ABSTRACT
This study aimed to investigate the impact of incorporating kefir into the diet on biometric parameters, as well as the immune and antioxidant responses of the carpet shell clam (Ruditapes decussatus) after an experimental infection by Vibrio alginolyticus. Clams were divided into a control group and a treated group. The control group was fed on spirulina (Arthrospira platensis) alone. While, the treated group was fed on spirulina supplemented with 10% dried kefir. After 21 days, clams were immersed in a suspension of V. alginolyticus 5 × 105 CFU mL -1 for 30 min. Seven days after experimental infection, survival was 100% in both groups. The obtained results showed a slight increase in weight and condition index in clams fed with kefir-supplemented diet for 21 days compared to control clams. Regarding antioxidant responses, the treated group showed higher superoxide dismutase activity compared to the control group. However, the malondialdehyde level was lower in the treated clams than in the control. In terms of immune parameters, the treated group showed slightly elevated activities of phenoloxidase, lysozyme and alkaline phosphatase, whereas a decreased lectin activity was observed compared to the control group. The obtained results suggest that kefir enhanced both the antioxidant and immune response of infected clams.
Subject(s)
Adjuvants, Immunologic , Antioxidants , Bivalvia , Kefir , Probiotics , Superoxide Dismutase , Vibrio alginolyticus , Animals , Probiotics/pharmacology , Bivalvia/chemistry , Bivalvia/microbiology , Antioxidants/metabolism , Kefir/microbiology , Superoxide Dismutase/metabolism , Spirulina/chemistry , Malondialdehyde/metabolism , Malondialdehyde/analysis , Animal Feed , Monophenol Monooxygenase/metabolism , Dietary Supplements , Alkaline Phosphatase/metabolism , Muramidase/metabolism , Vibrio Infections/prevention & controlABSTRACT
BACKGROUND AND OBJECTIVE: Foods for Special Medical Purposes (FSMPs) are formulated to support the nutritional needs of subjects with impaired capacity to ingest, digest or absorb ordinary food or nutrients. Polglumyt® is a proprietary highly purified, high quality glycogen obtained from mussels. Here we report the results of a single-center, single dose, open label, single arm study carried out to investigate acceptance (i.e. gastrointestinal tolerance and palatability), metabolic profile and safety of a low osmolarity, high-density energy Polglumyt®-based drink (the investigational product, IP) as a novel FSMP. METHODS: Twelve healthy subjects received a single oral administration of the IP under fasting conditions. The study endpoints were: changes in gastrointestinal system tolerability at 3 h, 6 h and 24 h after IP intake; IP palatability evaluation; metabolic evaluation through the kinetic profile of circulating glucose, insulin and C-peptide from 0 h to 6 h after IP intake and changes from baseline in circulating triglycerides at 3 h and 6 h after IP intake. RESULTS: The IP showed a good gastrointestinal tolerability and an acceptable palatability. The IP did not affect the physiological glycemic profile and the triglycerides levels 6 h after the intake. The IP was well tolerated by study subjects, with no or minor adverse events. CONCLUSIONS: The study results encourage additional clinical investigations on the IP as a novel FSMP in patients with impaired digestion or gastrointestinal absorption, unable to assume an ordinary diet, e.g. patients undergoing invasive gastrointestinal surgery, elderly or oncological patients, even with certain metabolic disorders.
Subject(s)
Beverages , Dietary Supplements , Energy Intake/drug effects , Gastrointestinal Tract/drug effects , Glycogen/administration & dosage , Administration, Oral , Adult , Animals , Bivalvia/chemistry , Blood Glucose/drug effects , C-Peptide/blood , Fasting , Female , Food, Formulated , Glycogen/chemistry , Healthy Volunteers , Humans , Insulin/blood , Male , Middle Aged , Osmolar Concentration , Triglycerides/blood , Young AdultABSTRACT
The effects of lecithin addition at different concentrations (0-2.0%) on the physicochemical and emulsifying properties of mussel water-soluble proteins (MWP) were investigated. In solution system, low lecithin concentration (0.5%-1.0%) induced the aggregation and increased turbidity of composite particles. Lecithin addition caused changes in secondary structure and induced partial unfolding of MWP. Hydrophobic interactions between MWP and lecithin may contribute to the exposure of chromophores and hydrophobic groups of MWP. The interfacial tension decreased with lecithin addition. However, at a high lecithin concentration (1.5%-2.0%), the degree of aggregation and state of unfolding alleviated due to competitive adsorption. In emulsion system, with the low concentration of lecithin addition (0.5%-1.0%), droplet size and surface charge of emulsion decreased. The emulsion activity index, emulsion stability index, percentage of adsorbed protein increased. Both creaming stability and viscoelastic properties improved. At an intermediate lecithin concentration (1.0%), the emulsion showed the highest physical stability, while further addition of lecithin caused a slight deterioration in emulsifying properties. Overall, these results indicated the possibility that the lecithin-MWP mixed emulsifiers can be used to obtain emulsions with desirable properties.
