ABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE: As reported in the Ancient Chinese Medicinal Books, Ginkgo biloba L. fruit has been used as a traditional Chinese medicine for the treatment asthma and cough or as a disinfectant. Our previous study demonstrated that G. biloba exocarp extract (GBEE), an extract of a traditional Chinese herb, inhibits the formation of methicillin-resistant Staphylococcus aureus (MRSA) biofilms. However, GBEE is a crude extract that contains many components, and the underlying mechanisms of purified GBEE fractions extracted with solvents of different polarities are unknown. AIM OF THE STUDY: This study aimed to investigate the different components in GBEE fractions extracted with solvents of different polarities and their antibacterial effects and mechanisms against MRSA and Staphylococcus haemolyticus biofilms both in vitro and in vivo. METHODS: The components in different fractions were detected by high-performance liquid chromatography-high resolution mass spectrometry (HPLC-HRMS). Microbroth dilution assays and time growth curves were used to determine the antibacterial effects of the fractions on 15 clinical bacterial isolates. Crystal violet staining, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were utilized to identify the fractions that affected bacterial biofilm formation. The potential MRSA targets of the GBEE fraction obtained with petroleum ether (PE), denoted GBEE-PE, were screened by transcriptome sequencing, and the gene expression profile was verified by quantitative polymerase chain reaction (qPCR). RESULTS: HPLC-HRMS analysis revealed that the four GBEE fractions (extracted with petroleum ether, ethyl acetate, n-butanol, and water) contained different ginkgo components, and the antibacterial effects decreased as the polarity of the extraction solvent increased. The antibacterial activity of GBEE-PE was greater than that of the GBEE fraction extracted with ethyl acetate (EA). GBEE-PE improved H. illucens survival and reduced MRSA colonization in model mouse organs. Crystal violet staining and SEM and TEM analyses revealed that GBEE-PE inhibited MRSA and S. haemolyticus biofilm formation. Transcriptional analysis revealed that GBEE-PE inhibits MRSA biofilms by altering ion transport, cell wall metabolism and virulence-related gene expression. In addition, the LO2 cell viability and H. illucens toxicity assay data showed that GBEE-PE at 20 mg/kg was nontoxic. CONCLUSION: The GBEE fractions contained different components, and their antibacterial effects decreased with increases in the polarity of the extraction solvent. GBEE-PE limited MRSA growth and biofilm formation by affecting ion transport, cell wall synthesis, and virulence-related pathways. This research provides a more detailed overview of the mechanism by which GBEE-PE inhibits MRSA both in vitro and in vivo and suggests that GBEE-PE is a new prospective antimicrobial with the potential to be used in MRSA therapeutics in the future.
Subject(s)
Acetates , Alkanes , Methicillin-Resistant Staphylococcus aureus , Animals , Mice , Ginkgo biloba/chemistry , Virulence , Gentian Violet/pharmacology , Prospective Studies , Plant Extracts/pharmacology , Solvents/chemistry , Anti-Bacterial Agents/pharmacology , Biofilms , Microbial Sensitivity TestsABSTRACT
BACKGROUND: Methods like the bio-synthesis of silver nanoparticles (Ag NPs) using plant extracts have become promising due to their eco-friendly approach. The study aimed to examine the utilization of Garcinia gummi-gutta fruit phytochemicals as agents in the biosynthesis of Ag NPs, evaluation of the antimicrobial, antioxidant, and anti-cancerous properties, as well as the photocatalytic ability of bio-synthesized Ag NPs against Crystal Violet (CV), a triphenylmethane dye. METHODS: The characterization of the physical properties of the Ag NPs synthesized via the green route was done using UV-Vis spectrophotometry (UV-Vis), X-ray Diffraction (XRD), Fourier Transform Infrared Spectrophotometry (FTIR), Scanning Electron Microscopy (SEM), Zeta potential analysis, and Transmission Electron Microscopy (TEM). The dye degradation efficiency of CV was determined using synthesized Ag NPs under UV light by analyzing the absorption maximum at 579 nm. The antimicrobial efficacy of Ag NPs against E. coli, S. aureus, Candida tropicalis, and Candida albicans was examined using the broth dilution method. The antioxidant and anti-cancer properties of the synthesized Ag NPs were assessed using the DPPH and MTT assays. RESULTS: The UV analysis revealed that the peak of synthesized Ag NPs was 442 nm. Data from FTIR, XRD, Zeta potential, SEM, and TEM analysis confirmed the formation of nanoparticles. The SEM and TEM analysis identified the presence of spherical nanoparticles with an average size of 29.12 nm and 24.18 nm, respectively. Maximum dye degradation efficiency of CV was observed at 90.08% after 320 min without any silver leaching, confirming the photocatalytic activity of Ag NPs. The bio-efficiency of the treatment was assessed using the Allium cepa root growth inhibition test, toxicity analysis on Vigna radiata, and Brine shrimp lethality assay. CONCLUSIONS: The findings revealed the environmentally friendly nature of green Ag NPs over physical/chemically synthesized Ag NPs. The synthesized Ag NPs can effectively be used in biomedical and photocatalytic applications.
