ABSTRACT
Objetivou-se relatar a experiência de discentes e docentes diante da aplicação de ferramentas de metodologia ativa para a integração dos temas de Bioquímica com os das disciplinas do eixo clínico-profissional. Inicialmente, as subturmas da aula prática foram divididas em equipes de trabalho. Os alunos receberam um protocolo contendo objetivos, princípios gerais e procedimentos na semana anterior à aula prática. Nos dias das aulas de "Preparo de Soluções" e "Capacidade Tamponante", um artigo científico relativo à Odontologia foi entregue para leitura e discussão em grupo. Como atividade prática, as equipes recebiam um desafio relacionado ao artigo e que exigiria aplicação dos objetivos de aula. Esta experiência demonstrou que a metodologia ativa pode funcionar como facilitadora para uma abordagem contextualizada e integrada da Bioquímica, refletindo em maior engajamento e rendimento dos alunos, além de contribuir para um aprendizado significativo (AU).
El objetivo fue relatar la experiencia de estudiantes y profesores en cuanto a la aplicación de herramientas metodológicas activas para la integración de los temas de Bioquímica con los de las disciplinas del eje clínico-profesional. Inicialmente, las subclases de la clase práctica se dividían en equipos de trabajo. Los estudiantes recibieron un protocolo con objetivos, principios generales y procedimientos en la semana anterior a la clase práctica. En los días de las clases de "Preparación de Soluciones" y "Capacidad Amortiguadora", se entregó un artículo científico relacionado con la Odontología para lectura y discusión en grupo. Como actividad práctica, los equipos recibieron un reto relacionado con el artículo y que requería la aplicación de los objetivos de clase. Esta experiencia demostró que la metodología activa puede funcionar como facilitadora de un abordaje contextualizado e integrado de la Bioquímica, reflejándose en un mayor compromiso y desempeño de los estudiantes, además de contribuir al aprendizaje significativo (AU).
The objective was to report the experience of students and professors regarding the application of active methodology tools aimed at integrating Biochemistry themes with those of the disciplines from the clinical-professional axis. Initially, subgroups forthe practical class were divided into work teams. The students received a protocol containing objectives, general principles and procedures the week before the practical class. On the days of the "Preparation of Solutions" and "Buffering Capacity" classes, a scientific article related to Dentistry was delivered for group reading and discussion. As a practical activity, the teams received a challenge related to the article,which would require application of the class objectives. This experience demonstrated that the active methodology can work as a facilitator for a contextualized and integrated approach to Biochemistry, reflecting in greater engagement and student performance, in addition to contributing to meaningful learning (AU).
Subject(s)
Biochemistry/education , Problem-Based Learning , Education, Dental , Students, Dental , Teaching , Faculty, DentalABSTRACT
Aims: it was evaluated the antioxidant effect of the ethanolic extract of Caesal-pinia ferrea bark in a model of oxidative stress induced by paracetamol (PCM). Methods: male Swiss mice were subdivided into four groups (control; PCM; PCM+extract; extract; n=8) in which a dose of paracetamol (250 mg.kg-1) was administered and after 3 hours the treatment with the extract (100 mg.kg-1/day) was administered for seven days, via gavage. Oxidative stress biomarkers were determined, such as catalase, glutathione-S-transferase, reduced gluta-thione, ascorbic acid, thiobarbituric acid reactive substances and carbonylated proteins of liver, kidneys and brain and plasma parameters through the dosage of glucose, cholesterol, triglycerides, aspartate aminotransferase and alanine aminotransferase. Results: the Caesalpinia ferrea extract was able to reverse the lipid and protein damage caused by the drug in the liver tissue and caused the same effect in the renal and brain tissues in the carbonylated proteins. The extract alone decreased liver glutathione-S-transferase and increased catalase and brain glutathione-S-transferase activity, in addition to lowering glucose and cholesterol, but without altering the triglycerides. Conclusions: it was possible to conclude that the ethanolic extract of the bark of Caesalpinia ferrea has a good antioxidant activity, probably due to dose of paracetamol in the samples investigated. However, more studies are needed for a better understanding of the effects of this extract compared to the effects found in this research
