ABSTRACT
Carbon (C) and nitrogen (N) metabolisms participate in N source-regulated secondary metabolism in medicinal plants, but the specific mechanisms involved remain to be investigated. By using nitrate (NN), ammonium (AN), urea (UN), and glycine (GN), respectively, as sole N sources, we found that N sources remarkably affected the contents of diterpenoid lactone components along with C and N metabolisms reprograming in Andrographis paniculata, as compared to NN, the other three N sources raised the levels of 14-deoxyandrographolide, andrographolide, dehydroandrographolide (except UN), and neoandrographolide (except AN) with a prominent accumulation of farnesyl pyrophosphate (FPP). These N sources also raised the photosynthetic rate and the levels of fructose and/or sucrose but reduced the activities of phosphofructokinase (PFK), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), phosphoenolpyruvate carboxylase (PEPC) and pyruvate dehydrogenase (PDH). Conversely, phosphoenolpyruvate carboxykinase (PEPCK) and malate enzyme (ME) activities were upregulated. Simultaneously, citrate, cis-aconitate and isocitrate levels declined, and N assimilation was inhibited. These results indicated that AN, UN and GN reduced the metabolic flow of carbohydrates from glycolysis into the TCA cycle and downstream N assimilation. Furthermore, they enhanced arginine and GABA metabolism, which increased C replenishment of the TCA cycle, and increased ethylene and salicylic acid (SA) levels. Thus, we proposed that the N sources reprogrammed C and N metabolism, attenuating the competition of N assimilation for C, and promoting the synthesis and accumulation of andrographolide through plant hormone signaling. To obtain a higher production of andrographolide in A. paniculata, AN fertilizer is recommended in its N management.
Subject(s)
Andrographis paniculata , Diterpenes , Plant Extracts , Carbon , SeedlingsABSTRACT
Understanding neural pathways and cognitive processes involved in the transformation of dietary fats into sensory experiences has profound implications for nutritional well-being. This study presents an efficient approach to comprehending the neural perception of fat taste using electroencephalogram (EEG). Through the examination of neural responses to different types of fatty acids (FAs) in 45 participants, we discerned distinct neural activation patterns associated with saturated versus unsaturated fatty acids. The spectrum analysis of averaged EEG signals revealed notable variations in δ and α-frequency bands across FA types. The topographical distribution and source localization results suggested that the brain encodes fat taste with specific activation timings in primary and secondary gustatory cortices. Saturated FAs elicited higher activation in cortical associated with emotion and reward processing. This electrophysiological evidence enhances our understanding of fundamental mechanisms behind fat perception, which is helpful for guiding strategies to manage hedonic eating and promote balanced fat consumption.
Subject(s)
Brain , Dietary Fats , Electroencephalography , Taste Perception , Humans , Female , Young Adult , Adult , Male , Brain/physiology , Dietary Fats/metabolism , Dietary Fats/analysis , Taste , Fatty Acids/chemistry , Fatty Acids/metabolismABSTRACT
Domestic wastewater source-separated treatment has attracted wide attention due to the efficiency improvement of sewage treatment systems, energy saving, resource reuse, and the construction and operation cost saving of pipeline networks. Nonetheless, the excess source-separated urine still demands further harmless treatment. Sequencing batch biofilm reactor (SBBR), a new type of composite biofilm reactor developed by filling different fillers into the sequential batch reactor (SBR) reactor, has higher pollutant removal performance and simpler operation and maintenance. However, the phosphorus removal ability of the SBBR filling with conventional fillers is still limited and needs further improvement. In this study, we developed two new fillers, the self-fabricated filler A and B (SFA/SFB), and compared their source-separated urine treatment performance. Long-term treatment experimental results demonstrated that the SBBR systems with different fillers had good removal performance on the COD and TN in the influent, and the removal rate increased with the increasing HRT. However, only the SBBR system with the SFA showed excellent PO43--P and TP removal performance, with the removal rates being 83.7 ± 11.9% and 77.3 ± 13.7% when the HRT was 1 d. Microbial community analysis results indicated that no special bacteria with strong phosphorus removal ability were present on the surface of the SFA. Adsorption experimental results suggested that the SFA had better adsorption performance for phosphorus than the SFB, but it could not always have stronger phosphorus adsorption and removal performance during long-term operation due to the adsorption saturation. Through a series of characterizations such as SEM, XRD, and BET, it was found that the SFA had a looser structure due to the use of different binder and production processes, and the magnesium in the SFA gradually released and reacted with PO43- and NH4+ in the source-separated urine to form dittmarite and struvite, thus achieving efficient phosphorus removal. This study provides a feasible manner for the efficient treatment of source-separated urine using the SBBR system with self-fabricated fillers.
