Research Note: In ovo and in-feed probiotic supplementation improves layer embryo and pullet growth.

Probiotics are widely used as feed supplements in the poultry industry to promote growth and performance in chickens. Specifically, this supplementation starts around the time of lay and continues through the production cycle in laying hens. However, the embryonic period is critical to the growth and development of metabolically active organs thereby influencing subsequent health and productivity in adult birds. Therefore, the present study investigated the potential use of probiotics to promote embryonic growth in layers. Further, a pilot grow-out study was conducted to evaluate the effect of in ovo and in-feed probiotic application on pullet growth. For the study, fertile White Leghorn eggs were sprayed with phosphate buffered saline (control, CON) or probiotic cocktail (in ovo only, IO; Lactobacillus paracasei DUP 13076 and L. rhamnosus NRRL B 442) prior to and during incubation. The embryos were sacrificed on d 7, 10, 14, and 18 of incubation for embryo morphometry. On d 18, remaining eggs were set in the hatcher to assess hatchability and hatchling morphometry. For the pullet trial, hatchlings were raised on feed with or without probiotics until wk 5. Pullets were sacrificed weekly, and morphometric parameters were recorded. Results of our study demonstrate that in ovo probiotic application significantly improved relative embryo weight, crown-rump length, hatchability, and hatchling weight when compared to the control (P < 0.05). Further, this enhanced embryonic development was associated with a concomitant increase in posthatch growth. Specifically, pullets raised from probiotic-sprayed eggs had significantly improved crown-rump length, tibial length, tibial bone weight, and body weight when compared to the control (P < 0.05). Moreover, among the different treatment schemes employed in this study [CON (no probiotics), in-feed only (IF), IO only, and in ovo and in-feed probiotic supplementation (IOIF)], sustained probiotic supplementation (IOIF) was found to be the most effective in promoting growth. Therefore, in ovo and in-feed probiotic supplementation could be employed to promote embryo and pullet growth to support subsequent performance in layers.

Graded levels of Eimeria challenge altered the microstructural architecture and reduced the cortical bone growth of femur of Hy-Line W-36 pullets at early stage of growth (0-6 wk of age).

An experiment was carried out to evaluate the impact of mixed Eimeria challenge on skeletal health of Hy-Line W-36 pullets. A total of 540, 16-day-old pullets were randomly allocated into 5 treatment groups, including a nonchallenged control. A mixed Eimeria species solution containing 50,000 E. maxima, 50,000 E. tenella, and 250,000 E. acervulina oocysts per mL was prepared and challenged to 1 group as a high-dose treatment. The 2-fold serial dilution was done to prepare the medium-high (25,000 E. maxima; 25,000 E. tenella; 125,000 E. acervulina), the medium-low (12,500 E. maxima; 12,500 E. tenella; 62,500 E. acervulina), and the low (6,250 E. maxima; 6,250 E. tenella; 31,250 E. acervulina) dose treatments which were challenged to 3 corresponding groups, respectively. The mineral apposition rate (MAR) was measured from 0 to 14 d post inoculation (DPI) and 14 to 28 DPI using calcein injection. The microstructural architecture of the femur was analyzed using the Skyscan X-ray microtomography (microCT) on 6, 14, and 28 DPI. The results showed that the MAR decreased linearly with an increase in the challenged dose (P < 0.05) during 0 to 14 DPI. The results of microCT revealed that cortical and total BMD, BMC, bone volume (BV), and bone volume as a fraction of tissue volume (BV/TV) of femur decreased both linearly (P < 0.05). Conversely, the total number of pores increased linearly with an increase in challenge dosages on 6 and 14 DPI. Trabecular BMD, BV, BV/TV, trabecular number, and trabecular thickness decreased linearly with an increase in the challenge dosages (P < 0.05) on 6 DPI. Furthermore, Eimeria infection significantly increased the number of osteoclasts and osteoclastic activity (P = 0.001). The result of this study suggests that the mixed Eimeria challenge negatively impacts the quality of skeletal health in a linear or quadratic manner with an increase in the concentration of Eimeria oocysts. The negative impact on long bone development might be due to malabsorption, nutrient deficiency during the infection, along with oxidative stress/inflammation disrupting the balance of osteoblastic and osteoclastic cells and their functions.

Effects of dietary protein levels on performance, nitrogen excretion, and odor emission of growing pullets and laying hens.

