ABSTRACT
Abstract In this research, aqueous and ethanolic extracts from Justicia pectoralis Jacq and Croton Jacobinensis Baill were characterized. The UPLC-QTOF-MSE analysis was performed on the extracts identified, predominantly, flavonoids, tannins and acids. The extracts did not indicate toxicity in human epithelial cells. C. jacobinensis presented a concentration of phenolics 60.5% higher than J. pectoralis in all scenarios evaluated and, for both samples, the hydroalcoholic extract at 70% exhibited the best efficiency in the extraction (14501.3 and 32521.5 mg GAE 100 g-1 for J. pectoralis and C. jacobinensis, respectively). The antioxidant activity presented a positive correlation with the concentration of phenolics, being 1.186,1 and 1.507,9 µM of Trolox for J. pectoralis and C. jacobinensis at 70% of ethanol; however, it was not verified statistical difference between the ethanolic solutions (p < 0.05). The antimicrobial activity of J. pectoralis extracts was highlighted once was the most effective against gram-positive bacteria. The results suggest that both J. pectoralis and C. jacobinensis extracts present the potential to be applied as natural additives due to their antioxidant and antimicrobial activity and safety. Thus, it is suggesting the development of studies that could investigate the interaction of these plant extracts with food matrices is required
Subject(s)
Plant Extracts/analysis , Euphorbiaceae/classification , Social Justice/classification , Croton/classification , Toxicity , Antioxidants/analysis , Flavonoids/analysis , Phytochemicals/adverse effects , Gram-Positive Bacteria/metabolismABSTRACT
A unique collection of 292 extracts from 107 New Caledonian Euphorbiaceae species sensu lato was profiled by LC-MS2 and the metabolite content organized by molecular networking. Based on the assumption that taxon-specific molecules are more likely to be structurally novel, taxonomic data were mapped on spectral networks to detect genus-specific clusters. Using this approach, a group of compounds unique to the genus Austrobuxus was highlighted. The subsequent MS-guided purification of the fruit EtOAc extract of Austrobuxus carunculatus led to the isolation of 13 new monolactone and "norditerpene" picrotoxanes (2-14), along with the known tutin (1). The structures of the new compounds were elucidated by HRESIMS and NMR spectroscopic data analysis, and the absolute configurations of compounds 1, 3, 7, 11, 12, and 14 were determined by single-crystal X-ray diffraction analysis. The relative and absolute configurations of compounds 4 and 5 were ascertained by chemical transformation of compound 3. The absolute configurations of other members of the series have been proposed on the basis of biogenetic considerations and specific rotation values of similar sign and magnitude. Compounds 1-14 were evaluated for their antiproliferative activities against HCT116 colon, U87-MG glioblastoma, and A549 lung human cancer cell lines. Compounds bearing an acyl chain at C-2 (i.e., 2, 4, and 13) showed IC50 values in the micromolar range for the three cell lines used.
Subject(s)
Antineoplastic Agents, Phytogenic/isolation & purification , Euphorbiaceae/chemistry , Euphorbiaceae/classification , Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/pharmacology , Cell Line, Tumor , Cell Proliferation/drug effects , Classification , Diterpenes , Humans , Magnetic Resonance Spectroscopy , Mass Spectrometry , Metabolic Networks and Pathways , Molecular Structure , Plant Extracts/chemistry , Plant Extracts/pharmacology , Spectrometry, Mass, Electrospray Ionization , Structure-Activity Relationship , X-Ray DiffractionABSTRACT
Cnidoscolus chayamansa is a native plant of the Mayan region, which is also cultivated in other places like northern Mexico, Tunisia and India. Many properties are attributed to Mayan Chaya, such as aid in the control of glycemia in diabetics. Thus this study aimed to evaluate the hypoglycemic effects of chaya aqueous extracts in a model of streptozotisin-induced diabetic Wistar rats. Chaya aqueous extracts were collected from plants cultivated in Quinta Roo (Mayan region) and Durango (northern Mexico), and in this study we compare their effect with metformin (as a control). Additionally, we compared the extracts mass profiles from both regions by high-resolution liquid chromatography coupled to a triple quadrupole tandem mass detector (HPLC-MS/MS QQQ). Finally, a study of the pancreatic tissue was carried out to evaluate the effects of the extracts on the Langerhans islets. Both extracts showed a good hypoglycemic effect after two weeks of treatment, and the Langerhans islets showed a partial recovery due to the effect of the treatment. Although the plants were cultivated at a distance of 2,350 km and under different weather, the compounds found in both did not show significant differences.