Subject(s)
Bivalvia/chemistry , Emulsifying Agents/chemistry , Emulsions/chemistry , Lecithins/chemistry , Proteins/chemistry , Water/chemistry , Adsorption , Animals , Emulsifying Agents/analysis , Emulsions/analysis , Hydrophobic and Hydrophilic Interactions , Nephelometry and Turbidimetry , Particle Size , Protein Conformation , Protein Unfolding , Proteins/isolation & purification , Rheology , Surface Tension , Surface-Active Agents/chemistry , Viscoelastic Substances/analysis , Viscoelastic Substances/chemistry , ViscosityABSTRACT
Contaminated sediments at a pulp mill and former chor-alkali effluent treatment facility in Nova Scotia, Canada will undergo remediation. However, baseline studies assessing contaminants in marine biota in the marine receiving environment are lacking. Historical qualitative and quantitative contaminant data in biota from Boat Harbour (a former tidal lagoon which was used to treat industrial effluent since 1967), and surrounding marine environment were reviewed to establish baseline pollution from industrial effluent and contaminated sediments. Elevated metal, dioxins and furan concentrations previously measured in marine biota needs updating to help inform pre-remediation monitoring. Selection of species, contaminants of concern and sampling locations were ad hoc and often inconsistent with environmental effects monitoring requirements under Canadian federal Pulp and Paper Effluent Regulations. These consolidated baseline data are required to determine historical impacts and to assist future monitoring during Boat Harbour sediment remediation to compare against.
Subject(s)
Aquatic Organisms/chemistry , Water Pollutants, Chemical/analysis , Animals , Biota , Bivalvia/chemistry , Dioxins/analysis , Environmental Monitoring , Estuaries , Geologic Sediments/analysis , Industry , Metals/analysis , Nephropidae/chemistry , Nova ScotiaABSTRACT
A rapid screening method is described for the determination of paralytic shellfish toxins (PST), in fresh marine vectors (bivalves and gastropods), at levels ranging from 0.05 to 5.0 mg STX-eq kg-1. PST are extracted from marine vector homogenates with acetic acid according to the Pre-COX-LC-FLD method. At the same time, the obtained extract is oxidised simultaneously in hydrogen peroxide and periodate oxidate to determine PST, non-N-hydroxylated and N-hydroxylated toxins, respectively. Then, they are analysed using a microplate fluorometer (Ex: 335 nm/Em: 405 nm). All the samples were compared with the liquid chromatography post-column oxidation method. Recoveries of PST added to fresh and processed marine vectors averaged 93.9% with a coefficient of variation of 6.1%. Both methods showed a good linear regression (r2 = 0.97). The method shows good intra- and inter-day precisions with a relative coefficient of variation of ≈ 3.8% and 5.7%, respectively. The limit of quantification of the rapid screening fluorescence method was ≈ 0.082 mg STX-eq kg-1, with ≤5% false positives. The established rapid screening fluorescence methods offer highly effective and verifiable pre-analyses of PST contamination in marine vectors and can be used for routine screening of the PST in seafood before formal identification by confirmatory methods (Pre-COX LC-FLD method, Lawrence method).