Subject(s)
Magnesium , Waste Disposal, Fluid , Waste Disposal, Fluid/methods , Phosphorus , Bioreactors , Nitrogen , Excipients , Biofilms , Sewage/chemistryABSTRACT
Watershed water quality modeling is a valuable tool for managing ammonium (NH4+) pollution. However, simulating NH4+ pollution presents unique challenges due to the inherent instability of NH4+ in natural environment. This study modified the widely-used Soil and Water Assessment Tool (SWAT) model to simulate non-point source (NPS) NH4+ processes, specifically incorporating the simulation of land-to-water NH4+ delivery. The Jiulong River Watershed (JRW) is the study area, a coastal watershed in Southeast China with substantial sewage discharge, livestock farming, and fertilizer application. The results demonstrate that the modified model can effectively simulate the NPS NH4+ processes. It is recommended to use multiple sets of observations to calibrate NH4+ simulation to enhance model reliability. Despite constituting a minor proportion (5.6 %), point source inputs significantly contribute to NH4+ load at watershed outlet (32.4â¼51.9 %), while NPS inputs contribute 15.3â¼17.3 % of NH4+ loads. NH4+ primarily enters water through surface runoff and lateral flow, with negligible leaching. Average NH4+ land-to-water delivery rate is about 2.35 to 2.90 kg N/ha/a. High delivery rates mainly occur at agricultural areas. Notably, proposed NH4+ mitigation measures, including urban sewage treatment enhancement, livestock manure management improvement, and fertilizer application reduction, demonstrate potential to collectively reduce the NH4+ load at watershed outlet by 1/4 to 1/3 and significantly enhance water quality standard compliance frequency. Insights gained from modeling experience in the JRW offer valuable implications for NH4+ modeling and management in regions with similar climates and significant anthropogenic nitrogen inputs.
Subject(s)
Ammonium Compounds , Water Pollutants, Chemical , Fertilizers , Sewage , Reproducibility of Results , Environmental Monitoring/methods , Nitrogen/analysis , Water Quality , China , Rivers , Water Pollutants, Chemical/analysis , Phosphorus/analysisABSTRACT
Following a request from the European Commission, the EFSA Panel on Nutrition, Novel Foods and Food Allergens (NDA) was asked to deliver an opinion on the safety of magnesium l-threonate as a novel food (NF) pursuant to Regulation (EU) 2015/2283 and to address the bioavailability of magnesium from this source in the context of Directive 2002/46/EC. The NF, produced by chemical synthesis, is intended to be used as new source for magnesium in food supplements at a maximum intake level of 3000 mg per day by adults, except for pregnant and lactating women. This dose corresponds to ~ 2730 mg l-threonate and 250 mg magnesium, which also corresponds to the UL for supplemental magnesium from readily dissociable magnesium salts. Based on results obtained from a dissociation study, two rat studies and one human trial, the Panel considers that magnesium is bioavailable from the NF. The NF may contain up to 1% oxalic acid. The Panel considers that an additional exposure to oxalic acid, that is up to 30 mg daily from the NF, is not to be of safety concern. The Panel concludes that the NF is not nutritionally disadvantageous. In 2008, the EFSA ANS Panel concluded that a human intake of l-threonate of 2700 mg per day is safe. This intake is similar to the maximum intake of l-threonate from the NF under the maximum proposed uses, and the NDA Panel concurs with the ANS Panel that this intake is safe. The Panel considers that there are no concerns regarding the genotoxicity of the NF. The Panel concludes that the NF, Mg l-threonate, is safe under the proposed conditions of use. The Panel concludes that the NF is a source from which magnesium is bioavailable.
ABSTRACT
Cecropia is a genus of neotropical trees mainly distributed in Mexico and Central and South America. Currently, 63 species have been described, some of which have been used for centuries in traditional medicine to treat conditions such as diabetes, high blood pressure, and wound healing, among others. In recent times, modern phytochemical studies have succeeded in isolating individual compounds with potential specific medicinal applications. This review aims to examine the literature data regarding isolated terpenes and their correlation with pharmacological activities, with the goal of unveiling the future potential of the genus.