Subject(s)
Animals , Female , Rats , Plant Extracts/adverse effects , Streptozocin/administration & dosage , Euphorbiaceae/classification , Diabetes Mellitus/chemically induced , Hyperglycemia , Hypoglycemic Agents/adverse effects , Plants , Chromatography, High Pressure Liquid/methods , Islets of LangerhansABSTRACT
BACKGROUND: Cnidoscolus is a genus belonging to the Euphorbiaceae family, distributed in South American countries, such as Mexico and Brazil, which includes several species widely used in folk medicine. However, the genus is not sufficiently exploited from a chemical and pharmacological point of view. HYPOTHESIS/PURPOSE: This paper aims to present a systematic review of known pharmacological and chemical aspects from Cnidoscolus, an important genus for South America research groups on medicinal plants. In this article, we highlight the importance of Cnidoscolus species in the search for new bioactive molecules. STUDY DESIGN: A systematic review was conducted in order to collect chemical and pharmacological information on species of this genus in the last 25 years. METHODS: Literature search was performed through specialized databases (PUBMED, LILACS, SCIELO, Science Direct and Web of Science) using different combinations of the following keywords: Cnidoscolus, phytochemistry, pharmacological activity. For the selection of the manuscripts, two independent investigators (RGOJ and CAAF) first selected the articles according to the title, then to the abstract and finally through an analysis of the full-text publication. All selected manuscripts were analyzed for year of publication, country where the research was performed, reported plant species, isolated chemical compounds and evaluated biological activities. RESULTS: Most of the studies involving Cnidoscolus were conducted by research groups located in Brazil, Nigeria and Mexico. Regarding the annual evolution of the publications, a larger number of articles published in 2014 were observed. Flavonoids, triterpenes and diterpenes represent the main classes of secondary metabolites that have been isolated from Cnidoscolus. In terms of the pharmacological investigations, C. aconitifolius, C. chayamansa and C. quercifolius are considered the most studied species, with different pharmacological activities. CONCLUSION: All findings indicate that Cnidoscolus is an important genus of the Euphorbiaceae family. However, considering its chemical and pharmacological importance, the studies developed with Cnidoscolus species are still limited, representing an opportunity to investigate new bioactive molecules.
Subject(s)
Euphorbiaceae/classification , Phytochemicals/pharmacology , Animals , Brazil , Ethnopharmacology , Humans , Medicine, Traditional , Mexico , Phytotherapy , Plant Extracts/pharmacology , Plants, Medicinal/chemistryABSTRACT
A comprehensive overview of natural orbitides isolated from Euphorbiaceae species and their most relevant biological activities are presented. Euphorbiaceae is a large and diverse family, which comprises about 300 genera, and is known as an important source of medicines and toxins. Several classes of secondary metabolites have been described for this taxon, however, orbitides have been broadly reported in Jatropha and Croton genera. Additionally, the latex is documented as the main source of orbitides in this family. Based on their structural and functional diversity, orbitides present a large variety of biological activities described as cytotoxicity, antimalarial, antibacterial, antifungal, enzymatic inhibition, and immunosuppressive, although the mechanism of action still needs to be further investigated. In recent years, the discovery of bioactive cyclic peptides from different sources has grown exponentially, making them promising molecules in the search for new drug leads. This review also highlights the attempts made by many researchers to organize the orbitides nomenclature and amino acid numbering, as well the important progress recently achieved in the biosynthetic study area.
Subject(s)
Anti-Infective Agents/pharmacology , Biological Products/pharmacology , Euphorbiaceae/chemistry , Immunosuppressive Agents/pharmacology , Peptides, Cyclic/pharmacology , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/isolation & purification , Anti-Bacterial Agents/pharmacology , Anti-Infective Agents/chemistry , Anti-Infective Agents/isolation & purification , Antifungal Agents/chemistry , Antifungal Agents/isolation & purification , Antifungal Agents/pharmacology , Antimalarials/chemistry , Antimalarials/isolation & purification , Antimalarials/pharmacology , Biological Products/chemistry , Biological Products/isolation & purification , Croton/chemistry , Croton/classification , Croton/metabolism , Euphorbiaceae/classification , Euphorbiaceae/metabolism , Immunosuppressive Agents/chemistry , Immunosuppressive Agents/isolation & purification , Jatropha/chemistry , Jatropha/classification , Jatropha/metabolism , Peptides, Cyclic/chemistry , Peptides, Cyclic/isolation & purificationABSTRACT
Croton scarciesii (Euphorbiaceae-Crotonoideae), a rheophytic shrub from West Africa, is shown to have been misplaced in Croton for 120 years, having none of the diagnostic characters of that genus, but rather a set of characters present in no known genus of the family. Pollen analysis shows that the new genus Karima belongs to the inaperturate crotonoid group. Analysis of a concatenated molecular dataset combining trnL-F and rbcL sequences positioned Karima as sister to Neoholstia from south eastern tropical Africa in a well-supported clade comprised of genera of subtribes Grosserineae and Neoboutonieae of the inaperturate crotonoid genera. Several morphological characters support the relationship of Karima with Neoholstia, yet separation is merited by numerous characters usually associated with generic rank in Euphorbiaceae. Quantitative ecological data and a conservation assessment supplement illustrations and descriptions of the taxon.