Subject(s)
Bivalvia/chemistry , Fluorescence , Food Contamination/analysis , Gastropoda/chemistry , Marine Toxins/analysis , Shellfish Poisoning , Animals , Chromatography, Liquid , Drug Evaluation, Preclinical , Molecular Conformation , Spectrometry, FluorescenceABSTRACT
Okadaic acid (OA) is one of the most prevalent and largely distributed bio-toxin in the world. Consumption of OA results in a series of digestive ailments such as nausea and diarrhea. This study demonstrates the preparation and functioning of an electrochemical microfluidic biochip for the detection of OA. The screen-printed carbon electrode (SPCE) was modified by phosphorene-gold nanocomposite onto which an aptamer specific to OA was immobilized. BP-Au nanocomposites were synthesized by an in-situ, one-step method without the use of a reducing agent. Potassium ferro-ferri cyanide was used as a redox pair to quantify signal strength. To improve reaction time, increase sensitivity and portability, a microfluidic platform was designed and developed. This device comprised of channels identified for specific purposes such as sample mixing and incubation. Overall, the integrated system consisted of a polydimethylsiloxane microfluidic chip housing an aptamer modified SPCE, as a single detection module for Okadaic acid. The nanomaterials and the microfluidic channels prepared were spectroscopically and electrochemically analyzed. Differential pulse voltammograms revealed a detection limit of 8 pM, while a linear range was found between 10â¯nM-250â¯nM. Selectivity studies were also performed with spiked mussel samples and other interfering species. This point-of-care device can be deployed to perform on-farm assays in fishing units.
Subject(s)
Aptamers, Nucleotide/chemistry , Gold/chemistry , Nanocomposites/chemistry , Okadaic Acid/analysis , Phosphorus/chemistry , Animals , Biosensing Techniques/instrumentation , Bivalvia/chemistry , Equipment Design , Food Analysis/instrumentation , Lab-On-A-Chip Devices , Shellfish/analysisABSTRACT
Freshwater clams (Corbicula fluminea) have long been used as a folk remedy in Chinese tradition. Their hot-water extract has been commercialized as a functional drink for liver protection. The objective of this study was to develop a product of the residual clam meat (FCR) and assess its functional compounds. The ethanol extract of FCR, designated FCRE, was identified to comprise phytosterols, polyunsaturated fatty acids (PUFAs) and carotenoids. FCRE significantly reduced lipid accumulation and cell death in HepG2 cells via decreased fatty acid synthase (FAS) activity and increased activities of carnitine palmitoyltransferase (CPT) and acyl-CoA oxidase (ACO), indicative of suppressed lipogenesis and increased ß-oxidation of fatty acids. In tilapia fed with high-fat diet (HFD), FCRE mitigated nonalcoholic steatohepatitis (NASH), which was evidenced by decreased levels of plasma aspartate transaminase (AST) and alanine transaminase (ALT), in addition to reduced total cholesterol and accumulation of triacylglycerols, particularly those of saturated and monounsaturated fatty acids. FCRE also suppressed stearoyl-CoA desaturase-1 (SCD-1) index, increased the PUFAs' n3/n6 ratio, and reduced prostaglandin E2 (PGE2) and inflammatory infiltrates in tilapia liver. Tilapia fed with HFD for 2 weeks displayed NASH symptoms, while mice took 10 weeks to display NASH symptoms. No previous study has been reported on the potential use of tilapia as an NASH model for pre-screening hepatoprotective-functional foods.
Subject(s)
Bivalvia/chemistry , Non-alcoholic Fatty Liver Disease/drug therapy , Protective Agents/administration & dosage , Acyl-CoA Oxidase/metabolism , Animals , Carnitine O-Palmitoyltransferase/metabolism , Cholesterol/metabolism , Fatty Acid Synthases/metabolism , Fatty Acids/metabolism , Humans , Liver/drug effects , Liver/metabolism , Male , Meat/analysis , Mice , Mice, Inbred C57BL , Non-alcoholic Fatty Liver Disease/enzymology , Non-alcoholic Fatty Liver Disease/metabolism , Protective Agents/chemistry , Protective Agents/isolation & purification , Triglycerides/metabolismABSTRACT
Bivalve black clam, Villorita cyprinoides is traditional seafood in Southwestern coast of Arabian Sea. In this study, bioactivity-guided purification of ethyl acetate:methanol extract of black clam was carried out. Two irregular meroterpenoids {tetrahydro-3-methoxy-5-((E)-8,12-dimethyloct-8-enyl)-pyran-2-one (1) and dihydro-5-(8-(9,12-dihydro-8-methyl-11-propyl-2H-pyran-8-yl)-ethyl)-furan-2(3H)-one (2)} and two hexahydro-isochromenyl-meroterpenoids (3-4) were identified. The structures were characterized by detailed spectroscopy and their antioxidant/anti-inflammatory potentials were evaluated. Compound 2 registered significantly greater 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability (IC50 <0.65â¯mg/mL) than other analogues (IC50 ≥0.70â¯mg/mL). Ferrous ion (Fe2+) chelating ability was higher for 1-2 (IC50 â¼0.84â¯mg/mL) than α-tocopherol (IC50 0.89â¯mg/mL). The 5-lipoxygenase inhibitory potential of furano-meroterpenoid analogue, 2 (IC50 0.76â¯mg/mL) was greater than other compounds (IC50 >0.90â¯mg/mL). An optimum hydrophobic-hydrophilic balance of furano-meroterpenoid and lesser steric bulk manifested towards its greater bioactivities compared to other compounds. These anti-inflammatory and antioxidant leads could be used as potential functional food components.
Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Antioxidants/pharmacology , Bivalvia/chemistry , Furans/chemistry , Pyrans/chemistry , Animals , Anti-Inflammatory Agents, Non-Steroidal/chemistry , Antioxidants/chemistry , Drug Evaluation, Preclinical , Functional Food , Furans/pharmacology , Inhibitory Concentration 50 , Lipoxygenase Inhibitors/chemistry , Lipoxygenase Inhibitors/pharmacology , Magnetic Resonance Spectroscopy , Molecular Structure , Pyrans/pharmacology , Spectroscopy, Fourier Transform InfraredABSTRACT
Pearl powder, an important raw material in cosmetics and Chinese patent medicines, is commonly uneven in quality and frequently adulterated with low-cost shell powder in the market. The aim of this study is to establish an adequate approach based on Tri-step infrared spectroscopy with enhancing resolution combined with chemometrics for qualitative identification of pearl powder originated from three different quality grades of pearls and quantitative prediction of the proportions of shell powder adulterated in pearl powder. Additionally, computer vision technology (E-eyes) can investigate the color difference among different pearl powders and make it traceable to the pearl quality trait-visual color categories. Though the different grades of pearl powder or adulterated pearl powder have almost identical IR spectra, SD-IR peak intensity at about 861cm-1 (v2 band) exhibited regular enhancement with the increasing quality grade of pearls, while the 1082cm-1 (v1 band), 712cm-1 and 699cm-1 (v4 band) were just the reverse. Contrastly, only the peak intensity at 862cm-1 was enhanced regularly with the increasing concentration of shell powder. Thus, the bands in the ranges of (1550-1350cm-1, 730-680cm-1) and (830-880cm-1, 690-725cm-1) could be exclusive ranges to discriminate three distinct pearl powders and identify adulteration, respectively. For massive sample analysis, a qualitative classification model and a quantitative prediction model based on IR spectra was established successfully by principal component analysis (PCA) and partial least squares (PLS), respectively. The developed method demonstrated great potential for pearl powder quality control and authenticity identification in a direct, holistic manner.
Subject(s)
Bivalvia/chemistry , Electronics , Animals , Cluster Analysis , Color , Least-Squares Analysis , Powders , Principal Component Analysis , Spectroscopy, Fourier Transform InfraredABSTRACT
We report the results of 212 radiocarbon determinations from the archaeological excavation of 70 shell mound deposits in the Wathayn region of Albatross Bay, Australia. This is an intensive study of a closely co-located group of mounds within a geographically restricted area in a wider region where many more shell mounds have been reported. Valves from the bivalve Tegillarca granosa (Linnaeus, 1758) were dated. The dates obtained are used to calculate rates of accumulation for the shell mound deposits. These demonstrate highly variable rates of accumulation both within and between mounds. We assess these results in relation to likely mechanisms of shell deposition and show that rates of deposition are affected by time-dependent processes both during the accumulation of shell deposits and during their subsequent deformation. This complicates the interpretation of the rates at which shell mound deposits appear to have accumulated. At Wathayn, there is little temporal or spatial consistency in the rates at which mounds accumulated. Comparisons between the Wathayn results and those obtained from shell deposits elsewhere, both in the wider Albatross Bay region and worldwide, suggest the need for caution when deriving behavioural inferences from shell mound deposition rates, and the need for more comprehensive sampling of individual mounds and groups of mounds.
