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Mol Biotechnol ; 61(9): 674-680, 2019 Sep.
Article in English | MEDLINE | ID: mdl-31218651

ABSTRACT

The use of triclosan (TCS), an antimicrobial agent in consumer product, results in adverse effects on the environment due to its wide usage all over the world. The present study focused on TCS detection and attempted for degradation by biosurfactant produced by Bacillus licheniformis from domestic wastewater in Surathkal region, Karnataka, India. The experimental investigation includes biosurfactant production using crude sunflower oil and detection and degradation of TCS from wastewater by High-Performance Liquid Chromatography (HPLC). Results exhibited that maximum biosurfactant yield (7.8 g/L) was achieved using 1 g/L of glycerol as carbon and 5.5 g/L of ammonium bicarbonate as a nitrogen source. Detection of TCS from domestic wastewater (0.36 mg/L) and degradation was carried out by HPLC. The result discloses that 47.2% and 100% removal of TCS was achieved in 2 h and 16 h for 1:1(v/v) ratio of wastewater and biosurfactant.


Subject(s)
Bacillus licheniformis/chemistry , Surface-Active Agents/chemistry , Triclosan/metabolism , Wastewater/chemistry , Water Pollutants, Chemical/metabolism , Bacillus licheniformis/metabolism , Bicarbonates/metabolism , Biodegradation, Environmental , Chromatography, High Pressure Liquid , Glycerol/metabolism , Humans , Sunflower Oil/metabolism , Surface-Active Agents/isolation & purification , Surface-Active Agents/metabolism , Triclosan/chemistry , Water Pollutants, Chemical/chemistry
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