Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 44
Filter
Add more filters

Complementary Medicines
Country/Region as subject
Publication year range
1.
Toxins (Basel) ; 13(5)2021 05 07.
Article in English | MEDLINE | ID: mdl-34067049

ABSTRACT

Prostate cancer is one of the most common cancers in men. Despite the development of a variety of therapeutic agents to treat either metastatic hormone-sensitive prostate cancer, advanced prostate cancer, or nonmetastatic/metastatic castration-resistant prostate cancer, the progression or spread of the disease often cannot be avoided. Additionally, the development of resistance of prostate cancer cells to available therapeutic agents is a well-known problem. Despite extensive and cost-intensive research over decades, curative therapy for metastatic prostate cancer is still not available. Therefore, additional therapeutic agents are still needed. The animal kingdom offers a valuable source of natural substances used for the treatment of a variety of diseases. Bee venom of the honeybee is a mixture of many components. It contains proteins acting as enzymes such as phospholipase A2, smaller proteins and peptides such as melittin and apamin, phospholipids, and physiologically active amines such as histamine, dopamine, and noradrenaline. Melittin has been shown to induce apoptosis in different cancer cell lines, including prostate cancer cell lines. It also influences cell proliferation, angiogenesis, and necrosis as well as motility, migration, metastasis, and invasion of tumour cells. Hence, it represents an interesting anticancer agent. In this review article, studies about the effect of bee venom components on prostate cancer cells are discussed. An electronic literature research was performed utilising PubMed in February 2021. All scientific publications, which examine this interesting subject, are discussed. Furthermore, the different types of application of these promising substances are outlined. The studies clearly indicate that bee venom or melittin exhibited anticancer effects in various prostate cancer cell lines and in xenografts. In most of the studies, a combination of bee venom or the modified melittin with another molecule was utilised in order to avoid side effects and, additionally, to target selectively the prostate cancer cells or the surrounding tissue. The studies showed that systemic side effects and unwanted damage to healthy tissue and organs could be minimised when the anticancer drug was not activated until binding to the cancer cells or the surrounding tissue. Different targets were used, such as the matrix metalloproteinase 2, hormone receptors expressed by prostate cancer cells, the extracellular domain of PSMA, and the fibroblast activation protein occurring in the stroma of prostate cancer cells. Another approach used loaded phosphate micelles, which were cleaved by the enzyme secretory phospholipase A2 produced by prostate cancer cells. In a totally different approach, targeted nanoparticles containing the melittin gene were used for prostate cancer gene therapy. By the targeted nonviral gene delivery, the gene encoding melittin was delivered to the prostate cancer cells without systemic side effects. This review of the scientific literature reveals totally different approaches using bee venom, melittin, modified melittin, or protoxin as anticancer agents. The toxic agents acted through several different mechanisms to produce their anti-prostate cancer effects. These mechanisms are not fully understood yet and more experimental studies are necessary to reveal the complete mode of action. Nevertheless, the researchers have conducted pioneering work. Based on these results, further experimental and clinical studies about melittin and modifications of this interesting agent deriving from nature are necessary and could possibly lead to a complementary treatment option for prostate cancer.


Subject(s)
Antineoplastic Agents/pharmacology , Bee Venoms/pharmacology , Prostatic Neoplasms/drug therapy , Animals , Antineoplastic Agents/administration & dosage , Antineoplastic Agents/chemistry , Apamin/isolation & purification , Apamin/pharmacology , Apoptosis/drug effects , Bee Venoms/administration & dosage , Bee Venoms/chemistry , Bees , Humans , Male , Melitten/isolation & purification , Melitten/pharmacology , Phospholipases A2/isolation & purification , Phospholipases A2/pharmacology , Prostatic Neoplasms/pathology
3.
Synapse ; 75(2): e22185, 2020 02.
Article in English | MEDLINE | ID: mdl-32779216

ABSTRACT

Aging is a complex process that can lead to neurodegeneration and, consequently, several pathologies, including dementia. Physiological aging leads to changes in several body organs, including those of the central nervous system (CNS). Morphological changes in the CNS and particularly the brain result in motor and cognitive deficits affecting learning and memory and the circadian cycle. Characterizing neural modifications is critical to designing new therapies to target aging and associated pathologies. In this review, we compared aging to the changes occurring within the brain and particularly the limbic system. Then, we focused on key natural compounds, apamin, cerebrolysin, Curcuma longa, resveratrol, and N-PEP-12, which have shown neurotrophic effects particularly in the limbic system. Finally, we drew our conclusions delineating future perspectives for the development of novel natural therapeutics to ameliorate aging-related processes.


Subject(s)
Aging/drug effects , Limbic System/drug effects , Neurons/drug effects , Neuroprotective Agents/pharmacology , Nootropic Agents/pharmacology , Aging/metabolism , Amino Acids/pharmacology , Animals , Apamin/pharmacology , Curcuma , Limbic System/metabolism , Neuronal Plasticity/drug effects , Neurons/metabolism , Plant Extracts/pharmacology , Rats , Resveratrol/pharmacology
4.
Molecules ; 25(14)2020 Jul 10.
Article in English | MEDLINE | ID: mdl-32664327