Subject(s)
Euphorbiaceae/genetics , Genes, Plant , Africa, Western , Euphorbiaceae/classification , Microscopy, Electron, Scanning , Phylogeny , Pollen/ultrastructureABSTRACT
RESUMO A cada dia, cepas bacterianas estão tornando-se resistentes a diversos antibióticos, o que faz necessária a busca de novas substâncias eficazes para o tratamento de doenças. Desta forma, este trabalho reporta o estudo preliminar toxicológico, antibacteriano e fitoquímico do extrato etanólico das folhas de Jatropha mollissima (pinhão-bravo, Euphorbiaceae), coletada no Município de Tauá, Ceará, Nordeste Brasileiro. Inicialmente, realizou-se o teste de toxicidade do extrato contra Artemia salina. Na sequencia, foi realizado o ensaio antibacteriano contra quatro cepas bacterianas Gram-negativas (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Hafnia alvei ATCC 51873, Klebsiella pneumoniae ATCC 13883) e uma cepa Gram-positiva (Enterococcus faecalis ATCC 29212). Finalmente, fez-se a análise fitoquímica preliminar do extrato ativo para detecção das principais classes de metabólitos especiais. Como resultado, o extrato etanólico das folhas de J. mollissima se mostrou tóxico para Artemia salina, pois apresentou CL50 igual a 406,02 μg/mL. Quanto à ação antibacteriana, o extrato se mostrou ativo contra a bactéria Gram-positiva Enterococcus faecalis ATCC 29212, apresentando moderada atividade antibacteriana (halo de inibição igual a 7,03 mm). Evidenciou-se no extrato bioativo a presença de cumarinas, fenóis, taninos, flavonoides (flavonóis e flavanonas), alcaloides e esteroides, ambas as classes reportadas como antimicrobianos. Portanto, esse extrato tem potencial para ser usado na produção de fármacos contra infecções causadas por bactérias Gram-positivas. No entanto, as informações direcionam estudos futuros para o isolamento e identificação dos compostos bioativos, monitorados sob a ação antibacteriana mais expressiva.
ABSTRACT Each day, bacterial strains are becoming more resistant to various antibiotics, which requires the search for new effective substances for the treatment of diseases. Thus, this study reports the toxicological, antibacterial, and phytochemical preliminary study of the ethanolic extracts of Jatropha mollissima (pinhão-bravo, Euphorbiaceae) leaves, collected in Tauá, Ceará, Northeast of Brazil. Initially, we performed the toxicity testing of the extract against Artemia salina. Then, we conducted the antibacterial assay against four Gram-negative bacterial strains (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Hafnia alvei ATCC 51873, Klebsiella pneumoniae ATCC 13883), and one Gram-positive strain (Enterococcus faecalis ATCC 29212). Finally, we carried out the preliminary phytochemical analysis of the active extract to detect the main classes of special metabolites. As a result, the ethanolic extract of J. mollissima leaves was toxic to Artemia salina, because it presented LC50 equal to 406.02 µg/mL. Regarding antibacterial action, the extract was active against the Gram-positive bacteria Enterococcus faecalis ATCC 29212, with moderate antibacterial activity (inhibition zone equal to 7.03 mm). The bioactive extract had the presence of coumarins, phenols, tannins, flavonoids (flavanols and flavonones), alkaloids and steroids, both classes reported as antimicrobials. Therefore, this extract has the potential to be used in the production of drugs against infections caused by Gram-positive bacteria. However, these information require further studies for the isolation and identification of bioactive compounds, monitored under the more expressive antibacterial action.
Subject(s)
Toxicity Tests/methods , Euphorbiaceae/classification , Anti-Bacterial Agents , Artemia/classification , Enterococcus faecalis/classificationABSTRACT
Croton zehntneri (Euphorbiaceae) is a native aromatic plant from Northeast region of Brazil. The monoterpenoid estragole (ESL) has been isolated by classical chromatographic methods from the essential oil (EO) of C. zehnteneri leaves and characterized by GC-FID and GC-MS, its antimicrobial and cytotoxic potentials being assessed. The analysis of the EO enabled the identification of 100% of the integrated constituents, of which yield was about 1.8%. The main components identified were: eucalyptol, estragole (84.7%) and spathulenol. The dosage of 50 µg/disk of ESL presented fairly significant zones of inhibition against Gram-positive bacteria and fungi. The ESL presented toxicity against Artemia salina with LC50 and LC90 of 4,54 and 8,47 µg mL-1. However, in tumor inhibition assays (human cells), there were no rewarding inhibition in any of the human cancer cell lines (MCF-7, HEP-2 and NCI-H292).