The objective of this study was to determine the effects of dietary crude protein (CP) levels on production performance, nitrogen balance, and odor emission of excreta in growing pullets and laying hens from 13 to 32 wk of age. Two hundred and forty pullets (Hy-Line Brown) were randomly assigned to 1 of 4 dietary groups with 10 replicates per group, and 6 birds per replicate. Experimental diets were formulated to contain 4 graded CP levels in the diets of pullets ranging from 180, 160, 140, and 120 g/kg of diet during 13 to 18 wk (phase 1) and in the diets of laying hens from 190, 170, 150, and 130 g/kg of diet during 19 to 32 wk (phase 2). The limiting amino acids including lysine, methionine, and threonine were supplemented to maintain constant equal amino acid concentrations in all experiment diets. In phase 1, decreasing dietary CP levels did not affect growth performance but increased (linear and quadratic effect, P < 0.05) the relative abdominal fat contents and triglyceride concentration in serum samples. High-density lipoprotein cholesterol in serum samples decreased as the CP levels decreased in the diets of pullets. Dietary CP levels quadratically increased (P < 0.05) the villus height and the villus height to crypt depth ratio but did not affect tibia traits and relative organ weights in pullets at 18 wk. Apparent digestibility of dry matter and ether extract increased with decreasing dietary CP levels in pullets. Graded CP levels linearly increased the digestibility of dry matter, CP, and ether extracts but lowered that of crude ash in laying hens. Nitrogen excretion was linearly decreased (P < 0.05) as the dietary CP levels decreased in both pullets and laying hens. Dietary CP levels only affected carbon dioxide emission in pullets. In phase 2, dietary CP levels did not affect growth performance and the ages at first egg laying and to reach 50% egg production in laying hens. However, egg weights were decreased (linear and quadratic effect, P < 0.05) as the dietary CP level decreased in laying hens. Increasing dietary CP levels increased Haugh unit at 26 wk but lowered corticosterone concentrations in yolk samples at 22 wk. Collectively, this study shows that dietary CP levels could be decreased to reduce nitrogen excretion without adverse effects on performance and egg quality of growing pullets and laying hens.

Long-term effects of dietary calcium and phosphorus level, and feed form during rearing on egg production, eggshell quality, and bone traits in brown laying hens from 30 to 89 wk of age.

The effects of feeding strategies during rearing (0-16 wk) of brown laying hens on mid and end laying performance (30-89 wk) were studied. The rearing feeding strategies followed a 3 × 2 factorial arrangement with feed form; mash with inclusion of 3% finely ground wheat straw (MWS), crumbles with inclusion of 3% finely ground wheat straw (CWS), and crumbles with inclusion of 3% unground oat hulls as fiber sources (COH) at 2 dietary Ca and P levels (high or low Ca-P). Feed conversion ratio improved with COH and MWS compared with CWS from 30 to 59 wk. Rate of lay and egg mass production showed a feed form × Ca-P interaction from 60 to 89 wk. Low Ca-P led to a higher egg production, but only when COH and MWS were fed. BW at 89 wk was higher with CWS compared to COH and MWS. BW uniformity was better with COH compared to MWS at 51 wk and both CWS and MWS at 67 wk. Tibia characteristics were not clearly affected by treatment, although there was a feed form × Ca-P interaction on compression at 89 wk, where compression was lower with MWS and low vs. high Ca-P. Low Ca-P during rearing led to higher eggshell thickness, compared to high Ca-P at 45 wk of age, but breaking strength was lower with low vs. high Ca-P at 75 wk. Although eggshell quality was affected by Ca-P and there were some interactions with feed form at some ages, the effect was not consistent. There was no clear relationship between eggshell quality and tibia characteristics. It was concluded that feeding low Ca-P in combination with COH and MWS during the rearing period positively affects egg production during late lay. Also, dietary Ca-P levels, compared to commercial practice, can be lowered during rearing, as this will not affect eggshell quality and bone mineralization at later ages.

Welfare issues and potential solutions for laying hens in free range and organic production systems: A review based on literature and interviews.