ABSTRACT

Phellinus linteus is a well-known medicinal mushroom that is widely used in Asian countries. In several experimental models, Phellinus linteus extracts were reported to have various biological effects, including anti-inflammatory, anti-cancer, hepatoprotective, anti-diabetic, neuroprotective, and anti-angiogenic activity. In the present study, several bioactive compounds, including palmitic acid ethyl ester and linoleic acid, were identified in Phellinus linteus. The intermediate-conductance calcium-activated potassium channel (IKCa) plays an important role in the regulation of the vascular smooth muscle cells' (VSMCs) contraction and relaxation. The activation of the IKCa channel causes the hyperpolarization and relaxation of VSMCs. To examine whether Phellinus linteus extract causes vasodilation in the mesenteric arteries of rats, we measured the isometric tension using a wire myograph. After the arteries were pre-contracted with U46619 (a thromboxane analogue, 1 µM), Phellinus linteus extract was administered. The Phellinus linteus extract induced vasodilation in a dose-dependent manner, which was independent of the endothelium. To further investigate the mechanism, we used the non-selective K+ channel blocker tetraethylammonium (TEA). TEA significantly abolished Phellinus linteus extract-induced vasodilation. Thus, we tested three different types of K+ channel blockers: iberiotoxin (BKca channel blocker), apamin (SKca channel blocker), and charybdotoxin (IKca channel blocker). Charybdotoxin significantly inhibited Phellinus linteus extract-induced relaxation, while there was no effect from apamin and iberiotoxin. Membrane potential was measured using the voltage-sensitive dye bis-(1,3-dibutylbarbituric acid)-trimethine oxonol (DiBAC4(3)) in the primary isolated vascular smooth muscle cells (VSMCs). We found that the Phellinus linteus extract induced hyperpolarization of VSMCs, which is associated with a reduced phosphorylation level of 20 KDa myosin light chain (MLC20).


Subject(s)
Basidiomycota/chemistry , Mesenteric Arteries/drug effects , Plant Extracts/pharmacology , Vasodilation/drug effects , 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid/pharmacology , Animals , Apamin/pharmacology , Charybdotoxin/pharmacology , Endothelium, Vascular/drug effects , Endothelium, Vascular/metabolism , Male , Membrane Potentials/drug effects , Mesenteric Arteries/metabolism , Muscle Contraction/drug effects , Muscle, Smooth, Vascular/drug effects , Muscle, Smooth, Vascular/metabolism , Myocytes, Smooth Muscle/drug effects , Myocytes, Smooth Muscle/metabolism , Myosin Light Chains/metabolism , Peptides/pharmacology , Phellinus , Phosphorylation/drug effects , Potassium Channel Blockers/pharmacology , Rats , Rats, Sprague-Dawley , Tetraethylammonium/pharmacology , Vasoconstriction/drug effects
5.
J Ethnopharmacol ; 257: 112860, 2020 Jul 15.
Article in English | MEDLINE | ID: mdl-32289477

ABSTRACT

ETHNOPHARMACOLOGICAL RELEVANCE: Bee venom (BV) has been used for the treatment of inflammatory diseases, such as rheumatoid arthritis, and for the relief of pain in traditional oriental medicine. AIM OF STUDY: The aim of this study was to determine the anti-inflammatory effect of BV on monosodium urate (MSU)-induced gouty arthritis in a mouse model. MATERIALS AND METHODS: To develop a mouse model of acute gouty arthritis, 4 mg 50 µL-1 of MSU crystal suspension was injected intradermally into the right paw. After MSU crystal injection, we evaluated inflammatory cytokine production in mice of the BV-treated (0.5 and 1 mg kg-1 body weight) and apamin (APM)-treated (0.5 and 1 mg kg-1 body weight) groups. The positive control group was administered a colchicine (1 mg kg-1 body weight) injection with MSU crystals. RESULTS: BV and APM treatment suppressed inflammatory paw edema in MSU-administered mice. It also exerted anti-inflammatory effects in mice with gouty arthritis by inhibiting proinflammatory cytokine production and inflammasome formation. Interestingly, MSU crystal formation was decreased by BV and APM treatment. CONCLUSIONS: These results suggest that the APM from BV might be useful for the treatment of gouty arthritis due to its anti-inflammatory activities.


Subject(s)
Anti-Inflammatory Agents/pharmacology , Apamin/pharmacology , Arthritis, Experimental/drug therapy , Arthritis, Gouty/prevention & control , Bee Venoms/pharmacology , Joints/drug effects , Animals , Apamin/isolation & purification , Arthritis, Experimental/chemically induced , Arthritis, Experimental/metabolism , Arthritis, Experimental/pathology , Arthritis, Gouty/chemically induced , Arthritis, Gouty/metabolism , Arthritis, Gouty/pathology , Bee Venoms/chemistry , Cytokines/genetics , Cytokines/metabolism , Inflammasomes/genetics , Inflammasomes/metabolism , Inflammation Mediators/metabolism , Joints/metabolism , Joints/pathology , Macrophages/drug effects , Macrophages/metabolism , Male , Mice , Mice, Inbred C57BL , RAW 264.7 Cells , Signal Transduction , Uric Acid
6.
J Ethnopharmacol ; 229: 22-28, 2019 Jan 30.
Article in English | MEDLINE | ID: mdl-30287194

ABSTRACT

ETHNOPHARMACOLOGICAL RELEVANCE: A major concern in modern society involves the lasting detrimental behavioral effects of exposure to alcoholic beverages. Consequently, hundreds of folk remedies for hangover have been suggested, most of them without a scientific basis, for lack of proper test systems. Over centuries, yellow toadflax (Linaria vulgaris Mill., Lv) tincture has been used in Russian traditional medicine to treat the spectrum of hangover symptoms such as vertigo, headache, drunken behaviors, and as a sedative. MATERIALS AND METHODS: Here we use in-vitro cultured hippocampal neurons to examine the effect of the Lv extract as well as the flavonoid acetylpectolinarin (ACP) exclusively found in Lv extract, on spontaneous network activity of the cultured neurons exposed to low, physiological concentrations of ethanol. RESULTS: As in previous studies, low (0.25-0.5%) ethanol causes an increase in network activity, which was converted to suppression, with high concentrations of ethanol. Lv extract and ACP, at low concentrations, had no appreciable effect on spontaneous activity, but they blocked the facilitating action of low ethanol. This action of ACP was also seen when the culture was exposed to 1-EBIO, a SK potassium channel opener, and was blocked by apamin, an SK channel antagonist. In contrast, ACP or Lv extracts did not reverse the suppressive effects of higher ethanol. CONCLUSIONS: Our results suggest that ACP acts by interacting with the SK channel, to block the facilitatory effect of low concentration of ethanol, on network activity in hippocampal cultures.