Subject(s)
Anti-Infective Agents , Garcinia , Metal Nanoparticles , Neoplasms , Antioxidants/pharmacology , Silver/pharmacology , Escherichia coli , Staphylococcus aureus , Anti-Infective Agents/pharmacology , Gentian VioletABSTRACT
Healthcare-associated infections can occur and spread through direct contact with contaminated fomites in a hospital, such as mobile phones, tablets, computer keyboards, doorknobs, and other surfaces. Herein, this study shows a transparent, robust, and visible light-activated antibacterial surface based on hydrogen bonds between a transparent silica-alumina (Si-Al) sol-gel and a visible light-activated photosensitizer, such as crystal violet (CV). The study of the bonding mechanisms revealed that hydrogen bonding predominantly occurs between the N of CV and Al-OH. Apart from CV, Si-Al can be combined with a variety of dyes, highlighting its potential for wide application. The Si-Al@CV film selectively generates singlet oxygen using ambient visible light, triggering potent photochemical antibacterial performance against Gram-positive and Gram-negative bacteria. Additionally, the Si-Al@CV film is stable even after mechanical stability tests such as tape adhesion, scratch, bending, and water immersion. In vitro cytotoxicity tests using C2C12 myoblast cells showed that the Si-Al@CV film is a biocompatible material. This work suggests a new approach for designing a transparent and robust touchscreen surface with photochemical antibacterial capability against healthcare-associated infections.
Subject(s)
Aluminum Oxide , Cross Infection , Humans , Silicon Dioxide/pharmacology , Hydrogen Bonding , Coloring Agents , Anti-Bacterial Agents/pharmacology , Gram-Negative Bacteria , Gram-Positive Bacteria , Cations , Gentian Violet/pharmacology , Silica GelABSTRACT
Introduction. Staphylococcus aureus is a major cause of chronic diseases and biofilm formation is a contributing factor. 20S-ginsenoside Rg3 (Rg3) is a natural product extracted from the traditional Chinese medicine red ginseng.Gap statement. The effects of Rg3 on biofilm formation and haemolytic activity as well as its antibacterial mechanism against S. aureus have not been reported.Aim. This study aimed to investigate the effects of Rg3 on biofilm formation and haemolytic activity as well as its antibacterial action against clinical S. aureus isolates.Methodology. The effect of Rg3 on biofilm formation of clinical S. aureus isolates was studied by crystal violet staining. Haemolytic activity analysis was carried out. Furthermore, the influence of Rg3 on the proteome profile of S. aureus was studied by quantitative proteomics to clarify the mechanism underlying its antibacterial action and further verified by reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR).Results. Rg3 significantly inhibited biofilm formation and haemolytic activity in clinical S. aureus isolates. A total of 63 with >1.5-fold changes in expression were identified, including 34 upregulated proteins and 29 downregulated proteins. Based on bioinformatics analysis, the expression of several virulence factors and biofilm-related proteins, containing CopZ, CspA, SasG, SaeR/SaeS two-component system and SaeR/SaeS-regulated proteins, including leukocidin-like protein 2, immunoglobulin-binding protein G (Sbi) and fibrinogen-binding protein, in the S. aureus of the Rg3-treated group was downregulated. RT-qPCR confirmed that Rg3 inhibited the regulation of SaeR/SaeS and decreased the transcriptional levels of the biofilm-related genes CopZ, CspA and SasG.Conclusions. Rg3 reduces the formation of biofilm by reducing cell adhesion and aggregation. Further, Rg3 can inhibit the SaeR/SaeS two-component system, which acts as a crucial signal transduction system for the anti-virulence activity of Rg3 against clinical S. aureus isolates.
Subject(s)
Biological Products , Staphylococcal Infections , Humans , Staphylococcus aureus/genetics , Leukocidins , Gentian Violet/metabolism , Proteome/metabolism , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Transcription Factors/genetics , Virulence Factors/genetics , Virulence Factors/metabolism , Biofilms , Anti-Bacterial Agents/pharmacology , Anti-Bacterial Agents/metabolism , Fibrinogen/metabolism , Immunoglobulins/metabolismABSTRACT
BACKGROUND: Biofilm-related infections are difficult to be treated because of higher resistance to antimicrobial agents. Current study aims to characterize the influence of zinc oxide nanoparticles (ZnO-NPs) on both S. aureus susceptibility to antibiotics and pathogenesis. METHODS: The influence of ZnO-NPs on biofilm formation by S. aureus was characterized by the crystal violet and tube assay. The synergistic effect of ZnO-NPs in combination with antibiotics on S. aureus was characterized using the checkerboard method. The effect of ZnO-NPs on S. aureus cell surface hydrophobicity and blood hemolysis was investigated. RT-qPCR was used to investigate the effect of ZnO-NPs on the expression of biofilm related genes (icaA, icaR and sarA), katA and sigB. The impact of ZnO-NPs on S. aureus pathogenesis was evaluated using mice infection model. RESULTS: ZnO-NPs exhibited a good antibiofilm activity against S. aureus. The findings indicate a synergistic antibiofilm effect of combination between ZnO-NPs and tested antibiotics. ZnO-NPs were capable of decreasing S. aureus cell surface hydrophobicity which could account for observed decrease in bacterial biofilm forming capacity. Moreover, ZnO-NPs-treated bacteria exhibited a significant decrease in blood hemolysis relative to control untreated S. aureus. The expression of biofilm related genes was significantly repressed in ZnO-NPs treated bacteria as compared to untreated cells. Finally, the effect of ZnO-NPs on S. aureus pathogenesis was investigated using mice infection model where ZnO-NPs accelerated healing of wounds in mice as compared to control untreated mice. CONCLUSIONS: Present data support the efficiency of ZnO-NPs as antibiofilm agent in treatment of S. aureus infections. This study recommends the incorporation of ZnO-NPs as adjuvant with other antibiotics targeting S. aureus based on the promising findings obtained herein in order to control infection with this pathogen.