Objetivos: foi avaliado o efeito antioxidante do extrato etanólico da casca de Caesalpinia ferrea em modelo de estresse oxidativo induzido por paracetamol (acetaminofeno, PCM). Métodos: camundongos Swiss machos foram subdivididos em quatro grupos (controle; PCM; PCM+extrato; extrato; n=8) nos quais foi administrada uma dose de paracetamol (250 mg.kg-1) e após três horas foi administrado o tratamento com o extrato (100 mg.kg-1/ dia) por sete dias, via gavagem. Foram determinados biomarcadores de estresse oxidativo, como catalase, glutationa-S-transferase, glutationa reduzida, ácido ascórbico, substâncias reativas ao ácido tiobarbitúrico e proteínas carboniladas do fígado, rins e cérebro, além de parâmetros plasmáticos através da dosagem de glicose, colesterol, triglicerídeos, aspartato aminotransferase e alanina aminotransferase. Resultados: o extrato de Caesalpinia ferrea foi capaz de reverter os danos lipídicos e proteicos causados pela droga no tecido hepático, e também causou o mesmo efeito nos tecidos renal e cerebral nas proteínas carboniladas. O extrato sozinho diminuiu a atividade da glutationa-S-transferase hepática e aumentou a da catalase e glutationa-S-transferase cerebral, além de diminuir a glicose e o colesterol, mas sem alterar os triglicerídeos. Conclusões: foi possível concluir que o extrato etanólico da casca de Caesalpinia ferrea apresenta uma boa atividade antioxidante, provavelmente devido à presença de taninos, tendo em vista os danos causados pela alta dose de paracetamol nas amostras investigadas. Entretanto, mais estudos são necessários para um melhor entendimento dos efeitos deste extrato frente aos efeitos encontrados nesta pesquisa
Subject(s)
Animals , Biochemistry , Oxidative Stress , Caesalpinia , Plant Extracts , AcetaminophenABSTRACT
Biochemistry primarily focuses on the non-metal chemical elements carbon, oxygen, nitrogen, hydrogen, sulfur, and phosphorus in the four groups of building blocks (sugars, lipids, amino acids, and nucleotides) and the corresponding macromolecules. However, at least 10 essential chemical elements of life are metals. This article discusses the consequences of such a bias, presents current knowledge that over 20 chemical elements are required for life, and makes a case for-and suggests benefits of-teaching elemental biology alongside molecular biology and biochemistry, and inorganic chemistry in addition to organic chemistry. A relatively new interdisciplinary field, metallomics, has the potential to be a platform for integration when added to glycomics, lipidomics, proteomics, and genomics. It would fill a major gap in contemporary education, be relevant for many areas of science, and facilitate the teaching of important principles of chemistry in the biological sciences, thus helping students to gain a broader understanding of life processes from the molecular to the systemic biology level.
Subject(s)
Biochemistry , Biological Science Disciplines , Humans , Metals/metabolism , Molecular Biology , Phosphorus , TeachingABSTRACT
Rosemary, oregano, pink savory, lemon balm, St. John's wort, and saffron are common herbs wildly grown and easily cultivated in many countries. All of them are rich in antioxidant compounds that exhibit several biological and health activities. They are commercialized as spices, traditional medicines, or raw materials for the production of essential oils. The whole herbs or the residues of their current use are potential sources for the recovery of natural antioxidant extracts. Finding effective and feasible extraction and purification methods is a major challenge for the industrial production of natural antioxidant extracts. In this respect, the present paper is an extensive literature review of the solvents and extraction methods that have been tested on these herbs. Green solvents and novel extraction methods that can be easily scaled up for industrial application are critically discussed.
Subject(s)
Antioxidants/isolation & purification , Antioxidants/pharmacology , Biochemistry/methods , Plants, Medicinal/chemistry , Antioxidants/chemistry , SolventsABSTRACT
Dr. Regina Brigelius-Flohé (PhD 1978) is recognized here as redox pioneer because she has published an article on redox biology, as first author, that has been cited >1000 times, plus four articles cited >500 times, and a total of 30 articles cited >100 times. She obtained her doctorate in biochemistry at the Institute of Biochemistry of the University of Münster, Germany. She held positions in both, academia (Münster, Munich, Düsseldorf, Hannover, and Potsdam, Germany) and industry (Aachen, Germany). Dr. Brigelius-Flohé is the pioneer who, as head of the department of biochemistry of micronutrients of the German Institute of Human Nutrition (DIfE; Potsdam-Rehbrücke, Germany), worked out the metabolism of tocopherols and tocotrienols ("Key Finding 1"). She was the first to sequence glutathione peroxidase 4 (GPx4) ("Key Finding 2"), and unraveled the role of selenium, in particular of GPxs, in inflammation and carcinogenesis ("Key Finding 3"). Her contributions, thus, focused on serious biomedical problems such as nutrition, inflammation, and carcinogenesis. She has been a member of the scientific advisory board of the German Society of Biochemistry and Molecular Biology for 6 years and was president of SFRR-Europe in 2005-2006. She edited several books and serves on the editorial board of journals in the fields of nutrition, free radicals, and redox regulation. Antioxid. Redox Signal. 35, 595-601.
