ABSTRACT
Lavandula angustifolia Mill. (lavender) is one of the most used medicinal plants. Herein, we chemically characterised and investigated the antioxidant properties and the capability to inhibit key enzymes for the treatment of type 2 diabetes (TD2) and obesity such as pancreatic lipase, α-glucosidase, and α-amylase of the ethanolic extract of two lavender samples (La1 and La2) from southern Italy. Both extracts significantly inhibited α-glucosidase, while La1 inhibited α-amylase and lipase more effectively than La2. To investigate whether these properties could be due to a direct interaction of the main constituents of the extracts with the targeted enzymes, molecular docking studies have been performed. As a result, the selected compounds were able to interact with the key residues of the binding site of the three proteins, thus supporting biological data. Current findings indicate the new potential of lavender ethanolic extract for the development of novel agents for T2D and obesity.
Subject(s)
Diabetes Mellitus, Type 2 , Lamiaceae , Lavandula , Diabetes Mellitus, Type 2/drug therapy , Lavandula/chemistry , Lavandula/metabolism , Molecular Docking Simulation , alpha-Glucosidases/metabolism , Lamiaceae/metabolism , Ethanol , Plant Extracts/pharmacology , Plant Extracts/chemistry , Antioxidants/pharmacology , alpha-Amylases , Lipase , ObesityABSTRACT
Inflammation is closely related to asthma and its defining feature: airway remodeling. The aim of this study was to determine the effects of extracts of normal (NR) and transformed (TR) Leonotis nepetifolia roots on respiratory cells and against the gingival epithelium. Extracts from NR and TR roots were added to lung fibroblast, bronchial epithelial and gingival fibroblast cell lines, in the presence of HRV-16 infection, to determine their impact on inflammation. The expression of inflammatory cytokines (IL-6, IL-1ß, GM-CSF and MCAF) as well as total thiol contents were assessed. The TR extract inhibited rhinovirus-induced IL-6 and IL-1ß expression in all tested airway cells (p < 0.05). Additionally, the extract decreased GM-CSF expression in bronchial epithelial cells. The tested extracts had positive effects on total thiol content in all tested cell lines. The TR root extract demonstrated wound healing potential. While both tested extracts exhibited anti-inflammatory and antioxidative effects, they were stronger for the TR extract, possibly due to higher concentrations of beneficial metabolites such as phenols and flavonoids. Additionally, wound healing activity was demonstrated for the TR root extract. These results suggest that TR root extract may become a promising therapeutic agent in the future.
Subject(s)
Cytokines , Lamiaceae , Humans , Cytokines/metabolism , Granulocyte-Macrophage Colony-Stimulating Factor , Interleukin-6 , Plant Extracts/pharmacology , Plant Extracts/therapeutic use , Lamiaceae/metabolism , Inflammation/drug therapyABSTRACT
Lepechinia meyenii is a medicinal plant specialized in the biosynthesis of different types of antioxidants including the diterpenes carnosic (CA) acid and carnosol (CS). Herein we present the results of plant tissue culture approaches performed in this medicinal plant with particular emphasis on the generation and evaluation of a cell suspension system for CA and CS production. The effect of sucrose concentration, temperature, pH, and UV-light exposure was explored. In addition, diverse concentrations of microbial elicitors (salicylic acid, pyocyanin, Glucanex, and chitin), simulators of abiotic elicitors (polyethylene glycol and NaCl), and biosynthetic precursors (mevalonolactone, geranylgeraniol, and miltiradiene/abietatriene) were evaluated on batch cultures for 20â days. Miltiradiene/abietatriene obtainment was achieved through a metabolic engineering approach using a recombinant strain of Saccharomyces cerevisiae. Our results suggested that the maximum accumulation (Accmax ) of CA and CS was mainly conferred to stimuli associated with oxidative stress such as UV-light exposure (Accmax , 6.2â mg L-1 ) polyethylene glycol (Accmax , 6.5â mg L-1 ) NaCl (Accmax , 5.9â mg L-1 ) which simulated drought and saline stress, respectively. Nevertheless the bacterial elicitor pyocyanin was also effective to increase the production of both diterpenes (Accmax , 6.4â mg L-1 ). Outstandingly, the incorporation of upstream biosynthetic precursors such as geranylgeraniol and miltiradiene/abietatriene, generated the best results with Accmax of 8.6 and 16.7â mg L-1 , respectively. Optimized batch cultures containing 100â mg L-1 geranylgeraniol, 50â mg L-1 miltiradiene/abietatriene (95 : 5 %) and 5â g L-1 polyethylene glycol treated with 6â min UV light pulse during 30â days resulted in Accmax of 26.7â mg L-1 for CA and 17.3â mg L-1 for CS on daysâ 18-24. This strategy allowed to increase seven folds the amounts of CA and CS in comparison with batch cultures without elicitation (Accmax , 4.3â mg L-1 ).
