ABSTRACT
Staphylococcus aureus is currently a significant multidrug-resistant bacterium, causing severe healthcare-associated and community-acquired infections worldwide. The current antibiotic regimen against this pathogen is becoming ineffective due to resistance, in addition, they disrupt the normal microbiota. It highlights the urgent need for a pathogen-specific drug with high antibacterial efficacy against S. aureus. α-Viniferin, a bioactive phytochemical compound, has been reported to have excellent anti-Staphylococcus efficacy as a topical agent. However, so far, there were no clinical trials that have been conducted to elucidate its efficacy. The present study aimed to investigate the antibacterial efficacy of α-viniferin against S. aureus in a ten-day clinical trial. Based on the results, α-viniferin showed 50% minimum inhibitory concentrations (MIC50 values) of 7.8 µg/ml in culture broth medium. α-Viniferin was administered in the nares three times a day for ten days using a sterile cotton swab stick. Nasal swab specimens were collected before (0 days) and after finishing the trial (10th day), and then analyzed. In the culture and RT-PCR-based analysis, S. ureus was reduced significantly: 0.01. In addition, 16S ribosomal RNA-based amplicon sequencing analysis showed that S. aureus reduced from 51.03% to 23.99% at the genus level. RNA-seq analysis was also done to gain insights into molecular mechanisms of α-viniferin against S. aureus, which revealed that some gene groups were reduced in 5-fold FC cutoff at two times MIC conditions. The study results demonstrate α-viniferin as a potential S. aureus-specific drug candidate.
Subject(s)
Anti-Bacterial Agents/pharmacology , Benzofurans/pharmacology , Microbiota/drug effects , Plant Extracts/pharmacology , Staphylococcal Infections/drug therapy , Staphylococcus aureus/drug effects , Adult , Bacteria/classification , Bacteria/drug effects , Bacteria/genetics , Bacteria/metabolism , Carex Plant/chemistry , Female , Humans , Male , Microbial Sensitivity Tests , Middle Aged , Skin/microbiology , Staphylococcal Infections/microbiology , Staphylococcus aureus/genetics , Staphylococcus aureus/growth & development , Young AdultABSTRACT
Naturally derived compounds represent a potential source of pharmacologically active drugs able to contrast different diseases, including gastric cancer, a multifactorial disease, in which the important role played by H. pylori infection has been demonstrated. Carexanes, stilbene derivatives, isolated from plants of the Carex distachya Desf., are unusual secondary metabolites with a tetracyclic skeleton arising from a cyclization of prenylstilbenoid precursors. In this study we firstly showed the ability of three purified carexanes CxB, CxG, and CxI to enhance the antioxidant response of AGS cells and to contrast the effect of the H. pylori's protein HspB. Among them CxI was the molecule that best modified the expression of genes involved in the antioxidant response. In particular, CxI was able to reduce Keap-1 gene expression and induce NQO1 gene expression, both at 4 and 24 h in AGS cells, as showed by real time PCR. Nrf2 induction was evident only at 24 h. Interestingly, the effect of CxI was stronger in HspB-transfected AGS cells, where Keap-1 gene expression was nearly abrogated. Finally, we demonstrated that CxI was able to reduce also COX-2 gene expression in HspB-transfected AGS cells, compared with untreated HspB-transfected cells, both at 4 and 24 h. This study first report that carexanes might represent candidate molecules able to contrast the deleterious effect of HspB protein but also to reduce the inflammatory process induced by H. pylori infection.
Subject(s)
Antioxidants/metabolism , Bacterial Proteins/metabolism , Heat-Shock Proteins/metabolism , Helicobacter Infections/genetics , Helicobacter pylori/pathogenicity , Stilbenes/pharmacology , Carex Plant/chemistry , Cell Culture Techniques , Cell Line , Cell Proliferation/drug effects , Cyclooxygenase 2/drug effects , Cyclooxygenase 2/genetics , Epithelial Cells , Gastric Mucosa/drug effects , Gene Expression , Humans , Kelch-Like ECH-Associated Protein 1/drug effects , Kelch-Like ECH-Associated Protein 1/genetics , NF-E2-Related Factor 2/metabolism , Plant Extracts/isolation & purification , Plant Extracts/pharmacology , Stilbenes/chemistry , Stomach Neoplasms/drug therapy , TransfectionABSTRACT
Two new phenolics, a stilbenoid, vulpinoideol A (1), and a chalcone, vulpinoideol B (2), along with ten known compounds (3-12) were isolated from Carex vulpinoidea Michx. seeds. The structures of compounds 1-12 were elucidated based on spectrometric and spectroscopic analyses including HRESIMS, 1D and 2D NMR data. All compounds were evaluated for their tyrosinase enzyme inhibitory activities.