Subject(s)
Chromones/pharmacology , Ethanol/adverse effects , Flavonoids/pharmacology , Hippocampus/drug effects , Linaria , Plant Extracts/pharmacology , Apamin/pharmacology , Hippocampus/physiology , Medicine, Traditional , Potassium Channel Blockers/pharmacology , Russia
7.
J Physiol ; 595(4): 1239-1251, 2017 02 15.
Article in English | MEDLINE | ID: mdl-27767209

ABSTRACT

KEY POINTS: We establish experimental preparations for optogenetic investigation of glutamatergic input to the inferior olive. Neurones in the principal olivary nucleus receive monosynaptic extra-somatic glutamatergic input from the neocortex. Glutamatergic inputs to neurones in the inferior olive generate bidirectional postsynaptic potentials (PSPs), with a fast excitatory component followed by a slower inhibitory component. Small conductance calcium-activated potassium (SK) channels are required for the slow inhibitory component of glutamatergic PSPs and oppose temporal summation of inputs at intervals ≤ 20 ms. Active integration of synaptic input within the inferior olive may play a central role in control of olivo-cerebellar climbing fibre signals. ABSTRACT: The inferior olive plays a critical role in motor coordination and learning by integrating diverse afferent signals to generate climbing fibre inputs to the cerebellar cortex. While it is well established that climbing fibre signals are important for motor coordination, the mechanisms by which neurones in the inferior olive integrate synaptic inputs and the roles of particular ion channels are unclear. Here, we test the hypothesis that neurones in the inferior olive actively integrate glutamatergic synaptic inputs. We demonstrate that optogenetically activated long-range synaptic inputs to the inferior olive, including projections from the motor cortex, generate rapid excitatory potentials followed by slower inhibitory potentials. Synaptic projections from the motor cortex preferentially target the principal olivary nucleus. We show that inhibitory and excitatory components of the bidirectional synaptic potentials are dependent upon AMPA (GluA) receptors, are GABAA independent, and originate from the same presynaptic axons. Consistent with models that predict active integration of synaptic inputs by inferior olive neurones, we find that the inhibitory component is reduced by blocking large conductance calcium-activated potassium channels with iberiotoxin, and is abolished by blocking small conductance calcium-activated potassium channels with apamin. Summation of excitatory components of synaptic responses to inputs at intervals ≤ 20 ms is increased by apamin, suggesting a role for the inhibitory component of glutamatergic responses in temporal integration. Our results indicate that neurones in the inferior olive implement novel rules for synaptic integration and suggest new principles for the contribution of inferior olive neurones to coordinated motor behaviours.


Subject(s)
Olivary Nucleus/metabolism , Receptors, AMPA/metabolism , Synaptic Potentials , Animals , Apamin/pharmacology , Glutamic Acid/metabolism , Large-Conductance Calcium-Activated Potassium Channels/metabolism , Mice , Mice, Inbred C57BL , Motor Cortex/cytology , Motor Cortex/metabolism , Motor Cortex/physiology , Neurons/metabolism , Neurons/physiology , Olivary Nucleus/cytology , Olivary Nucleus/physiology , Peptides/pharmacology , Potassium Channel Blockers/pharmacology , Small-Conductance Calcium-Activated Potassium Channels/metabolism , Synapses/metabolism , Synapses/physiology
8.
Atherosclerosis ; 250: 15-22, 2016 07.
Article in English | MEDLINE | ID: mdl-27175607

ABSTRACT

BACKGROUND AND AIMS: Small mesenteric artery resistance and functionality are key factors for the maintenance of blood homeostasis. We attained to evaluate the effects of a rice bran enzymatic extract (RBEE) on structural, mechanic and myogenic alterations and endothelial dysfunction secondary to atherosclerosis disease. METHODS: Seven week-old ApoE(-/-) mice were fed on standard (ST) or high fat (HF) diets supplemented or not with 1 or 5% RBEE (w/w) for 23 weeks. Wild-type C57BL/6J mice fed on ST diet served as controls. Small mesenteric arteries were mounted in a pressure myograph in order to evaluate structural, mechanical and myogenic properties. Vascular reactivity was assessed in the presence of different combinations of inhibitors: l-NAME, indometacin, apamin and charybdotoxin. RESULTS: ApoE(-/-) mice fed on ST and HF diets showed different structural and mechanical alterations, alleviated by RBEE supplementation of ST and HF diets. C57BL/6J was characterized by increased expression of IKCa (199.3%, p = 0.023) and SKCa (133.2%, p = 0.026), resulting in higher EDHF participation (p = 0.0001). However, NO release was more relevant to ApoE(-/-) mice vasodilatation. HF diet reduced the amount of NO released due to 2-fold increase of eNOS phosphorylation in the inhibitory residue Thr495 (p = 0.034), which was fully counteracted by RBEE supplementation (p = 0.028), restoring ACh-induced vasodilatation (p = 0.00006). Dihydroethidium fluorescence of superoxide and picrosirius red staining of collagen were reduced by RBEE supplementation of HF diet by 76.91% (p = 0.022) and 65.87% (p = 0.030), respectively. CONCLUSION: RBEE supplemented diet reduced vessel remodeling and oxidative stress. Moreover, RBEE supplemented diet increased NO release by downregulating p-eNOS(Thr495), thus, protecting the endothelial function.


