ABSTRACT
Two unprecedented benzoxepins were obtained from the ethyl acetate fraction of the leaves of Rhizophora annamalayana Kathir, and characterized as 4-(11-(hydroxymethyl)-10-methylpentan-2-yl)-4, 5-dihydrobenzo[c]oxepin-1(3H)-one (1) and (E)-methyl-14-hydroxy-4-(11-(5-hydroxy-1-oxo-3,4,5-tetrahydrobenzo[c]oxepin-4-yl)ethyl)-10-methylhept-11-enoate (2). The benzoxepin 2 exhibited greater 1, 1-diphenyl-2-picrylhydrazyl and 2, 2'-azino-bis-3 ethylbenzothiozoline-6-sulfonic acid diammonium radical scavenging assays (IC50 0.68 and 0.84 mg/mL, respectively) than those recorded with 1 (IC50 0.70 and 0.89 mg/mL, respectively). The tetrahydrobenzo[c]oxepin analogue (2) exhibited significantly great cyclooxygenase-2 and 5-lipoxygenase inhibitory properties (IC50 0.87 and 0.94 mg/mL, respectively), while compared with its dihydrobenzo[c]oxepin-1(3H)-one isoform (1) (IC50 1.16 and 1.64 mg/mL, respectively). The dihydrobenzo[c]oxepin-1(3H)-one isoform (2) exhibited significantly greater selectivity index (~2) than synthetic ibuprofen (0.44) (p < 0.05), which attributed the higher anti-inflammatory selectivity of the former against inducible pro-inflammatory cyclooxygenase-2 than its constitutive isoform (cyclooxygenase-1). No significant difference in 5-lipoxygenase (5-LOX) inhibitory activities were apparent between compound 2 (IC50 0.94 mg/mL) and synthetic ibuprofen (IC50 0.93 mg/mL).
Subject(s)
Benzoxepins/isolation & purification , Cyclooxygenase 2 Inhibitors/isolation & purification , Lipoxygenase Inhibitors/isolation & purification , Rhizophoraceae/chemistry , Antioxidants/pharmacology , Benzoxepins/chemistry , Benzoxepins/pharmacology , Cyclooxygenase 2 Inhibitors/chemistry , Cyclooxygenase 2 Inhibitors/pharmacology , Lipoxygenase Inhibitors/chemistry , Lipoxygenase Inhibitors/pharmacology , Plant Extracts/analysis , Plant Leaves/chemistryABSTRACT
Cortex Dictamni is a commonly-used traditional Chinese herbal medicine for the treatment of skin inflammation, tinea, and eczema. Recently, some studies reported that Cortex Dictamni might induce liver injury, suggesting more attention to its safety. The current study was designed to investigate subchronic toxicity of Cortex Dictamni aqueous extract (CDAE) and ethanol extract (CDEE) in mice and the potential hepatotoxicity mechanisms in vitro. Firstly, CDAE or CDEE groups were administrated with varying dosages (2.3, 4.6, or 9.2 g/kg/day, p.o.) in mice for 28 days in subchronic toxicity studies. General clinical signs and biochemical parameters were examined, and morphological analyses were conducted. Secondly, we identified the different constituents of CDAE and CDEE using HPLC-MS/MS and chose major components for further study. In order to determine the toxic components, we investigated the cytotoxicity of extracts and chosen components using CCK-8 assay in HepG2 cells. Furthermore, we explored the possible hepatotoxicity mechanisms of Cortex Dictamni using a high content analysis (HCA). The results showed that no significant differences of general clinical signs were observed in mice. Aspartate alanine aminotransferase (ALT) and aminotransferase (AST) were significantly increased in the high-dose CDAE and CDEE groups compared to the control group. Meanwhile, the absolute and relative liver weights and liver/brain ratio were significantly elevated, and histological examination of liver demonstrated cellular enlargement or nuclear shrinkage. In UPLC analysis, we compared the chemical constituents between CDAE and CDEE, and chose dictamnine, obakunone, and fraxinellone for hepatotoxicity evaluation in the in vitro studies. In the CCK-8 assay, CDAE, CDEE, dictamnine, obakunone, and fraxinellone decreased the cell viability in a dose-dependent manner after treatment for 48 h. Furthermore, the cell number decreased, while the nuclear intensity, cell membrane permeability, and concentration of reactive oxygen species were shown to increase, meanwhile, mitochondrial membrane potential was also changed in HepG2 cells following 48 h of compounds treatment using HCA. Our studies suggested that CDAE and CDEE have potential hepatotoxicity, and that the alcohol extraction process could increase toxicity. Dictamnine, obakunone, and fraxinellone may be the possible toxic components in Cortex Dictamni with dictamnine as the most potentially hepatotoxic component, whose potential hepatotoxicity mechanism may be associated with cell apoptosis. Moreover, this study could provide valuable data for clinical drug safety research of Cortex Dictamni and a good example for safety study of other Chinese herbal medicines.