Subject(s)
Selenium , Biochemistry/history , Humans , Oxidation-Reduction , Selenium/metabolismABSTRACT
The objective of this paper is to compare conventional, ultrasound, microwave, and French press methods for the extraction of antioxidant compounds from Decatropis bicolor in an aqueous medium. This plant is widely used in Mexican traditional medicine for breast cancer treatment. Despite that, there are few studies on D. bicolor. Two response surface designs were applied to establish the best conditions of the liberation of antioxidants from D. bicolor, which were determined by DPPH⢠and Ferric Reducing Antioxidant Power (FRAP) techniques. The total phenolic content was evaluated by the Folin-Ciocalteu method. The results showed that D. bicolor is a source of antioxidants (669-2128 mg ET/100 g and 553-1920 mg EFe2+/100 g, respectively) and phenolic compounds (2232-9929 mg EGA/100 g). Among the physical factors that were analyzed, the temperature was the determinant factor to liberate the compounds of interest by using low concentrations of the sample and short times of extraction. The French press was the most efficient method, obtaining values of antioxidant activity and phenolic compounds even higher than those reported by using extraction methods with solvents such as methanol.
Subject(s)
Antioxidants/isolation & purification , Biochemistry/methods , Rutaceae/chemistry , Water/chemistry , Antioxidants/analysis , Biphenyl Compounds/chemistry , Iron/chemistry , Microwaves , Phenols/analysis , Picrates/chemistry , UltrasonicsABSTRACT
Metabolic reprogramming is now regarded as a hallmark of cancer. This phenomenon was first observed at the level of cellular energetics, in the form of very high rates of lactic acid fermentation, not only in anoxia, but also in the presence of oxygen levels that do not compromise respiration. This intriguing tumor phenotype, characterized by a very low energy yield, was unveiled, in the early 1920s, by Otto Warburg, one of the greatest biochemists of all time. This manuscript outlines aspects of Warburg's personal and research life that, in retrospect, might be viewed as a preparation for his successful approach to the cancer problem. It also discusses the experiments that led to the discovery and briefly presents Warburg's theory for the origin of tumors. Finally, it concludes with considerations regarding the novel avenues that this monumental and still intriguing discovery opened in terms of diagnosis and treatment of cancer.
Subject(s)
Cellular Reprogramming , Energy Metabolism , Neoplasms/metabolism , Biochemistry/history , History, 20th Century , History, 21st Century , Lactic Acid/metabolismABSTRACT
Colleges and educational institutions throughout the globe have adapted a pseudo-online educational model. This is also evident in health sciences courses designed for biochemical and chemical education. Biochemistry (along with general, organic and analytical) education is significant in the basic prerequisite education of undergraduate health sciences and non-majors curriculum, including a selection of human physiology and nutrition courses. Providing practical and empirical experiences for students enrolled in chemistry for health professions course is essential, even in this time of uncertainty. Here an option for a simple and safe, comprehensive virtual learning experience on carbohydrates and chemistry topics in connection with healthcare was developed by complementing an existing virtual lab collection and implementing this approach in an undergraduate health sciences (non-majors) course. Student responses to this online learning approach were recorded in a post-lab survey.
