), beta-sitosterol (12), beta-sitosterol-3-O-beta-D-glucoside (13) were obtained.</p><p><b>CONCLUSION</b>2-11 were obtained from the genus for the first time.</p>

Studies on lignan glycosides from the roots of Bupleurum scorzonerifolium / 药学学报

<p><b>AIM</b>To study the lignan glycosides from the roots of Bupleurum scorzonerifolium. Chromatography methods was used to isolate compounds and chemical and spectral methods were used to identify the structures of isolated compounds.</p><p><b>RESULTS</b>Two lignan glycosides were isolated from the roots of B. scorzonerifolium and their structures were identified as 2, 3-E-2,3-dihydro-2-(3'-methoxy-4'-O-beta-D-glucopyranosyl-phenyl)-3-hydroxymethyl-5-(3"-hydroxypropenyl )-7-methoxy-1-benzo [b] furan (1) and 2, 3-E-2, 3-dihydro-2-( 3'-methoxy-4'-hydroxy-phenyl)-3-hydroxymethyl-5-( 3"-hydroxypropenyl)-7-O-beta-D-glucopyranosyl-1-benzo[b]furan (2), both of them which were the mixture of (+)2S,3R- and (-)2R,3S-type diastereoisomer and in both of them the amount of (+)2S,3R-type was a little more than that of the (-)2R,3S-type.</p><p><b>CONCLUSION</b>Compounds 1 and 2 were isolated from B. scorzonerifolium for the first time. (+)2S, 3R-2, 3-Dihydro-2-(3'-methoxy-4'-hydroxy-phenyl)-3-hydroxymethyl-5-(3"-hydroxypropenyl)-7-O-beta-D-glucopyranosyl-1-benzo[b] furan is a new compound.</p>

Chemical constituents of the rhizome of Matteuccia struthiopteris / 药学学报

<p><b>AIM</b>To study the chemical constituents of the rhizome of Matteuccia struthiopteris (L.) The constituents were separated and purified by column chromatography with normal Todaro.</p><p><b>METHODS</b>phase silica gel and Sephadex LH-20. Their structures were identified on the basis of physical and spectral data (MS, NMR, HMBC and HMQC).</p><p><b>RESULTS</b>Four compounds were isolated and identified as 1-O-beta-D-gl ucopyranosyl-(2S,3R,4E, 8Z) -2-N-(2'-hydroxydocosanoyl) eicosasphinga-4,8-dienine (1), 1-O-beta-D-galactosyl-(6-->1)-alpha-D-galactosyl-2,3-O-dihexadecanoyl-glycerol (2), succinic acid (3), D-mannitol (4).</p><p><b>CONCLUSION</b>Compounds 1 and 2 are new compounds. Compounds 3 and 4 were isolated from this plant for the first time.</p>

The megastigman glycosides from herb of Potentilla multifida / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the chemical constituents of Potentilla multifida.</p><p><b>METHOD</b>Various chromatographic techniques were employed for isolation and purification of the constituents. The structures were elucidated by spectral analysis.</p><p><b>RESULT</b>Four megastigmane glycosides were isolated from P. multifida and their structures were identified as citroside A (1), icariside B1 (2), (6S,7E,9R)-roseoside (3), (6S,7E,9R)-vomifoliol-9-O-beta-D-xylopyranosyl-(1-->6)-O-beta-D-glucopyranoside (4), respectively.</p><p><b>CONCLUSION</b>All compounds were obtained from the genus Potentilla for the first time.</p>

Studies on chemical constituents in herbs of Acanthus ilicifolius / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of Acanthus ilicifolius.</p><p><b>METHOD</b>Chromatographic methods were used to isolate compounds from A. ilicifolius, and chemical and spectral methods were used to elucidate the structures of the isolated compounds.</p><p><b>RESULT</b>Five compounds, luteolin 7-O-beta-D-glucuronide (1), apigenin-7-O-beta-D-glucuronide (2), methylapigenin-7-O-beta-D-glucuronate (3), uridine (4) and uracil (5) were obtained.</p><p><b>CONCLUSION</b>1, 4 and 5 were obtained from the genus for the first time.</p>

Studies on chemical constituents from root of Hedysarum polybotrys / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of Hedysarum polybotrys.</p><p><b>METHOD</b>Chromatographic methods were used to isolate compounds from H. polybotrys and chemical and spectral methods were used to elucidate the structures of the isolated compounds.</p><p><b>RESULT</b>Five compounds, N,N,N-trimethyl-tropaphone inner salt (hypaphorine) (1), octadecyl-3-methoxy-4-hydroxy-benzeneacrylate (2), 5,7,4'-trihydroxy-dihydroflavanone 5,7-di-O-beta-D-glucopyranoside (3), 3,4,5-trimethoxy cinnamic acid methy ester (4) and vanillic acid (5), were isolated from the roots of H. polybotrys.</p><p><b>CONCLUSION</b>Compounds 1, 2 and 3 were obtained from this plant for the first time, while compounds 1 and 2 were isolated from Hedysarum for the first time.</p>

Studies on chemical constituents in rhizome of Matteuccia struthiopteris / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents in rhizome of Matteuccia struthiopteris.</p><p><b>METHOD</b>The compounds were isolated by normal phase silica gel chromatography. The structures were identified by physical and spectral data.</p><p><b>RESULT</b>Six compounds were isolated and identified as woodwardic acid (1), ergost-6,22-diene-3beta,5alpha,8alpha-triol (2), apigenin (3), riboflavin (4), 4-O-beta-D-glucopyranosyl-p-coumaric acid (5), 4-O-beta-D-glucopyranosylcaffeic acid (6).</p><p><b>CONCLUSION</b>All the compounds were obtained from this plant for the first time.</p>