Subject(s)
Chemical and Drug Induced Liver Injury/metabolism , Dictamnus/chemistry , Drugs, Chinese Herbal/chemistry , Drugs, Chinese Herbal/toxicity , Animals , Apoptosis/drug effects , Benzofurans/chemistry , Benzofurans/toxicity , Benzoxepins/chemistry , Benzoxepins/toxicity , Cell Count , Cell Survival/drug effects , Chemical and Drug Induced Liver Injury/etiology , Chemical and Drug Induced Liver Injury/pathology , Ethanol/chemistry , Female , Hep G2 Cells , Humans , Limonins/chemistry , Limonins/toxicity , Liver/drug effects , Liver/metabolism , Liver/pathology , Male , Membrane Potential, Mitochondrial/drug effects , Mice , Mice, Inbred ICR , Quinolines/chemistry , Quinolines/toxicity , Toxicity Tests, Subchronic , Water/chemistryABSTRACT
Two new dibenz[b,f]oxepins, empetroxepins A and B (1 and 2), and seven known compounds (3-9) were isolated from an extract of the Canadian medicinal plant Empetrum nigrum that significantly inhibited the growth of Mycobacterium tuberculosis H37Ra. The structures of 1 and 2 were established through analysis of NMR and MS data. The antimycobacterial activity of the plant extract was attributed primarily to the presence of two chalcone derivatives (6 and 7) that exhibited selective antimycobacterial activity (IC50 values of 23.8 and 32.8 µM, respectively) in comparison to mammalian (HEK 293) cells (IC50 values of 109 and 249 µM, respectively).
Subject(s)
Antitubercular Agents/isolation & purification , Antitubercular Agents/pharmacology , Benzoxepins/isolation & purification , Benzoxepins/pharmacology , Chalcone/isolation & purification , Chalcone/pharmacology , Ericaceae/chemistry , Mycobacterium tuberculosis/drug effects , Oxepins/isolation & purification , Oxepins/pharmacology , Animals , Antitubercular Agents/chemistry , Benzoxepins/chemistry , Canada , Chalcone/chemistry , HEK293 Cells , Humans , Inhibitory Concentration 50 , Microbial Sensitivity Tests , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Oxepins/chemistryABSTRACT
The hERG channel is an important antitarget in safety pharmacology. Several drugs have been withdrawn from the market or received severe usage restrictions because of hERG-related cardiotoxicity. In a screening of medicinal plants for hERG channel inhibition using a two-microelectrode voltage clamp assay with Xenopus laevis oocytes, a dichloromethane extract of the roots of Gnidia polycephala reduced the peak tail hERG current by 58.8 ± 13.4% (n = 3) at a concentration of 100 µg/mL. By means of HPLC-based activity profiling daphnane-type diterpenoid orthoesters (DDOs) 1, 4, and 5 were identified as the active compounds [55.4 ± 7.0% (n = 4), 42.5 ± 16.0% (n = 3), and 51.3 ± 9.4% (n = 4), respectively, at 100 µM]. In a detailed phytochemical profiling of the active extract, 16 compounds were isolated and characterized, including two 2-phenylpyranones (15 and 16) with an unprecedented tetrahydro-4H-5,8-epoxypyrano[2,3-d]oxepin-4-one skeleton, two new DDOs (3 and 4), two new guaiane sesquiterpenoids (11 and 12), and 10 known compounds (1, 2, 5-10, 13, and 14). Structure elucidation was achieved by extensive spectroscopic analysis (1D and 2D NMR, HRMS, and electronic circular dichroism), computational methods, and X-ray crystallography.