ABSTRACT
Phytonutrients (PTN) namely saponins (SP) and condensed tannins (CT) have been demonstrated to assess the effect of rumen fermentation and methane mitigation. Phytonutrient pellet containing mangosteen, rambutan, and banana flower (MARABAC) and lemongrass including PTN, hence these plant-phytonutrients supplementation could be an alternative plant with a positive effect on rumen fermentation. The aim of this experiment was to evaluate the effect of supplementation of MARABAC and lemongrass (Cymbopogon citratus) powder on in vitro fermentation modulation and the ability to mitigate methane production. The treatments were arranged according to a 3 × 3 Factorial arrangement in a completely randomized design. The two experimental factors consisted of MARABAC pellet levels (0%, 1%, and 2% of the total substrate) and lemongrass supplementation levels (0%, 1%, and 2% of the total substrate). The results of this study revealed that supplementation with MARABAC pellet and lemongrass powder significantly improved gas production kinetics (P < 0.01) and rumen fermentation end-products especially the propionate production (P < 0.01). While rumen methane production was subsequently reduced by both factors. Additionally, the in vitro dry matter degradability (IVDMD) and organic matter degradability (IVOMD) were greatly improved (P < 0.05) by the respective treatments. MARABAC pellet and lemongrass powder combination showed effective methane mitigation by enhancing rumen fermentation end-products especially the propionate concentration and both the IVDMD and IVOMD, while mitigated methane production. The combined level of both sources at 2% MARABAC pellet and 2% lemongrass powder of total substrates offered the best results. Therefore, MARABAC pellet and lemongrass powder supplementation could be used as an alternative source of phytonutrient in dietary ruminant.
Subject(s)
Cymbopogon , Dietary Supplements , Animals , Fermentation , In Vitro Techniques/veterinary , Methane/metabolism , Nutrients , Phytochemicals/metabolism , Powders/metabolism , Propionates/metabolism , Rumen/metabolismABSTRACT
Rapid technological development in agriculture and fast urbanization have increased nutrient losses in Europe. High nutrient export to seas causes coastal eutrophication and harmful algal blooms. This study aims to assess the river exports of nitrogen (N) and phosphorus (P), and identify required reductions to avoid coastal eutrophication in Europe under global change. We modelled nutrient export by 594 rivers in 2050 for a baseline scenario using the new MARINA-Nutrients model for Europe. Nutrient export to European seas is expected to increase by 13-28% under global change. Manure and fertilizers together contribute to river export of N by 35% in 2050. Sewage systems are responsible for 70% of future P export by rivers. By 2050, the top ten polluted rivers for N and P host 42% of the European population. Avoiding future coastal eutrophication requires over 47% less N and up to 77% less P exports by these polluted rivers.
Subject(s)
Environmental Monitoring , Eutrophication , Oceans and Seas , Rivers , Harmful Algal Bloom , Nitrogen/analysis , Phosphorus/analysis , Europe , NutrientsABSTRACT
Murrayae Folium et Cacumen (MFC) is a traditional Chinese medicine (TCM) derived from two plant species, Murraya exotica L. and Murraya paniculata (L.) Jack, as recorded in the Chinese Pharmacopoeia. However, there is no research available on the comprehensive analysis and comparison of the chemical constituents of these two species. In the present study, an integrated LC-MS-based quantitative metabolome strategy was proposed to conduct a comprehensive and in-depth qualitative and quantitative analysis and comparison of the chemome of M. exotica and M. paniculata. Firstly, the universal chemical information of two plants was obtained by quadrupole-time-of-flight mass spectrometry (Q-TOF-MS) combined with hybrid triple quadrupole-linear ion trap mass spectrometry (Qtrap-MS). Subsequently, a UNIFI in house database, the proposed fragmentation patterns, and a quantitative structure chromatographic retention relationship (QSRR) model were integrated for the rapid, comprehensive, and accurate structural elucidation of the chemical constituents of these two species. Thirdly, a large-scale quantitation method was established using scheduled multiple reaction monitoring mode (sMRM) and 76 primary components were selected as quantitative markers for the method validation. The obtained dataset was then subjected for multivariate statistical analysis to comprehensive comparison of these two plants. As a result, a total of 209 and 212 compounds were identified from M. exotica and M. paniculata, respectively. Among them, 103 common constituents were disclosed in both plants. The multivariate statistical analysis and absolute quantitative analysis revealed noticeable differences in the contents of specific chemical constituents between these two plants. The higher quantity constituents in M. exotica are 7-methoxycoumarins, while polymethoxylated flavonoids are the major constituents in M. paniculata. The common compounds accounted for approximately 80 % of the quantitative components in both plants, which provides a theoretical basis for their common use as the official source of MFC. In sum, the established quantitative chemomics strategy supplies an effective means for comprehensive chemical comparison of multi-source TCMs.