Subject(s)
Dietary Supplements , Endothelium, Vascular/metabolism , Oryza/chemistry , Vascular Remodeling , Animals , Apamin/pharmacology , Arteries/metabolism , Charybdotoxin/pharmacology , Collagen/chemistry , Elastin/chemistry , Indomethacin/pharmacology , Male , Mice , Mice, Inbred C57BL , Mice, Knockout, ApoE , Microcirculation , NG-Nitroarginine Methyl Ester/pharmacology , Nitric Oxide/chemistry , Nitric Oxide Synthase Type III/metabolism , Oxidative Stress , Oxygen/chemistry , Superoxides/chemistry , Vascular Stiffness , Vasodilation
9.
J Neurosci ; 35(26): 9701-6, 2015 Jul 01.
Article in English | MEDLINE | ID: mdl-26134652

ABSTRACT

Hearing loss among the elderly correlates with diminished social, mental, and physical health. Age-related cochlear cell death does occur, but growing anatomical evidence suggests that synaptic rearrangements on sensory hair cells also contribute to auditory functional decline. Here we present voltage-clamp recordings from inner hair cells of the C57BL/6J mouse model of age-related hearing loss, which reveal that cholinergic synaptic inputs re-emerge during aging. These efferents are functionally inhibitory, using the same ionic mechanisms as do efferent contacts present transiently before the developmental onset of hearing. The strength of efferent inhibition of inner hair cells increases with hearing threshold elevation. These data indicate that the aged cochlea regains features of the developing cochlea and that efferent inhibition of the primary receptors of the auditory system re-emerges with hearing impairment. SIGNIFICANCE STATEMENT: Synaptic changes in the auditory periphery are increasingly recognized as important factors in hearing loss. To date, anatomical work has described the loss of afferent contacts from cochlear hair cells. However, relatively little is known about the efferent innervation of the cochlea during hearing loss. We performed intracellular recordings from mouse inner hair cells across the lifespan and show that efferent innervation of inner hair cells arises in parallel with the loss of afferent contacts and elevated hearing threshold during aging. These efferent neurons inhibit inner hair cells, raising the possibility that they play a role in the progression of age-related hearing loss.


Subject(s)
Cochlea/pathology , Hair Cells, Auditory, Inner/physiology , Hearing Loss/pathology , Neural Inhibition/physiology , Acetylcholine/pharmacology , Age Factors , Alcohol Oxidoreductases , Animals , Animals, Newborn , Apamin/pharmacology , Calcium Channel Blockers/pharmacology , Co-Repressor Proteins , Conotoxins/pharmacology , Curare/pharmacology , DNA-Binding Proteins/metabolism , Disease Models, Animal , Evoked Potentials, Auditory, Brain Stem/drug effects , Evoked Potentials, Auditory, Brain Stem/physiology , Female , Glycine Agents/pharmacology , Hearing Loss/physiopathology , Mice , Mice, Inbred C57BL , Neuromuscular Nondepolarizing Agents/pharmacology , Phosphoproteins/metabolism , Strychnine/pharmacology
10.
Toxins (Basel) ; 7(4): 1126-50, 2015 Apr 01.
Article in English | MEDLINE | ID: mdl-25835385

ABSTRACT

While knowledge of the composition and mode of action of bee and wasp venoms dates back 50 years, the therapeutic value of these toxins remains relatively unexploded. The properties of these venoms are now being studied with the aim to design and develop new therapeutic drugs. Far from evaluating the extensive number of monographs, journals and books related to bee and wasp venoms and the therapeutic effect of these toxins in numerous diseases, the following review focuses on the three most characterized peptides, namely melittin, apamin, and mastoparan. Here, we update information related to these compounds from the perspective of applied science and discuss their potential therapeutic and biotechnological applications in biomedicine.


Subject(s)
Apamin , Melitten , Peptides , Wasp Venoms , Animals , Apamin/pharmacology , Apamin/therapeutic use , Humans , Intercellular Signaling Peptides and Proteins , Melitten/pharmacology , Melitten/therapeutic use , Peptides/pharmacology , Peptides/therapeutic use , Wasp Venoms/pharmacology , Wasp Venoms/therapeutic use
11.
Life Sci ; 115(1-2): 15-21, 2014 Oct 12.
Article in English | MEDLINE | ID: mdl-25242515

ABSTRACT

AIMS: Ginsenosides, active components in ginseng, have been shown to increase nitric oxide (NO) production in aortic endothelial cells. This effect was reversed by tetraethylammonium (TEA) inhibition of endothelial Ca(2+)-activated K(+) (KCa) channels. The objectives of this study, therefore, were to test 1) whether vasorelaxing ginsenoside Re could affect KCa current, an important regulator of NO production, in human coronary artery endothelial cells (HCAECs); and 2) whether small-conductance KCa (SKCa) channel was the channel subtype involved. MAIN METHODS: Ionic currents of cultured HCAECs were studied using whole-cell patch clamp technique. KEY FINDINGS: Ginsenoside Re dose-dependently increased endothelial outward currents, with an EC50 of 408.90±1.59nM, and a maximum increase of 36.20±5.62% (mean±SEM; p<0.05). Apamin, an SKCa channel inhibitor, could block this effect, while La(3+), a nonselective cation channel (NSC) blocker, could not. When NSC channel, inward-rectifier K(+) channel, intermediate-, and large-conductance KCa channels were simultaneously blocked, ginsenoside Re could still increase outward currents significantly (35.49±4.22%; p<0.05); this effect was again abolished by apamin. Repeating the experiments when Cl(-) channel was additionally blocked gave similar results. Finally, we demonstrated that ginsenoside Re could hyperpolarize HCAECs; this effect was reversed by apamin. These data clearly indicate that ginsenoside Re increased HCAEC outward current via SKCa channel activation, and NSC channel was not involved. SIGNIFICANCE: This is the first report to demonstrate that ginsenoside Re could increase SKCa channel activity in HCAECs. This can be a mechanism mediating ginseng's beneficial actions on coronary vessels.