Subject(s)
Biochemistry/education , Carbohydrates , Computers , Curriculum , Education, Distance , Laboratories , Pandemics , Humans , Models, EducationalABSTRACT
Laboratórios clínicos desempenham um papel importante no apoio ao sistema do Serviço Nacional de Saúde, proporcionando saúde de qualidade para a população moçambicana. Como parte deste sistema, os laboratórios trabalham lado a lado com outros sectores para garantir testagem apropriada para o diagnóstico e acompanhamento (monitoria) de várias doenças. A Estratégia Nacional dos Laboratórios Clínicos de Moçambique (ENLC) foi desenvolvida em complementaridade do Plano Estratégico do Sector de Saúde 2014-2019, liderando esforços para fortalecer a rede de laboratórios clínicos em Moçambique e servirá para orientar, harmonizar e coordenar todas acções nacionais e internacionais ao longo dos próximos cinco anos. Sendo os laboratórios clínicos uma área transversal, os Objectivos Estratégicos, Metas, Indicadores e Actividades da ENLC foram desenvolvidos após uma revisão dos planos estratégicos anteriores, políticas e directrizes existentes, avaliações situacionais com envolvimento das partes interessadas e uma reflexão crítica sobre a crescente dependência de serviços laboratoriais de alta qualidade em todo o sistema de saúde. Reconhecendo a complexidade dos serviços laboratoriais de Moçambique, a disponibilidade de financiamento pode afectar o alcance dos Objectivos. Com efeito, solicita-se a todos os intervenientes que utilizam serviços de laboratório para apoiarem na disponibilização de recursos para fortalecer os serviços de diagnóstico laboratorial de qualidade, acessíveis à população moçambicana, com vista a se alcançar progressivamente a Cobertura Universalde Saúde, permitindo deste modo, a todos os moçambicanos, especialmente os mais vulneráveis, desfrutarem da melhor saúde possível. Espera-se também que as propostas de intervenções desta Estratégia sejam incorporadas nos planos dos gestores e implementadores do Sistema Nacional de Saúde de acordo com as responsabilidades que lhe forem conferidas e que sirva de inspiração usando-o no seu dia-a-dia.
Subject(s)
Humans , Population , Viral Load , Clinical Laboratory Techniques , Diagnostic Services , National Health Systems , Clinical Laboratory Services , Flow Cytometry , Hematology , Laboratories , Microbiology , Parasitology , Serology , Biochemistry , National Health ProgramsABSTRACT
MAIN CONCLUSION: Seed-processing technologies such as polishing and washing enhance crop seed quality by limited removal of the outer layers and by leaching. Combined, this removes chemical compounds that inhibit germination. Industrial processing to deliver high-quality commercial seed includes removing chemical inhibitors of germination, and is essential to produce fresh sprouts, achieve vigorous crop establishment, and high yield potential in the field. Sugar beet (Beta vulgaris subsp. vulgaris var. altissima Doell.), the main sugar source of the temperate agricultural zone, routinely undergoes several processing steps during seed production to improve germination performance and seedling growth. Germination assays and seedling phenotyping was carried out on unprocessed, and processed (polished and washed) sugar beet fruits. Pericarp-derived solutes, known to inhibit germination, were tested in germination assays and their osmolality and conductivity assessed (ions). Abscisic acid (ABA) and ABA metabolites were quantified in both the true seed and pericarp tissue using UPLC-ESI(+)-MS/MS. Physical changes in the pericarp structures were assessed using scanning electron microscopy (SEM). We found that polishing and washing of the sugar beet fruits both had a positive effect on germination performance and seedling phenotype, and when combined, this positive effect was stronger. The mechanical action of polishing removed the outer pericarp (fruit coat) tissue (parenchyma), leaving the inner tissue (sclerenchyma) unaltered, as revealed by SEM. Polishing as well as washing removed germination inhibitors from the pericarp, specifically, ABA, ABA metabolites, and ions. Understanding the biochemistry underpinning the effectiveness of these processing treatments is key to driving further innovations in commercial seed quality.
Subject(s)
Abscisic Acid/metabolism , Beta vulgaris/growth & development , Plant Growth Regulators/metabolism , Beta vulgaris/physiology , Biochemistry , Germination , Seeds/growth & development , Seeds/physiology , Tandem Mass SpectrometryABSTRACT
Laboratory exercises for undergraduate biochemistry students are described in which changes in sugar content during fermentation of the trendy beverage kombucha are analyzed by three methods: thin layer chromatography, a 3,5-dinitrosalicylic acid assay, and a standard commercial blood glucose meter. Each of the three analyses can be completed in a typical laboratory session lasting two to three hours. The exercises are designed to reinforce concepts typically covered in an undergraduate biochemistry course as well as to teach a variety of laboratory techniques. The exercises have been used with positive results in an upper level biochemistry laboratory course for junior/senior students majoring in chemistry or biology. © 2019 International Union of Biochemistry and Molecular Biology, 47(4):459-467, 2019.