Subject(s)
Metal Nanoparticles , Nanoparticles , Staphylococcal Infections , Zinc Oxide , Animals , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacology , Bacteria/metabolism , Biofilms , Gentian Violet/pharmacology , Hemolysis , Iron-Dextran Complex/pharmacology , Metal Nanoparticles/chemistry , Mice , Microbial Sensitivity Tests , Nanoparticles/chemistry , Staphylococcal Infections/drug therapy , Staphylococcus aureus , Virulence , Zinc Oxide/chemistry , Zinc Oxide/pharmacologyABSTRACT
Data regarding the therapeutic potential of Caladium lindenii (C. lindenii) are insufficient. It becomes more important to explore plants as an alternative or palliative therapeutics in deadly diseases around the globe. The current study was planned to explore C. lindenii for its anticancer activity of ethanolic and hexane extracts of C. lindenii leaves against hepatic carcinoma (HepG2) and human embryonic kidney (HEK293T) cell lines. HepG2 and HEK293T cells were treated with 10, 50, 100, 200, and 400 µg/mL of ethanolic and hexane extracts of C. lindenii and were incubated for 72 h. Antiproliferative activity was measured by 3-(4,5-dimethylthiazol-2yl)-2,5-biphenyl tetrazolium bromide (MTT) assay, and percentage viability were calculated through crystal violet staining and cellular morphology by Floid Cell Imaging Station. The study showed ethanolic extract exhibiting a significantly higher antiproliferative effect on HepG2 (IC50 = 31µg/mL) in a concentration-dependent manner, while HEK293T (IC50 = 241µg/mL) cells showed no toxicity. Hexane extract exhibited lower cytotoxicity (IC50 = 150µg/mL) on HepG2 cells with no effect on HEK293T (IC50 = 550µg/mL). On the other hand, the percentage viability of HepG2 cells was recorded as 78%, 67%, 50%, 37%, and 28% by ethanolic extracts, and 88%, 80%, 69%, 59%, and 50% by hexane extracts at tested concentrations of both extracts. Toxicity assay showed significantly safer ranges of percentage viabilities in normal cells (HEK293T), i.e., 95%, 90%, 88%, 76%, and 61% with ethanolic extract and 97%, 95%, 88%, 75%, and 62% with hexane extract. The assay validity revealed 100% viability in the control negative (dimethyl sulfoxide treated) and less than 45% in the control positive (cisplatin) on both HepG2 and HEK293T cells. Morphological studies showed alterations in HepG2 cells upon exposure to >50 µg/mL of ethanolic extracts and ≥400 µg/mL of hexane extracts. HEK293T on the other hand did not change its morphology against any of the extracts compared to the aggressive changes on the HepG2 cell line by both extracts and positive control (cisplatin). In conclusion, extracts of C. lindenii are proved to have significant potential for cytotoxicity-induced apoptosis in human cancer HepG2 cells and are less toxic to normal HEK293T cells. Hence C. lindenii extracts are proposed to be used against hepatocellular carcinoma (HCC) after further validations.
Subject(s)
Carcinoma, Hepatocellular , Liver Neoplasms , Bromides/therapeutic use , Carcinoma, Hepatocellular/drug therapy , Carcinoma, Hepatocellular/pathology , Cisplatin/therapeutic use , Dimethyl Sulfoxide , Gentian Violet/therapeutic use , HEK293 Cells , Hexanes , Humans , Liver Neoplasms/drug therapy , Liver Neoplasms/pathology , Plant Extracts/chemistryABSTRACT
BACKGROUND: Enterococcus faecalis (E. faecalis) plays an important role in the failure of root canal treatment and refractory periapical periodontitis. As an important virulence factor of E. faecalis, extracellular polysaccharide (EPS) serves as a matrix to wrap bacteria and form biofilms. The homologous rnc gene, encoding Ribonuclease III, has been reported as a regulator of EPS synthesis. In order to develop novel anti-biofilm targets, we investigated the effects of the rnc gene on the biological characteristics of E. faecalis, and compared the biofilm tolerance towards the typical root canal irrigation agents and traditional Chinese medicine fluid Pudilan. METHODS: E. faecalis rnc gene overexpression (rnc+) and low-expression (rnc-) strains were constructed. The growth curves of E. faecalis ATCC29212, rnc+, and rnc- strains were obtained to study the regulatory effect of the rnc gene on E. faecalis. Scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), and crystal violet staining assays were performed to evaluate the morphology and composition of E. faecalis biofilms. Furthermore, the wild-type and mutant biofilms were treated with 5% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), and Pudilan. The residual viabilities of E. faecalis biofilms were evaluated using crystal violet staining and colony counting assays. RESULTS: The results demonstrated that the rnc gene could promote bacterial growth and EPS synthesis, causing the EPS-barren biofilm morphology and low EPS/bacteria ratio. Both the rnc+ and rnc- biofilms showed increased susceptibility to the root canal irrigation agents. The 5% NaOCl group showed the highest biofilm removing effect followed by Pudilan and 2% CHX. The colony counting results showed almost complete removal of bacteria in the 5% NaOCl, 2% CHX, and Chinese medicine agents' groups. CONCLUSIONS: This study concluded that the rnc gene could positively regulate bacterial proliferation, EPS synthesis, and biofilm formation in E. faecalis. The rnc mutation caused an increase in the disinfectant sensitivity of biofilm, indicating a potential anti-biofilm target. In addition, Pudilan exhibited an excellent ability to remove E. faecalis biofilm.