Subject(s)
Drugs, Chinese Herbal , Murraya , Murraya/chemistry , Mass Spectrometry , Chromatography, Liquid , Gas Chromatography-Mass Spectrometry/methods , Plant Extracts/chemistry , Chromatography, High Pressure Liquid/methods , Drugs, Chinese Herbal/chemistryABSTRACT
This study evaluated the impact of different dietary levels and sources of copper on the growth performance of nursery piglets through a combination of systematic review and meta-analysis. The database for this study was created using articles selected from major electronic databases. Data analysis involved forest plots and analysis of variance using mixed-effects models. The database included 63 articles published between 1990 and 2021, comprising 21,113 piglets in 946 treatments. Positive effects of supranutritional levels of copper from both inorganic and organic sources on the growth performance of nursery piglets were detected using Forest plots and analysis of variance (p < 0.001). Using mixed models, it was observed that piglet performance is influenced by body weight (p < 0.001), age (p < 0.001), and copper intake (p < 0.001). Both organic and inorganic sources of copper at supranutritional levels (>81 mg Cu/kg of diet) improved the performance of nursery piglets, but levels higher than 201 mg Cu/kg of diet did not further improve growth performance compared to 80-200 mg Cu/kg of diet. The feed conversion was worse in piglets fed with inorganic Cu sources (p < 0.001). In conclusion, dietary Cu supplementation influenced the weight gain and feed conversion rate in weaned piglets, particularly during the first few weeks post-weaning. Levels of 81 and 200 mg Cu/kg improved growth performance, but no further benefits were obtained for higher levels.
ABSTRACT
The global spread of antimicrobial resistance (AMR) in the environment is a growing health threat. Large rivers are of particular concern as they are highly impacted by wastewater discharge while being vital lifelines serving various human needs. A comprehensive understanding of occurrence, spread and key drivers of AMR along whole river courses is largely lacking. We provide a holistic approach by studying spatiotemporal patterns and hotspots of antibiotic resistance genes (ARGs) along 2311 km of the navigable Danube River, combining a longitudinal and temporal monitoring campaign. The integration of advanced faecal pollution diagnostics and environmental and chemical key parameters allowed linking ARG concentrations to the major pollution sources and explaining the observed patterns. Nine AMR markers, including genes conferring resistance to five different antibiotic classes of clinical and environmental relevance, and one integrase gene were determined by probe-based qPCR. All AMR targets could be quantified in Danube River water, with intI1 and sul1 being ubiquitously abundant, qnrS, tetM, blaTEM with intermediate abundance and blaOXA-48like, blaCTX-M-1 group, blaCTX-M-9 group and blaKPC genes with rare occurrence. Human faecal pollution from municipal wastewater discharges was the dominant factor shaping ARG patterns along the Danube River. Other significant correlations of specific ARGs were observed with discharge, certain metals and pesticides. In contrast, intI1 was not associated with wastewater but was already established in the water microbiome. Animal contamination was detected only sporadically and was correlated with ARGs only in the temporal sampling set. During temporal monitoring, an extraordinary hotspot was identified emphasizing the variability within natural waters. This study provides the first comprehensive baseline concentrations of ARGs in the Danube River and lays the foundation for monitoring future trends and evaluating potential reduction measures. The applided holistic approach proved to be a valuable methodological contribution towards a better understanding of the environmental occurrence of AMR.