Subject(s)
Diterpenes , Lamiaceae , Plants, Medicinal , Pyocyanine , Sodium Chloride , Suspensions , Diterpenes/metabolism , Lamiaceae/metabolism , Plants, Medicinal/metabolismABSTRACT
Improving yield and secondary metabolites production of medicinal plants through nutrition management recently has been considered. The present study was done to determine the effects of different ammonium (NH4+) to nitrate (NO3-) ratios (100:0, 75:25, 50:50, 25:75, 0:100) on morphophysiological, nutrient contents (N, P, K, Ca, and Mg), phenolic compounds (Total phenolics (TPC) and flavonoid (TFC) contents and individual phenolics including chlorogenic acid, rosmarinic acid, gallic acid, cinnamic acid, caffeic acid, rutin, p-Coumaric acid, apigenin, and quercetin by HPLC-DAD), essential oil composition (by GC and GC-MS), and antioxidant capacity (by DPPH and FRAP assays) of Moldavian balm (Dracocephalum moldavica L.) in deep water culture (DWC) system. The highest biomass and morphological traits values of D. moldavica observed in 0:100 ratio of NH4+:NO3-. Also, the highest TPC and TFC was earned in plants that supplied with 0:100 ratio of NH4+:NO3-. Using the 25:75 ratio of NH4+:NO3- caused the highest nutrient contents (N, Ca and Mg) in the leaves. p-Coumaric acid was detected as the major abundant phenolic compound in extracts and the application of 75:25 ratio of NH4+:NO3 resulted in the highest amounts of p-Coumaric acid, gallic acid, rosmarinic acid, caffeic acid, quercetin, and rutin. The highest antioxidant capacity by both FRAP and DPPH assays was obtained in 75:25 ratio of NH4+:NO3-. Also, the highest geranial and geranyl acetate, geraniol, and neral were obtained in 75:25, 25:75, and 50:50 ratios of NH4+:NO3-, respectively. Plants supplied with the 0:100 ratio of NH4+:NO3-, had the highest total carotenoids, while the highest chlorophyll a and b content gained with 75:25 ratio of NH4+:NO3-. These results suggest that the management of N source in nutrient recipe could contribute to enhance of morphophysiological traits, antioxidant activity and phytochemical compounds in Moldavian balm.
Subject(s)
Ammonium Compounds , Antipsychotic Agents , Lamiaceae , Oils, Volatile , Ammonium Compounds/metabolism , Antioxidants/analysis , Antioxidants/pharmacology , Apigenin , Caffeic Acids , Carotenoids , Chlorogenic Acid , Chlorophyll A , Cinnamates , Coumaric Acids , Depsides , Flavonoids/pharmacology , Gallic Acid , Lamiaceae/metabolism , Nitrates/metabolism , Phytochemicals/pharmacology , Plant Extracts/pharmacology , Quercetin , Rutin , Water , Rosmarinic AcidABSTRACT
Rydingia michauxii (Briq.) Scheen and V.A.Albert (Lamiaceae) is used in Iranian traditional medicine to treat malaria, diabetes, hyperlipidemia, rheumatism and cardiovascular diseases. NMR and LC-DAD-MSn analyses were used to establish extract composition and phenylethanoid, flavonoid glycosides, lignans, labdane diterpenes and iridoids were identified and quantified. The main constituents were isolated, and structures were elucidated based on NMR, polarimetric and MS measurements. A new natural compound, ent-labda-8(17),13-dien-18-glucopyranosyl ester-15,16-olide is described here. The effects of ent-labda-8(17),13-dien-18-oic acid-15,16-olide (1), ent-labda-8(17),13-dien-18-glucopyranosyl es-ter-15,16-olide (2), antirrhinoside (3), echinacoside (4), verbascoside (5), and apigenin 6,8-di-C-glucoside (6), on the low-density lipoprotein receptor (LDLR) and proprotein convertase subtilisin/kexin type 9 (PCSK9), were studied in the human hepatocarcinoma cell line Huh7. Among the six constituents, (3) showed the strongest induction of the LDLR (3.7 ± 2.2 fold vs. control) and PCSK9 (3.2 ± 1.5 fold vs. control) at a concentration of 50 µM. The in vitro observations indicated a potential lipid lowering activity of (3) with a statin-like mechanism of action.