Subject(s)
Carex Plant/chemistry , Monophenol Monooxygenase/antagonists & inhibitors , Phenols/chemistry , Phenols/pharmacology , Seeds/chemistry , Molecular StructureABSTRACT
Pathogenic biofilms are associated with persistent infection due to their high resistances to diverse antibiotics. Pseudomonas aeruginosa infects plants, animals, and humans and is a major cause of nosocomial diseases in patients with cystic fibrosis. In the present study, the antibiofilm abilities of 522 plant extracts against P. aeruginosa PA14 were examined. Three Carex plant extracts at a concentration of 200 µg/mL inhibited P. aeruginosa biofilm formation by >80% without affecting planktonic cell growth. In the most active extract of Carex pumila , resveratrol dimer ε-viniferin was one of the main antibiofilm compounds against P. aeruginosa. Interestingly, ε-viniferin at 10 µg/mL inhibited biofilm formation of enterohemorrhagic Escherichia coli O157:H7 by 98%. Although Carex extracts and trans-resveratrol are known to possess antimicrobial activity, this study is the first to report that C. pumila extract and ε-viniferin have antibiofilm activity against P. aeruginosa and E. coli O157:H7.
Subject(s)
Benzofurans/pharmacology , Carex Plant/chemistry , Escherichia coli O157/drug effects , Plants, Medicinal/chemistry , Pseudomonas aeruginosa/drug effects , Stilbenes/pharmacology , Anti-Bacterial Agents/pharmacology , Biofilms/drug effects , Biofilms/growth & development , Dose-Response Relationship, Drug , Drug Evaluation, Preclinical/methods , Escherichia coli O157/physiology , Plant Extracts/pharmacology , Pseudomonas aeruginosa/physiology , Resveratrol , Stilbenes/analysisABSTRACT
The use of plants as a source of medicine is an important component of the health care system in rural India. Carex baccans (Cyperaceae) and Potentilla fulgens (Rosaceae) have been known since ancient times in northeast India for their antitumor, antidiabetic, and antihelmintic properties. The present study was designed to determine the subacute toxicity profile of the root tuber extract of C. baccans and root-peel extract of P. fulgens in Wistar rats. The subacute oral toxicity was conducted using sublethal doses of 40, 50, 100, 150, 200, and 400 mgkg-1 body weights. Surface topographical and ultrastructural observations of liver and intestinal microvilli showed remarkable deformation and disruption, accompanied by quantitative changes in the liver enzymes, i.e., aspartate aminotransferase and alanine aminotransferase in comparison to those of the control group. Apoptotic cell death was observed in the liver cells of rats exposed to both of the plant extracts. A significant increase in splenic lymphocyte count was also observed in rats exposed to the highest concentration of both extracts. The results showed that consumption of the plant extracts at higher doses may cause toxicological effect if treatment continues for a long time.
Subject(s)
Carex Plant/chemistry , Liver/ultrastructure , Microvilli/ultrastructure , Plant Extracts/toxicity , Potentilla/chemistry , Alanine Transaminase/metabolism , Animals , Apoptosis/drug effects , Aspartate Aminotransferases/metabolism , Female , India , Liver/cytology , Liver/drug effects , Liver/enzymology , Male , Microvilli/drug effects , Rats , Rats, WistarABSTRACT
Two new clerodane diterpenes were isolated from roots of Carex distachya Desf., a perennial plant widely distributed in the coastal area of the Mediterranean basin. Chemical characterization of the metabolites was carried out mainly by 1D and 2D NMR spectroscopy. The isolated compounds influenced either positively or negatively the plant growth (root and shoot elongation) of three coexisting herbaceous species.
Subject(s)
Carex Plant/chemistry , Diterpenes, Clerodane/isolation & purification , Caryophyllaceae/drug effects , Diterpenes, Clerodane/chemistry , Diterpenes, Clerodane/pharmacology , Pheromones , Plant Roots/chemistry , Poaceae/drug effectsABSTRACT
Plants of the Carex genus (Family: Cyperaceae) have attracted recent attention as potential food additives because they contain high levels of bioactive polyphenols commonly found in plant foods. Seven compounds, which included two resveratrol oligomers and five flavonoids, were isolated from seeds of Carex folliculata L. (northern long sedge), a forage prevalent in the northern United States. The compounds were identified by (1)H and (13)C nuclear magnetic resonance and mass spectrometry data. The resveratrol oligomers were pallidol (1), a resveratrol dimer reported to be present in levels equivalent to those of resveratrol in red wine, and kobophenol A (2), a resveratrol tetramer with a unique 2,3,4,5-tetraaryltetrahydrofuran skeleton. The flavonoids were isoorientin (3), luteolin (4), quercetin (5), 3-O-methylquercetin (6), and rutin (7). Compounds were evaluated for antioxidant activity in the diphenylpicrylhydrazyl (DPPH) radical scavenging assay; cytotoxicity activity against human colon (HCT116, HT29) and breast (MCF7, MDA-MB-231) tumor cell lines; and antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA). The antioxidant activities of the flavonoids (3-7; IC(50) values ranging from 50 to 200 microM) were comparable to that of ascorbic acid (IC(50) = 60 microM) and superior to those of the resveratrol derivatives (1 and 2; IC(50) > 1000 microM) and butylated hydroxytoluene (BHT; IC(50) = 1500 microM), a commercial antioxidant. In the cytotoxicity and antibacterial bioassays, compounds 4 (IC(50) for HCT116 = 45 microM) and 6 (IC(50) for MRSA = 6.4 microM) were the most active, respectively. Therefore, given the wide availability and underutilization of C. folliculata, this forage may provide a source of bioactive compounds useful for nutraceutical purposes. Also, this is the first reported phytochemical investigation of C. folliculata.