Subject(s)
Disinfectants , Enterococcus faecalis , Chlorhexidine/pharmacology , Disinfection , Enterococcus faecalis/genetics , Gentian Violet/pharmacology , Humans , Ribonuclease III/pharmacology , Sodium Hypochlorite/pharmacology , Virulence Factors/pharmacologyABSTRACT
Objective: The objective of this study is to report on the bactericidal effects of blue light administered at low irradiance for extended periods of time. Background: Multidrug-resistant organisms (MDROs) utilize biofilms that can limit the efficacy of antibiotics, causing infection and impaired wound healing. Unlike high-energy systems, continuous low-irradiance phototherapy (CLIP) avoids thermal injury of healthy tissue and can be delivered for extended periods. Methods: Four MDRO species, two of which contained different antibiotic resistance genes, were exposed to 405 nm irradiation in vitro. The microbes were incrementally exposed to increasing dose-rates (irradiance; mW/cm2) over a 24-h time period. Cell viability and biomass reduction assays were conducted to quantify the antibacterial/antibiofilm effects. Primary human dermal fibroblasts were also exposed to CLIP to assess whether these dose-rates would impair cell viability or proliferation. Results: CLIP exposure utilizing irradiances as low as 2.78 mW/cm2 delivered over 24 h resulted in a >3.0-log (>99.9%) and >2.0-log (>99.0%) microbial load reduction when organisms were grown in planktonic and biofilm-encapsulated conditions, respectively. Crystal violet biofilm assays revealed destruction of extracellular biofilm architecture following CLIP exposure. Human fibroblast viability and proliferation were unaffected by CLIP. Conclusions: This is the first report demonstrating the antimicrobial efficacy of CLIP for MDROs found in infected wounds. CLIP did not compromise cultured human fibroblast growth and survival. This study demonstrated that very low fluence rates (irradiances) delivered over extended periods are potently antimicrobial. There is translational potential for CLIP to be fabricated as a wearable device that would enable continuous ambulatory care of wounds.
Subject(s)
Anti-Infective Agents , Drug Resistance, Multiple, Bacterial , Anti-Bacterial Agents/pharmacology , Anti-Infective Agents/pharmacology , Gentian Violet , Humans , PhototherapyABSTRACT
BACKGROUND: Ovarian cancer has the highest mortality among all gynecological malignancies; currently, no effective therapeutics are available for its treatment. Naringenin has been shown to inhibit the progression of various cancers, but its inhibitory effect on ovarian cancer remains unknown. PURPOSE: This study aimed to evaluate the inhibitory effects of naringenin on ovarian cancer and elucidate the underlying mechanisms. METHODS: Cancer cell proliferation was detected by cell counting kit-8 and crystal violet assays, and the migration capability was determined by wound healing and transwell assays. Western blotting and immunohistochemistry assays were employed to determine the expression levels of the epidermal growth factor receptor, phosphatidylinositol 3-kinase (PI3K) and cyclin D1 in vitro and in vivo, respectively. An ES-2 xenograft nude mouse model was established for the in vivo experiments, and fecal samples were collected for intestinal microbiota analysis by 16S rDNA sequencing. RESULTS: Naringenin suppressed the proliferation and migration of A2780 and ES-2 cancer cell lines and downregulated PI3K in vitro. In animal experiments, naringenin treatment significantly decreased the tumor weight and volume, and oral administration exhibited greater effects than intraperitoneal injection. Additionally, naringenin treatment ameliorated the population composition of the microbiota in animals with ovarian cancer and significantly increased the abundances of Alistipes and Lactobacillus. CONCLUSION: Naringenin suppresses epithelial ovarian cancer by inhibiting PI3K pathway expression and ameliorating the gut microbiota, and the oral route is more effective than parenteral administration.