Subject(s)
Anisoles/pharmacology , Anti-Infective Agents/pharmacology , Antineoplastic Agents, Phytogenic/pharmacology , Croton Oil/chemistry , Cyclohexanols/pharmacology , Euphorbiaceae/chemistry , Monoterpenes/pharmacology , Oils, Volatile/chemistry , Allylbenzene Derivatives , Anisoles/isolation & purification , Anti-Infective Agents/isolation & purification , Cell Line, Tumor/drug effects , Cyclohexanols/isolation & purification , Disk Diffusion Antimicrobial Tests , Drug Screening Assays, Antitumor , Eucalyptol , Euphorbiaceae/classification , Fungi/drug effects , Gas Chromatography-Mass Spectrometry , Gram-Negative Bacteria/drug effects , Gram-Positive Bacteria/drug effects , Humans , Monoterpenes/isolation & purificationABSTRACT
A physico-chemical and phytochemical characterisation of the oil of two rich sources of polyunsaturated fatty acids, tocopherols and phytosterols is presented for two close species of Plukenetia, endemic to the Amazon Region of Peru. Plukenetia huayllabambana presented approximately 9% more oil yield than Plukenetia volubilis. Fatty acid profiles were pretty similar for both species but P. huayllabambana presented a significantly higher content of α-linolenic acid than P. volubilis (51.3 and 45.6 g/100 g oil, respectively). Important contents of γ- and δ-tocopherol were evidenced in both oils (127.6 and 84.0 and, 93.3 and 47.5 mg/100 g oil, for P. volubilis and P. huayllabambana, respectively). ß-Sitosterol was the most important and representative phytosterol in both oils (â¼127 mg/100 g oil). The results of this study indicate P. huayllabambana as an important dietary source of health promoting phytochemicals.
Subject(s)
Euphorbiaceae/chemistry , Euphorbiaceae/classification , Phytochemicals/analysis , Plant Oils/chemistry , Fatty Acids, Omega-3/analysis , Fatty Acids, Omega-6/analysis , Peru , Sitosterols/analysis , Tocopherols/analysis , alpha-Linolenic Acid/analysisABSTRACT
ABSTRACT The antibacterial activity of the alkaloid extract from the leaves of Croton bonplandianum Baill. and its main compounds, sparsiflorine and crotsparine, was tested against Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa by the resazurin microtitre-plate method. Pure compounds were identified by spectroscopic techniques, mainly 1D and 2D NMR. The alkaloid extract showed activity particularly against the S. aureus and P. aeruginosa. Regarding the pure compounds, the crotsparine was inactive against the microorganisms assayed, whereas the sparsiflorine indicated a moderate activity similar to the alkaloid extract. The Pseudomonas aeruginosa was the most sensitive of the tested microorganisms with MIC of 0.141 mg/mL. The results suggest that the activity of the extract may be credited mainly to the presence of the sparsiflorine. Although the activity of the sparsiflorine does not get close to the antimicrobial drugs in clinical use, it still could be a lead compound for the development of new antibacterial substances.
RESUMO A atividade antibacteriana do extrato alcaloidal das folhas de Croton bonplandianum Baill., além dos principais compostos isolados, esparsiflorina e crotsparina, foi testada contra Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae e Pseudomonas aeruginosa. Foi utilizado o método de microdiluição em placa empregando resazurina como indicador da viabilidade celular. Os compostos isolados foram identificados por técnicas espectroscópicas, principalmente RMN 1D e 2D. O extrato alcaloidal foi ativo principalmente contra S. aureus e P. aeruginosa. Crotsparina mostrou-se inativa contra todos os micro-organismos testados, enquanto esparsiflorina apresentou atividade moderada, a qual foi similar à do extrato bruto. Pseudomonas aeruginosa foi a mais sensível das bactérias testadas, com CIM de 0,141 mg/mL. Os resultados sugerem que a atividade do extrato pode ser devida em grande medida pela presença de esparsiflorina. Apesar de a CIM da esparsiflorina não ter se aproximado daquela apresentada pelos agentes antimicrobianos em uso clínico, tal composto ainda pode compor um protótipo interessante para o desenvolvimento de novas substâncias antibacterianas.