Subject(s)
Benzoxepins/isolation & purification , Benzoxepins/pharmacology , Diterpenes/isolation & purification , Diterpenes/pharmacology , Ether-A-Go-Go Potassium Channels/antagonists & inhibitors , Thymelaeaceae/chemistry , Animals , Benzoxepins/chemistry , Chromatography, High Pressure Liquid , Crystallography, X-Ray , Diterpenes/chemistry , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Oocytes/drug effects , South Africa , XenopusABSTRACT
Here we have prepared a series of ester compounds of obacunone, a naturally occurring limonoid, isolated from plants such as Citrus and Dictamnus angustifolius. Their insecticidal activity was evaluated at 1 mg/mL against the pre-third-instar larvae of oriental armyworm (Mythimna separata Walker), a typical lepidopteran pest. When obacunone reacted with NaBH4, the ratio of two reduction products, C7α-hydroxyobacunone (2) and C7ß-hydroxyobacunone (3), was related to the reaction mixing solvents. C7α-Propionyloxybacunone (4b) and C7ß-(n)heptanoyloxybacunone (5g) exhibited the more promising insecticidal activity than their precursor obacunone and toosendanin.
Subject(s)
Benzoxepins/chemistry , Drugs, Chinese Herbal/chemistry , Insecticides/chemistry , Limonins/chemistry , Melia azedarach , Moths/drug effects , Animals , Benzoxepins/isolation & purification , Benzoxepins/pharmacology , Crystallography, X-Ray , Drugs, Chinese Herbal/pharmacology , Esters , Insecticides/isolation & purification , Insecticides/pharmacology , Larva/drug effects , Limonins/isolation & purification , Limonins/pharmacology , Oxidation-ReductionABSTRACT
Cortex Phellodendri is a typical Chinese herb with a large number of alkaloids existing in all parts of it. The most common methods for screening and isolating alkaloids are mostly labor intensive and time consuming. In this study, a new assay based upon ultrafiltration liquid chromatography was developed for the rapid screening of ligands for α-glucosidase and xanthine oxidase. The C. Phellodendri extract was found to contain two alkaloids with both α-glucosidase- and xanthine oxidase binding activities and one lactone with α-glucosidase-binding activity. Subsequently, with the help of high-speed countercurrent chromatography, the specific binding ligands including palmatine, berberine, and obaculactone with purities of 97.38, 96.12, and 96.08%, respectively, were successfully separated. An optimized low-toxicity two-phase solvent system composed of ethyl acetate/n-butanol/ethanol/water (3.5:1.7:0.5:5, v/v/v/v) was used to isolate the three compounds mentioned above from C. Phellodendri. The targeted compounds were identified by liquid chromatography coupled with mass spectrometry and NMR spectroscopy. Therefore, ultrafiltration liquid chromatography combined with high-speed countercurrent chromatography is not only a powerful tool for screening and isolating α-glucosidase and xanthine oxidase inhibitors in complex samples but is also a useful platform for discovering bioactive compounds for the prevention and treatment of diabetes mellitus and gout.
Subject(s)
Drugs, Chinese Herbal/chemistry , Enzyme Inhibitors/isolation & purification , Glycoside Hydrolase Inhibitors/isolation & purification , Phellodendron/chemistry , Xanthine Oxidase/antagonists & inhibitors , alpha-Glucosidases/metabolism , Benzoxepins/chemistry , Benzoxepins/isolation & purification , Berberine/chemistry , Berberine/isolation & purification , Berberine Alkaloids/chemistry , Berberine Alkaloids/isolation & purification , Chromatography, High Pressure Liquid , Countercurrent Distribution , Enzyme Inhibitors/chemistry , Glycoside Hydrolase Inhibitors/chemistry , Limonins/chemistry , Limonins/isolation & purification , Molecular Structure , UltrafiltrationABSTRACT
To analyze components of Citrus reticulata and salt-processed C. reticulata by ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF/MS), and compared the changes in components before and after being processed with salt. Principal component analysis (PCA) and partial least squares discriminant analysis (OPLS-DA) were adopted to analyze the difference in fingerprint between crude and processed C. reticulata, showing increased content of eriocitrin, limonin, nomilin and obacunone increase in salt-processed C. reticulata. Potential chemical markers were identified as limonin, obacunone and nomilin, which could be used for distinguishing index components of crude and processed C. reticulata.