Subject(s)
Endothelial Cells/drug effects , Endothelial Cells/metabolism , Ginsenosides/pharmacology , Small-Conductance Calcium-Activated Potassium Channels/metabolism , Vasodilator Agents/pharmacology , Apamin/pharmacology , Cell Line , Coronary Vessels/cytology , Humans , Lanthanum/pharmacology , Panax/chemistry , Patch-Clamp Techniques , Small-Conductance Calcium-Activated Potassium Channels/agonists , Small-Conductance Calcium-Activated Potassium Channels/antagonists & inhibitors
12.
Mol Pharm ; 11(9): 3210-22, 2014 Sep 02.
Article in English | MEDLINE | ID: mdl-25098949

ABSTRACT

Faced with the complex medical challenge presented by spinal cord injuries (SCI) and considering the lack of any available curative therapy, the development of a novel method of delivering existing drugs or candidate agents can be perceived to be as important as the development of new therapeutic molecules. By combining three ingredients currently in clinical use or undergoing testing, we have designed a central nervous system targeted delivery system based on apamin-modified polymeric micelles (APM). Apamin, one of the major components of honey bee venom, serves as the targeting moiety, poly(ethylene glycol) (PEG) distearoylphosphatidylethanolamine (DSPE) serves as the drug-loaded material, and curcumin is used as the therapeutic agent. Apamin was conjugated with NHS (N-hydroxysuccinimide)-PEG-DSPE in a site-specific manner, and APM were prepared by a thin-film hydration method. A formulation comprising 0.5 mol % targeting ligand with 50 nm particle size showed strong targeting efficiency in vivo and was evaluated in pharmacodynamic assays. A 7-day treatment by daily intravenous administration of low doses of APM (corresponding to 5 mg/kg of curcumin) was performed. Significantly enhanced recovery and prolonged survival was found in the SCI mouse model, as compared to sham-treated groups, with no apparent toxicity. A single dose of apamin-conjugated polymers was about 700-fold lower than the LD50 amount, suggesting that APM and apamin have potential for clinical applications as spinal cord targeting ligand for delivery of agents in treatment of diseases of the central nervous system.


Subject(s)
Apamin/pharmacology , Spinal Cord Injuries/drug therapy , Animals , Apamin/chemistry , Chemistry, Pharmaceutical/methods , Curcumin/chemistry , Drug Carriers/chemistry , Drug Delivery Systems/methods , Mice , Micelles , Particle Size , Phosphatidylethanolamines/chemistry , Polyethylene Glycols/chemistry , Polymers/chemistry , Succinimides/chemistry
13.
Arch Pharm Res ; 37(8): 1016-31, 2014 Aug.
Article in English | MEDLINE | ID: mdl-24293065

ABSTRACT

Diabetes is one of the leading causes of impaired wound healing. The objective of this study was to develop a bee venom-loaded wound dressing with an enhanced healing and anti-inflammatory effects to be examined in diabetic rats. Different preparations of polyvinyl alcohol (PVA), chitosan (Chit) hydrogel matrix-based wound dressing containing bee venom (BV) were developed using freeze-thawing method. The mechanical properties such as gel fraction, swelling ratio, tensile strength, percentage of elongation and surface pH were determined. The pharmacological activities including wound healing and anti-inflammatory effects in addition to primary skin irritation and microbial penetration tests were evaluated. Moreover, hydroxyproline, glutathione and IL-6 levels were measured in the wound tissues of diabetic rats. The bee venom-loaded wound dressing composed of 10 % PVA, 0.6 % Chit and 4 % BV was more swellable, flexible and elastic than other formulations. Pharmacologically, the bee venom-loaded wound dressing that has the same previous composition showed accelerated healing of wounds made in diabetic rats compared to the control. Moreover, this bee venom-loaded wound dressing exhibited anti-inflammatory effect that is comparable to that of diclofenac gel, the standard anti-inflammatory drug. Simultaneously, wound tissues covered with this preparation displayed higher hydroxyproline and glutathione levels and lower IL-6 levels compared to control. Thus, the bee venom-loaded hydrogel composed of 10 % PVA, 0.6 % Chit and 4 % BV is a promising wound dressing with excellent forming and enhanced wound healing as well as anti-inflammatory activities.


Subject(s)
Anti-Inflammatory Agents/therapeutic use , Apamin/therapeutic use , Chitosan/chemistry , Cross-Linking Reagents/chemistry , Diabetes Mellitus, Experimental/complications , Drug Carriers/chemistry , Polyvinyl Alcohol/chemistry , Wound Healing/drug effects , Alloxan/pharmacology , Animals , Anti-Inflammatory Agents/administration & dosage , Anti-Inflammatory Agents/adverse effects , Anti-Inflammatory Agents/pharmacology , Apamin/administration & dosage , Apamin/adverse effects , Apamin/pharmacology , Bacterial Infections/microbiology , Bacterial Infections/prevention & control , Chemical Phenomena , Chemistry, Pharmaceutical , Drug Compounding , Hydrogels , Male , Rats, Wistar , Skin/drug effects , Skin/injuries , Skin/microbiology , Wound Healing/immunology , Wounds, Penetrating/complications , Wounds, Penetrating/drug therapy , Wounds, Penetrating/immunology , Wounds, Penetrating/microbiology
14.
PLoS One ; 8(11): e78727, 2013.
Article in English | MEDLINE | ID: mdl-24236040