Subject(s)
Gastrointestinal Microbiome , Ovarian Neoplasms , Animals , Carcinoma, Ovarian Epithelial/drug therapy , Cell Line, Tumor , Cell Movement , Cell Proliferation , Cyclin D1 , DNA, Ribosomal/pharmacology , ErbB Receptors/metabolism , Female , Flavanones , Gentian Violet/pharmacology , Gentian Violet/therapeutic use , Humans , Mice , Mice, Nude , Ovarian Neoplasms/drug therapy , Ovarian Neoplasms/pathology , Phosphatidylinositol 3-Kinase/metabolism , Phosphatidylinositol 3-Kinases/metabolismABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE: The fruit of Ginkgo biloba L. (Ginkgo nuts) has been used for a long time as a critical Chinese medicine material to treat cough and asthma, as well as a disinfectant. Similar records were written in the Compendium of Materia Medica (Ben Cao Gang Mu, pinyin in Chinese) and Sheng Nong's herbal classic (Shen Nong Ben Cao Jing, pinyin in Chinese). Recent research has shown that Ginkgo biloba exocarp extract (GBEE) has the functions of unblocking blood vessels and improving brain function, as well as antitumour activity and antibacterial activity. GBEE was shown to inhibit methicillin-resistant Staphylococcus aureus (MRSA) biofilm formation as a traditional Chinese herb in our previous report in this journal. AIM OF THE STUD: yThe antibiotic resistance of clinical bacteria has recently become increasingly serious. Thus, this study aimed to investigate the Ginkgo biloba exocarp extract (GBEE) antibacterial lineage, as well as its effect and mechanism on S. haemolyticus biofilms. This study will provide a new perspective on clinical multidrug resistant (MDR) treatment with ethnopharmacology herbs. METHODS: The microbroth dilution assay was carried out to measure the antibacterial effect of GBEE on 13 types of clinical bacteria. Bacterial growth curves with or without GBEE treatment were drawn at different time points. The potential targets of GBEE against S. haemolyticus were screened by transcriptome sequencing. The effects of GBEE on bacterial biofilm formation and mature biofilm disruption were determined by crystal violet staining and scanning electron microscopy. The metabolic activity of bacteria inside the biofilm was assessed by colony-forming unit (CFU) counting and (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2HY-tetrazolium bromide (MTT) assay. Quantitative polymerase chain reaction (qPCR) was used to measure the gene expression profile of GBEE on S. haemolyticus biofilm-related factors. RESULTS: The results showed that GBEE has bacteriostatic effects on 3 g-positive (G+) and 2 g-negative (G-) bacteria among 13 species of clinical bacteria. The antibacterial effect of GBEE supernatant liquid was stronger than the antibacterial effect of GBEE supernviaould-like liquid. GBEE supernatant liquid inhibited the growth of S. epidermidis, S. haemolyticus, and E. faecium at shallow concentrations with minimum inhibitory concentrations (MICs) of 2 µg/ml, 4 µg/ml and 8 µg/ml, respectively. Genes involved in quorum sensing, two-component systems, folate biosynthesis, and ATP-binding cassette (ABC) transporters were differentially expressed in GBEE-treated groups compared with controls. Crystal violet, scanning electron microscopy (SEM) and MTT assays showed that GBEE suppressed S. haemolyticus biofilm formation in a dose-dependent manner. Moreover, GBEE supernatant liquid downregulated cidA, cidB and atl, which are involved in cell lysis and extracellular DNA (eDNA) release, as well as downregulated the cbp, ebp and fbp participation in encoding cell-surface binding proteins. CONCLUSIONS: GBEE has an excellent antibacterial effect on gram-positive bacteria and also inhibits the growth of gram-negative bacteria, such as A. baumannii (carbapenem-resistant Acinetobacter baumannii) CRABA and S. maltophilia. GBEE inhibits the biofilm formation of S. haemolyticus by altering the regulation and biofilm material-related genes, including the release of eDNA and cell-surface binding proteins.
Subject(s)
Methicillin-Resistant Staphylococcus aureus , Staphylococcus haemolyticus , Anti-Bacterial Agents/pharmacology , Bacteria , Biofilms , Gentian Violet/pharmacology , Ginkgo biloba/chemistry , Microbial Sensitivity Tests , Plant Extracts/pharmacologyABSTRACT
The purpose of this study was to develop a chewing gum containing a novel antimicrobial peptide GH12 and evaluate its biocompatibility, antimicrobial activity, and caries-preventive effects in vivo and in vitro. GH12 chewing gum was developed using a conventional method and its extracts were prepared in artificial saliva. GH12 concentration in the extracts was determined by high-performance liquid chromatography; extracts were used for growth curve assay, time-kill assay, crystal violet staining assay, scanning electron microscopy, and Cell Counting Kit-8 assay. A rat caries model was established, and molars were treated topically with extracts for 5 weeks. Weight gain monitoring, hematoxylin-eosin staining, micro-computed tomography, and Keyes scoring were conducted. Significant inhibition of Streptococcus mutans growth and biofilm formation was observed. Extracts displayed low cytotoxicity against human gingival epithelial cells. No significant differences in weight gain or signs of harm to the mucosal tissues in any of the rats were observed. Keyes scores of caries lesions in the GH12 chewing gum group were lower than those of the negative control group. It was concluded that GH12 chewing gum showed good biocompatibility, antimicrobial activity, and caries-preventive effects, exhibiting great potential to prevent dental caries as an adjuvant to regular oral hygiene.