Subject(s)
Citrus/chemistry , Salts/chemistry , Benzoxepins/chemistry , Chromatography, High Pressure Liquid/methods , Discriminant Analysis , Limonins/chemistry , Mass Spectrometry/methods , Principal Component Analysis/methodsABSTRACT
A series of suitable five-membered heterocyclic alternatives to thiophenes within a thienobenzoxepin class of PI3-kinase (PI3K) inhibitors was discovered. Specific thiazolobenzoxepin 8-substitution was identified that increased selectivity over PI3Kß. PI3Kß-sparing compound 27 (PI3Kß Ki,app/PI3Kα Ki,app=57) demonstrated dose-dependent knockdown of pAKT, pPRAS40 and pS6RP in vivo as well as differential effects in an in vitro proliferation cell line screen compared to pan PI3K inhibitor GDC-0941. A new structure-based hypothesis for reducing inhibition of the PI3K ß isoform while maintaining activity against α, δ and γ isoforms is presented.
Subject(s)
Benzoxepins/chemistry , Enzyme Inhibitors/chemistry , Phosphoinositide-3 Kinase Inhibitors , Thiazoles/chemistry , Benzoxepins/chemical synthesis , Benzoxepins/pharmacology , Binding Sites , Cell Proliferation/drug effects , Drug Evaluation, Preclinical , Enzyme Inhibitors/chemical synthesis , Enzyme Inhibitors/pharmacology , Humans , MCF-7 Cells , Molecular Docking Simulation , Phosphatidylinositol 3-Kinase/metabolism , Protein Isoforms/antagonists & inhibitors , Protein Isoforms/metabolism , Protein Structure, Tertiary , Proto-Oncogene Proteins c-akt/metabolism , Structure-Activity RelationshipABSTRACT
CONTEXT: Lichens have been used for various purposes such as dyes, perfumes and remedies in folk medicine indicating the pharmaceutical potential of lichens. OBJECTIVE: Lichen growth in nature is very slow. To overcome this major drawback, we standardized the culture media to culture the lichen Usnea complanata (Müll.Arg.) Motyka (Parmeliaceae) for (1) in vitro synthesis of natural lichen substances, and (2) determination of antioxidative and cardiovascular-protective activity of usnic acid and psoromic acid. MATERIALS AND METHODS: Lichen U. complanata has been cultured in fermentor under submerged condition. Antioxidative and cardiovascular-protective activity of the extract and the purified lichen substances usnic and psoromic acid have been determined. RESULTS: Except methanol, all other extracts exhibited antioxidative action in terms of free radical scavenging activity (FRSA) with a half-inhibiting concentration (IC50) value of 22.86 to 25.0 µg/mL, nitric oxide radical scavenging activity (NORSA) 141.3 to 149.1 µg/mL and for lipid peroxidation inhibition (LPI) 125 to 157.9 µg/mL. Usnic acid or psoromic acid showed antioxidative action with IC50 values ranging from 0.174 to 0.271 mg/mL. Methanol and ethyl acetate extract showed hydroxy-3-methyl-glutaryl-CoA reductase (HMGR) inhibition of 65.18 to 74.81%. Only 43.47% inhibition of angiotensin converting enzyme (ACE) was shown by methanol extract. Usnic acid showed noncompetitive type of HMGR inhibition and uncompetitive type of ACE inhibition. Psoromic acid exhibited competitive type of HMGR inhibition and mixed type of ACE inhibition. DISCUSSION: U. complanata showed both cardiovascular-protective and antioxidant properties. The lichen species U. complanata may be a natural bioresource for possible pharmaceutical applications.