In free-range and organic production systems, hens can make choices according to their needs and desires, which is in accordance with welfare definitions. Nonetheless, health and behavioral problems are also encountered in these systems. The aim of this article was to identify welfare challenges observed in these production systems in the EU and the most promising solutions to overcome these challenges. It is based on a review of published literature and research projects complemented by interviews with experts. We selected EU specific information for welfare problems, however, the selected literature regarding solutions is global. Free range use may increase the risk of infection by some bacteria, viruses and parasites. Preventive methods include avoiding contamination thanks to biosecurity measures and strengthening animals' natural defenses against these diseases which can be based on nutritional means with new diet components such as insect-derived products, probiotics and prebiotics. Phytotherapy and aromatherapy can be used as preventive and curative medicine and vaccines as alternatives to antibiotics and pesticides. Bone quality in pullets and hens prevents keel deviations and is favored by exercise in the outdoor range. Free range use also lead to higher exposure to variable weather conditions and predators, therefore shadow, fences and guard animals can be used to prevent heat stress and predation respectively. Granting a free range provides opportunities for the expression of many behaviors and yet many hens usually stay close to the house. Providing the birds with trees, shelters or attractive plants can increase range use. Small flock sizes, early experiences of enrichment and personality traits have also been found to enhance range use. Severe feather pecking can occur in free range production systems, although flocks using the outdoor area have better plumage than indoors. While many prevention strategies are facilitated in free range systems, the influence of genetics, prenatal and nutritional factors in free range hens still need to be investigated. This review provides information about practices that have been tested or still need to be explored and this information can be used by stakeholders and researchers to help them evaluate the applicability of these solutions for welfare improvement.

Effect of chlorogenic acid on intestinal inflammation, antioxidant status, and microbial community of young hens challenged with acute heat stress.

Heat stress in poultry is deleterious to productive performance. Chlorogenic acid (CGA) exerts antibacterial, anti-inflammatory, and antioxidant properties. This study was conducted to evaluate the effects of dietary supplemental CGA on the intestinal health and cecal microbiota composition of young hens challenged with acute heat stress. 100-day-old Hy-line brown pullets were randomly divided into four groups. The control group (C) and heat stress group (HS) received a basal diet. HS + CGA300 group and HS + CGA600 group received a basal diet supplemented with 300- and 600-mg/kg CGA, respectively, for 2 weeks before heat stress exposure. Pullets of HS, HS + CGA300 , and HS + CGA600 group were exposed to 38°C for 4 h while the control group was maintained at 25°C. In this study, dietary CGA supplementation had effect on mitigate the decreased T-AOC and T-SOD activities and the increasing of IL-1ß and TNFα induced by acute heat stress. Dietary supplementation with 600 mg/kg CGA had better effect on increasing the relative abundance of beneficial bacterial genera, such as Rikenellaceae RC9_gut_group, Ruminococcaceae UCG-005, and Christensenellaceae R-7_group, and deceasing bacteria genera involved in inflammation, such as Sutterella species. Therefore, CGA can ameliorate acute heat stress damage through suppressing inflammation and improved antioxidant capacity and cecal microbiota composition.

The effects of dietary calcium and phosphorus level, and feed form during rearing on growth performance, bone traits and egg production in brown egg-type pullets from 0 to 32 weeks of age.

In a 3 × 2 factorial arrangement, effects of feed form (crumbles (CWS), mash (MWS), both with inclusion of 3% finely ground wheat straw, or crumbles with inclusion of 3% oat hulls (COH)), and dietary Ca and P (high and low Ca-P) from 0 to 16 wk of age were studied on growth performance, bone characteristics, and gizzard development of egg-type pullets. The cross-over effect of feeding strategy during rearing on laying performance and egg shell quality was studied from 19 to 32 wk of age. From 0 to 16 wk, ADG, ADFI, and feed conversion ratio (FCR) were improved with CWS and COH compared to MWS, but ADG and FCR were improved with MWS compared to CWS and COH from 11 to 16 wk. Uniformity of BW till 11 wk, and tibia breaking strength at 6 and 16 wk were higher with CWS and COH compared to MWS. Tibia ash content at 11 wk and relative empty proventriculus + gizzard weight (EPG) were lower with CWS and COH compared to MWS, also relative EPG at 11 and 16 wk was higher with COH compared to CWS. At 25 wk BW was lower with MWS compared to CWS and COH, but BW was equal for all treatments at 32 wk. The FCR for egg production was improved with COH compared to MWS. Egg shell parameters were not affected by feed form during rearing. Low Ca-P decreased BW uniformity at 6 wk, relative keel bone weight and ash content at 11 wk, tibia ash content at 11 and 16 wk, increased relative EPG at 6 wk, and improved egg shell quality at 32 wk of age. It was concluded that feeding CWS and COH compared to MWS increased growth performance, but had no clear cross-over effect on egg production. Low dietary Ca-P led to a lower bone mineralization during rearing, nevertheless improved egg shell quality at 32 wk.

Role of long-term supplementation of 25-hydroxyvitamin D3 on laying hen bone 3-dimensional structural development.