Subject(s)
Anti-Infective Agents , Dental Caries , Animals , Anti-Infective Agents/pharmacology , Antimicrobial Peptides , Chewing Gum/analysis , Dental Caries/prevention & control , Dental Caries Susceptibility , Eosine Yellowish-(YS)/pharmacology , Gentian Violet/pharmacology , Hematoxylin/pharmacology , Humans , Rats , Saliva, Artificial/pharmacology , Streptococcus mutans , Weight Gain , X-Ray MicrotomographyABSTRACT
Green fabrication of copper nanoparticles (CuNPs) is an environmentally friendly and cost-effective method of synthesis for biomedical and bioremediation applications. In recent times, bacterial pathogens contaminating or affecting food and food crops pose the greatest threat to the food industry. In addition to this issue, synthetic dyes released from the textile and dyeing industries are polluting aquatic ecosystems and agricultural lands. The combined impact of these two factors is considered a major threat to life. Therefore, the use of CuNPs will provide an effective and long-term solution as an antibacterial and dye removing agent. The current study focuses on the synthesis of CuNPs using the leaf extract of Chloroxylon swietenia (C-CuNPs). The formation of a peak at 390 nm and a change in color from yellow to dark brown confirmed the synthesis of C-CuNPs. Subsequent synthesis at pH 9 was suitable for preparing C-CuNPs. Structural and chemical characterization of C-CuNPs was performed using Fourier Transfer Infra-Red (FTIR), X-ray diffraction (XRD), Dynamic Light scattering (DLS), and Scanning Electron Microscopy (SEM) analysis. The synthesized C-CuNPs possess a crystalline nature, a functional group that resembles C. swietenia, and are negatively charged and spherical in shape. C-CuNPs were tested against Congo red, Coomassie blue, and crystal violet and they showed complete degradation within 24 h under optimum conditions. Disk diffusion and broth dilution assay were used to test the antibacterial activity of C-CuNPs against Staphylococcus nepalensis, Staphylococcus gallinarum, Pseudomonasstutzeri,Bacillus subtilis, and Enterococcus faecalis. Therefore, the present study represents the first report on C-CuNPs' ability to degrade synthetic dyes and kill foodborne bacterial pathogens. Thus, the study has shed light on the potential of green synthesized CuNPs as bioremediation and packaging material in the future.
Subject(s)
Anti-Infective Agents , Metal Nanoparticles , Rutaceae , Anti-Bacterial Agents/chemistry , Anti-Infective Agents/chemistry , Anti-Infective Agents/pharmacology , Coloring Agents , Congo Red , Copper/chemistry , Copper/pharmacology , Ecosystem , Gentian Violet , Green Chemistry Technology , Metal Nanoparticles/chemistry , Plant Extracts/chemistry , Spectroscopy, Fourier Transform InfraredABSTRACT
Dental caries has been the most widespread chronic disease globally associated with significant health and financial burdens. Caries typically starts in the enamel, which is a unique tissue that cannot be healed or regrown; nonetheless, new preventive approaches have limitations and no effective care has developed yet. Since enamel is a non-renewable tissue, we believe that the intimate overlaying layer, the acquired enamel pellicle (AEP), plays a crucial lifetime protective role and could be employed to control bacterial adhesion and dental plaque succession. Based on our identified AEP whole proteome/peptidome, we investigated the bioinhibitory capacities of the native abundant proteins/peptides adsorbed in pellicle-mimicking conditions. Further, we designed novel hybrid constructs comprising antifouling and antimicrobial functional domains derived from statherin and histatin families, respectively, to attain synergistic preventive effects. Three novel constructs demonstrated significant multifaceted bio-inhibition compared to either the whole saliva and/or its native proteins/peptides via reducing biomass fouling and inducing biofilm dispersion beside triggering bacterial cell death. These data are valuable to bioengineer precision-guided enamel pellicles as an efficient and versatile prevention remedy. In conclusion, integrating complementary acting functional domains of salivary proteins/peptides is a novel translational approach to design multifunctional customizable enamel pellicles for caries prevention.