Subject(s)
Angiotensin-Converting Enzyme Inhibitors/pharmacology , Antioxidants/pharmacology , Benzofurans/pharmacology , Benzoxepins/pharmacology , Carboxylic Acids/pharmacology , Drug Discovery , Hydroxymethylglutaryl-CoA Reductase Inhibitors/pharmacology , Usnea/metabolism , Angiotensin-Converting Enzyme Inhibitors/chemistry , Angiotensin-Converting Enzyme Inhibitors/isolation & purification , Angiotensin-Converting Enzyme Inhibitors/metabolism , Antioxidants/chemistry , Antioxidants/isolation & purification , Antioxidants/metabolism , Benzofurans/chemistry , Benzofurans/isolation & purification , Benzofurans/metabolism , Benzoxepins/chemistry , Benzoxepins/isolation & purification , Benzoxepins/metabolism , Biological Products/chemistry , Biological Products/isolation & purification , Biological Products/metabolism , Biological Products/pharmacology , Bioreactors , Carboxylic Acids/chemistry , Carboxylic Acids/isolation & purification , Carboxylic Acids/metabolism , Culture Media, Conditioned/chemistry , Culture Media, Conditioned/metabolism , Ethnopharmacology , Fermentation , Free Radical Scavengers/chemistry , Free Radical Scavengers/isolation & purification , Free Radical Scavengers/metabolism , Free Radical Scavengers/pharmacology , Hydroxymethylglutaryl-CoA Reductase Inhibitors/chemistry , Hydroxymethylglutaryl-CoA Reductase Inhibitors/isolation & purification , Hydroxymethylglutaryl-CoA Reductase Inhibitors/metabolism , India , Kinetics , Lipid Peroxidation/drug effects , Nitric Oxide/antagonists & inhibitors , Osmolar Concentration , Solubility , Solvents/chemistry , Usnea/growth & developmentABSTRACT
The construction of a EP(4) antagonists pharmacophore model and the discovery of a highly potent oxepinic series of EP(4) antagonists is discussed. Compound 1a exhibits an excellent selectivity profile toward EP(2) receptor subtype and low cytochrome P450 inhibition potential.
Subject(s)
Benzoxepins/chemical synthesis , Benzoxepins/pharmacology , Models, Molecular , Receptors, Prostaglandin E, EP4 Subtype/antagonists & inhibitors , Benzoxepins/chemistry , Drug Evaluation, Preclinical , Humans , In Vitro TechniquesABSTRACT
HPLC-SPE-NMR analysis of a crude extract of fermentation broth of cultured PESTALOTIOPSIS VIRGATULA isolate TC-320 from TERMINALIA CHEBULA Retz. (Combretaceae) disclosed the presence of a simple but unprecedented low-molecular-weight metabolite, 9-hydroxybenzo[ C]oxepin-3[1 H]-one, subsequently isolated by a targeted purification procedure.
Subject(s)
Ascomycota/chemistry , Benzoxepins/chemistry , Chromatography, High Pressure Liquid/methods , Magnetic Resonance Spectroscopy/methodsABSTRACT
Eleven new secondary metabolites (1-11), together with two known flavanones (12 and 13) and five known bibenzyls (14-18), were isolated from the root extract of Bauhinia purpurea. New compounds include eight dihydrodibenzoxepins (1-8), a dihydrobenzofuran (9), a novel spirochromane-2,1'-hexenedione (10), and a new bibenzyl (11). Antimycobacterial, antimalarial, antifungal, cytotoxic, and anti-inflammatory activities of the isolated compounds are reported, and biosynthetic pathways of these compounds are also discussed.
Subject(s)
Anti-Bacterial Agents/isolation & purification , Anti-Bacterial Agents/pharmacology , Anti-Inflammatory Agents/isolation & purification , Anti-Inflammatory Agents/pharmacology , Antifungal Agents/isolation & purification , Antifungal Agents/pharmacology , Antimalarials/isolation & purification , Antimalarials/pharmacology , Bauhinia/chemistry , Benzoxepins/isolation & purification , Benzoxepins/pharmacology , Bibenzyls/isolation & purification , Bibenzyls/pharmacology , Flavanones/isolation & purification , Flavanones/pharmacology , Plants, Medicinal/chemistry , Spiro Compounds/isolation & purification , Spiro Compounds/pharmacology , Animals , Anti-Bacterial Agents/chemistry , Anti-Inflammatory Agents/chemistry , Antifungal Agents/chemistry , Antimalarials/chemistry , Benzoxepins/chemistry , Bibenzyls/chemistry , Flavanones/chemistry , Microbial Sensitivity Tests , Molecular Structure , Plant Roots/chemistry , Spiro Compounds/chemistry , ThailandABSTRACT
Bioassay-guided (P388 lymphocytic leukemia cell line) separation of extracts prepared from the leaves, stems, and pods of Bauhinia purpurea, and, in parallel, its roots, led to the isolation of four new dibenz[b,f]oxepins (2a, 3-5) named bauhiniastatins 1-4, as well as the known and related pacharin (1) as cancer cell growth inhibitors. The occurrence of oxepin derivatives in nature is quite rare. Bauhiniastatins 1-4 were found to exhibit significant growth inhibition against a minipanel of human cancer cell lines, and bauhiniastatin 1 (2a) was also found to inhibit the P388 cancer cell line. Structures for these new cancer cell growth inhibitors were established by spectroscopic techniques that included HRMS and 2D NMR.