Egg-laying hens have a unique bone development pattern due to the medullary bone formation and high bone turnover rate. The role of long-term supplementation of an intermediate form of vitamin D3, 25-hydroxyvitamin D3 (25OHD), on skeletal development of pullets and laying hens is not well established. Exploring its effects on layer bone development will help develop a strategy for preventing laying hen osteoporosis. The purpose of this study was to investigate the role of long-term supplementation of 25OHD in layer diets on bone 3-dimensional structural development. A total of 390 1-day-old Hy-Line W36 pullets were randomly allocated to 3 treatments with 10 replicate cages and 13 birds/cage. Dietary treatments were 1) vitamin D3 at 2,760 IU/kg, 2) vitamin D3 at 5,520 IU/kg, and 3) vitamin D3 at 2,760 IU/kg plus 25OHD at 2,760 IU (69 µg)/kg. The level of 25OHD in the serum was tested throughout the whole experimental period (0-95 wk). Bone growth rate (BGR) was measured at 10 wk using a calcein injection technique. Femurs were scanned using Micro-CT for 3D structural analysis, and the whole-body composition analysis was performed using a dual-energy x-ray absorptiometry method at 17, 60, and 95 wk. Dietary supplementation of 25OHD significantly increased 25OHD level in the serum from 0 to 95 wk. During the rearing period (0-17 wk), 25OHD increased BGR, cortical tissue volume, and bone marrow area at 17 wk, simultaneously. 25OHD created more pores in cortical bone, which resulted in a lower cortical bone mineral density (BMD) but without alerting bone mineral content (BMC). This effect allowed more space for mineral deposition in bones during the later egg-laying period. At 60 wk, the 25OHD group had significantly greater BMD, which led to the highest total BMC, cortical volume, and trabecular bone connectivity. At 95 wk, the birds fed 25OHD had significantly higher cortical bone volume and lower porosity. The 25OHD group also had higher total BMD and medullary bone volume but a lower BMC and volume of trabecular bone than vitamin D3 or double dosage vitamin D3 treatment. This indicated that the bone resorption rate was lower in cortical bone than that in trabecular bone in the late laying period. In conclusion, supplementation with dietary 25OHD could stimulate bone growth and increase bone volume in pullets to provide more space for mineral deposition during the laying period with positive effects on laying hen bone quality.

Effects of dietary Original XPC on selected blood variables in layer pullets challenged with Mycoplasma gallisepticum,.

Effects of dietary Original XPC (XPC) on 17 selected blood variables in commercial layer pullets challenged with the virulent, low-passage R strain of Mycoplasma gallisepticum (RlowMG) were investigated. Hy-Line W-36 pullets sourced from M. gallisepticum-clean layer breeders were fed a basal diet with XPC (1.25 kg/metric ton) or without from hatch until 12 wk of age (woa). At 8 and 10 woa, half of the birds in each dietary treatment were challenged with RlowMG. Blood samples were taken immediately before the initial RlowMG challenge at 8 woa and again at 12 woa (4 wk after challenge). At 8 woa, blood pH was lower and glucose concentration was higher in the preassigned challenge treatment groups. At 12 woa, the concentration of oxygen dissolved in the blood was significantly lower in the RlowMG-challenged group than the unchallenged group of birds regardless of dietary treatment. The RlowMG challenge significantly increased blood carbon dioxide partial pressure, calcium, sodium, anion gap, osmolality, glucose, and corticosterone levels but significantly decreased blood oxygen partial pressure, oxyhemoglobin concentration, concentration of oxygen dissolved in the blood, chloride, and pH levels. Because blood pH and glucose concentration at 8 woa were examined before challenge, their baseline values were biased with respect to challenge treatment before treatment was applied. However, the lack of a significant main effect due to diet at 8 woa for blood pH and glucose concentration, along with the other 15 blood variables, indicate that the baseline data with respect to dietary treatment were unbiased, allowing for real dietary effects to be accurately assessed. In conclusion, layer pullets challenged with RlowMG undergo a stress response associated with changes in various physiological blood variables, and a decrease in pH and increase in carbon dioxide partial pressure, in association with a lack of change in bicarbonate, indicates that the stress response caused by the RlowMG challenge was associated with respiratory acidosis. Nevertheless, feeding XPC did not influence the effects of challenge treatment on these postchallenge physiological blood values.

Supplementation of a water-soluble zinc-AA complex to reduce stress in broilers, pullets, and layers1.