Subject(s)
Animal Shells/chemistry , Archaeology , Bivalvia/chemistry , Animal Shells/anatomy & histology , Animals , Archaeology/methods , Australia , Bays , Bivalvia/anatomy & histology , Carbon Radioisotopes/analysis , Fossils/history , History, 18th Century , History, AncientABSTRACT
Nature employs sophisticated control of a structure's properties at multiple length scales to achieve its wet adhesion. However, the translation of such structures has very often been missing in biomimetic adhesives; in turn, their performance is significantly limited as compared to that of biological adhesion, e.g., from mussels. In this Perspective, we overview the major breakthroughs in this field, highlighting the recent advances that demonstrate that holistic multiscale translation is essential to biomimetic design. We argue that the multiscale coordination of numerous key elements in the natural adhesive system is essential to replicate the strong, instant, and durable wet adhesion of the marine sessile organism.
Subject(s)
Biomimetic Materials/chemistry , Bivalvia/chemistry , Adhesiveness , Animals , WettabilityABSTRACT
A new fast decomposition procedure for solid samples was developed. In this study, we investigated decomposition of samples by lithium borates fusion in combination with salicylic acid. The method described shortens the time required for the sample to be digested and loaded to a resin to up to 2 h, and it was especially suitable for alpha spectrometry measurements in emergency situations. Additionally, the method results in high radiochemical recoveries and when compared to other digestion methods (classical digestion utilizing mineral acids, microwave digestion, and lithium borates fusion in combination with polyethylene glycol (PEG)) gives comparable activity concentration values. The method used was applied to various reference materials with inorganic and organic matrices as well as widely varying amounts of uranium radioisotopes content. The results obtained were compared with reference and literature values and show that the proposed method can be successfully implemented on various types of samples.
Subject(s)
Borates/chemistry , Lithium Compounds/chemistry , Salicylic Acid/chemistry , Animals , Bivalvia/chemistry , Bivalvia/metabolism , Microwaves , Polyethylene Glycols/chemistry , Radiometry , Seaweed/chemistry , Seaweed/metabolism , Soil/chemistry , Spectrometry, X-Ray Emission , Uranium/chemistryABSTRACT
Our purpose was to evaluate the protective effect of three marine omega-3 sources, fish oil (FO), krill oil (KO), and green-lipped mussel (GLM) against cartilage degradation. Canine cartilage explants were stimulated with either 10 ng/mL interleukin-1ß (IL-1ß) or IL-1ß/oncostatin M (10 ng/mL each) and then treated with various concentrations of docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA; 3 and 30 µg/mL), FO, KO, or GLM (250, 500, and 1000 µg/mL) for 28 days. Gene expression was then investigated in primary canine chondrocytes. Our results showed that DHA and EPA as well as omega-3 sources could suppress matrix degradation in cytokine-induced cartilage explants by significantly reducing the increase of sulfated glycosaminoglycans (s-GAGs) and preserving uronic acid and hydroxyproline content (except GLM). These agents were not able to reduce IL-1ß-induced IL1B and TNFA expression but were able to down-regulate the expression of the catabolic genes MMP1, MMP3, and MMP13 and up-regulate the anabolic genes AGG and COL2A1; FO and KO were especially effective. Our findings indicated that FO and KO were superior to GLM for their protective effect against proteoglycan and collagen degradation. Hence, FO and KO could serve as promising sources of chondroprotective agents.
Subject(s)
Cartilage, Articular/drug effects , Fatty Acids, Omega-3/pharmacology , Fish Oils/pharmacology , Animals , Bivalvia/chemistry , Cartilage, Articular/growth & development , Docosahexaenoic Acids/chemistry , Docosahexaenoic Acids/pharmacology , Dogs , Eicosapentaenoic Acid/chemistry , Eicosapentaenoic Acid/pharmacology , Euphausiacea/chemistry , Fatty Acids, Omega-3/chemistry , Fish Oils/chemistry , Gene Expression Regulation/drug effects , Glycosaminoglycans/metabolism , Interleukin-1beta/metabolism , Plant Extracts/metabolismABSTRACT
Two new meroterpeno 2H-pyranoids were isolated from the EtOAc:MeOH extract of yellow-foot clam Paphia malabarica. The structures of these newly reported compounds were elucidated based on spectroscopic interpretations. This is the first report of biogenic 2H-pyrans bearing decadienyl and allyloxy-(isopentanyl)-cyclohexene skeletons from marine biota. The extended C18 sesquiterpenoid with prenylated irregular farnesene framework was characterised as 2-((E)-deca-1,8-dien-10-yl)-11,12-dihydro-13-propyl-2H-pyran (1). The compound 2, 1'-((10E)-10-(10-(pentan-4-yl)-cyclohex-4-enyl)-allyloxy)-tetrahydro-2',2'-dimethyl-2H-pyran represents the first example of naturally occurring C21 prenylated bisabolene-type meroterpenoid, whereas tetrahydro-2',2'-dimethyl-2H-pyran remains attached at C-2' position of rearranged bisabolene framework formed by allyloxy linkage. The antioxidant activities (DPPH/ABTS+) of 1 and 2 were comparable (IC50 < 1.0 mg/mL) with α-tocopherol. In addition, these compounds exhibited greater activity against cyclooxygenase-2 than COX-1, and the selectivity indices were significantly lesser (~1.1). No significant differences in anti-5-lipoxygenase activity of 1 and 2 (IC50 1.02-1.06 mg/mL) than ibuprofen (IC50 0.93 mg/mL) indicated the potential anti-inflammatory properties of title compounds.