Subject(s)
Biochemical Phenomena , Biochemistry/education , Education, Medical, Undergraduate , Laboratories , Tea/chemistry , Fermentation , Humans , Students , UniversitiesABSTRACT
La terapia de oxigenación hiperbárica (TOHB) es usada en el tratamiento de diferentes patologías y es prioritario conocer su injerencia en la bioquímica del paciente y establecer el perfil de seguridad. Con el objetivo de establecer la seguridad y conocer la bioquímica de seguimiento de pacientes que realizan el tratamiento, se realizó un estudio retrospectivo de individuos sanos evaluando el efecto de la TOHB (100% O2 a 1,4 atm) sobre algunos parámetros bioquímicos. Se estudió el estado basal, y a las 10 y 20 sesiones, de los valores de los indicadores de coagulación, hematológicos, función hepática y reactantes de fase aguda. En los 20 individuos sanos no hubo alteraciones en el examen clínico general debido a la exposición a la TOHB. La media (con DE) de los valores bioquímicos a las sesiones 0, 10 y 20 estuvieron siempre dentro del rango de valores de referencia. Estos resultados sugieren que el tratamiento en estas condiciones no produce cambios estadísticamente significativos en los parámetros estudiados.
Hyperbaric oxygenation therapy (HBOT) is used in the treatment of different pathologies and it is a priority to establish its safety profile. A retrospective study in healthy individuals was performed, evaluating the effect of HBOT at 1.4 ATA on biochemical indicators. The general and basal status and after 10 and 20 sessions of biochemical parameters in blood sample, on the biochemical markers of coagulation, hematologic values, hepatic function status, and acute reaction were analysed as well. For the 20 healthy subjects, there were no alterations in the general clinical examination due to exposure to HBOT. The mean values (with SD) of the biochemical parameters at 0, 10 and 20 sessions were mostly within reference values range. The results suggest that the treatment does not produce statistically significant changes in the biochemical parameters studied in these conditions.
A terapia de oxigenação hiperbárica (TOHB) é utilizada no tratamento de diferentes patologias e é prioritário conhecer sua ingerência na bioquímica do paciente e estabelecer o perfil de segurança. A fim de estabelecer segurança e conhecer a bioquímica de monitoramento de pacientes que realizam o tratamento, foi realizado um estudo retrospectivo de indivíduos saudáveis avaliando o efeito da OHB (100% O2 a 1,4 atm) em alguns parâmetros bioquímicos. O estado basal foi estudado e em 10 e 20 sessões os valores de coagulação, hematológicos, função hepática e reagentes de fase aguda foram estudados. Nos 20 indivíduos saudáveis, não houve alterações no exame clínico geral devido à exposição à TOHB. A média (com o DS) dos valores bioquímicos nas sessões 0, 10 e 20 estavam sempre dentro da faixa de valores de referência. Estes resultados sugerem que o tratamento sob estas condições não produz mudanças estatisticamente significativas ao nível dos parâmetros estudados.
Subject(s)
Therapeutics/methods , Biomarkers , Hyperbaric Oxygenation , Pathology , Reference Values , Temporomandibular Joint , Therapeutics , Biochemistry , Blood , Retrospective Studies , State , Healthy Volunteers , Indicators and ReagentsABSTRACT
Glucose oxidase (GOD) is an enzyme widely used in glucose monitoring systems owing to its high specificity towards glucose. However, in our previous work maltose was found to show significant interaction with GOD and based on this observation, a novel microplate-based method was developed to assess α-amylase inhibitory activity (GOD method). Concerns regarding the interaction of GOD with maltose has limited the widespread use of the GOD method in assessing α-amylase activity. The present paper provides answers to concerns regarding the interaction of GOD with maltose using HPLC studies and application of the GOD method in assessing α-amylase activity. According to the results, the newly developed GOD method can be considered as a well-suited method for the determination of α-amylase activity and as an easy method to do kinetic studies compared to other available methods.
Subject(s)
Biochemistry/methods , Glucose Oxidase/metabolism , Plant Extracts/pharmacology , alpha-Amylases/metabolism , Chromatography, High Pressure Liquid , Enzyme Inhibitors/pharmacology , Flour , Glucose/metabolism , Hydrolysis , Kinetics , Maltose/metabolism , Reproducibility of Results , Sensitivity and Specificity , Starch/metabolism , alpha-Amylases/antagonists & inhibitorsABSTRACT
Phosphorus is a major biogeochemical element controlling growth in many ecosystems. It has presumably been an important element since the onset of life. In most chemical and biochemical considerations, phosphorus is synonymous with phosphates, a pentavalent oxidation state that includes the phosphate backbone of DNA and RNA, as well as major metabolites such as ATP. However, redox processing of phosphates to phosphites and phosphonates, and to even lower oxidation states provides a work-around to many of the problems of prebiotic chemistry, including phosphorus's low solubility and poor reactivity. In addition, modern phosphorus cycling has increasingly identified reduced P compounds as playing a role, sometimes significant, in biogeochemical processes. This suggests that phosphorus is not redox-insensitive and reduced P compounds should be considered as part of the phosphorus biogeochemical cycle.
