Comparative In Vitro Study: Examining 635 nm Laser and 265 nm Ultraviolet Interaction with Blood.

Objective: This study represents a viable assessment of the effect of the low-level laser (LLL) of 635 nm and ultraviolet (UV) of 265 nm on biophysical properties of blood. Materials and methods: Blood samples were divided into two main groups: one for irradiation by LLL and the other for irradiation by UV. Each group was divided into three aliquots. First aliquot: whole blood was exposed to radiation. The second aliquot: erythrocytes were exposed to radiation and resuspended in autologous plasma. The third aliquot: plasma was exposed to radiation, and erythrocytes were resuspended in it. The following parameters were measured after irradiation by LLL and UV for all aliquots: whole blood viscosity, microscopic aggregation index, deformation index, and Zeta potential. Results: A decrease in whole blood viscosity due to irradiation by LLL was observed. To the contrary, an increase in whole blood viscosity due to irradiation by UV was detected. A significant reduction in erythrocytes' aggregation was observed as a result of LLL and UV radiation. Erythrocytes' deformability was strongly affected by UV radiation, while there was no significant effect from LLL. Another noticeable change observed was an increase in Zeta potential due to UV and a decrease in Zeta potential values, as a result of LLL irradiation. Conclusions: It can be concluded from this study that LLL and UV can be used to change some biological processes, as well as cellular properties.

Effect of low power laser irradiation on disconnecting the membrane-attached hemoglobin from erythrocyte membrane.

In our previous study we found that low power laser irradiation improved the erythrocyte deformability, but the mechanism is unclear. The membrane-attached hemoglobin (Hbm) may be one of the determining factors for the erythrocyte deformability. We report here for the first time, that laser irradiation can reduce the Hbm contents in pig's erythrocytes, providing the explanation for the improvement of erythrocyte deformability. The decrease of the Hbm was proportional to the irradiation dose, but the relative change of Hbm was saturated around 35%. The 532 nm laser was more efficient at lowering Hbm than the 632.8 nm laser, consistent with the absorption spectrum of Hbm.

[The effect of quantum hemotherapy on cellular and humoral immunity and on erythrocyte morphology in patients with ischemic heart disease]. / Vliianie kvantovoi gemoterapii na sostoianie kletochnogo i gumoral'nogo immuniteta i morfologiiu éritrotsitov u bol'nykh ishemicheskoi bolezn'iu serdtsa.

A study is presented of the effect of reinfusions of ultraviolet-irradiated autologous blood on the number of lymphocytes, titer of circulating immune complexes and theophylline-sensitive cells, number of immunoglobulins A, G and M, number and form of erythrocytes in 30 patients with stable and progressive exertion stenocardia. Thirty control patients were treated with nitrates, calcium antagonists and acetylsalicylic acid. It was established that in patients with stenocardia quantum hemotherapy produces an immunocorrective action reduces the titer of circulating immune complexes, the content of T-lymphocytes, the number of immunoglobulins A and G, normalizes the morphological and functional activity of erythrocytes. Use of this method in the treatment of ischemic heart disease is pathogenetically justified and prevents development of myocardial infarction.

[The mechanism of the action of UV-irradiated autotransfusions in treating patients with suppurative-inflammatory diseases of the face and neck]. / O mekhanizme deistviia transfuzii UFO-autokrovi pri lechenii bol'nykh s gnoino-vospalitel'nymi zabolevaniiami litsa i shei.

An analysis of examination and treatment of 36 patients with acute pyo-inflammatory diseases of face and neck has shown the inclusion of UV-irradiated autotransfusions into the complex of therapeutic measures to decrease blood viscosity and to increase deformability of erythrocytes. It elevates the intensity of blood circulation in the system of macro- and microcirculation. The increased arteriovenous oxygen difference after UV-irradiated autotransfusions points to improved utilization of tissue blood and enhanced bioenergetic processes.

[Systemic effects of ultraviolet, visible and infrared radiation in serial whole body irradiation. I. Oxygen utilization, flow properties of blood, hemodynamics, blood components and phagocytosis]. / Systemische Effekte der Ultraviolett-, sichtbaren bzw. Infrarotstrahlung bei seriellen Ganzkörperbestrahlungen. I. Sauerstoffutilisation, Fliesseigenschaften des Blutes, Hämodynamik, Blutbestandteile und Phagozytose.

31 healthy volunteers at the age of 19 to 29 years underwent whole-body irradiations by either ultraviolet radiation (NARVA UVS 65-2; continuous sunshine-like emission, predominantly UVA plus 8% UVB; cumulative doses after 4 and 20 irradiations 8.8 J/cm2 and 51.0 J/cm2, respectively), or visible light (emission of a 3,500 Watt lamp HGMI 3500 DL, Tungsram CSSR, filtered through 6 mm window glass; cumulative dose 267.0 J/cm2 after 4 irradiations) or infrared radiation (250 Watt infrared lamps NARVA "Biotherm", emitting more than 70% infrared radiation; cumulative dose 159.0 J/cm2 after 4 irradiations). Before, during and after the irradiation series the following investigations were made: Arterial and venous oxygen pressure, oxygen utilization index; flow properties of peripheral blood (plasma viscosity, erythrocyte aggregation kinetics, apparent blood viscosity); hemodynamics (veineal plethysmography, 133Xenon clearance, functional diameter of small blood vessels, peripheral blood pressure), phagocytotic capacity of polymorphonuclear white blood cells; hematological parameters (blood sedimentation rate, polymorphonuclear and eosinophilic blood cells, red blood cells, hemoglobin, hematocrit), serum proteins (IgG, IgA, IgM, complement C3, alpha-1-glycoprotein, alpha-1-antitrypsin, haptoglobin, transferrin); calcium and phosphate in serum. As far as irradiance, dose and treatment frequencies are concerned, the experimental conditions were very similar to those in phototherapeutical practice. Under these circumstances there were no hints for unwanted early effects after application of all the three kinds of optical radiation used in that study. Considerable systemic responses exclusively were found by use of ultraviolet radiation. Under these experimental conditions according to dermatological phototherapy it has been proven the following biopositive systemic responses are due to happen: increase of serum calcium and phosphate, improvement of blood oxygen utilization, improvement of blood flow properties, and enhanced phagocytic capacity of polymorphonuclear white blood cells. Regarding the parameters taken in that study, no early unwanted side effects are to expect after therapeutical application of rather large doses of visible light or infrared radiation.

[Functional and structural changes in the surface of human erythrocytes after UV irradiation with rays of various wavelengths. IV. Changes in the physicochemical properties of autotransfused UV-irradiated blood]. / Funktsional'nye i strukturnye izmeneniia poverkhnosti éritrotsitov cheloveka posle oblucheniia UF luchami raznoi dliny volny. IV. Izmeneniia fiziko-khimicheskikh svoistv pri autotransfuziiakh UF-obluchennoi krovi.

Properties of erythrocyte surface were investigated for patients with ischemic heart disease in the course of treatment with the UV-irradiated blood autotransfusion (UVIBA). Application of methods of light-scattering, photometry and cytochemistry revealed rapid and significant changes in deformability and aggregation properties of the erythrocytes immediately following each UVIBA procedure, which was accompanied by considerable blood viscosity decrease.