Subject(s)
Genes, Bacterial , Rivers , Animals , Humans , Anti-Bacterial Agents/pharmacology , Anti-Bacterial Agents/analysis , Wastewater , Drug Resistance, Microbial/genetics , Water/analysisABSTRACT
Formaldehyde (HCHO) exposures during a full year were calculated for different race/ethnicity groups living in Southeast Texas using a chemical transport model tagged to track nine emission categories. Petroleum and industrial emissions were the largest anthropogenic sources of HCHO exposure in Southeast Texas, accounting for 44% of the total HCHO population exposure. Approximately 50% of the HCHO exposures associated with petroleum and industrial sources were directly emitted (primary), while the other 50% formed in the atmosphere (secondary) from precursor emissions of reactive compounds such as ethylene and propylene. Biogenic emissions also formed secondary HCHO that accounted for 11% of the total population-weighted exposure across the study domain. Off-road equipment contributed 3.7% to total population-weighted exposure in Houston, while natural gas combustion contributed 5% in Beaumont. Mobile sources accounted for 3.7% of the total HCHO population exposure, with less than 10% secondary contribution. Exposure disparity patterns changed with the location. Hispanic and Latino residents were exposed to HCHO concentrations +1.75% above average in Houston due to petroleum and industrial sources and natural gas sources. Black and African American residents in Beaumont were exposed to HCHO concentrations +7% above average due to petroleum and industrial sources, off-road equipment, and food cooking. Asian residents in Beaumont were exposed to HCHO concentrations that were +2.5% above average due to HCHO associated with petroleum and industrial sources, off-road vehicles, and food cooking. White residents were exposed to below average HCHO concentrations in all domains because their homes were located further from primary HCHO emission sources. Given the unique features of the exposure disparities in each region, tailored solutions should be developed by local stakeholders. Potential options to consider in the development of those solutions include modifying processes to reduce emissions, installing control equipment to capture emissions, or increasing the distance between industrial sources and residential neighborhoods.
Subject(s)
Air Pollutants , Formaldehyde/adverse effects , Petroleum , Respiratory Hypersensitivity , Air Pollutants/analysis , Vehicle Emissions/analysis , Texas , Natural Gas , Environmental Monitoring , Formaldehyde/analysisABSTRACT
Procedures such as transport and marketing can subject animals to water and feed deprivation and impair animal health and performance. Maintaining the mineral status of animals under these conditions can bring benefits to health and performance. The use of hydroxychloride mineral sources can improve mineral status, nutrient digestibility and performance. Two studies were conducted to investigate how the supplementation of 02 trace mineral sources of Cu and Zn and 48-hour water/feed deprivation would affect the performance and metabolism of grass-fed beef cattle. In the first study, 20 castrated and rumen-canulated Nellore steers (BWâ¯=â¯350⯱â¯132â¯kg; 20â¯m) were distributed in individual pens, in a 2â¯×â¯2 factorial arrangement: supplemental Cu and Zn sources from inorganic vs hydroxychloride (HTM) and 48-hours deprivation (WFD) vs unrestricted (WFU) access to water and feed. The 57d of study was divided into two periods: (1) Adaptation from -21d to -1d and (2) evaluation from 0d to 36d. Interaction between deprivationâ¯×â¯period was detected (Pâ¯<â¯0.05) for digestibility of DM (DMD), organic matter (OMD), neutral detergent fiber (NDFD), and acid detergent fiber (ADFD). Deprivation increased DMD, OMD, NDFD, and ADFD immediately after the deprivation period (3-5d), but impaired digestibility at longer periods such as 11-13d and 32-34d. DM (DMI) and nutrient intake (Pâ¯=â¯0.075), as well as NDFD were higher in HTM. Several ruminal parameters were affected by deprivation: short-chain fatty acids concentration decreased, while rumen pH increased (deprivationâ¯×â¯time; Pâ¯<â¯0.05); decreased propionate, butyrate and increased isobutyrate, isovalerate, and valerate in WFD (deprivationâ¯×â¯time; Pâ¯<â¯0.05), respectively. In the second study, eighty-four intact Nellore males (BWâ¯=â¯260⯱â¯35â¯kg) were blocked by BW and randomly assigned to Urochloa brizantha cv. Marandu paddocks for 131d in a 2â¯×â¯2 factorial arrangement. Liver Cu was higher in WFU/HTM animals (mineralâ¯×â¯deprivation; Pâ¯<â¯0.05). Interaction between deprivationâ¯×â¯period (Pâ¯<â¯0.05) was detected for BW and average daily gain (ADG). On 2d and 12d after deprivation, WFD increased ADG and recovered the BW lost. In conclusion, water and feed deprivation imposed in these trials were able to impact several nutrient digestibility and ruminal fermentation parameters in short- and long-term. Performance was not affected by the studied factors. Furthermore, supplementation with sources of Cu and Zn hydroxychloride increased Cu in the liver and tended to increase DMI and NDFD.
