ABSTRACT
The chemical components of Lycii Fructus were analyzed by liquid chromatography( LC) and mass spectrometry( MS for the establishment of spectrum-activity relationship,on the basis of which its antioxidant active ingredients were determined. In this experiment,Lycii Fructus was extracted with different solvents and then separated into 80 samples by macroporous adsorption resin and reversed-phase chromatography,respectively. The antioxidant components were enriched into 11 samples and their scavenging abilities against DPPH free radical and ferric ion reducing antioxidant power( FRAP) were significantly stronger than those before the treatment( P<0. 05). The spectrum-activity relationship regarding the antioxidant activity in vitro of Lycii Fructus was established by Pearson correlation analysis,orthogonal partial least squares( OPLS) and elastic net regression. Six chromatographic peaks greatly contributing to the antioxidant activity in vitro of Lycii Fructus were identified as rutin( P6),quercetin( P35),scopoletin( P14),N-cis-feruloyl-4-O-β-D-glucopyranosyl-tyramine or N-( 4-O-β-D-glucopyranosyl-trans-feruloyl)-tyramine( P8), ferulic acid( P13) and1,3,5-dihydroxy-2-isoprenyl-3-xanthone( P23). The active components associated with free radical scavenging were rutin and quercetin both belonging to flavonoids. The reduction of Fe3+was based on phenylpropanoids such as ferulic acid,scopoletin,xanthone and phenolic amides. These results indicated that the antioxidant activity of Lycii Fructus was ascribed to the synergistic action of different products through different ways. Besides,the data analysis model should be chosen carefully for the establishment of spectrum-activity relationship,thus ensuring the reliability of results.
Subject(s)
Antioxidants , Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Fruit , Phenols , Reproducibility of ResultsABSTRACT
One common feature of glaucoma, optic neuritis and some other optic nerve diseases is sustained and irreversible apoptosis of retinal ganglion cells (RGCs). Ginkgolide B is believed to protect neurons in brain and contribute to neurite outgrowth and synapse formation. The aim of the present study was to explore the effects of Ginkgo biloba extract (EGB761) and ginkgolide B on axonal growth of RCGs. Retina explants were cultured in three-dimensional tissue culture system, and the number and length of neurites were analyzed. Immunohistochemistry staining was performed to confirm that the neurite observed was axon of RGCs. TUNEL and activated caspase-3 staining were also applied to observe RGCs apoptosis. The result shows that neurites of RGCs treated with EGB761 or ginkgolide B were more and longer than those in control. The neurite is proved to be the axon of RGCs by immunostaining. Furthermore, compared with control group, RGCs treated with ginkgolide B showed decreased cellular apoptosis and inhibited caspase-3 activation. These results suggest ginkgolide B can promote RGCs axon growth by protecting RGCs against apoptosis.
Subject(s)
Animals , Rats , Apoptosis , Axons , Caspase 3 , Metabolism , Ginkgolides , Pharmacology , Lactones , Pharmacology , Neurites , Organ Culture Techniques , Plant Extracts , Pharmacology , Retina , Retinal Ganglion Cells , Cell BiologyABSTRACT
<p><b>OBJECTIVE</b>To probe the optimal acupuncture treatment program for juvenile simple obesity.</p><p><b>METHODS</b>Ninety cases were randomly divided into 3 groups: ear acupuncture group, body acupuncture group and control group. The patients of the control group were treated by regulating diet structure, increasing motion and mental direct of slimming, in the body acupuncture group, body acupuncture at Sanyinjiao (SP 6), Tianshu (ST 25), Zusanli (ST 36), etc. were added, and in the ear acupuncture group, acupuncture at ear acupoints Jidian (hunger point), Pizhixia (subcortex), earshenmen, etc. were added. Seventy days later, the therapeutic effects and changes of blood lipids were observed. And they were followed up after one month.</p><p><b>RESULTS</b>The total effective rate was 69.23% in the body acupuncture group, 65.52% in the ear acupuncture group and 26.67% in the control group, with significant differences as the two acupuncture groups compared with the control group. And there were significant differences in total cholesterol (TC) and lower density lipid protein-cholesterol (LDL-C) as the two acupuncture groups compared with the control group. There was no significant difference between the two acupuncture groups in all the indexes.</p><p><b>CONCLUSION</b>Both ear acupuncture and body acupuncture have obvious therapeutic effect on juvenile simple obesity, and have some actions on blood lipids.</p>
Subject(s)
Adolescent , Humans , Acupuncture , Acupuncture Therapy , Acupuncture, Ear , Lipids , Obesity , TherapeuticsABSTRACT
<p><b>OBJECTIVE</b>To study comparatively the effect of Guanxin II and its constituents, including Salvia, Red Peony root, Chuanxiong, Safflower and Dalbergia wood, in scavenging active oxygen free radical (OFR) to explore their mechanism in overcoming the experimental acute ischemic myocardial injury and protecting myocardial tissue.</p><p><b>METHODS</b>The experimental acute myocardial ischemic model was established by intraperitoneal injection of pituitrin to rats. OFR level in animal heart tissue was directly measured with low-temperature electron paramagnetic resonance (EPR) spectrometer.</p><p><b>RESULTS</b>The pathological examination of HE stained slide of myocardial tissue and electrocardiography of model animal showed that typical changes of acute myocardial ischemia occurred in myocardial tissue, EPR showed that OFR level in myocardial tissue increased abnormally. The ethanol extract of Guanxin II and its constituents could lower the increased OFR level close to normal, thus to alleviate the myocardial damage.</p><p><b>CONCLUSION</b>Overproduction of OFR could induce damage of heart tissue, its level could be measured directly using low temperature EPR. One of the molecular mechanisms of Guanxin II and its constituents in antagonizing and repairing myocardial damage is to scavenge the abnormal increased active OFR in tissue. This study has provided a basis for further studying the mechanism of Chinese composite recipes and their constituents.</p>