Subject(s)
Carex Plant/chemistry , Flavonoids/chemistry , Flavonoids/pharmacology , Plant Extracts/chemistry , Plant Extracts/pharmacology , Stilbenes/chemistry , Stilbenes/pharmacology , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/isolation & purification , Anti-Bacterial Agents/pharmacology , Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/isolation & purification , Antineoplastic Agents, Phytogenic/pharmacology , Cell Line, Tumor , Cell Proliferation/drug effects , Flavonoids/isolation & purification , Humans , Methicillin-Resistant Staphylococcus aureus/drug effects , Neoplasms/physiopathology , Plant Extracts/isolation & purification , Resveratrol , Seeds/chemistry , Stilbenes/isolation & purificationABSTRACT
In the present study, the chemical composition and antioxidant properties of root methanol extract of Carex distachya Desf. (Cyperaceae) were assessed to use this plant as sources of food additives and nutraceuticals. The IC50 of the extract (4.2 microg/mL), derived from the DPPH radical scavenging capacity assay, was similar to those of ascorbic acid, alpha-tocopherol, and BHT. These results revealed a strong antioxidant activity because of the presence of an extraordinary quantity of bioactive phytochemicals. The phytochemical study of the root extract led to the isolation and identification of new and known polyphenols, most of them common constituents of plant foods. A total of 16 polyphenols, identified on the basis of spectroscopic data as 7 lignans, 4 phenylethanoids, 3 resveratrol derivatives, a monolignol, and a secoiridoid glucoside, were isolated. The tentative structural elucidation of the new metabolites 5'-O-beta-D-glucopyranosyloxy-3,3'-dimethoxy-7,9'-epoxylignan-4,8',9-triol and 3,5-bis-O-beta-D-glucopyranosyloxy-3'-methoxy-trans-stilben-4'-ol have been performed by a combined approach using ESI/TQ/MS techniques and 1D and 2D NMR experiments. All of the compounds have been tested for their antioxidant activity using six different antioxidant and radical scavenging tests. Interestingly, the extract contained high quantities of polyphenols, most of them reported as constituents of edible plants, such as grape and olive, suggesting that the methanol root extract of this plant could be used as a source of natural antioxidants useful as potential food additives.
Subject(s)
Antioxidants/pharmacology , Carex Plant/chemistry , Flavonoids/analysis , Food Additives/analysis , Phenols/analysis , Plant Roots/chemistry , Flavonoids/pharmacology , Free Radical Scavengers/pharmacology , Phenols/pharmacology , Plant Extracts/chemistry , PolyphenolsABSTRACT
Two new dibenzoxazepinones have been isolated from the leaves of Carex distachya, an herbaceous plant growing in the Mediterranean area. The structures have been elucidated on the basis of their spectroscopic properties. Bidimensional NMR (DQ-COSY, TOCSY, NOESY, ROESY, HSQC, and HMBC) furnished important data useful for the characterization of the molecules. The compounds have been assayed, for the antioxidant activity, by measuring its capacity to scavenge the DPPH, the superoxide anion, and nitric oxide radicals.
Subject(s)
Carex Plant/chemistry , Dibenzoxazepines/chemical synthesis , Dibenzoxazepines/pharmacology , Free Radical Scavengers/pharmacology , Biphenyl Compounds , Dibenzoxazepines/chemistry , Free Radical Scavengers/chemistry , Free Radical Scavengers/isolation & purification , Magnetic Resonance Spectroscopy , Nitric Oxide/chemistry , Nitroblue Tetrazolium , Nitroprusside/chemistry , Picrates , Plant Extracts/pharmacology , Plant Leaves/chemistry , Spectrometry, Mass, Electrospray Ionization , Spectrophotometry, Infrared , Spectrophotometry, Ultraviolet , Superoxides/chemistryABSTRACT
Four metabolites, named carexanes I-L, have been isolated from the roots of Carex distachya Desf, an herbaceous plant living in the Mediterranean maquis, together with three known compounds, already isolated from the aerial part of the plant. All the compounds have been characterized on the basis of their spectroscopic properties. Carexane I derived from the lose of a proton from the C-18 carbon of an intermediate isopropyl cation. Its stereostructure has been elucidated by Mosher's method, NOESY/ROESY experiments and computational calculations. The bioactivity on seed germination and root/shoot growth of Lactuca sativa L. of all the isolated compounds is also reported.