Subject(s)
Copper , Zinc , Male , Cattle , Animals , Dietary Supplements , Diet/veterinary , Water/metabolism , Detergents/metabolism , Digestion , Minerals/metabolism , Fermentation , Animal Feed/analysis , Rumen/metabolismABSTRACT
By integrating soil and water assessment tool (SWAT) modeling and land use and land cover (LULC) based multi-variable statistical analysis, this study aimed to identify driving factors, potential thresholds, and critical source areas (CSAs) to enhance water quality in southern Alabama and northwest Florida's Choctawhatchee Watershed. The results revealed the significance of forest cover and of the lumped developed areas and cultivated crops ("Source Areas") in influencing water quality. The stepwise linear regression analysis based on self-organizing maps (SOMs) showed that a negative correlation between forest percent cover and total nitrogen (TN), organic nitrogen (ORGN), and organic phosphorus (ORGP), highlighting the importance of forests in reducing nutrient loads. Conversely, Source Area percentage was positively correlated with total phosphorus (TP) loads, indicating the influence of human activities on TP levels. The receiver operating characteristic (ROC) curve analysis determined thresholds for forest percentage and Source Area percentage as 37.47 % and 20.26 %, respectively. These thresholds serve as important reference points for identifying CSAs. The CSAs identified based on these thresholds covered a relatively small portion (28 %) but contributed 47 % of TN and 50 % of TP of the whole watershed. The study underscores the importance of considering both physical process-based modeling and multi-variable statistical analysis for a comprehensive understanding of watershed management, i.e., the identification of CSAs and the associated variables and their tipping points to maintain water quality.
Subject(s)
Non-Point Source Pollution , Water Pollutants, Chemical , Humans , Water Quality , Soil , Non-Point Source Pollution/analysis , Environmental Monitoring , Water Pollutants, Chemical/analysis , Rivers , Phosphorus/analysis , Nitrogen/analysis , ChinaABSTRACT
Phosphorus (P) is a pivotal element responsible for triggering watershed eutrophication, and accurate source apportionment is a prerequisite for achieving the targeted prevention and control of P pollution. Current research predominantly emphasizes the allocation of total phosphorus (TP) loads from watershed pollution sources, with limited integration of source apportionment considering P species and their specific implications for eutrophication. This article conducts a retrospective analysis of the current state of research on watershed P source apportionment models, providing a comprehensive evaluation of three source apportionment methods, inventory analysis, diffusion models, and receptor models. Furthermore, a quantitative analysis of the impact of P species on watersheds is carried out, followed by the relationship between P species and the P source apportionment being critically clarified within watersheds. The study reveals that the impact of P on watershed eutrophication is highly dependent on P species, rather than absolute concentration of TP. Current research overlooking P species composition of pollution sources may render the acquired results of source apportionment incapable of assessing the impact of P sources on eutrophication accurately. In order to enhance the accuracy of watershed P pollution source apportionment, the following prospectives are recommended: (1) quantifying the P species composition of typical pollution sources; (2) revealing the mechanisms governing the migration and transformation of P species in watersheds; (3) expanding the application of traditional models and introducing novel methods to achieve quantitative source apportionment specifically for P species. Conducting source apportionment of specific species within a watershed contributes to a deeper understanding of P migration and transformation, enhancing the precise of management of P pollution sources and facilitating the targeted recovery of P resources.