ABSTRACT

Nitric oxide (NO) is an unconventional membrane-permeable messenger molecule that has been shown to play various roles in the nervous system. How NO modulates ion channels to affect neuronal functions is not well understood. In gastropods, NO has been implicated in regulating the feeding motor program. The buccal motoneuron, B19, of the freshwater pond snail Helisoma trivolvis is active during the hyper-retraction phase of the feeding motor program and is located in the vicinity of NO-producing neurons in the buccal ganglion. Here, we asked whether B19 neurons might serve as direct targets of NO signaling. Previous work established NO as a key regulator of growth cone motility and neuronal excitability in another buccal neuron involved in feeding, the B5 neuron. This raised the question whether NO might modulate the electrical activity and neuronal excitability of B19 neurons as well, and if so whether NO acted on the same or a different set of ion channels in both neurons. To study specific responses of NO on B19 neurons and to eliminate indirect effects contributed by other cells, the majority of experiments were performed on single cultured B19 neurons. Addition of NO donors caused a prolonged depolarization of the membrane potential and an increase in neuronal excitability. The effects of NO could mainly be attributed to the inhibition of two types of calcium-activated potassium channels, apamin-sensitive and iberiotoxin-sensitive potassium channels. NO was found to also cause a depolarization in B19 neurons in situ, but only after NO synthase activity in buccal ganglia had been blocked. The results suggest that NO acts as a critical modulator of neuronal excitability in B19 neurons, and that calcium-activated potassium channels may serve as a common target of NO in neurons.


Subject(s)
Motor Neurons/physiology , Nitric Oxide/physiology , Potassium Channels, Calcium-Activated/metabolism , 4-Aminopyridine/pharmacology , Action Potentials , Animals , Apamin/pharmacology , Calcium Channels/metabolism , Cells, Cultured , Ganglia, Autonomic/cytology , Growth Cones/physiology , Helix, Snails , Nitric Oxide Donors/pharmacology , Patch-Clamp Techniques , Peptides/pharmacology , Potassium Channel Blockers/pharmacology , Potassium Channels, Calcium-Activated/agonists , Tetraethylammonium/pharmacology
15.
PLoS One ; 8(4): e61700, 2013.
Article in English | MEDLINE | ID: mdl-23637888

ABSTRACT

Bee venom has recently been suggested to possess beneficial effects in the treatment of Parkinson disease (PD). For instance, it has been observed that bilateral acupoint stimulation of lower hind limbs with bee venom was protective in the acute 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model of PD. In particular, a specific component of bee venom, apamin, has previously been shown to have protective effects on dopaminergic neurons in vitro. However, no information regarding a potential protective action of apamin in animal models of PD is available to date. The specific goals of the present study were to (i) establish that the protective effect of bee venom for dopaminergic neurons is not restricted to acupoint stimulation, but can also be observed using a more conventional mode of administration and to (ii) demonstrate that apamin can mimic the protective effects of a bee venom treatment on dopaminergic neurons. Using the chronic mouse model of MPTP/probenecid, we show that bee venom provides sustained protection in an animal model that mimics the chronic degenerative process of PD. Apamin, however, reproduced these protective effects only partially, suggesting that other components of bee venom enhance the protective action of the peptide.


Subject(s)
Apamin/pharmacology , Bee Venoms/pharmacology , Dopaminergic Neurons/drug effects , Neuroprotective Agents/pharmacology , Parkinson Disease/prevention & control , Acupuncture Points , Animals , Behavior, Animal/drug effects , Corpus Striatum/drug effects , Corpus Striatum/metabolism , Disease Models, Animal , Exploratory Behavior/drug effects , MPTP Poisoning/prevention & control , Male , Mice , Mice, Inbred C57BL , Tumor Necrosis Factor-alpha/metabolism
16.
J Neurosci ; 32(46): 16345-59, 2012 Nov 14.
Article in English | MEDLINE | ID: mdl-23152617

ABSTRACT

Mouse chromaffin cells (MCCs) fire spontaneous action potentials (APs) at rest. Ca(v)1.3 L-type calcium channels sustain the pacemaker current, and their loss results in depolarized resting potentials (V(rest)), spike broadening, and remarkable switches into depolarization block after BayK 8644 application. A functional coupling between Ca(v)1.3 and BK channels has been reported but cannot fully account for the aforementioned observations. Here, using Ca(v)1.3(-/-) mice, we investigated the role of Ca(v)1.3 on SK channel activation and how this functional coupling affects the firing patterns induced by sustained current injections. MCCs express SK1-3 channels whose tonic currents are responsible for the slow irregular firing observed at rest. Percentage of frequency increase induced by apamin was found inversely correlated to basal firing frequency. Upon stimulation, MCCs build-up Ca(v)1.3-dependent SK currents during the interspike intervals that lead to a notable degree of spike frequency adaptation (SFA). The major contribution of Ca(v)1.3 to the subthreshold Ca(2+) charge during an AP-train rather than a specific molecular coupling to SK channels accounts for the reduced SFA of Ca(v)1.3(-/-) MCCs. Low adaptation ratios due to reduced SK activation associated with Ca(v)1.3 deficiency prevent the efficient recovery of Na(V) channels from inactivation. This promotes a rapid decline of AP amplitudes and facilitates early onset of depolarization block following prolonged stimulation. Thus, besides serving as pacemaker, Ca(v)1.3 slows down MCC firing by activating SK channels that maintain Na(V) channel availability high enough to preserve stable AP waveforms, even upon high-frequency stimulation of chromaffin cells during stress responses.