Subject(s)
Biological Products , Lamiaceae , Liver Neoplasms , Biological Products/pharmacology , Chromatography, Liquid , Humans , Iran , Lamiaceae/metabolism , Plant Extracts/pharmacology , Proprotein Convertase 9/metabolism , Receptors, LDL/metabolism , Tandem Mass SpectrometryABSTRACT
The phytoremediation potential and enzymatic defense of a medicinal herb Leucas aspera was studied in the crude oil contaminated soil. The productivity, antioxidants, and phytochemical and functional group profiles of the plant species in stress conditions were investigated. Besides, changes in enzymes, beneficial bacterial population, and physico-chemical and total oil and grease (TOG) profiles in the contaminated soil were also studied. The results showed improvement in physico-chemical conditions, increase in beneficial bacterial population (4.1-5.4 folds), and decrease in TOG (31.3%) level of the contaminated soil by end of the experimental trials. The L. aspera treated contaminated soil showed enhancement in dehydrogenase (32.3%), urease (102.8%), alkaline phosphatase (174.4%), catalase (68.5%), amylase (76.16%), and cellulase (23.6%) activities by end of the experimental trials. Furthermore, there were significant variations in leaf area index, chlorophyll, and biomass contents of the experimental plant as against the initial level and control. Besides, the significant reduction in IC50 values (24-27.4%) of L. aspera samples grown in contaminated soil confirms the strong antioxidant enzymatic defense of the plant species against the crude oil associated abiotic stress. The Fourier-transform infrared (FT-IR) analysis confirmed the uptake and metabolism of aliphatic hydrocarbons, aldehydes, alkyl halides, and nitro compounds by the experimental plant from the contaminated soil.
Subject(s)
Lamiaceae , Petroleum , Soil Pollutants , Antioxidants/metabolism , Biodegradation, Environmental , Hydrocarbons/analysis , Lamiaceae/metabolism , Petroleum/analysis , Plants/metabolism , Soil/chemistry , Soil Pollutants/analysis , Spectroscopy, Fourier Transform InfraredABSTRACT
Clinopidium vulgare L. (wild basil, Lamiaceae) is a well-known medicinal plant used in the traditional medicine in many countries. Medicinal plants present potential sources of bioactive compounds. Many of them are rich in polyphenol compounds that show biological potential in terms of protecting biological molecules from oxidation and in inhibition of cholinesterase enzymes, which may be significant in the treatment of diseases related to oxidative stress. In this work, we examined the chemical composition of Clinopodium vulgare L. hot water and methanol extract using spectroscopic and HPLC/DAD techniques. Using DPPH and FRAP methods the antioxidant activity was analyzed. The ability to protect proteins and lipids from oxidation was also determined as well as the ability of extracts to inhibit cholinesterase enzymes using Ellman's method. Analyzed extracts were rich in polyphenol compounds. Among 16 identified and quantified phenolic compounds dominant were: rosmarinic (26.63 and 34.21â mg/g) and ellagic acid (23.11 and 29.31â mg/g) of hot water and methanol extract, respectively. They show good antioxidant activity and good potential in protecting lipids from oxidation. The ability of extracts to inhibit enzyme acetylcholinesterase was weak, while inhibition of the butyrylcholinesterase was missing. Extracts show prooxidant activity in terms of protecting proteins from oxidation.
