[Microbial Diversity and Influencing Factors in a Small Watershed in Winter].

The water quality of a small watershed will directly affect the water quality safety of the entire watershed. The Lidu small watershed of the Fulin District in Chongqing was selected for this study. The characteristics of runoff and the microbial community in the receiving water body were analyzed by high-throughput sequencing technology. The effects of the physical and chemical factors of the water body on the microbial diversity in the water body were also investigated. The results showed that the small watershed of Lidu was an important source of nitrite and dissolved organic carbon for the receiving water. There was no significant difference in the microbial richness and diversity of the water flowing through different pollution sources in the small watershed, and the microbial diversity index was negatively correlated with the total phosphorus (r -0.79--0.84, P ≤ 0.01) and phosphate (r -0.71--0.80, P ≤ 0.01) of the water. At the phylum level, Actinobacteria (37.33% ±14.69%) accounted for the largest proportion of runoff flowing through the agricultural area, followed by Proteobacteria (32.53% ±7.18%), Cyanobacteria (14.65% ±22.64%), Bacteroidetes (8.50% ±3.67%), and others. Proteobacteria (43.67% ±10.80%) accounted for the largest proportion of runoff flowing through the industrial area, followed by Bacteroidetes (25.33% ±11.97%), Actinobacteria (24.17% ±14.66%), Firmicutes (2.53% ±0.72%), and others. At the genus level, hgcI_clade (19.08% ±13.46%) accounted for the largest proportion of runoff flowing through the agricultural area, followed by CL 500-29 _marine_group (7.40% ±4.44%), Limnohabitans (7.05% ±3.14%), and others. Flavobacterium (20.40% ±12.37%) accounted for the largest proportion of runoff flowing through the industrial area, followed by hgcI_clade (15.30% ±11.11%), Acinetobacter (9.33% ±11.50%), and others. The Flavobacterium in the runoff water may be related to the input of industrial sources in the watershed. Nitrous nitrogen, total phosphorus, phosphate, pH, and Zn2+ were the main environmental factors that affected the microbial community in the small watershed. They can explain the variance of microbial community 0.26, 0.21, 0.20, 0.15, and 0.14, respectively. DOC, nitrate nitrogen, and heavy metal ions such as Cu2+, Zn2+, Mn2+, and As3+ were also related to the proportion of some microorganisms in the water.

[Clinical observation on hailong juanxiao recipe combined with kechuanping mounting on yongquan acupoint in treating children' bronchial asthma in the stage of attack].

OBJECTIVE: To observe the effect of children' bronchial asthma in the stage of attack treated by Hailong Juanxiao Recipe combined with Kechuanping sticking on Yongquan acupoint (HKSYA). METHODS: One hundred and fifty children aging between 4-14 years old with bronchial asthma were randomly divided into HKSYA group (treated group, 75 cases) and Western medicine control group (control group, 75 cases). The clinical efficacy, eosinophilis (EOS) in peripheral blood and serum IgE of the patients were determined after 2 weeks of treatment. RESULTS: The clinical efficacy in the treated group was 89.3%, compared with the control group (64.0%), the difference was significant (P < 0.05). EOS and IgE in both groups after treatment were obviously lower with significant difference when compared with before treatment (P < 0.01 or P < 0.05). CONCLUSION: HKSYA could improve the clinical symptoms and the observation item, and enhance the clinical efficacy.