Subject(s)
Phosphorus/metabolism , Adenosine Triphosphate/metabolism , Biochemistry , Origin of Life , Oxidation-ReductionABSTRACT
Sporopollenin is a ubiquitous and extremely chemically inert biopolymer that constitutes the outer wall of all land-plant spores and pollen grains1. Sporopollenin protects the vulnerable plant gametes against a wide range of environmental assaults, and is considered a prerequisite for the migration of early plants onto land2. Despite its importance, the chemical structure of plant sporopollenin has remained elusive1. Using a newly developed thioacidolysis degradative method together with state-of-the-art solid-state NMR techniques, we determined the detailed molecular structure of pine sporopollenin. We show that pine sporopollenin is primarily composed of aliphatic-polyketide-derived polyvinyl alcohol units and 7-O-p-coumaroylated C16 aliphatic units, crosslinked through a distinctive dioxane moiety featuring an acetal. Naringenin was also identified as a minor component of pine sporopollenin. This discovery answers the long-standing question about the chemical make-up of plant sporopollenin, laying the foundation for future investigations of sporopollenin biosynthesis and for the design of new biomimetic polymers with desirable inert properties.
Subject(s)
Biopolymers/chemistry , Carotenoids/chemistry , Magnetic Resonance Spectroscopy/methods , Pinus/chemistry , Biochemistry/methods , Biopolymers/isolation & purification , Carotenoids/isolation & purification , Chromatography, High Pressure Liquid , Flavanones/chemistry , Freeze Drying , Hydrolysis , Molecular Structure , Pollen/chemistry , Polyvinyl Alcohol/chemistryABSTRACT
Lactase is the enzyme responsible for the digestion of the disaccharide lactose, and deficiency in this enzyme causes the prevalent medical condition lactose intolerance. Management of lactose intolerance can be achieved through the administration of lactase supplements. Lactase is an appropriate platform for advanced enzymatic study because its medical application is a motivator for student learning. The following is an upper-level biochemistry laboratory sequence that integrates student inquiry and exposure to advanced laboratory techniques. Students investigate three different lactase supplements through experimentation that includes the Bradford assay, SDS-PAGE, continuous and discontinuous kinetic assays, and zymography. Upon completion of this project, students compile their results and conclusions in a scientifically formatted paper comparing supplement protein content and activity. This safe and inexpensive laboratory project enriches student understanding of key biochemical concepts while mirroring work performed in a realistic research setting. © 2018 International Union of Biochemistry and Molecular Biology, 46(6):652-662, 2018.
Subject(s)
Biochemistry/education , Dietary Supplements/analysis , Lactase/analysis , Lactose Intolerance/diet therapy , Lactose Intolerance/enzymology , Humans , Laboratories , Lactase/metabolism , Lactose/metabolism , StudentsABSTRACT
The objectives of this study were to evaluate the serum biochemical profile in Quarter Horse mares in their peripartum, and neonates on their birth date, and to compare the values obtained for male and female foals, as well as values obtained for foals and mares on the day of delivery. Forty one adult, pregnant mares and their respective offspring on the day of delivery were analyzed, totaling 82 animals. Two samples of blood were collected from the mares, seven days before and on the day of delivery. From the foals, blood collection was performed on the day of their birth about 12 h after colostrum ingestion. The samples were centrifuged and the serum stored at -20 ºC for analyses. Each sample was used to determine the serum concentrations proteins, metabolites, minerals and enzymes. Physiological changes caused by peripartum led to higher serum total protein, albumin, and globulins on the day of delivery. Serum concentration of triglycerides and calcium /phosphorus ratio were greater seven days before delivery; iron concentration was higher than reference ranges in both moments of evaluation. In foals, albumin serum concentrations, albumin/globulins ratio, and aspartate aminotransferase remained below reference ranges. In foals, there was no influence of sex in the serum biochemical profile. When results obtained for foals on their birth date were compared with those obtained for the mares on the delivery day, cholesterol, triglycerides, phosphorus, iron, alkaline phosphatase, and gamma glutamyltransferase were higher in foals, and total protein, albumin, globulins, urea nitrogen, calcium/phosphorus ratio, and aspartate aminotransferase were higher in mares. Results showed variations in serum biochemistry in peripartum mares and neonates, as well as in the comparison between foals and mares on the day of delivery. Clinically, serum biochemistry values for peripartum mares and neonates should be considered in the analysis of laboratory results of tests carried out in this period.