This study consisted of three 5-wk experiments to test the effects of administering Zinpro-LQ (Zinpro-LQ, Zinpro Corporation, Eden Prairie, MN) for different time intervals in stressed broilers, pullets, and layers. Treatments included: nonstressed control (NC), stressed control (SC), stressed and supplemented Zinpro-LQ for 14 d prior to stressor (S1), 10 d prior (S2), 7 d prior to and 7 d during stressor (S3), and 5 d prior (S4). Birds included 1-d-old Cobb 500 male broilers (Experiment 1), 7-wk-old white Leghorn pullets (Experiment 2), and 50-wk-old white Leghorn layers (Experiment 3). All stressed birds were vaccinated against Newcastle Disease Virus (NDV) on day 28 and exposed to random feed and water withdrawal days 28 to 35 for 6 h/d. Pullets were beak trimmed on day 21, and layers were heat stressed days 28 to 35. Blood was sampled on day 35 to determine plasma chemistry, corticosterone (CORT), anti-NDV antibody titer, and heterophil/lymphocyte (H/L) ratio. Mortality, feed and water consumption, and BW were also recorded. In Experiment 1, CORT and H/L ratio were highest in SC (P < 0.05). In Experiment 2, CORT was higher in SC than S4 (P < 0.05) while all other treatments were statistically intermediate. NDV titer was lower in SC than S4 while all other treatments were statistically intermediate. Treatment differences were also observed for H/L ratio, and plasma uric acid and cholesterol in Experiment 2. In Experiment 3, treatment differences were observed for H/L ratio, plasma glucose, sodium, and chloride. H/L ratio was lower in NC, S1, and S4 compared with SC (P < 0.05); and NC and S1 were lower than S2 (P < 0.05). In conclusion, dietary inclusion of zinc AA complex for any length of time prior to a stressor helped reduce measures of stress in broilers. The S1 and S4 treatments helped reduce stress and improve humoral immune response in pullets and layers.

The effects of coarse and wet feeding on performance parameters, gastrointestinal tract and tibia traits, and digesta phytase activity in egg-type pullets, either fed a low or moderate phosphorus diet.

In a 2 × 2 × 2 factorial design, the effects of dietary non-phytate phosphorus (NPP) levels, 0.17% (low) and 0.33% (moderate), diet moisture (dry and wet), and diet particle size (coarse and fine), were studied on egg production, characteristics of gastrointestinal tract (GIT) and tibia, digesta pH, and phytase activity in layer pullets (16 to 28 wk of age). The low NPP diet increased average daily water intake (ADWI) to ADFI ratio (4.2%) from 16 to 17 wk, but decreased this ratio (2.8%) from 23 to 27 wk. It decreased ADFI (1.5%) and egg mass production (3.8%) from 19 to 22 wk. It decreased egg weight (0.29 g) and ADWI (2.1%) from 23 to 27 wk. At 22 wk, the GIT relative empty organ weights were (g/kg BW) higher for proventriculus + gizzard (0.96), duodeneum (0.94), and jejunum + ileum (1.95) with the low vs. moderate NPP diet. The low NPP diet decreased digesta phytase activity in crop and proventriculus+gizzard at 28 wk. The wet diet increased ADFI, ADWI, and ADWI/ADFI ratio from 16 to 27 wk, egg mass production (3.0%) from 19 to 22 wk, and egg weight (0.45 g) from 23 to 27 wk. The wet diet also increased digesta phytase activity in proventriculus+gizzard. The coarse diet decreased ADFI from 19 to 22 wk (1.7%) and 23 to 27 wk (1.2%). The coarse diet caused reduced egg mass production (2.6%) from 23 to 27 wk. Egg shell breaking strength was increased on the coarse diet (0.9 Newton). The coarse diet increased ADWI/ADFI ratio from 16 to 27 wk, and increased relative gizzard weight by 1.95 and 0.81 g/kg BW at 22 and 28 wk, respectively. The coarse diet increased jejunal/ileal pH with 0.16 units at 28 wk. None of the tested parameters affected tibia characteristics. It was concluded that a low NPP diet did not clearly affect the studied parameters. The wet diet increased ADFI, ADWI, and egg production. The coarse diet increased ADWI, egg shell breaking strength, relative gizzard weight, and reduced ADFI and egg production.

Influence of beak trimming and inclusion of sodium butyrate in the diet on growth performance and digestive tract traits of brown-egg pullets differing in initial body weight.