Subject(s)
Adaptation, Physiological/physiology , Calcium Channels, L-Type/physiology , Chromaffin Cells/physiology , Small-Conductance Calcium-Activated Potassium Channels/physiology , Action Potentials/physiology , Adaptation, Physiological/drug effects , Animals , Apamin/pharmacology , Calcium/pharmacology , Calcium Channels/drug effects , Calcium Channels/physiology , Calcium Channels, L-Type/drug effects , Chromaffin Cells/drug effects , DNA, Complementary/biosynthesis , DNA, Complementary/genetics , Electrophysiological Phenomena , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Patch-Clamp Techniques , RNA/biosynthesis , RNA/isolation & purification , Real-Time Polymerase Chain Reaction , Small-Conductance Calcium-Activated Potassium Channels/drug effects , Sodium Channels/drug effects
17.
Am J Physiol Gastrointest Liver Physiol ; 303(9): G1059-66, 2012 Nov 01.
Article in English | MEDLINE | ID: mdl-22917628

ABSTRACT

The Japanese Kampo medicines Hange-shashin-to (TJ-14) and Keishi-ka-shakuyaku-to (TJ-60) have been used to treat symptoms of human diarrhea on an empirical basis as Japanese traditional medicines. However, it remains unclear how these drugs affect smooth muscle tissues in the distal colon. The aim of the present study was to investigate the effects of TJ-14 and TJ-60 on the contractile activity of circular smooth muscle from the rat distal colon. TJ-14 and TJ-60 (both 1 mg/ml) inhibited spontaneous contractions of circumferentially cut preparations with the mucosa intact. Blockade of nitric oxide (NO) synthase or soluble guanylate cyclase activity abolished the inhibitory effects of TJ-60 but only attenuated the inhibitory effects of TJ-14. Apamin (1 µM), a blocker of small-conductance Ca(2+)-activated K(+) channels (SK channels), attenuated the inhibitory effects of 5 mg/ml TJ-60 but not those of 5 mg/ml TJ-14. TJ-14 suppressed contractile responses (phasic contractions and off-contractions) evoked by transmural nerve stimulation and increased basal tone, whereas TJ-60 had little effect on these parameters. These results suggest that 1 mg/ml TJ-14 or TJ-60 likely inhibits spontaneous contractions of the rat distal colon through the production of NO. Activation of SK channels seems to be involved in the inhibitory effects of 5 mg/ml TJ-60. Since TJ-14 has potent inhibitory effects on myogenic and neurogenic contractile activity, TJ-14 may be useful in suppressing gastrointestinal motility.


Subject(s)
Drugs, Chinese Herbal , Gastrointestinal Motility/drug effects , Muscle Contraction/drug effects , Muscle, Smooth/drug effects , Small-Conductance Calcium-Activated Potassium Channels/metabolism , Animals , Antidiarrheals/pharmacokinetics , Apamin/pharmacology , Biological Availability , Colon/physiopathology , Drugs, Chinese Herbal/administration & dosage , Drugs, Chinese Herbal/pharmacokinetics , Guanylate Cyclase/metabolism , Humans , Male , Medicine, Kampo , Muscle, Smooth/physiopathology , Nitric Oxide Synthase/metabolism , Rats , Rats, Wistar
18.
J Pharmacol Exp Ther ; 339(3): 842-50, 2011 Dec.
Article in English | MEDLINE | ID: mdl-21880870

ABSTRACT

This study was designed to investigate whether calcium-activated potassium channels of small (SK(Ca) or K(Ca)2) and intermediate (IK(Ca) or K(Ca)3.1) conductance activated by 6,7-dichloro-1H-indole-2,3-dione 3-oxime (NS309) are involved in both nitric oxide (NO) and endothelium-derived hyperpolarizing factor (EDHF)-type relaxation in large and small rat mesenteric arteries. Segments of rat superior and small mesenteric arteries were mounted in myographs for functional studies. NO was recorded using NO microsensors. SK(Ca) and IK(Ca) channel currents and mRNA expression were investigated in human umbilical vein endothelial cells (HUVECs), and calcium concentrations were investigated in both HUVECs and mesenteric arterial endothelial cells. In both superior (∼1093 µm) and small mesenteric (∼300 µm) arteries, NS309 evoked endothelium- and concentration-dependent relaxations. In superior mesenteric arteries, NS309 relaxations and NO release were inhibited by both N(G),N(G)-asymmetric dimethyl-l-arginine (ADMA) (300 µM), an inhibitor of NO synthase, and apamin (0.5 µM) plus 1-[(2-chlorophenyl)diphenylmethyl]-1H-pyrazole (TRAM-34) (1 µM), blockers of SK(Ca) and IK(Ca) channels, respectively. In small mesenteric arteries, NS309 relaxations were reduced slightly by ADMA, whereas apamin plus an IK(Ca) channel blocker almost abolished relaxation. Iberiotoxin did not change NS309 relaxation. HUVECs expressed mRNA for SK(Ca) and IK(Ca) channels, and NS309 induced increases in calcium, outward current, and NO release that were blocked by apamin and TRAM-34 or charybdotoxin. These findings suggest that opening of SK(Ca) and IK(Ca) channels leads to endothelium-dependent relaxation that is mediated mainly by NO in large mesenteric arteries and by EDHF-type relaxation in small mesenteric arteries. NS309-induced calcium influx appears to contribute to the formation of NO.