Subject(s)
Lamiaceae , Plants, Medicinal , Acetylcholinesterase/metabolism , Antioxidants/chemistry , Butyrylcholinesterase/metabolism , Cholinesterase Inhibitors/chemistry , Cholinesterase Inhibitors/pharmacology , Flavonoids/chemistry , Lamiaceae/metabolism , Lipids , Methanol , Plant Extracts/chemistry , Plant Extracts/pharmacology , Plants, Medicinal/metabolism , Polyphenols/pharmacology , WaterABSTRACT
Monoterpenes make up the largest group of plant secondary metabolites. They can be found in numerous plants, among others, the Lamiaceae family. The compounds demonstrate antioxidative, antibacterial, sedative and anti-inflammatory activity, hence, they are often employed in medicine and pharmaceuticals. Additionally, their fragrant character is often made use of, notably in the food and cosmetic industries. Nevertheless, long-lasting studies have revealed their toxic properties. This fact has led to a detailed analysis of the compounds towards their side effects on the human organism. Although most are safe for human food and medical applications, there are monoterpene compounds that, in certain amounts or under particular circumstances (e.g., pregnancy), can cause serious disorders. The presented review characterises in vitro and in vivo, the toxic character of selected monoterpenes (α-terpinene, camphor, citral, limonene, pulegone, thujone), as well as that of their original plant sources and their essential oils. The selected monoterpenes reveal various toxic properties among which are embryotoxic, neurotoxic, allergenic and genotoxic. It is also known that the essential oils of popular plants can also reveal toxic characteristics that many people are unaware of.
Subject(s)
Lamiaceae , Oils, Volatile , Camphor , Female , Humans , Lamiaceae/metabolism , Limonene/metabolism , Monoterpenes/metabolism , Monoterpenes/toxicity , Oils, Volatile/metabolism , Oils, Volatile/toxicity , Plant Oils/metabolism , PregnancyABSTRACT
Hymenocrater longiflorus (surahalala) is a wild plant species with potential pharmaceutical and ornamental interest. To date, the genomics of this plant is unknown and the gene expression profiling of the genes related to its metabolite has never been studied before. In order to study the responses of in vitro-grown surahalala plants to abiotic stresses and the differential expression of the genes related to its essential oils under exogenous proline application; three levels of PEG600 (0, 10, and 20%) and five levels of proline (0, 5, 10, 15, and 20 µm) were combined in the culture media. Thus, water deficit increased oxidants levels and decreased fresh weight of surahalala tissues, whereas addition of proline up to 15 µm was able to relatively compensate the negative effect of water deficit. Contrarily, high proline level (20 µm) had a negative effect on surahalala plants probably due to the stress simulation (nutrition) under high proline concentration. In addition, the best combination for achieving highest essential oils content was 10 µm proline plus 10% PEG. The expressional profiling of the genes TPS27, L3H, TPS2, TPS1, OMT and GDH3 were successfully carried out and their involvement in 1,8-cineole, carvone, α-pinene, thymol, estragole and ß-Citronellol biosynthesis, respectively, was verified. In addition, our results indicated that these genes could also be involved in the synthesis of other metabolites under water deficit condition.
Subject(s)
Lamiaceae , Oils, Volatile , Plants, Medicinal , Gene Expression Profiling , Gene Expression Regulation, Plant , Lamiaceae/genetics , Lamiaceae/metabolism , Monoterpenes , Plants, Medicinal/metabolism , Proline/metabolism , Stress, Physiological , Water/metabolismABSTRACT
Tanshinones are the bioactive diterpenoid constituents of the traditional Chinese medicinal herb Danshen (Salvia miltiorrhiza), and are examples of the phenolic abietanes widely found within the Lamiaceae plant family. Due to the significant interest in these labdane-related diterpenoid natural products, their biosynthesis has been intensively investigated. In addition to providing the basis for metabolic engineering efforts, this work further yielded pioneering insights into labdane-related diterpenoid biosynthesis in the Lamiaceae more broadly. This includes stereochemical foreshadowing of aromatization, with novel protein domain loss in the relevant diterpene synthase, as well as broader phylogenetic conservation of the relevant enzymes. Beyond such summary of more widespread metabolism, formation of the furan ring that characterizes the tanshinones also has been recently elucidated. Nevertheless, the biocatalysts for the pair of demethylations remain unknown, and the intriguing potential connection of these reactions to the further aromatization observed in the tanshinones are speculated upon here.