Subject(s)
Euphorbiaceae/classification , Alkaloids/analysis , Anti-Bacterial Agents/analysis , Plant Extracts , Staphylococcus Phages , Croton/classificationABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE: Latices from several plant species of Euphorbiaceae family have been traditionally applied over fresh cuts to stop bleeding and subsequently applied over wounds to enhance healing process. The latex arrested bleeding from fresh wounds by reducing bleeding and whole blood coagulation time which are important indices of hemostatic activity. It has been accepted that hemostatic activity is due to the proteolytic fraction of plant latices. Thus, the present study aimed to assess the clot inducing properties of three Euphorbiaceae plants viz., Euphorbia nivulia Buch.-Ham., Pedilanthus tithymaloides (L.) Poit and Synadenium grantii Hook F. MATERIALS AND METHODS: In the present study, various proteolytic activities namely protease, gelatinase, milk clotting and whole blood clotting assay of the enzyme fraction of latices of Euphorbia nivulia, Pedilanthus tithymaloides and Synadenium grantii have been investigated. The inhibition profile of protease specific inhibitors was assessed. Also, the effects of protein fractions were studied using bleeding/clotting time test of fresh experimentally-induced wounds in mice. RESULTS: Euphorbia nivulia latex protease has noticeable blood clotting activity followed by Pedilanthus tithymaloides and Synadenium grantii. Stem latex protease of Pedilanthus tithymaloides exhibits superior procoagulant activity in different mammal's blood samples viz., Capra hircus, Bubalus bubalis, Ovibos moschatus and Bos indicus. Blood sample of ox was the most sensitive to latex protease than other mammal's blood. Concomitantly, the plant latex protease could significantly reduce whole blood clotting time of human and mice blood samples. CONCLUSION: The protease fraction of latices of Euphorbia nivulia, Pedilanthus tithymaloides and Synadenium grantii possesses phytoconstituents capable of arresting wound bleeding, and accelerating whole blood coagulation process. It suggests good potentiality for use of latex proteases in wound management. Also, the finding of this study showed that the protease enzyme of Pedilanthus tithymaloides has the most potent hemostatic agent.
Subject(s)
Blood Coagulation/drug effects , Euphorbiaceae/chemistry , Euphorbiaceae/classification , Latex/pharmacology , Latex/toxicity , Animals , Dermatitis, Allergic Contact , Hemostatics/chemistry , Hemostatics/pharmacology , MiceABSTRACT
The methanol extracts of three Macaranga species (M. denticulata, M. pruinosa, and M. gigantea) were screened to evaluate their total phenolic contents and activities as cholinesterase inhibitors, nitric oxide (NO) production inhibitors, tyrosinase inhibitors, and antioxidants. The bark of M. denticulata showed the highest total phenolic content (2682 mg gallic acid equivalent (GAE)/100 g) and free radical scavenging activity (IC50 = 0.063 mg/mL). All of the samples inhibited linoleic acid peroxidation by greater than 80%, with the leaves of M. gigantea exhibiting the highest inhibition of 92.21%. Most of the samples exhibited significant antioxidant potential. The bark of M. denticulata and the leaves of both M. pruinosa and M. gigantea exhibited greater than 50% tyrosinase inhibition, with the bark of M. denticulata having the highest percentage of inhibition (68.7%). The bark and leaves of M. denticulata exhibited greater than 50% inhibition (73.82% and 54.50%, resp.) of the acetylcholinesterase enzyme (AChE), while none of the samples showed any significant inhibition of butyrylcholinesterase (BChE). Only the bark of M. denticulata and M. gigantea displayed greater than 50% inhibition of nitric oxide production in cells (81.79% and 56.51%, resp.). These bioactivities indicate that some Macaranga spp. have therapeutic potential in medicinal research.
Subject(s)
Antioxidants/chemistry , Cholinesterase Inhibitors/chemistry , Euphorbiaceae/chemistry , Euphorbiaceae/metabolism , Monophenol Monooxygenase/antagonists & inhibitors , Nitric Oxide/antagonists & inhibitors , Plant Extracts/chemistry , Cholinesterase Inhibitors/pharmacology , Enzyme Activation , Euphorbiaceae/classification , Phenols/chemistry , Plant Bark/chemistry , Plant Extracts/pharmacology , Plant Leaves/chemistry , Species SpecificityABSTRACT
Fatty acids (FA), phytosterols, tocopherols, phenolic compounds, total carotenoids and hydrophilic and lipophilic ORAC antioxidant capacities were evaluated in 16 cultivars of Sacha inchi (SI) seeds with the aim to valorise them and offer more information on the functional properties of SI seeds. A high α linolenic (α-Ln) fatty acid content was found in all cultivars (ω3, 12.8-16.0 g/100 g seed), followed by linoleic (L) fatty acid (ω6, 12.4-14.1g/100g seed). The ratio ω6/ω3 was within the 0.83-1.09 range. γ- and δ-tocopherols were the most important tocopherols, whereas the most representative phytosterols were ß-sitosterol and stigmasterol. Contents of total phenolics, total carotenoids and hydrophilic and lipophilic antioxidant capacities ranged from 64.6 to 80 mg of gallic acid equivalent/100g seed; from 0.07 to 0.09 mg of ß-carotene equivalent/100g of seed; from 4.3 to 7.3 and, from 1.0 to 2.8 µmol of Trolox equivalent/g of seed, respectively, among the evaluated SI cultivars. Results showed significant differences (p<0.05) among the evaluated SI cultivars in the contents of ω3, ω6, antioxidant capacities and other evaluated phytochemicals. SI seeds should be considered as an important dietary source of health promoting phytochemicals.
