ABSTRACT
Objective: This study represents a viable assessment of the effect of the low-level laser (LLL) of 635 nm and ultraviolet (UV) of 265 nm on biophysical properties of blood. Materials and methods: Blood samples were divided into two main groups: one for irradiation by LLL and the other for irradiation by UV. Each group was divided into three aliquots. First aliquot: whole blood was exposed to radiation. The second aliquot: erythrocytes were exposed to radiation and resuspended in autologous plasma. The third aliquot: plasma was exposed to radiation, and erythrocytes were resuspended in it. The following parameters were measured after irradiation by LLL and UV for all aliquots: whole blood viscosity, microscopic aggregation index, deformation index, and Zeta potential. Results: A decrease in whole blood viscosity due to irradiation by LLL was observed. To the contrary, an increase in whole blood viscosity due to irradiation by UV was detected. A significant reduction in erythrocytes' aggregation was observed as a result of LLL and UV radiation. Erythrocytes' deformability was strongly affected by UV radiation, while there was no significant effect from LLL. Another noticeable change observed was an increase in Zeta potential due to UV and a decrease in Zeta potential values, as a result of LLL irradiation. Conclusions: It can be concluded from this study that LLL and UV can be used to change some biological processes, as well as cellular properties.
Subject(s)
Blood/radiation effects , Low-Level Light Therapy/methods , Adult , Blood Viscosity/radiation effects , Dose-Response Relationship, Radiation , Erythrocyte Aggregation/radiation effects , Erythrocyte Deformability/radiation effects , Erythrocytes/radiation effects , Healthy Volunteers , Humans , In Vitro Techniques , Male , Middle Aged , Plasma/radiation effects , Ultraviolet RaysABSTRACT
The aim of the present investigation is to study the influence of low-intensity laser therapy at the aggregate function of erythrocytes in patients with chronic pancreatitis. 118 patients aged 36-77 years old were examined and divided into 2 groups: the experimental (73 patients) and the control group (45 patients). The experimental group has had a complex drug and laser therapy of various therapeutic techniques. Control group has had only drug therapy. In this study were found out various changes of aggregate properties of erythrocytes in patients with exacerbation of chronic pancreatitis. Was established normalizing effects of low-intensity laser therapy on erythrocytes aggregation in experimental group.
Subject(s)
Erythrocyte Aggregation/drug effects , Erythrocyte Aggregation/radiation effects , Low-Level Light Therapy , Pancreatitis, Chronic/blood , Pancreatitis, Chronic/therapy , Adult , Aged , Female , Humans , Male , Middle AgedABSTRACT
Brain lateral light-colour stimulation-induced changes in bulbar microcirculation were studied in 47 patients (29 male and 18 female subjects) with chronic glomerulonephritis. After stimulation, a drop in arterial pressure was noted together with a decrease in red cell aggregation, dilation of arteries, increase in the number of functioning capillaries and in the velocity of microcirculation. A conclusion has been reached that the above stimulations can be an effective method for improvement of microcirculation in patients with chronic glomerulonephritis.
Subject(s)
Glomerulonephritis/therapy , Pyramidal Tracts/blood supply , Adolescent , Adult , Arterioles/pathology , Arterioles/radiation effects , Blood Pressure/radiation effects , Capillaries/pathology , Capillaries/radiation effects , Chronic Disease , Color Therapy , Erythrocyte Aggregation/radiation effects , Glomerulonephritis/blood , Glomerulonephritis/complications , Glomerulonephritis/physiopathology , Humans , Hypertension/complications , Male , Microcirculation , Middle Aged , Phototherapy , Regional Blood FlowABSTRACT
Properties of erythrocyte surface were investigated for patients with ischemic heart disease in the course of treatment with the UV-irradiated blood autotransfusion (UVIBA). Application of methods of light-scattering, photometry and cytochemistry revealed rapid and significant changes in deformability and aggregation properties of the erythrocytes immediately following each UVIBA procedure, which was accompanied by considerable blood viscosity decrease.