Subject(s)
Antioxidants/pharmacology , Bivalvia/chemistry , Cyclooxygenase Inhibitors/pharmacology , Lipoxygenase Inhibitors/pharmacology , Plant Extracts/pharmacology , Pyrans/pharmacology , Animals , Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Antioxidants/chemistry , Benzothiazoles/chemistry , Biphenyl Compounds/chemistry , Cyclooxygenase 1/drug effects , Cyclooxygenase 2/drug effects , Cyclooxygenase 2 Inhibitors/pharmacology , Cyclooxygenase Inhibitors/chemistry , Humans , Lipoxygenase Inhibitors/chemistry , Picrates/chemistry , Plant Extracts/chemistry , Pyrans/chemistry , Substrate Specificity , Sulfonic Acids/chemistryABSTRACT
Tegillarca granosa Linnaeus, possesses various biological functions and has been used a Chinese traditional medicine more than one century, but there is no report about anti-hyperplasia of mammary gland (HMG) activity of drugs from T. granosa. In this study, we investigated the anti-HMG effect of protein extract named HSS from T. granosa. The HMG model of virgin female Sprague Dawley rats was prepared by injecting estrogen in the thigh muscle of the rats and progestogen consecutively. HMG rats were treated with either HSS or positive control drug by i.g. for 35 consecutive days. In order to evaluate anti-HMG activity of HSS, Changes of nipple height and diameter, serum sex hormones levels, organ indexes and pathologic changes of mammary gland were performed. Body weight, food intake, pathomorphology examination of organs (heart, liver, spleen, lung, kidney), hematological and biochemical analysis were performed to evaluate the toxicity of HSS. HSS could significantly reduce nipples height and diameter, increase P concentration of HMG rat serum, spleen and thymus index, decrease uterus index, and has therapeutic effect on rat HMG and no toxicity at 500mg/kg/day. The anti-HMG mechanism of HSS may be related to AP-2α and P53. HSS has protective and therapeutic effects on HMG rats, and may be a promising agent for treating hyperplasia of mammary glands.
Subject(s)
Bivalvia/chemistry , Cell Extracts/pharmacology , Hyperplasia/chemically induced , Hyperplasia/drug therapy , Mammary Glands, Animal/drug effects , Animals , Body Weight/drug effects , Estrogens/administration & dosage , Estrogens/blood , Estrogens/toxicity , Female , Mammary Glands, Animal/pathology , Progestins/administration & dosage , Progestins/blood , Progestins/toxicity , Random Allocation , Rats , Spleen/drug effects , Thymus Gland/drug effects , Uterus/drug effectsABSTRACT
Recent reports of the widespread occurrence of the neurotoxin ß-N-methylamino-L-alanine (BMAA) in cyanobacteria and particularly seafood have raised concerns for public health. LC-MS/MS is currently the analytical method of choice for BMAA determinations but incomplete separation of isomeric and isobaric compounds, matrix suppression and conjugated forms are plausible limitations. In this study, capillary electrophoresis (CE) coupled with MS/MS has been developed as an alternative method for the quantitative determination of free BMAA. Using a bare fused silica capillary, a phosphate buffer (250 mM, pH 3.0) and UV detection, it was possible to separate BMAA from four isomers, but the limit of detection (LOD) of 0.25 µg mL-1 proved insufficient for analysis of typical samples. Coupling the CE to a triple quadrupole MS was accomplished using a custom sheath-flow interface. The best separation was achieved with a 5 M formic acid in water/acetonitrile (9:1) background electrolyte. Strong acid hydrolysis of lyophilized samples was used to release BMAA from conjugated forms. Field-amplified stacking after injection was achieved by lowering sample ionic strength with a cation-exchange cleanup procedure. Quantitation was accomplished using isotope dilution with deuterium-labelled BMAA as internal standard. An LOD for BMAA in solution of 0.8 ng mL-1 was attained, which was equivalent to 16 ng g-1 dry mass in samples using the specified extraction procedure. This was comparable with LC-MS/MS methods. The method displayed excellent resolution of amino acid isomers and had no interference from matrix components. The presence of BMAA in cycad, mussel and lobster samples was confirmed by CE-MS/MS, but not in an in-house cyanobacterial reference material, with quantitative results agreeing with those from LC-MS/MS. Graphical Abstract CE-MS separation and detection of BMAA, its isomers and the internal standard BMAA-d3.
