[Special culture mediums of breeding clones of Dendrobium officinale].

The explants were obtained from stem segments of hybrid combination of Dendrobium officinale germplasms (Zhejiang Yandang Mountain x Yunnan Guangnan). The screened culture mediums for buds induction and multiplication, which were 1/2MS + IBA 1.0 mg x L(-1) +6-BA 1.0 mg x L(-1) and 1/2MS + IBA 1.5 mg x L(-1) +6-BA 0.5 mg x L(-1) respectively, were applied to 159 germplasms of D. officinale from Zhejiang, Yunnan, Guangxi, Hunan, etc. The medium for axillary buds induction had universality with 94.3% induction rate. During buds multiplication, there were significant differences in proliferation effect among germplasms, and three proliferation forms i.e. single bud, multiple buds and protocorm were differentiated from different germplasms. The results showed that different germplasms of D. officinale had specific requirements for culture medium. Therefore, developing special culture medium for breeding clones of D. officinale is urgent and has important application values.

[Antitumour effect of total saponins of Rubus parvifolius on malignant melanoma].

OBJECTIVE: To evaluate the antitumor effect of total saponins of R. parvifolius on malignant melanoma. METHOD: The human malignant melanoma A375 cells were regularlly subcultured in vitro, and were divided into five groups contained positive control group (CTX), high concentration (0.01 mg x mL(-1)) and middle concentration (0.001 mg x mL(-1)) and low concentration (0.000 1 mg x mL(-1)) total saponins of R. parvifolius groups and negative control group. By using MTT colorimetric method, the cell viability was measured. B16 melanoma cells were transplanted to mice, which were divided into positive control group, high dose (100 mg x kg(-1)) and middle dose (50 mg x kg(-1)) and low dose (25 mg x kg(-1)) total saponins of R. parvifolius groups and negative control group. The inhibition effect of the tumor in vivo, mean survival time and rate of life-elongation of the mice were observed. TUNEL method was used to detect the apoptosis of B16 malignant melanoma. RESULT: Antitumor assay in vitro showed that the absorbency increased in the concentration of 0.01, 0.001 mg x mL(-1) with statistical significance (P < 0.05 vs negative control). Antitumor assay in vivo showed that the tumor inhibitory rate of high dose (100 mg x kg(-1)) and middle dose (50 mg x kg(-1)) of total saponins of R. parvifolius were 37.02% and 30.61%, respectively. Loaded tumor mouse survival duration could be prolonged. The apoptosis indexes of B16 tumor cells in three treatment groups were 32.5%, 20.5% and 5.5%, respective and there was statistical significance (P < 0.05 vs negative control). CONCLUSION: The total saponins of R. parvifolius has remarkable inhibition of proliferation of malignant melanoma cells in vivo and in vitro and exerts antitumor activities through promoting tumor cell apoptosis.

[Dynamical examination of auditory event-related potentials P300 and sympathetic skin response in people with insomia of Sweet Dream Capsule therapy].

OBJECTIVE: To observe the change of auditory event-related potentials (P300) and sympathetic skin response (SSR) in people with insomia of Sweet Dream Capsule therapy. METHODS: 30 patients meeting criteria for primary insomnia and 30 healthy volunteers with age matching controls were selected for the study. P300 and SSR were measured before treatment of Sweet Dream Capsule and at week 4 , 8 of the therapeutic course. That the change of P300 and SSR before and after treatment and their relations with PSQI were studied. RESULT: Compared with those of normal controls, both P300 latency and SSR latency were prolonged while amplitude was decreased in patients with insomnia (P < 0.01). P300 amplitude was increased significantly at central (Cz) electrode sites only at week 8 when compared with amplitude before treatment (P < 0.05). With improvement of symptom and PSQI scores, latency and amplitude of SSR were improved at week 4 and week 8 (P < 0.05 and P < 0.01) . CONCLUSION: P300 has a some improvement in people with insomia of Sweet Dream Capsule therapy while SSR im proves significantly, and PSQI scores are ameliorated too.