Subject(s)
Environmental Monitoring , Water Pollutants, Chemical , Environmental Monitoring/methods , Phosphorus/analysis , Retrospective Studies , Rivers , Water Quality , China , Nitrogen/analysis , Water Pollutants, Chemical/analysisABSTRACT
The objective of this study was to determine how different sources of Zn, Mn, and Cu in the feed without and with phytase affect prececal myo-inositol hexakisphosphate (InsP6) breakdown to myo-inositol (MI), prececal P digestibility, bone mineralization, and expression of mineral transporters in the jejunum of broiler chickens. A total of 896 male broiler chicks (Cobb 500) were distributed to 7 diets with 8 replicate pens (16 birds per floor pen). Experimental diets were fed from day 0 to 28. Diets were without or with phytase supplementation (0 or 750 FTU/kg) and were supplemented with three different trace mineral sources (TMS: sulfates, oxides, or chelates) containing 100 mg/kg Zn, 100 mg/kg Mn, and 125 mg/kg Cu. Prececal InsP6 disappearance and P digestibility were affected by interaction (phytase × TMS: P ≤ 0.010). In diets without phytase supplementation, prececal InsP6 disappearance and P digestibility were greater (P ≤ 0.001) in birds fed chelated minerals than in birds fed sulfates or oxides. However, no differences were observed between TMS in diets with phytase supplementation. Ileal MI concentration was increased by exogenous phytase but differed depending on TMS (phytase × TMS: P ≤ 0.050). Tibia ash concentration as well as Zn and Mn concentration in tibia ash were increased by phytase supplementation (P < 0.010), but the Cu concentration in tibia ash was not (P > 0.050). Gene expression of the assayed mineral transporters in the jejunum was not affected by diet (P > 0.050), except for Zn transporter 5 (phytase × TMS: P = 0.024). In conclusion, the tested TMS had minor effects on endogenous phytate degradation in the digestive tract of broiler chickens. However, in phytase-supplemented diets, the choice of TMS was not relevant to phytate degradation under the conditions of this study.
ABSTRACT
Objective. Electroencephalograms (EEGs) are often used to monitor brain activity. Several source localization methods have been proposed to estimate the location of brain activity corresponding to EEG readings. However, only a few studies evaluated source localization accuracy from measured EEG using personalized head models in a millimeter resolution. In this study, based on a volume conductor analysis of a high-resolution personalized human head model constructed from magnetic resonance images, a finite difference method was used to solve the forward problem and to reconstruct the field distribution.Approach. We used a personalized segmentation-free head model developed using machine learning techniques, in which the abrupt change of electrical conductivity occurred at the tissue interface is suppressed. Using this model, a smooth field distribution was obtained to address the forward problem. Next, multi-dipole fitting was conducted using EEG measurements for each subject (N= 10 male subjects, age: 22.5 ± 0.5), and the source location and electric field distribution were estimated.Main results.For measured somatosensory evoked potential for electrostimulation to the wrist, a multi-dipole model with lead field matrix computed with the volume conductor model was found to be superior than a single dipole model when using personalized segmentation-free models (6/10). The correlation coefficient between measured and estimated scalp potentials was 0.89 for segmentation-free head models and 0.71 for conventional segmented models. The proposed method is straightforward model development and comparable localization difference of the maximum electric field from the target wrist reported using fMR (i.e. 16.4 ± 5.2 mm) in previous study. For comparison, DUNEuro based on sLORETA was (EEG: 17.0 ± 4.0 mm). In addition, somatosensory evoked magnetic fields obtained by Magnetoencephalography was 25.3 ± 8.5 mm using three-layer sphere and sLORETA.Significance. For measured EEG signals, our procedures using personalized head models demonstrated that effective localization of the somatosensory cortex, which is located in a non-shallower cortex region. This method may be potentially applied for imaging brain activity located in other non-shallow regions.
