ABSTRACT
AIM: To study the anthraquinone constituents of the stem of Cassia siamea. METHODS: The compounds were isolated by chromatography on silica gel, MHPLC, and identified on the basis of spectral analysis including IR, EI-MS, FAB-MS, 1HNMR, 13CNMR and DEPT. RESULTS: Three compounds were isolated and identified as: chrysophanol (I), chrysophanol-1-O-beta-D-glucopyranoside (II) and 1-[(beta-D-glucopyranosyl-(1-->6)-0-beta-D-glucopyranosyl) oxy]-8-hydroxyl-3-methy-9,10-anthraquinone (III). CONCLUSION: III is a new compound, II was obtained from this plant for the first time.
Subject(s)
Anthraquinones/isolation & purification , Cassia/chemistry , Disaccharides/isolation & purification , Plants, Medicinal/chemistry , Anthraquinones/chemistry , Disaccharides/chemistry , Glucosides/chemistry , Glucosides/isolation & purification , Molecular Structure , Plant Stems/chemistryABSTRACT
In order to study the mechanism of the therapeutic effect of Chinese medicine in raising white blood cells, the effects of multiplication of splenocyte of leukopenic mice, the activity of interleukin-2 (IL-2), the lipoperoxidant (LPO) content, the concentration of blood serum testosterone of male mice by using Fuzheng Shengbai Capsules (FZSBC) were observed. The results showed that the multiplication of splenocyte, the activity of IL-2, and testosterone level of the experimental group were significantly higher than those of the control group (P < 0.05-0.01), LPO was decreased slightly, which had no statistic significance. Therefore, the increasing of WBC had no relation with the content of LPO but was positively correlated with T lymphocytes as well as the activity of IL-2. This study showed that the mechanism of leukocytogenic effect of FZSBC might be that it was related with promoting the proliferation of T lymphocytes, elevating the activity of IL-2 and raising the testosterone level, but was not relevant with anti-LPO.