Os objetivos deste estudo foram avaliar o perfil bioquímico sérico de éguas no periparto e potros no dia do nascimento, e confrontar os valores obtidos para potros machos e fêmeas, e desses com as éguas no dia do parto. Foram utilizadas 41 éguas adultas, gestantes, e suas respectivas crias no dia do nascimento, totalizando 82 animais. Das éguas foram coletadas duas amostras de sangue, sete dias antes do parto e no dia do parto. Dos potros, no dia do nascimento, aproximadamente 12 h após ingestão do colostro. As amostras foram centrifugadas e o soro estocado à 20º C para análises. De cada amostra foram determinadas as concentrações séricas de proteínas, metabólitos, minerais e enzimas. Alterações fisiológicas decorrentes do periparto determinaram um quadro com maiores valores sérico das proteínas totais, albumina e globulinas no dia do parto. A concentração sérica dos triglicérides e a relação cálcio/fósforo foi maior aos sete dias antes do parto e, o ferro com valores superiores aos de referência, nos dois momentos avaliados. Nos potros as concentrações séricas da albumina, relação albumina/globulinas e aspartato aminotransferase permaneceram abaixo dos valores de referência. Não houve influencia do sexo nos valores dos constituintes bioquímicos séricos avaliados. Quando confrontados os resultados obtidos para potros, no dia do nascimento, com os das éguas no dia do parto, observou-se maiores valores do colesterol, triglicérides, fósforo, ferro, fosfatase alcalina e gama glutamiltransferase nos potros, e nas éguas maiores valores para proteínas totais, albumina, globulinas, nitrogênio ureico, relação cálcio/fósforo e aspartato aminotransferase. Os resultados revelaram variações nos valores dos constituintes bioquímicos séricos nas éguas no peripato, nos potros neonatos e dos potros em relação as éguas no dia do parto. Clinicamente, os valores dos constituintes bioquímicos séricos nas éguas no periparto e potros neonatos, devem ser considerados no momento da interpretação de resultados de análises laboratoriais nesses períodos.
Subject(s)
Colostrum , Parturition , Peripartum Period , Horses , Biochemistry , Biomarkers , SerumABSTRACT
Resumen El síndrome de Leigh (SL) es una enfermedad neurodegenerativa, descrita como una encefalomielopatía necrotizante subaguda, y es una de las enfermedades de origen mitocondrial más frecuentes. El SL es causado por el déficit en la producción de energía, originada en defectos en los genes que codifican alguno de los complejos mitocondriales; el gen afectado puede ser de codificación tanto nuclear como mitocondrial, lo que explica que se encuentren diferentes mecanismos de herencia, incluyendo autosómica recesiva y herencia materna, lo que, a su vez, hace más difícil su diagnóstico molecular. Clínicamente se presenta con regresión del desarrollo cognitivo y pérdida de habilidades motoras con trastorno de movimiento, de rápida progresión. El diagnóstico se basa en la demostración bioquímica de la elevación del ácido láctico y de la relación lactato/piruvato, así como hallazgos en las neuroimágenes por resonancia magnética que muestran lesiones focales, bilaterales y simétricas en ganglios basales o tallo cerebral asociadas a leucoencefalopatía y atrofia cerebral. Se reportan cinco casos con diagnóstico clínico y bioquímico del SL que ejemplifican la variabilidad clínica y gravedad encontrada en este grupo de pacientes.