We studied the effects of beak trimming and sodium-butyrate inclusion in the diet on growth performance and gastrointestinal tract (GIT) traits of brown-egg pullets differing in initial BW. In experiment 1, a total of 6 treatments were organized as a 2 × 3 factorial with 2 BW at hatch (light, 33.9 g and heavy, 37.6 g) and 3 beak trimming protocols [mild (MI-0) or aggressive (AG-0) infrared power setting at hatch and traditional hot blade at 8 D of age (HB-8)] as main effects. Initial BW did not affect growth performance or GIT traits at any age. From hatch to 5 wk of age, HB-8 pullets had lower ADFI (P < 0.01) and ADG (P < 0.05) than MI-0 and AG-0 pullets but no differences were detected after this age. Beak trimming did not affect FCR, BW uniformity, GIT traits, or bacteria count in the excreta at any age. In experiment 2, a total of 12 treatments were organized as a 2 × 3 × 2 factorial, with 2 BW at hatch, 3 beak trimming protocol (as per in experiment 1), and 2 levels of a sodium-butyrate additive (0 vs. 0.3%) as main effects. At 7 D of life, beak treatment reduced pullet growth and AG-0 procedure impaired pullet uniformity (P < 0.001) but the birds recovered completely by day 14 (P < 0.001 for the interaction with time). Cumulatively (0 to 6 wk of age), pullets beak treated at hatch (MI-0 and AG-0) had greater ADFI than HB-8 pullets (P < 0.01). Sodium butyrate tended to improve ADG (P = 0.073) and FCR (P = 0.069) with most of the benefits observed for the first 2 wk of life. In summary, initial BW and beak trimming procedure did not affect final pullet growth in any of the 2 experiments, or GIT traits in experiment 1. Sodium butyrate tended to improve growth and FCR from 0 to 6 wk of age but did not affect BW uniformity.

Growth performance, apparent retention of components, and excreta dry matter content in Shaver White pullets (5 to 16 week of age) in response to dietary supplementation of graded levels of a single strain Bacillus subtilis probiotic.

Administered in adequate amounts, probiotics can be an alternative to antibiotic growth promoters in poultry production. This study evaluated dose response of a single strain of Bacillus subtilis (SSB, DSM29784) on growth performance, apparent retention (AR) of components, and excreta DM content in pullets. A basal corn-soybean meal diet was formulated to meet the specifications for grower (week 5 to 10) and developer (week 11 to 16) phases. In each phase, SSB was added to the basal diet to create 4 test diets: 0 (control, CON), 1.1E+08 (low; LSSB), 2.2E+08 (medium; MSSB), or 1.1E+09 (high; HSSB) CFU of SSB/kg of feed. All diets had TiO2 (0.5%) as a digestibility marker. A total of 720 day-old Shaver White chicks were placed in 48 cages (15 pullets per cage) and reared on a commercial antibiotic free diet for a 4-wk period. At the beginning of week 5, treatments were allocated based on cage BW (n = 12). Birds had free access to feed and water throughout. The BW, BW uniformity, feed intake (FI) and FCR were obtained weekly. Excreta was collected at the end of each phase for AR of DM, organic matter, CP, neutral detergent fiber, minerals, and AME, as well as excreta DM content. In response to SSB inclusion, BW improved in a linear (P < 0.005) and quadratic (P < 0.0001) manner in grower. FI decreased in a linear and quadratic pattern (P < 0.05, week 8 to 10) in grower, and linearly (P < 0.05) across the developer phase. Overall FCR improved in linear and quadratic pattern (P < 0.01) in the grower phase. In both the phases, AME improved in a quadratic pattern (P < 0.05). Inclusion of SSB had a linear reduction (P < 0.0001) in excreta moisture content in the grower phase. In summary, these results demonstrate that B. subtilis probiotic improved performance through enhanced nutrient utilization and reduced excreta moisture content indicating improved pullet gut health.

Assessment of the minimal available phosphorus needs of pullets during the pre-laying period.

Dietary phosphorus (P) oversupply is costly to the poultry industry and represents a point source of excessive P discharge to the environment via manure application to soils. The current study was conducted to investigate the effects of dietary available P (AP) level on growth performance, plasma biochemistry, bone characteristics, and P flow in order to assess minimal AP needs of pullets during the pre-lay period. A total of 192 Lohmann pullet chicks were randomly allocated to one of 4 treatments with 8 replicate cages of 6 birds per cage. The pullets of Low (L; the lowest AP regimen) were fed diets in a sequence of 0.200, 0.175, and 0.150% AP for the age period of 0 to 4 wk, 4 to 8 wk, and 8 to 16 wk, respectively. The AP level was increased by an increment of 0.1% in each phase in Low-Medium (LM), Medium-High (MH) and High (H), thus making H (control) consisting of 0.500, 0.475, and 0.450% AP. Overall, there were no main effects of treatment on body weight gain (BWG), feed intake (FI) and feed conversion ratio (FCR). However, compared with the H treatment, BWG or FI of the L treatment was lower during the first 8 wk post-hatch, but became comparable or higher over the last 8 wk (P < 0.05); FCR of L birds was greater over wk 0 to 4 but became smaller over wk 8 to 16 (P < 0.05). Plasma P was lower in L than H treatments at wk 8 but turned comparable at wk 16 (P < 0.001). Bone characteristics were not different for pullets fed various AP regimens. Total P intake and excretion were reduced with lowering dietary AP (P < 0.001), but its retention percent was not affected. The results indicated that the lowest AP regimen (0.200-0.175-0.150%) in the current study was adequate to support healthy growth and development of pre-lay pullets, while reducing P excretion in manure.