Subject(s)
Biomimetics , Dental Caries/prevention & control , Peptides/chemistry , Proteins/chemistry , Saliva/metabolism , Adult , Biofilms , Biomass , Dental Caries/microbiology , Dental Enamel/chemistry , Dental Enamel/diagnostic imaging , Durapatite/chemistry , Fluorescence , Gentian Violet , Humans , Imaging, Three-Dimensional , Immobilized Proteins/chemistry , Microbial Sensitivity Tests , Streptococcus mutans/drug effectsABSTRACT
The increasingly common remedial application of nanoscale zero-valent iron (nZVI) to alleviate specific contaminant issues may inadvertently lead to nZVI accumulation in wastewater. This is a potential concern, because the effect of nZVI on the common microbes essential for wastewater biotreatment is not known. This is further complicated when there are many ways available to synthesize nZVI, which may interreact with bacteria differently. Thus, in this study, the different effects of nZVI synthesized by Eucalyptus leaves (EL-nZVI) and a commercially synthesized nZVI on the biodegradation of crystal violet by Burkholderia vietnamiensis C09V (B.V. C09V) was studied. At high dose (1000 mg/L), EL-nZVI and commercial nZVI both significantly inhibited the removal of crystal violet by B.V. C09V, decreasing removal rates by 10.5 and 13.1% respectively. Optical density (OD600) and soluble protein assays indicated that the growth of B.V. C09V improved under low doses (100 mg/L), but remained inhibited under high doses (500 and 1000 mg/L) of both commercial and EL-nZVI. Enzymes were also sensitive to nZVI, where the commercial variant exerted a greater effect on both the activity of lactate dehydrogenase (LDH) and superoxide dismutase (SOD) than EL-nZVI, indicating that EL-nZVI was less toxic than commercial nZVI. LIVE/DEAD staining also showed that the number of apoptotic cells was significantly higher when exposed to commercial nZVI rather than EL-nZVI. Furthermore, scanning electron microscopy (SEM) confirmed that direct contact between nZVI and cells at 1000 mg/L nZVI caused cell membrane disruption. Whereas, at 100 mg/L EL-nZVI, B.V. C09V grew better due to the formation of dense biofilms around the suspended EL-nZVI at a. Fourier transform infrared spectra (FTIR), confirmed an abundance of oxygen-containing functional groups on the surface of EL-nZVI which provided better biocompatibility than commercial nZVI. Overall, while dose was the most significant factor influencing nZVI toxicity, surface composition and morphology was also important. These new findings suggest chemical synthesis of metal nanoparticles should be replaced by biosynthetic routes to maintain viable microbial pollution during wastewater treatment.
Subject(s)
Burkholderia , Water Pollutants, Chemical , Water Purification , Gentian Violet , Iron , Water Pollutants, Chemical/analysis , Water Pollutants, Chemical/toxicityABSTRACT
A new alternative aerogel was prepared from low-cost chitin and psyllium biopolymers to adsorb crystal violet (CV) dye from liquid media and possibly treat effluents containing other dyes. The aerogel was characterized by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM), which demonstrated that aerogel has a typical structure of amorphous materials and presented a randomly interconnected porous structure that resembles an open pore network. 2.5 g L-1 of aerogel was able to remove 86.00% of CV from solutions, and the natural pH of the CV solution was considered the more adequate for adsorption. The pseudo-second-order (PSO) model satisfactorily described the adsorption kinetics, and the Freundlich model was suitable to represent the adsorption equilibrium. The maximum experimental capacity achieved was 227.11 mg g-1, which indicates that aerogel is very efficient and competitive with several adsorbents. Tests using a simulated effluent showed that aerogel has excellent potential to treat real colored effluents.
Subject(s)
Chitin/chemistry , Coloring Agents/chemistry , Gentian Violet/isolation & purification , Psyllium/chemistry , Water Pollutants, Chemical/isolation & purification , Water Purification/methods , Adsorption , Kinetics , Porosity , Thermodynamics , Water/chemistryABSTRACT
PURPOSE: In postoperative peritonitis, Gram stain examination (GSE) of peritoneal fluid has been proposed as a guide for the prescription of glycopeptides and antifungal therapy in empirical antibiotherapy. No data support this approach for Gram-positive cocci. We aimed to evaluate the performance of GSE in predicting the results of the culture of peritoneal fluid. METHODS: In this retrospective single-center study, concordance between GSE and culture of peritoneal fluid was assessed for different types of microorganisms. Factors associated with concordance of the two tests were evaluated in the subpopulation of Gram-positive cocci peritonitis. RESULTS: Among the 152 episodes, the GSE was negative in 57 cases. The negative predictive value and the positive predictive value were 41% and 87% for Gram-positive cocci (GPC), 31% and 86% for Gram-negative bacilli, and 78% and 94% for fungi. GSE is not a reliable guide for the choice of empirical antibiotherapy and cannot reliably rule out the presence of GPC at culture. If we aim to achieve a high rate of adequacy, the systematic use of glycopeptide in the empirical antibiotherapy may be considered. CONCLUSION: GSE shows poor performance to predict the results of culture of peritoneal fluid in postoperative peritonitis. Avoiding covering resistant GPC cannot be based on the result of GSE.
