Effect of adding soluble viscous fibers to diets containing coarse and finely ground insoluble fibers on digesta transit behavior and nutrient digestibility in broiler chickens.

This paper aimed to study the interactive effects of the addition of soluble arabinoxylans (AX) and the particle size (PS) of soybean hulls (SBH) on digesta mean retention time (MRT) and nutrient digestibility in broiler chickens. A total of 288 one-day old Ross 308 female chicks were assigned to 32 pens (9 birds/pen) and fed a commercial starter diet for 10 d. At 10 d of age, pens were assigned to 1 of 4 dietary treatments (8 pens/diet) containing 120 g/Kg coarse or fine SBH, with or without addition of 50 g/Kg of soluble wheat AX, substituting maize starch. Titanium dioxide (4 g/Kg) and cobalt-EDTA (1 g/Kg) were added as inert markers. Excreta were quantitatively collected from d 22 to 25. Gastrointestinal tract and digesta were collected on d 28, 29, or 30. Arabinoxylans reduced the weight of the gizzard relative to body weight (RW) by 0.07% units (P = 0.005), and increased ceca RW (0.28 vs. 0.34%, P < 0.001) and length (10.45 vs. 11.21 cm/Kg BW, P < 0.001). Arabinoxylans increased digesta MRT in the crop (solids/liquids: +12 min, P < 0.05), small intestine (solids/liquids: +17 min, P < 0.01), and hindgut (liquids: +77.5 min, P < 0.05); and reduced apparent ileal digestibility (AID) and apparent total tract retention (ATTR) of DM (-5.4 and -3.9%, P < 0.001, respectively) and starch (-1.35 and -0.7%, P < 0.001, respectively). Particle size of SBH only affected the ATTR of non-starch polysaccharides, presenting higher retention values with fine SBH (-4.3%-units, P = 0.034). The addition of AX reduced AID of N by 4.3%-units, only in presence of fine SBH (interaction, P < 0.05). In conclusion, arabinoxylans greatly influenced digestion in the chicken GIT, while PS of SBH had marginal effects. Arabinoxylans reduced AID of N only with fine SBH, suggesting coarse SBH counteracted AX effects on N digestion, speculatively by modifying digesta viscosity.

Presence of Unabsorbed Free Amino Acids at the End of the Small Intestine Indicates the Potential for an Increase in Amino Acid Uptake in Humans and Pigs.

BACKGROUND: Unabsorbed free amino acids (AAs) at the end of the small intestine result in a potential preventable nutritional loss. OBJECTIVES: This study aimed to quantify free AAs in terminal ileal digesta of both humans and pigs to investigate its relevance for the nutritional value of food proteins. METHODS: Two studies with three diets were performed: a human study-ileal digesta from eight adult ileostomates were collected over 9 h after ingestion of a single meal unsupplemented or supplemented with 30 g zein or whey; pig study-12 cannulated pigs were fed for 7 d with a diet containing whey or zein or no-protein diet, and ileal digesta were collected on the last 2 d. Digesta were analyzed for total and 13 free AAs. True ileal digestibility (TID) of AAs was compared with and without free AAs. RESULTS: All terminal ileal digesta samples contained free AAs. The TID of AAs in whey was 97% ± 2.4% (mean ± SD) in human ileostomates and 97% ± 1.9% in growing pigs. If the analyzed free AAs would have been absorbed, TID of whey would increase by 0.4%-units in humans and 0.1%-units in pigs. The TID of AAs in zein was 70% ± 16.4% in humans and 77% ± 20.6% in pigs and would increase by 2.3%-units and 3.5%-units, respectively, if the analyzed free AAs would have been fully absorbed. The largest difference was observed for threonine from zein: if free threonine was absorbed, the TID would increase by 6.6%-units in both species (P < 0.05). CONCLUSIONS: Free AAs are present at the end of the small intestine and can potentially have a nutritionally relevant effect for poorly digestible protein sources, whereas the effect is negligible for highly digestible protein sources. This result provides insight into the room for improvement of a protein's nutritional value if all free AAs are to be absorbed. J Nutr 2023;xx:xx-xx. This trial was registered at clinicaltrials.gov as NCT04207372.

Reflux of 15N-labeled uric acid after intracloacal infusion in broiler chickens fed low- or high-protein diets.

Reflux of urine from the cloaca into the ceca provides chickens with a mechanism for recycling of urinary-Nitrogen (N) in a way analogous to urea recycling in mammals. However, it is unknown if reflux has substantial relevance in current poultry husbandry, where birds are fed ad libitum and have high protein intake. To evaluate the fate of urinary-N in ad libitum-fed broiler chickens, 15-day-old broilers were assigned to a high (21.9% CP, n = 22) or low (10.2 % CP, n = 22) protein diet. At 25 d of age, 20 broilers per dietary treatment were infused into the cloaca with a pulse dose of 107 mg [1,3-15N]-uric acid. N-contents and 15N-enrichment in digesta, blood plasma, and body tissues were measured at 5, 30, 60, 90, 150, 300, 450, 600, 1,200, or 1,800 min after administration (n = 2 /time-point /diet). Two broilers per dietary treatment were infused with saline and served as control to analyze background 15N-enrichment. The average total recovery (% of infused (w/w)) of 15N from infused uric acid in all body tissues was low (2.9 ± 0.62 %), of which the largest proportion was found in carcass tissue (2.5 ± 0.60%). 15N-enrichment was greatest in intestinal tissues. Even at 1,200 min, 15N-enrichment of ceca (0.46 ± 0.169 APE) and colon (0.13 ± 0.159 APE) digesta was considerably exceeding background enrichment. 15N-enrichment in excess of background enrichment in cecum and colon digesta (10-fold, P < 0.05), and 15N recovery in intestinal tissues (4-fold, P < 0.01) were greater in birds fed the low protein diet compared with the high protein diet, speculatively pointing out differences in the occurrence of reflux, incorporation of uric acid-N derivatives in intestinal tissues by first-pass metabolism, and a prolonged digesta retention time in protein deficient birds. In conclusion, these data confirm that uric acid-N infused in the cloaca can be refluxed and used for body N-deposition, but its contribution to whole body protein metabolism in broilers is probably limited.

Determination of True Ileal Amino Acid Digestibility in the Growing Pig for Calculation of Digestible Indispensable Amino Acid Score (DIAAS).

Digestible indispensable amino acid score (DIAAS) has been recommended by the FAO for the evaluation of protein quality in human foods, but the application of DIAAS is currently limited because of a lack of published data on the true ileal amino acid (AA) digestibility of AAs in foods. The importance of DIAAS is highlighted. To calculate DIAAS, it is necessary to determine the true ileal AA digestibility of human foods using the growing pig as an animal model for the human based on previous FAO recommendations. A method is described in detail in Supplemental Methods to determine the true ileal AA digestibility of foods for humans using the pig as a model for the adult human. Adoption of the method will enable consistency in the development of databases on predicted true ileal AA digestibility in human foods for the calculation of DIAAS.