Supplementing the feeds of layer pullets, at different ages with two different fiber sources improves immune function.

Two experiments were conducted to study the effects of lignocellulose supplementation on immune function in layer pullets at different stages of growth. Four-wk-old pullets (Experiment 1) were fed a control, diet (Diet C); Diet C plus 1% mixed soluble/insoluble fiber (Diet MF), or plus 1% insoluble fiber (Diet IF). At 7.5 wk-of-age, heterophil phagocytosis, and oxidative burst in Groups MF (328.5 beads/100 cells; 4,330.0 ΔRFU; relative fluorescent units) and IF (350.3; 5,264.4) were greater (P < 0.05) than Controls (303.4; 3,509.0). At 8 wk-of-age, Group MF and IF relative weights of bursa of Fabricius (0.57 g/100 g BW; 0.58 g /100 g BW), thymus glands (0.77; 0.78), and areas of Peyer's patches (PP) (2.7 cm2; 2.9 cm2) were higher (P < 0.05) than Controls (bursa, 0.50 g; thymus, 0.70 g; PP area, 1.8 cm2). In Experiment 2, 10-wk-old pullets were fed a control diet or diets containing 1.5% MF or IF for 8 wk. At 14 wk-of-age IF pullets had higher (P < 0.05) heterophil phagocytosis efficiency (447.9 beads/100 cells) than Controls (376.4) and MF and IF had greater (P < 0.05) oxidative burst (1,302.9 and 1,857.7 ΔRFU) than Controls (744.1). At 17 wk-of-age MF and IF had increased (P < 0.05) proliferation of T-lymphocytes (ConcanavalinA-stimulated) (100.4 and 103.1% of unstimulated cells) and B-lymphocytes (lipopolysaccharide-stimulated) (122.4 and 129.0) than Controls (ConA, 79.4; lipopolysaccharide, 106.6). At 18 wk-of-age, IF pullets were heavier (1,607.5 g, P < 0.05) than Controls (1,506.5 g), had heavier (P < 0.05) bursa of Fabricius (1.12 g) than MF and Control groups (0.98 g; 0.92 g) and cecal tonsils of MF (0.38 g) and IF (0.39 g) weighted more (P < 0.05) than Controls (0.33 g). Number of jejunal and ileal PP (10.0) in IF pullets was higher (P < 0.05) than Controls (7.1). These results indicate that both MF and IF can improve development of the immune system of young and grower pullets during periods of maturation and involution of lymphoid organs.

Dietary supplementation with the clay mineral palygorskite affects performance and beneficially modulates caecal microbiota in laying pullets.

AIMS: The objective of this study was twofold: (i) to examine the effect of early-posthatch dietary inclusion of the clay mineral palygorskite on laying pullets' growth and (ii) to determine the potential effects of the latter diet on caecal microbial communities. METHODS AND RESULTS: Four hundred and thirty-two female, 1-day-old Hyline Brown chickens were equally and randomly allocated to two dietary treatment groups; control and palygorskite (0 and 0·5% palygorskite respectively). They were reared in floor pens for 18 weeks. Feed intake, feed conversion ratio, body weight gain and uniformity were assessed. At the end of the rearing period, 53 birds per group were transferred to laying cages without any changes in their respective dietary treatments. At onset of lay, eight birds from each group were euthanized and their caeca were obtained for analysis. Culture-independent and sequencing-based methods were used to analyse caecal microbial composition. CONCLUSIONS: Palygorskite dietary supplementation increased the uniformity of the relative group at the end of the rearing period. Pullets that received palygorskite had different and more homogeneous caecal microbial profiles at onset of lay when compared to controls. Moreover, in the caeca of the former pullets a more favourable microbiota was evident; it comprised largely of bacteria that are major degraders of resistant polysaccharides and efficient in butyrate production. SIGNIFICANCE AND IMPACT OF THE STUDY: The novelty of this study lies to the beneficial effect of palygorskite (a clay mineral) to pullets growth and health, likely through modulation of the gut microbiota.