Subject(s)
Anti-Bacterial Agents/therapeutic use , Gentian Violet , Peritonitis/drug therapy , Peritonitis/microbiology , Phenazines , Postoperative Complications/drug therapy , Postoperative Complications/microbiology , Antibiotic Prophylaxis , Female , Humans , Intensive Care Units , Male , Microbial Sensitivity Tests , Middle Aged , Paris , Reoperation , Retrospective StudiesSubject(s)
Blood Culture/statistics & numerical data , Clinical Laboratory Services/organization & administration , Delivery of Health Care, Integrated/organization & administration , Quality Assurance, Health Care/methods , Telecommunications , Blood Culture/methods , Clinical Laboratory Services/statistics & numerical data , Delivery of Health Care, Integrated/statistics & numerical data , Feasibility Studies , Gentian Violet , Humans , Microscopy , Observer Variation , Phenazines , Quality Indicators, Health Care/statistics & numerical data , Staining and Labeling/methods , Staining and Labeling/statistics & numerical dataABSTRACT
INTRODUCTION: Comprehensive wound management programs that employ a standardized integrated care bundle (ICB) and advanced wound dressings are generally recognized to decrease healing times and treatment costs. The purpose of this study was to compare wound healing rates and cost efficiencies as measured by nursing-care requirements for patients not on an ICB versus patients on an ICB and using a gentian violet/methylene blue-impregnated (GV/MB) antimicrobial advanced wound dressing. MATERIALS AND METHODS: The comprehensive wound management programs enabled continuous, standardized measurement of each patient's wound episode from admission with a wound to healing and discharge. Data was recorded over 24 months from 2016 to 2018. The variables recorded for each patient included: wound healing time (number of weeks), wound acuity based on the Bates-Jensen Wound Assessment Tool (BWAT), a comorbidity index (using the Charlson Comorbidity Index), and the number of wound dressing changes. The wound dressing changes required a visit by a registered nurse and, therefore, served as an indicator of care delivery costs where the dressing change visit cost was $68 (CAD). RESULTS: A total of 6300 patients (25% of the total study population) were identified as using GV/MB dressings within the context of an ICB. The mean healing time for these patients was accelerated more than 50% versus patients not on an ICB. The average total cost of patient care was reduced by more than 75% from diagnosis to wound healing when patients were on an ICB with GV/MB dressings. These results compared well to patients on ICBs that had other types of advanced dressings. CONCLUSION: The study demonstrates that a comprehensive wound management program based on integrated care bundles in conjunction with GV/MB dressings can be a highly-effective clinical option. The benefits showed significant reductions in healing times and treatment costs.
Subject(s)
Anti-Infective Agents, Local/administration & dosage , Bandages , Gentian Violet/administration & dosage , Methylene Blue/administration & dosage , Wound Healing , Wounds and Injuries/therapy , Bandages/economics , Bandages/standards , Chronic Disease , Delivery of Health Care, Integrated/economics , Delivery of Health Care, Integrated/standards , Humans , Patient Care Management/economics , Patient Care Management/standards , Quality of Health Care , Retrospective StudiesABSTRACT
Grapefruit seed extract (GSE) is a safe and effective preservative that is used widely in the food industry. However, there are few studies addressing the anti-biofilm effect of GSE. In this study, the anti-biofilm effect of GSE was investigated against biofilm-forming strains of Staphylococcus aureus and Escherichia coli. The GSE minimum inhibitory concentration (MIC) for S. aureus and E. coli were 25 µg/ml and 250 µg/ml, respectively. To investigate biofilm inhibition and degradation effect, crystal violet assay and stainless steel were used. Biofilm formation rates of four strains (S. aureus 7, S. aureus 8, E. coli ATCC 25922, and E. coli O157:H4 FRIK 125) were 55.8%, 70.2%, 55.4%, and 20.6% at 1/2 × MIC of GSE, respectively. The degradation effect of GSE on biofilms attached to stainless steel coupons was observed (≥ 1 log CFU/coupon) after exposure to concentrations above the MIC for all strains and 1/2 × MIC for S. aureus 7. In addition, the specific mechanisms of this anti-biofilm effect were investigated by evaluating hydrophobicity, auto-aggregation, exopolysaccharide (EPS) production rate, and motility. Significant changes in EPS production rate and motility were observed in both S. aureus and E. coli in the presence of GSE, while changes in hydrophobicity were observed only in E. coli. No relationship was seen between auto-aggregation and biofilm formation. Therefore, our results suggest that GSE might be used as an anti-biofilm agent that is effective against S. aureus and E. coli.
Subject(s)
Biofilms/drug effects , Citrus paradisi/chemistry , Escherichia coli/drug effects , Plant Extracts/pharmacology , Seeds/chemistry , Staphylococcus aureus/drug effects , Biofilms/growth & development , Gentian Violet , Hydrophobic and Hydrophilic Interactions , Microbial Sensitivity Tests , Polysaccharides, Bacterial/metabolism , Stainless SteelABSTRACT
Treatment trials of antibiotics for Neisseria gonorrhoeae infections frequently enroll primarily men with urethritis, as the diagnosis of acute gonococcal infection in men with urethritis is easily made by Gram stain of the urethral exudate, followed by confirmatory culture or nucleic acid amplification tests (NAATs). Enrolling women in treatment trials is of great importance, but N. gonorrhoeae cervical infections cause nonspecific symptoms. This makes it difficult to conduct interventional trials, as large numbers of women with nonspecific symptoms need to be screened for infection. Gram stain of cervical secretions has a strikingly low sensitivity, and culture and/or NAAT results are not available at the time of screening. This necessitates recall and delayed treatment of infected women who may not return and who may spread the infection during the interval. In this chapter we present an algorithm, derived from a comparison of women who did, or did not, become infected during exposure, which identifies those women who are highly likely to be infected before culture and/or NAAT results are available. The algorithm provides an efficient way to conduct interventional trials in women without the problem of recall and delayed treatment.