Subject(s)
Antioxidants/analysis , Euphorbiaceae/chemistry , Fatty Acids, Unsaturated/analysis , Phenols/analysis , Phytosterols/analysis , Tocopherols/analysis , Euphorbiaceae/classification , Plant Oils/chemistry , Seeds/chemistryABSTRACT
Phyllanthus niruri L. (Euphorbiaceae), known as "quebra-pedra" (Portuguese for "stonebreaker"), is an herb used for kidney disorders. In light of its frequent use by the population, the present study aimed to investigate the genotoxic, antigenotoxic and cytotoxic activities of a standardized P. niruri extract in bone marrow rats. Three groups of 12 animals were treated daily by gavage over a period of 30 days, with 50, 150 or 250 mg/kg of P. niruri extract aqueous solution. The control group (n = 12) received tap water. At the end of treatment (day 31), groups were divided into two minor subgroups (n=6/group) and received cyclophosphamide (50 mg/kg, i.p.) or saline 0.9% (i.p.). After 24 hours, we evaluated the frequency of micronucleated polychromatic erythrocytes for each animal (MNPCE) at 1000 PCE. Cytotoxicity was evaluated with the PCE/NCE ratio (NEC = normochromatic erythrocytes). General toxicity was assessed during treatment using the parameters of body weight gain, ration and water consumption. The dry extract did not provoke changes in body weight, weight gain, ration and water intake or changes in the frequency of MNPCE or cytotoxicity in bone marrow. We propose that the P. niruri extract used here showed no genotoxic, antigenotoxic and cytotoxic activities under the experimental conditions.
Phyllanthus niruri L. (Euphorbiaceae), conhecida como "quebra-pedra", é uma planta medicinal utilizada frequentemente pela população no tratamento de problemas renais. Foram avaliadas as atividades genotóxicas, antigenotóxicas e citotóxicas de um extrato padronizado dessa espécie em ratos. Três grupos de doze animais foram tratados durante trinta dias, por gavagem, com 50, 150 ou 250 mg/kg/dia de solução aquosa do extrato de P. niruri e um grupo controle (n=12) recebeu água destilada pela mesma via. No final do tratamento os grupos foram divididos em dois subgrupos (6 animais/grupo) e receberam uma dose única de ciclofosfamida (50 mg/kg, i.p.) ou de solução salina 0,9% (i.p.). Após 24 horas, a frequência de eritrócitos policromáticos micronucleados (EPCMN) foi avaliada em 1000 EPC. A citotoxicidade foi avaliada pela relação entre eritrócitos policromáticos e normocromáticos (EPC/ENC) e a toxicidade geral foi avaliada através dos parâmetros de ganho de peso corporal, consumo de ração e ingestão hídrica. O extrato seco não provocou alterações significativas no peso corporal, ganho de peso e consumo de ração em relação ao grupo controle, nem alterações na frequência de EPCMN ou citotoxicidade em medula óssea. Dessa maneira, pode-se concluir que P. niruri não apresentou atividades genotóxica, antigenotóxica e/ou citotóxica nas condições experimentais executadas.
Subject(s)
Rats , Bone Marrow/physiopathology , Micronucleus Tests/classification , Euphorbiaceae/classification , Genotoxicity/analysis , Plants, Medicinal/anatomy & histology , Plants, Medicinal/metabolismABSTRACT
Although much attention has been paid to the role of stabilizing selection, empirical analyses testing the role of developmental constraints in evolutionary stasis remain rare, particularly for plants. This topic is studied here with a focus on the evolution of a pollen ontogenetic feature, the last points of callose deposition (LPCD) pattern, involved in the determination of an adaptive morphological pollen character (aperture pattern). The LPCD pattern exhibits a low level of evolution in eudicots, as compared to the evolution observed in monocots. Stasis in this pattern might be explained by developmental constraints expressed during male meiosis (microsporogenesis) or by selective pressures expressed through the adaptive role of the aperture pattern. Here, we demonstrate that the LPCD pattern is conserved in Euphorbiaceae s.s. and that this conservatism is primarily due to selective pressures. A phylogenetic association was found between the putative removal of selective pressures on pollen morphology after the origin of inaperturate pollen, and the appearance of variation in microsporogenesis and in the resulting LPCD pattern, suggesting that stasis was due to these selective pressures. However, even in a neutral context, variation in microsporogenesis was biased. This should therefore favour the appearance of some developmental and morphological phenotypes rather than others.