Summary Leigh syndrome (LS) is a neurodegenerative disease, described as a subacute necrotizing encephalomyelopathy and is one of the most frequent diseases of mitochondrial origin. LS is caused by a deficit in the energy production due to defects in the genes that encode some of the mitochondrial complexes. The affected gene can be due to either nuclear and/or mitochondrial coding, which explains why there are different ways of inheriting the disease, including autosomal recessive and maternal inheritance, which makes its molecular diagnosis even more difficult. Clinically, LS is characterized by regression in cognitive development and motor abilities, as well as movement disorders of rapid progression. Its diagnosis is based on the biochemical demonstration of an increase in lactic acid and lactate / pyruvate ratio, as well as magnetic resonance neuroimaging findings showing focal, bilateral and symmetric lesions in basal ganglia or brainstem associated with leukoencephalopathy and cerebral atrophy. Five cases are reported with clinical and biochemical diagnosis of LS that exemplify the clinical variability and severity found in this group of patients.
Resumo A síndrome de Leigh (SL) é uma doença neurodegenerativa, descrita como uma encefalomielopatia necrotizante subaguda e é uma das doenças de origem mitocondrial mais frequente. A SL é causada pelo déficit na produção de energia originada em defeitos nos genes que codificam algum dos complexos mitocondriais; o gene afetado pode ser de codificação tanto nuclear como mitocondrial, o que explica que se encontrem diferentes mecanismos de herança, incluindo autossômica recessiva e herança materna, o que torna mais difícil seu diagnóstico molecular. Clinicamente se apresenta com regressão do desenvolvimento do desenvolvimento cognitivo e perda de habilidades motoras com transtorno de movimento, de rápida progressão. O diagnóstico se baseia na demonstração bioquímica da elevação do ácido láctico e da relação lactato/piruvato, assim como descobertas nas neuro imagens por ressonância magnética que mostram lesões focais, bilaterais e simétricas em gânglios basais ou talo cerebral associadas a leucoencefalopatia e atrofia cerebral. Reportam-se cinco casos com diagnóstico clínico e bioquímico da SL que exemplificam a variabilidade clínica e gravidade encontrada neste grupo de pacientes.
Subject(s)
Humans , Leigh Disease , Biochemistry , Clinical Diagnosis , ColombiaABSTRACT
Avaliou-se a resposta dos parâmetros fisiológicos de frequência cardíaca (FC) e respiratória (FR), temperatura retal (TR) e a atividade sérica das enzimas creatina quinase (CK) e lactato desidrogenase (LDH) em cães adultos hígidos, machos e fêmeas, sob duas modalidades de hidroterapia. Dez cães foram distribuídos em dois grupos de cinco animais, denominados de grupo I (caminhada em esteira aquática) e grupo II (natação) e submetidos a duas sessões por semana com duração inicial de 10 minutos, acrescida de cinco minutos por sessão até 30 minutos, durante nove semanas. As avaliações foram realizadas antes, imediatamente depois e quatro horas após o exercício em todas as sessões para as variáveis de FC, FR e TR e nos dias um, sete, 28 e 60 para CK e LDH. Não houve diferença (P>0,05) quando comparados os valores médios dos parâmetros fisiológicos de frequência cardíaca e respiratória e de temperatura retal. Os níveis de CK e LDH não se elevaram após a hidroterapia nos diferentes tempos e dias avaliados. Dessa forma, foi possível concluir que as sessões de natação e de caminhada em esteira aquática não causaram alterações nos parâmetros fisiológicos de frequência cardíaca e respiratória, temperatura retal e nos níveis sanguíneos de creatina quinase (CK) e lactato desidrogenase (LDH) no protocolo proposto.(AU)
The physiological parameters of heart rate (HR), respiratory rate (RR), rectal temperature (TR), and serum activity of creatine kinase (CK) and lactate dehydrogenase (LDH) enzymes were determined in healthy dogs, male and female, under two hydrotherapy modalities. Ten dogs were distributed in two groups of five animals, named Group I (underwater treadmill walk) and Group II (swimming), submitted to two sessions per week with initial duration of 10 minutes, plus 5 minutes per session up to 30 minutes, during 9 weeks. The evaluations were performed before, immediately after and 4 hours after exercise in all sessions for the HR, RR and TR variables and on days one, seven, 28 and 60 for CK and LDH. There was no difference (P> 0.05) when comparing the mean values of physiological parameters of heart and respiratory rate and rectal temperature. CK and LDH levels did not rise after hydrotherapy at different times and days evaluated. So, it was possible to conclude that swimming sessions and underwater treadmill walking did not cause alterations in the physiological parameters of heart and respiratory rate, rectal temperature, and the blood levels of creatine kinase (CK) and lactate dehydrogenase (LDH) in the proposed protocol.(AU)