Metabolic and clinical response to Escherichia coli lipopolysaccharide in layer pullets of different genetic backgrounds supplied with graded dietary L-arginine.

L-arginine (Arg) is an essential amino acid in birds that plays a decisive role in avian protein synthesis and immune response. Effects of graded dietary Arg supply on metabolic and clinical response to Escherichia coli lipopolysaccharide (LPS) were studied over 48 hours after a single intramuscular LPS injection in 18-week-old genetically diverse purebred pullets. LPS induced a genotype-specific fever response within 4 hours post injectionem. Whereas brown genotypes showed an initial hypothermia followed by longer-lasting moderate hyperthermia, white genotypes exhibited a biphasic hyperthermia without initial hypothermia. Furthermore, within 2 hours after LPS injection, sickness behavior characterized by lethargy, anorexia, intensified respiration, and ruffled feathers appeared, persisted for 3 to 5 hours and recovered 12 hours post injectionem. The varying grades of Arg did not alter the examined traits named above, whereas insufficient Arg reduced body growth and increased relative weights of liver and pancreas significantly. At 48 hours post injectionem, increased relative weights of liver and spleen were also found in LPS treated pullets, whereas LPS decreased those of pancreas, bursa, thymus, and cecal tonsils. Moreover, LPS lowered the sum of plasma amino acids and decreased plasma concentrations of Arg, citrulline, glutamate, methionine, ornithine, phenylalanine, proline, tryptophan, and tyrosine, and increased those of aspartate, glutamine, lysine, 1- and 3-methyl-histidine. Elevating concentrations of dietary Arg led to increasing plasma concentrations of Arg, citrulline, ornithine, and 3-methyl-histidine subsequently. As quantitative expression of LPS-induced anorexia, proteolysis, and the following changes in plasma amino acids, pullets showed a significant decrease of feed and nitrogen intake and catabolic metabolism characterized by negative nitrogen balance and body weight loss in the first 24 hours post injectionem. Pullets recovered from the challenge within the second 24 hours post injectionem and changed to anabolism with re-increased feed and nitrogen intake, positive nitrogen retention, and weight gain. To conclude, present results confirmed that LPS induced numerous metabolic and physiological changes in pullet's genotypes, whereas dietary Arg affected the examined traits only slightly.

Effect of insoluble fiber supplementation applied at different ages on digestive organ weight and digestive enzymes of layer-strain poultry.

Two experiments were conducted to study effects of dietary insoluble fiber (IF) on digestive enzyme function in layer poultry. In Experiment 1, 8 wk old pullets were fed a control diet (Group C) or a diet (Group IF) supplemented with 1% IF (Arbocel RC). After 5 wk, 6 pullets per group were killed and organ samples collected. The remaining pullets in Group C were divided into two groups: half were fed the control diet (Group C) and half were given the IF diet (Group C-IF). Similarly, half the pullets in Group IF continued on the IF diet (Group IF) and half on the control diet (Group IF-C). At 10 wk, organ samples were collected. BW at wk 5 (IF, 1364.8 g; C, 1342.9 g) and 10 wk (IF, 1678.1 g; IF-C, 1630.5 g; C-IF, 1617.1 g; C, 1580.4 g) were not different. At wk 5, the relative proventricular weight (0.41 g/100 g BW) and activities of pepsin (75.3 pepsin units/g proventriculus/min) and pancreatic general proteolytic activity (GP) (122.9 µmol tyrosine produced/g tissue) were greater (P < 0.05) than those of Group C (proventricular relative weight, 0.36; pepsin activity, 70.6; GP activity, 94.3). At wk 10, relative weights of liver and gizzard of Group IF were heavier (P < 0.05) than other treatments; activities of pepsin, GP, trypsin and chymotrypsin of IF pullets were significantly greater than other treatments as was mRNA expression for pepsinogens A (25.9 vs. 22.9) and C (13.1 vs. 10.8). In Experiment 2, 19 wk old hens were fed a control diet or a diet containing 0.8% IF (Arbocel RC) for 12 wk. Final BW after 12 wk was not different (IF, 1919.4 g; C, 1902.1 g). Pancreatic GP activity was greater (P < 0.05) in Group IF hens than Group C at wk 12 (122.2 vs. 97.0 µmol tyrosine released/min/g tissue)) as was relative gizzard weight (1.32 vs 1.10 g/100 g BW). The significantly improved digestive organ weights and enzyme activities in IF pullets may contribute to an improvement in feed utilization.