ABSTRACT
Periosteal derived bovine osteoblast-like cells migrated in culture onto an ionomeric cement. Cell cultures were maintained for 4 weeks and used to study the in vitro behaviour of cells on the ionomeric bone cement (IC). The cells produced bone matrix proteins (osteocalcin, bone sialoprotein II) and were osteoblast-like. The osteoblast-like cells colonized the substrate in monolayers and produced an extracellular matrix as seen by light and scanning electron microscopy. Morphological comparison between cells growing on the ionomeric bone cement and cortical bone revealed no significant difference in phenotypic expression. Staining for aluminium in osteoblasts growing on the IC showed an uptake and storage of aluminium in the cells. Energy dispersive X-ray microanalysis revealed high concentrations of aluminium and silicon in the periosteal tissue. Despite the known toxic effect of aluminium in vivo and in vitro on osteoblasts, no signs of toxicity were apparent on light and scanning electron microscopy analysis.
Subject(s)
Biocompatible Materials/pharmacology , Bone Cements/pharmacology , Osteoblasts/drug effects , Aluminum Oxide/metabolism , Aluminum Oxide/toxicity , Animals , Biocompatible Materials/metabolism , Biocompatible Materials/toxicity , Bone Cements/metabolism , Bone Cements/toxicity , Cattle , Cell Differentiation/drug effects , Cell Division/drug effects , Cells, Cultured , Evaluation Studies as Topic , Microscopy, Electron, Scanning , Osteoblasts/cytology , Silicon/metabolism , Silicon/toxicity , Staining and Labeling/methodsABSTRACT
A-cyanoacrylate proves to be an advanced means of osteosynthesis. It is particularly indicated for short bone fragments in the articular and comminuted fractures, where a perfect anatomic reduction is required. It is shown to be non-toxic for bone and surrounding tissues and to be endowed with a remarkable chemical bond. It is easily applied and inexpensive. The results are very interesting and stimulate continuation of the studies. The authors think a-cyanoacrylate (or a derivative) is the future of biological osteosynthesis.
Subject(s)
Bone Cements/therapeutic use , Cyanoacrylates/therapeutic use , Femoral Fractures/surgery , Fracture Fixation, Internal/methods , Animals , Bone Cements/toxicity , Cyanoacrylates/toxicity , Drug Evaluation, Preclinical , Femoral Fractures/pathology , Femur/drug effects , Femur/pathology , RabbitsSubject(s)
Bone Cements/toxicity , Methylmethacrylates/toxicity , Animals , Bone and Bones/drug effects , Dogs , Drug Evaluation, Preclinical , Female , Male , Mice , RatsABSTRACT
Evidence for the lack of toxicity of various bioglass formulations has been deduced from studies carried out, both in vivo and in vitro, in several different centers. Recent studies of the authors, described here, include testing of solid bioglass implants in the soft tissues of rats and rabbits for time periods of up to eight weeks. Two new techniques are described for the toxicological testing of particulate biomaterials. These tests, which involve rat peritoneal macrophages in culture and a mouse pulmonary biomaterial embolus model, indicate the biocompatibility of bioglass powders. Thus, the surface activity so critical in bone adhesion is without toxic effect in non-osseous tissues in contact with solid bioglass implants. Should wear occur and produce particulate bioglass, the material should be eliminated without consequence.