Subject(s)
Biological Factors/physiology , Indoles/pharmacology , Mesenteric Arteries/drug effects , Nitric Oxide/metabolism , Oximes/pharmacology , Potassium Channels, Calcium-Activated/physiology , Vasodilation , Vasodilator Agents/pharmacology , Animals , Anthracenes/pharmacology , Apamin/pharmacology , Arginine/analogs & derivatives , Arginine/pharmacology , Drug Evaluation, Preclinical , Human Umbilical Vein Endothelial Cells , Male , Mesenteric Arteries/physiology , Nitric Oxide Synthase/antagonists & inhibitors , Potassium Channels, Calcium-Activated/agonists , Propane/analogs & derivatives , Propane/pharmacology , Pyrazoles/pharmacology , Rats , Rats, Wistar
19.
Planta Med ; 76(16): 1814-9, 2010 Nov.
Article in English | MEDLINE | ID: mdl-20506075

ABSTRACT

Anemoside A(3), a lupane-type triterpenoid saponin, exists in the roots of Pulsatilla chinensis, but its pharmacological properties are largely unknown. The present study aimed to investigate the mechanisms underlying anemoside A(3)-induced relaxation in rat renal arteries. Changes of isometric force were determined on arteries with a myograph. Anemoside A(3) caused concentration-dependent relaxation in precontracted aortas, mesenteric, left coronary, and renal arteries. Removal of endothelium or treatment with charybdotoxin plus apamin slightly but significantly attenuated the relaxation in renal arteries. TEA(+) inhibited the relaxation caused by anemoside A(3) in renal arteries with and without endothelium while glibenclamide, BaCl(2), or capsaicin had no effect on it. Anemoside A(3) produced less relaxation in rings contracted by 60 mM KCl compared with rings contracted by receptor-dependent constrictors. It further inhibited contractions induced by Ca(2+) influx through nifedipine-sensitive voltage-gated Ca(2+) channels, nifedipine-insensitive receptor-operated Ca(2+) channels, and by intracellular Ca(2+) release. Pretreatment with nifedipine attenuated anemoside A(3)-induced relaxation. Taken together, the present results indicate that anemoside A(3) produces relaxation in rat renal arteries through multiple mechanisms. The release of CTX/apamin-sensitive endothelium-derived hyperpolarizing factor, stimulation of TEA(+)-sensitive K(+) channel, and inhibition of Ca(2+) influx jointly contribute to the relaxation.


Subject(s)
Calcium Channel Blockers/pharmacology , Endothelium, Vascular/metabolism , Muscle, Smooth, Vascular/drug effects , Plant Extracts/pharmacology , Pulsatilla/chemistry , Saponins/pharmacology , Triterpenes/pharmacology , Vasodilator Agents/pharmacology , Animals , Apamin/pharmacology , Arteries/drug effects , Biological Factors/metabolism , Calcium/metabolism , Calcium Channel Blockers/isolation & purification , Capsaicin/pharmacology , Charybdotoxin/pharmacology , Dose-Response Relationship, Drug , Female , Glyburide/pharmacology , Kidney/blood supply , Kidney/drug effects , Male , Muscle Contraction/drug effects , Myography , Nifedipine , Plant Extracts/chemistry , Plant Roots , Potassium Channels/drug effects , Potassium Chloride , Rats , Rats, Sprague-Dawley , Saponins/isolation & purification , Tetraethylammonium/pharmacology , Triterpenes/isolation & purification , Vasodilator Agents/isolation & purification
20.
Am J Physiol Gastrointest Liver Physiol ; 298(5): G755-63, 2010 May.
Article in English | MEDLINE | ID: mdl-20167876

ABSTRACT

The relaxant effects of Rikkunshi-to (TJ-43), a gastroprotective herbal medicine, on rat gastric fundus were investigated. Experiments were carried out using standard tension and intracellular microelectrode recording techniques. During contraction induced by enprostil (0.5 microM), a prostaglandin E(2) analog, TJ-43, produced relaxation dose dependently (0.1-5.0 mg/ml) in the rat fundic circular smooth muscle (CSM) strips. The relaxant effects of TJ-43 were not affected by tetrodotoxin or 1 H[1, 2, 4] oxadiazolo [4, 3-a] quinoxalin-1-one (10 microM), an inhibitor of soluble guanylate cyclase. TJ-43 inhibited enprostil-induced membrane depolarization. Apamin (1 microM), a blocker of small-conductance Ca(2+)-activated K(+) (SK) channel, inhibited T-43-induced membrane repolarization. TJ-43-induced relaxation was biphasic, comprising of an initial fast followed by a second slow relaxation. The fast relaxation was abolished by apamin. Application of high K(+) (29.4 mM [K(+)](o)) also abolished the fast relaxation induced by TJ-43. In diabetic Goto-Kakizaki (GK) rat fundic CSM strips, the relaxant responses of TJ-43 during enprostil-induced contraction were increased compared with control rat strips. These results indicate that TJ-43 elicited fast muscle relaxation through membrane hyperpolarization induced by the activation of SK channels; the time-dependent slow relaxation reflects an additional direct of TJ-43 on CSM in the rat gastric fundus. Because TJ-43-evoked relaxation of fundic CSM strips was more potent in diabetic GK rat than in control rat, further analysis of this herb could lead to better treatments of diabetic gastroparesis.


Subject(s)
Drugs, Chinese Herbal/pharmacology , Gastric Fundus/drug effects , Muscle Relaxation/drug effects , Animals , Apamin/pharmacology , Enprostil/antagonists & inhibitors , Hesperidin/pharmacology , Membrane Potentials/drug effects , Membrane Potentials/physiology , Muscle, Smooth/drug effects , Oxadiazoles/pharmacology , Potassium/administration & dosage , Pyrimidinones/pharmacology , Quinoxalines/pharmacology , Rats , Thiazoles/pharmacology , Verapamil/pharmacology
SELECTION OF CITATIONS
SEARCH DETAIL