Subject(s)
Amino Acids, Diamino/analysis , Electrophoresis, Capillary/methods , Food Contamination/analysis , Neurotoxins/analysis , Shellfish/analysis , Tandem Mass Spectrometry/methods , Animals , Bivalvia/chemistry , Cyanobacteria/chemistry , Cyanobacteria Toxins , Electrophoresis, Capillary/instrumentation , Equipment Design , Limit of Detection , Nephropidae/chemistry , Tandem Mass Spectrometry/instrumentationABSTRACT
Primary bone cancer brings patients great sufferings. To deal with the bone defects resulted from cancer surgery, biomaterials with good bone-forming ability are necessary to repair bone defects. Meanwhile, in order to prevent possible tumor recurrence, it is essential that the remaining tumor cells around bone defects are completely killed. However, there are few biomaterials with the ability of both cancer therapy and bone regeneration until now. Here, we fabricated a 3D-printed bioceramic scaffold with a uniformly self-assembled Ca-P/polydopamine nanolayer surface. Taking advantage of biocompatibility, biodegradability and the excellent photothermal effect of polydopamine, the bifunctional scaffolds with mussel-inspired nanostructures could be used as a satisfactory and controllable photothermal agent, which effectively induced tumor cell death in vitro, and significantly inhibited tumor growth in mice. In addition, owing to the nanostructured surface, the prepared polydopamine-modified bioceramic scaffolds could support the attachment and proliferation of rabbit bone mesenchymal stem cells (rBMSCs), and significantly promoted the formation of new bone tissues in rabbit bone defects even under photothermal treatment. Therefore, the mussel-inspired nanostructures in 3D-printed bioceramic exhibited a remarkable capability for both cancer therapy and bone regeneration, offering a promising strategy to construct bifunctional biomaterials which could be widely used for therapy of tumor-induced tissue defects.
Subject(s)
Biomimetic Materials/chemical synthesis , Bivalvia/chemistry , Bone Neoplasms/therapy , Guided Tissue Regeneration/methods , Nanostructures/administration & dosage , Phototherapy/methods , Tissue Scaffolds , Animals , Biocompatible Materials/chemical synthesis , Bone Neoplasms/pathology , Cell Line, Tumor , Ceramics/chemistry , Femoral Fractures/pathology , Femoral Fractures/therapy , Humans , Nanostructures/chemistry , Printing, Three-Dimensional , RabbitsABSTRACT
This study reports on an intelligent composite hydrogel with both pH-dependent drug release in a cancer environment and heat generation based on NIR laser exposure, for the combined application of photothermal therapy (PTT) and multidrug chemotherapy. For the first time in the literature, Dopamine nanoparticle (DP) was incorporated as a highly effective photothermal agent as well as anticancer drug, bortezomib (BTZ) carrier inside a stimuli responsive pNIPAAm-co-pAAm hydrogel. When light is applied to the composite hydrogel, DP nanoparticle absorbs the light, which is dissipated locally as heat to impact cancer cells via hyperthermia. On the other hand, facile release of the anticancer drug BTZ from the surface of DP encapsulated hydrogel could be achieved due to the dissociation between catechol groups of DP and the boronic acid functionality of BTZ in typical acidic cancer environment. In order to increase the synergistic effect by dual drug delivery, Doxorubicin (DOXO) were also loaded to pNIPAAm-co-pAAm/DP-BTZ hydrogel and the effect of monotherapy as well as combined therapy were detailed by a complete characterization. Our results suggest that these mussel inspired nanocomposite with excellent heating property and controllable multidrug release can be considered as a potential material for cancer therapy.