Subject(s)
Diterpenes , Lamiaceae , Salvia miltiorrhiza , Abietanes/chemistry , Abietanes/metabolism , Diterpenes/metabolism , Lamiaceae/metabolism , Phylogeny , Salvia miltiorrhiza/chemistry , Salvia miltiorrhiza/metabolismABSTRACT
The presence of intricate carbon skeletons in natural compounds enhances their bioactivity spectrum with unique modes of action at several targets in various dreadful diseases like cancer. The present study was designed to purify the molecules from Thymus linearis and elucidate their antiproliferative activity. The compounds were isolated from the active methanolic extract of Thymus linearis through column chromatography and characterized by various spectroscopic techniques. Antiproliferative activity of isolated compounds was evaluated using MTT assay on cancer and normal cell lines. Mechanism of cell death was elucidated using flow cytometric, microscopic, and Western blot analysis. Four compounds, Sitosterol, Chrysin, 3ß-hydroxylup-12-en-28-oic acid (3BH), and ß-Sitosterol glycoside, were isolated. Among these, 3BH was most potent antiproliferative agent across all cell lines under study, HCT-116 being the most affected one. 3BH was demonstrated to downregulate PI3Ksubunits (p110α and p85α), downstream pAktSer473 and prompted G1 phase cell cycle arrest. The cell cycle CDK inhibitor p27 and p21 were upregulated with simultaneous downregulation of cyclin D1 and cyclin E in HCT-116 cells. This was accompanied by apoptosis, as depicted by decrease in Bcl-2/Bax ratio, with increase in active caspases-3 and caspase-9, cleavage of PARP-1, the generation of reactive oxygen species (ROS), and the loss of mitochondrial membrane potential. The findings established that 3BH induced cell death in HCT-116 cells by modulating PI3K/Akt signaling axis, impeding cell cycle, and instigating apoptosis.
Subject(s)
Antineoplastic Agents, Phytogenic/pharmacology , Apoptosis/drug effects , Lamiaceae/chemistry , Signal Transduction/drug effects , Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/isolation & purification , Cell Line, Tumor , Cyclin D1/genetics , Cyclin D1/metabolism , Cyclin-Dependent Kinase Inhibitor p21/genetics , Cyclin-Dependent Kinase Inhibitor p21/metabolism , Down-Regulation/drug effects , Drug Screening Assays, Antitumor , G1 Phase Cell Cycle Checkpoints/drug effects , HCT116 Cells , Humans , Lamiaceae/metabolism , Membrane Potential, Mitochondrial/drug effects , Phosphatidylinositol 3-Kinases/genetics , Phosphatidylinositol 3-Kinases/metabolism , Plant Extracts/chemistry , Proto-Oncogene Proteins c-akt/metabolism , Proto-Oncogene Proteins c-bcl-2/metabolism , Reactive Oxygen Species/metabolism , Sitosterols/chemistry , Sitosterols/pharmacology , bcl-2-Associated X Protein/metabolismABSTRACT
Highly stable gold and silver nanoparticles were synthesized by use of an arabinoglucan from Lallemantia royleana seeds without additional use of reducing or stabilizing agents. The mechanism involved the reduction potential of the hemicellulose as verified by cyclic voltammetry. The arabinoglucan used was substantially free from ferulic acid and phenolic content, suggesting the inherent reducing potential of arabinoglucan for gold and silver ions. The synthesized nanoparticles exhibited surface plasmon resonance maxima at 515 nm (gold) and 397 nm (silver) corresponding to sizes of 10 nm and 8 nm, respectively. The zeta potential values were -24.1 mV (gold) and -22.3 mV (silver). The silver nanoparticles showed potential for application in surface-enhanced Raman spectroscopy. Gold nanoparticles were found to be non-toxic, whereas silver nanoparticles exhibited dose-dependent biological activities and found to be cytotoxic against brine shrimps and HeLa cell lines and the tumours caused by A. tumefaciens.