Subject(s)
Euphorbiaceae/physiology , Evolution, Molecular , Glucans/metabolism , Pollen/physiology , Selection, Genetic , Adaptation, Physiological , Biological Evolution , Cell Wall/physiology , Cytokinesis , Euphorbiaceae/classification , Euphorbiaceae/cytology , Gametogenesis, Plant , Phenotype , Phylogeny , Pollen/classification , Pollen/cytology , Species SpecificityABSTRACT
Mercurialis annua and M. perennis are medicinal plants used in complementary medicine. In the present work, analytical methods to allow a chemotaxonomic differentiation of M. annua and M. perennis by means of chemical marker compounds were established. In addition to previously published compounds, the exclusive presence of pyridine-3-carbonitrile and nicotinamide in CH(2) Cl(2) extracts obtained from the herbal parts of M. annua was demonstrated by GC/MS. Notably, pyridine-3-carbonitrile was identified for the first time as a natural product. Further chromatographic separation of the CH(2) Cl(2) extracts via polyamide yielded a MeOH fraction exhibiting a broad spectrum of side-chain saturated n-alkylresorcinols. While the n-alkylresorcinol pattern was similar for both plant species, some specific differences were observed for particular n-alkylresorcinol homologs. Finally, the investigation of H(2) O extracts by LC/MS/MS revealed the presence of depside constituents. Whereas, in M. perennis, a mixture of mercurialis acid (=(2R)-[(E)-caffeoyl]-2-oxoglutarate) and phaselic acid (=(E)-caffeoyl-2-malate) could be detected, in M. annua solely phaselic acid was found. By comparison with synthesized enantiomerically pure (2R)- and (2S)-phaselic acids, the configuration of the depside could be determined as (2S) in M. annua and as (2R) in M. perennis.
Subject(s)
Euphorbiaceae/chemistry , Euphorbiaceae/classification , Ketoglutaric Acids/isolation & purification , Medicine, Traditional , Niacinamide/isolation & purification , Nitriles/isolation & purification , Plant Extracts/isolation & purification , Pyridines/isolation & purification , Chromatography, High Pressure Liquid , Gas Chromatography-Mass Spectrometry , MetabolomeABSTRACT
In this study, we tested the applicability of four DNA regions (rbcL, matK, ITS, and ITS2) as barcodes for identifying species within Euphorbiaceae. Based on assessments of the specific genetic divergence, the DNA barcoding gap, and the ability for species discrimination, the present results affirmed that ITS/ITS2 is a potential barcode for the Euphorbiaceae species. This study also provided a large-scale test to evaluate the effectiveness of ITS/ITS2 for differentiating species within Euphorbiaceae. Of the 1183 plant samples collected from 871 species in 66 diverse genera, ITS/ITS2 successfully identified > 90% and 100% of them at the species and genus levels, respectively. Therefore, our research indicates that use of the ITS/ITS2 region is a powerful technique for Euphorbiaceae identification.
Subject(s)
DNA Barcoding, Taxonomic , Euphorbiaceae/classification , Classification/methods , DNA, Intergenic/chemistry , Euphorbiaceae/genetics , Genes, Plant , Species SpecificityABSTRACT
A study of microsporogenesis (the earliest stage of pollen ontogeny) was undertaken in seven cultivars of Codiaeum variegatum var. pictum, a eudicot species that produces inaperturate pollen grains. Microsporogenesis appears highly variable for the developmental events suspected to be implicated in the determination of aperture pattern. Most eudicots have tri-aperturate pollen grains and microsporogenesis is described as highly conserved in this clade. The observed burst of variation in C. variegatum therefore appears especially remarkable. A plausible hypothesis to explain the variation is that the pollen being inaperturate, the selective forces applying on the ontogeny of the aperture pattern are relaxed.