Subject(s)
Antineoplastic Agents/administration & dosage , Bivalvia/chemistry , Drug Carriers/chemistry , Hydrogel, Polyethylene Glycol Dimethacrylate/chemistry , Hydrogen-Ion Concentration , Nanocomposites/chemistry , Tocopherols , Animals , Biocompatible Materials/chemistry , Cell Line, Tumor , Cell Survival , Delayed-Action Preparations , Drug Delivery Systems , Drug Liberation , Humans , Hyperthermia, Induced , Materials Testing , Mice , Nanocomposites/ultrastructure , Polymerization , Spectrum Analysis, Raman , Thermogravimetry , X-Ray DiffractionABSTRACT
Batch-type experiments were used to study competitive As(V) and P sorption on various soils and sorbent materials. The materials assayed were a forest soil, a vineyard soil, pyritic material, granitic material, coarsely and finely ground mussel shell, calcinated mussel shell ash, pine sawdust and slate processing fines. Competition between As(V) and P was pronounced in the case of both soils, granitic material, slate fines, both shells and pine sawdust, showing more affinity for P. Contrary, the pyritic material and mussel shell ash showed high and similar affinity for As(V) and P. These results could be useful to make a correct use of the soils and materials assayed when focusing on As and P removal in solid or liquid media, in circumstances where both pollutants may compete for sorption sites.
Subject(s)
Adsorption , Arsenic/chemistry , Phosphorus/chemistry , Soil Pollutants/chemistry , Soil/chemistry , Waste Products , Wood/chemistry , Agriculture , Animal Shells , Animals , Bivalvia/chemistry , Environmental Monitoring , Forests , SpainABSTRACT
The evaluation of the levels of major colon microbiota populations (lactobacilli, bifidobacteria, enterobacteria) was carried out in two 15-days experiments on Wistar rats, exposed to stress factor (electric shock) and fed with different diets with the addition of biologic active micronutrients [extract from the leaves of Serratula coronata L. and Enzymatic hydrolyzate of the mussels meat (EHMM)]. In the first experiment animals were fed with a common vivarium diet. In the experimental group the water extract from leaves of Serratula coronata L. as a phytoecdysteroid source (5 mg per 1 kg body weight) was added to water. In the second experiment rats received balanced semisynthetic diet. In the diet of the experimental group the part of the protein (casein) was replaced by the peptides from EHMM. During the experiment the animal body weight was measured. On the 14th day of the experiment the animals were subjected to stress stimulation [electrodermal stimulation on paws (electric current 0.4 mA for 8 seconds)]. On the last day of the experiment the animals were euthanized by decapitation and micro-ecological research of protective microbiota populations in the cecal contents was carried out. The relative body weight increase was recorded in both experiments. In the second experiment in animals receiving EHMM this index (68.2 ± 3.0%) was considerably higher than in the control group and in the experimental group receiving no EHMM (57.2 ± 4.0 and 59.7 ± 2.8% respectively). The results of the microecological study showed different effect of diets with biologically active micronutrients on the population levels of lactobacilli. In the experiment with common vivarium diet no significant changes of the levels of the studied colon microbiota populations had been recorded in the rats of control group compared with rats of experimental group, exposed to stress factor but received no extract from Serratula coronata L. The decrease of the levels of lactobacilli by the end of the experiment was observed in the experimental group of rats received water extract from the leaves of Serratula coronata L (content of lactobacilli 7.76 ± 0.17 lg CFU/g) compared to those in control group and experimental group of rats received no extract (8.4 ± 0.09 and 8.69 ± 0.07 lg CFU/g respectively). Feeding with the balanced semisynthetic diet with the addition of EHMM or without it had a positive effect on the levels of lactobacilli and their balance with the aerobic component of the Enterobacteriaceae. There was a trend toward increased levels of lactic acid bacteria in the experimental group received EHMM (9.16 ± 0.12 lg CFU/g) compared with the contents in the control group and in the experimental group exposed to stress factor without adding EHMM in the diet (8.74 ± 0.34 and 8.79 ± 0.23 lg CFU/g, respectively). The conclusion about the positive (protective) effect of a semisynthetic diet enriched with peptides from EHMM was made based on the comparison of indicators that reflect the status of non-specific resistance of the organism: the integral criterion of weight gain and the levels of major colon microbiota populations of laboratory animals.