Subject(s)
Antineoplastic Agents, Phytogenic/isolation & purification , Bauhinia/chemistry , Benzoxepins/isolation & purification , Plants, Medicinal/chemistry , Animals , Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/pharmacology , Benzoxepins/chemistry , Benzoxepins/pharmacology , Drug Screening Assays, Antitumor , Humans , India , Leukemia P388 , Mice , Molecular Structure , Plant Roots/chemistry , Tumor Cells, CulturedABSTRACT
The antifeedant activity of Citrus-derived limonoids limonin (1), nomilin (2), and obacunone (3) and their semisynthetic derivatives 4-26 was evaluated against a commercially important pest, Spodoptera frugiperda. Simple chemical conversions were carried out on the natural limonoids obtained from seeds of Citrus limon. These conversions focused on functional groups considered to be important for the biological activity, namely the C-7 carbonyl and the furan ring. In particular, reduction at C-7 afforded the related alcohols, and from these their acetates, oximes, and methoximes were prepared. Hydrogenation of the furan ring was also performed on limonin and obacunone. The known antifeedant properties of the Citrus limonoids are confirmed. Comparison with previously reported data shows that insect species vary in their behavioral responses to these structural modifications. Highly significant antifeedant activity (P < 0.01) for two natural (1 and 3) and three semisynthetic limonoids (4, 8, and 10) was observed against S. frugiperda.
Subject(s)
Citrus/chemistry , Larva/drug effects , Limonins/pharmacology , Spodoptera/drug effects , Animals , Benzoxepins/chemistry , Benzoxepins/pharmacology , Eating/drug effects , Food Handling , Industrial Waste , Insecticides , Larva/physiology , Limonins/chemistry , Plant Extracts/chemistry , Structure-Activity RelationshipABSTRACT
Deoxyobacunone (1), a new limonoid with a double bond in ring D, has been isolated from the root bark of Harrisonia abyssinica collected in Nigeria. Also, the known limonoids obacunone (2), harrisonin (3), 12beta-acetoxyharrisonin (4), and pedonin (5) have been isolated. The structure of 1 was assigned unambiguously by spectral data analysis. Under laboratory conditions, 10(-3)-10(-5) M concentrations of compounds 1-5 exhibited significant stimulatory activity (12-98%) against conditioned Striga hermonthica seeds. This study provided useful insight regarding the functionalities required for activity of limonoids against Striga seeds. The variation in activity was rationalized through quantitative structure-activity relationship (QSAR) models based on several molecular descriptors including van der Waals volume (VDW(v)), molecular polarizability (alpha), dipole moment (mu), log P, and the differences between the highest occupied molecular orbital and lowest unoccupied molecular orbital (HOMO-LUMO gap).
Subject(s)
Benzopyrans/isolation & purification , Benzoxepins/isolation & purification , Furans/isolation & purification , Limonins , Plants, Medicinal/chemistry , Pyrones/isolation & purification , Scrophulariaceae/chemistry , Benzopyrans/chemistry , Benzopyrans/pharmacology , Benzoxepins/chemistry , Benzoxepins/pharmacology , Chemical Phenomena , Chemistry, Physical , Furans/chemistry , Furans/pharmacology , Models, Chemical , Molecular Conformation , Molecular Structure , Nigeria , Nuclear Magnetic Resonance, Biomolecular , Plant Roots/chemistry , Pyrones/chemistry , Pyrones/pharmacology , Scrophulariaceae/drug effects , Seeds/chemistry , Seeds/drug effects , Structure-Activity RelationshipABSTRACT
Two novel prenyl 3-benzoxepin derivatives, perilloxin (1) and dehydroperilloxin (2), were isolated from the dichloromethane extract of the stems of Perilla frutescens var. acuta. Their structures were elucidated as (-)-(R)-5-methoxy-2,3-dihydrofuro[2, 3-g][3]benzoxepin and 5-methoxyfuro[2,3-g][3]benzoxepin, respectively, based on UV, MS, (1)H and (13)C NMR, NOE, (1)H-(13)C COSY, and HMBC spectral data. They were isolated following bioassay-guided fractionation, using an in vitro cyclooxygenase-1 test. Compounds 1 and 2 possess inhibitory activities, with IC(50) values of 23.2 microM and 30.4 microM, respectively.