Subject(s)
Brain Mapping , Electroencephalography , Male , Humans , Young Adult , Adult , Brain Mapping/methods , Electroencephalography/methods , Magnetoencephalography/methods , Magnetic Resonance Imaging , Scalp , Brain/diagnostic imaging , Brain/physiology , Models, Neurological , Head/diagnostic imaging , Head/physiologyABSTRACT
Initial flush management is an effective measure to control non-point source pollution (NPSP) in storm runoff. However, determining the parameter of the initial flush in different areas may pose challenges in storm runoff management strategies. To address this issue, Erhai Lake in China, Yunnan-Guizhou Plateau, was selected as an example for the study. Erhai Lake is a typical mesotrophic lake with the profound influence of NPSP. The NPSP control strategy in this area will provide a valuable reference for other lakes. In 2021, 289 storm events and 190 ditchwater samples were detected around Erhai Lake. The average flow in the ditches ranged from 0.004 to 0.147 m3/s, the instant total nitrogen (TN) concentration ranged from 0.28 to 91.43 mg/L, and the instant total phosphorus (TP) concentration ranged from 0.26 to 7.35 mg/L in the storm events. It was found that the concentration of pollutants was lower than expected in the initial flush period. Instead, the event mean concentrations of TN and TP were 9.3 and 2.1 times higher than in the wet seasons, showing high nutrient concentration levels throughout the entire rainfall period. To manage storm runoff effectively, a flow-processes-division method was proposed to analyze the inflow condition and pollutant removal rate in different runoff periods. The peak flow interception strategy was recommended as the optimal stormwater management plan, as it showed the highest inflow conditions and 50% pollutant removal rate. Considering the need to reduce the constant flush of stormwater runoff, it is essential to establish a healthy water cycle system to alleviate NPSP and raise the Erhai water level. The storm runoff management method can serve as a practical tool for lake areas that do not exhibit initial flush characteristics.
Subject(s)
Environmental Pollutants , Water Pollutants, Chemical , Environmental Monitoring/methods , Water Pollutants, Chemical/analysis , Rain , China , Water Movements , Phosphorus , Nitrogen/analysisABSTRACT
KEY MESSAGE: The weighted gene co-expression network analysis and antisense oligonucleotide-mediated transient gene silencing revealed that CsAAP6 plays an important role in amino acid transport during tea shoot development. Nitrogen transport from source to sink is crucial for tea shoot growth and quality formation. Amino acid represents the major transport form of reduced nitrogen in the phloem between source and sink, but the molecular mechanism of amino acid transport from source leaves to new shoots is not yet clear. Therefore, the composition of metabolites in phloem exudates collected by the EDTA-facilitated method was analyzed through widely targeted metabolomics. A total of 326 metabolites were identified in the phloem exudates with the richest variety of amino acids and their derivatives (93), accounting for approximately 39.13% of the total metabolites. Moreover, through targeted metabolomics, it was found that the content of glutamine, glutamic acid, and theanine was the most abundant, and gradually increased with the development of new shoots. Meanwhile, transcriptome analysis suggested that the expression of amino acid transport genes changed significantly. The WGCNA analysis identified that the expression levels of CsAVT1, CsLHTL8, and CsAAP6 genes located in the MEterquoise module were positively correlated with the content of amino acids such as glutamine, glutamic acid, and theanine in phloem exudates. Reducing the CsAAP6 in mature leaves resulted in a significant decrease in the content of glutamic acid, aspartic acid, alanine, leucine, asparagine, glutamine, and arginine in the phloem exudates, indicating that CsAAP6 played an important role in the source to sink transport of amino acids in the phloem. The research results will provide the theoretical basis and genetic resources for the improvement of nitrogen use efficiency and tea quality.
Subject(s)
Amino Acids , Glutamine , Amino Acids/metabolism , Glutamates/metabolism , Tea , Gene Expression Profiling , Nitrogen/metabolismABSTRACT
In Kenya 26% of children under age 5 experience stunted growth, 4% are wasted and 11% are underweight. In pregnant women, the prevalence of iron deficiency is 36% and iron-deficiency anaemia prevalence is 26%. Previous studies have identified affordability as a key barrier to the intake of nutrients, particularly from animal-source foods (ASFs). Thus, this study analyzes to what extent the affordability of ASF in Kenya can be improved. It focuses on four ASFs: eggs, milk, chicken and beef. Using a computable general equilibrium model, three policy simulations were undertaken to establish the impact of potential changes on nutritious ASF availability and affordability: a 20% increase in total factor productivity (TFP) for the four products; a 20% TFP increase plus a 25% reduction in trade and transportation margins; and a 20% TFP increase for ASF and maize (a key input in animal feed). Simulations suggest increasing the productivity of the four ASF products would increase their availability and lower consumer prices (up to 17% lower). Household consumption of the four commodities would increase, resulting in improved household dietary diversity. Rural households would gain more compared with urban households. Poor households (the lowest 40%) would register larger welfare (Equivalent Variation) gains than other households in both urban and rural areas. The richest 20% of the population would neither lose nor gain following the policy changes. Reducing transportation costs and trade margins and increasing maize productivity could further reduce the price of ASFs through lower production costs and increased consumption.