Subject(s)
Euphorbiaceae/anatomy & histology , Euphorbiaceae/genetics , Euphorbiaceae/classification , Meiosis , Pollen/anatomy & histologyABSTRACT
A espécie Sapium glandulatum, conhecida vulgarmente como leiteiro, é uma das espécies nativas potencialmente recomendada para a recuperação de áreas degradadas, devido principalmente ao seu caráter pioneiro, além de possivelmente possuir características medicinais, uma vez que a família Euphorbiaceae, a qual pertence, é rica em compostos fenólicos. Em abril/2000 e dezembro/2000 foram conduzidos experimentos em casa-de-vegetação para verificar o efeito da aplicação de diferentes concentrações de ácido indolilbutírico (AIB), em solução concentrada (10 segundos de imersão), solução diluída (16 horas de imersão) e em talco, associadas ou não ao ácido bórico, no enraizamento de estacas semilenhosas de Sapium glandulatum. As estacas foram confeccionadas a partir de brotações do ano, coletadas de plantas matrizes localizadas no município de Bocaiúva do Sul -PR, mantendo- as com comprimento de cerca de 10 cm e 2 folhas apicais reduzidas à metade. Os tratamentos utilizados foram: 0, 4000, 6000 e 8000 mg L-1 de AIB, sozinhos e em associação com 150 mg L-1 de ácido bórico (solução concentrada), 0, 200 e 400 mg L-1 de AIB, sozinhos e em associação com 150 mg L-1 ácido bórico (solução diluída) e 0, 4000, 6000 e 8000 mg L-1 de AIB, na forma de talco. Pelos resultados obtidos foi possível concluir que, após 70 dias em casa-de-vegetação, a melhor época para coleta das estacas correspondeu à instalação realizada em dezembro/2000 (verão), onde o tratamento com 8000 mg L-1 de AIB, em solução concentrada, proporcionou o maior percentual de enraizamento (14 por cento), porém não diferindo significativamente dos demais tratamentos. A utilização da solução diluída não se mostrou promissora para a indução do sistema radicial devido à alta mortalidade das estacas. Os tratamentos com AIB veiculados em talco não superaram os resultados obtidos pelos melhores tratamentos para estacas enraizadas em solução concentrada, não sendo um método recomendável para o aumento da indução radicial de Sapium glandulatum. Pelo exposto, pode-se considerar que estacas de brotações do ano desta espécie não são indicadas para sua propagação vegetativa, de acordo com os tratamentos realizados.
The species Sapium glandulatum, commonly known as "leiteiro", is one of the Brazilian native species potentially recommended for the recovery of degraded areas, mainly due to its pioneering character and probable medicinal properties since its family, Euphorbiaceae, is rich in phenolic compounds. In April/2000 and December/2001, experiments were carried out in a greenhouse to investigate the effects of different indolebutyric acid (IBA) levels in concentrated solution (10 seconds immersion), diluted solution (16 hours immersion) and as talc, associated or not with boric acid, on the rooting of Sapium glandulatum semi-hardwood cuttings. The latter were produced from sproutings of the year collected from stock plants located in Bocaiúva do Sul, Paraná State, Brazil. The length of each cutting was about 10 cm, with 2 half apical leaves. The following treatments were evaluated: 0, 4000, 6000 and 8000 mg L-1 IBA, alone and with 150 mg L-1 boric acid (concentrated solution); 0, 200 and 400 mg L-1 IBA, alone and with 150 mg L-1 boric acid (diluted solution); and 0, 4000, 6000 and 8000 mg L-1 IBA as talc. After 70 days in greenhouse, the best period for cutting collection was December/2000 (summer), and 8000 mg L-1 IBA in concentrated solution led to the highest rooting percentage (14 percent), although not significantly different from the remaining treatments. The diluted solution did not show promising results for root system induction due to the high mortality of cuttings. The results for IBA as talc were not higher than those for the best treatments in concentrated solution, which indicates this method is not recommended to increase Sapium glandulatum rooting induction. Thus, sprouting cuttings of the year from this species are not recommended for its vegetative propagation, according to the evaluated treatments.
Subject(s)
Plants, Medicinal/classification , Methods , Euphorbiaceae/classification , Sapium/classification , Boric Acids/analysisABSTRACT
Larvicidal activity of ethyl acetate, butanol, and petroleum ether extracts of five species of Euphorbiaceae plants, Jatropha curcas, Pedilanthus tithymaloides, Phyllanthus amarus, Euphorbia hirta, and Euphorbia tirucalli, were tested against the early fourth instar larvae of Aedes aegypti L. and Culex quinquefasciatus (Say). The larval mortality was observed after 24 h of exposure. All extracts showed low larvicidal effects; however, the highest larval mortality was found in petroleum ether extract. The LC50 value of petroleum ether extracts of J. curcas, P. tithymaloides, P. amarus, E. hirta, and E. tirucalli were 8.79, 55.26, 90.92, 272.36, and 4.25 ppm, respectively, against A. aegypti and 11.34, 76.61, 113.40, 424.94, and 5.52 ppm, respectively, against C quinquefasciatus. Of the various ratios tested, the petroleum ether extracts of J. curcas and E. tirucalli were observed to be more efficient than the other plant extracts. It is, therefore, suggested that E. tirucalli can be applied as an ideal potential larvicide against A. aegypti and C. quinquefasciatus. This is an ideal ecofriendly approach for the control of the dengue vector, A. aegypti, and the lymphatic filariasis vector, C. quinquefasciatus.