Subject(s)
Gold/chemistry , Green Chemistry Technology/methods , Lamiaceae/metabolism , Metal Nanoparticles/chemistry , Plant Extracts/chemistry , Silver/chemistry , Animals , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacology , Artemia , Glucans/chemistry , HeLa Cells , Humans , Microbial Sensitivity Tests , Particle Size , Polysaccharides/chemistry , Salts , Seeds/chemistry , Surface Plasmon ResonanceABSTRACT
BACKGROUND: Our current study aimed to evaluate the effect of an Glechoma hederacea extract (Hitrechol®) in normal rats and gallstone diseased mice to explore its underlying mechanisms. Normal rats and C57BL/6 mice with/without cholesterol gallstone were used in this study. METHODS: To monitor the effect of Hitrechol® on bile secretion, bile flow rates at 15 min interval until 2 h post-dosing in normal rats treated with vehicle and Hitrechol® were compared using multiple t-test with a p < 0.05 considered as statistically significant different. To further evaluate the effect of Hitrechol® against the development of gallstone in lithogenic diet treated mice, mice were treated with vehicle or Hitrechol® (QD-once daily or TID-three times daily) for 3 weeks followed by comparing the levels of bile composition among the treatment groups. In addition, the anti-oxidative biomarkers in liver and anti-inflammatory biomarkers in serum were detected and compared among all the treatment groups to evaluate the hepato-protective effect of Hitrechol®. The obtained levels of biomarkers and bile composition were compared among different treatment groups using one-way ANOVA tests followed by Tukey's multiple comparisons with p < 0.05 considered as statistically significant. RESULTS: Despite no significant impact on the bile flow rate, Hitrechol® TID treatment dramatically decreased size and amount of gallstone crystals and total cholesterol level (p < 0.05), as well as total bile acid (p < 0.05) and several types of bile acid (p < 0.05) levels in gallstone disease model mice. Hitrechol® TID treatment could significantly decrease the frequencies of hepatocyte necrosis and lipid aggregation notably as well as increase the antioxidant enzyme level (p < 0.05) in the liver. CONCLUSIONS: Our findings for the first time demonstrated the beneficial effect of Hitrechol® against gallstone via its litholytic, liver-protective and antioxidant activities.
Subject(s)
Antioxidants/pharmacology , Gallstones/prevention & control , Lamiaceae/metabolism , Plant Extracts/pharmacology , Animals , Disease Models, Animal , Mice , Mice, Inbred C57BL , Rats , RodentiaABSTRACT
The metabolites produced by plants can be enhanced by plant tissue culture. In Premma puberula Pamp., the pectin content in leaves is 30 %-40 %, and it is widely used in the food industry and medicine. However, inefficient propagation has seriously restricted the utilization of pectin resources. Therefore, we established an efficient micropropagation technology for P. puberula through comparative analysis in mature leaves of regenerated and conventionally propagated plants. The results showed that the pectin composition of their leaves was similar in terms of galacturonic acid, monosaccharide composition, degree of esterification, functional groups, nuclear magnetic resonance spectrum and morphological characteristics. Furthermore, micropropagated plants had better hardness, gumminess and chewiness characteristics than conventionally propagated plants and were similar in emulsion stability, adhesiveness, springiness, cohesiveness and viscoelasticity. Therefore, micropropagation technology will provide an important guarantee for the industrial production of pectin from P. puberula. The technical essentials include callus induction, embryoid formation, and root induction, followed by acclimatization and transplanting.
Subject(s)
Lamiaceae/metabolism , Pectins/metabolism , Emulsifying Agents/chemistry , Esterification , Hexuronic Acids/analysis , Lamiaceae/cytology , Monosaccharides/analysis , Pectins/chemistry , Pectins/isolation & purification , Plant Leaves/cytology , Plant Leaves/metabolism , ViscosityABSTRACT
Obesity is becoming a worldwide epidemic, especially in industrialized countries. We hereby report a methanolic extract of Mesona procumbens (known as Hsian-tsao in Taiwan) significantly inhibits lipid accumulation in 3T3-L1 adipocytes, and eight new primeverose derivatives, mesonosides A-H (1-8), were isolated from the methanolic extract of M. procumbens. Structural elucidation of 1-8 was established by spectroscopic methods, especially 2D NMR techniques (1H-1H COSY, HSQC, HMBC, and NOESY) and HRESIMS. Anti-obesity evaluation revealed that isolates 1-5, 7, and 8 showed inhibitory effects on lipid accumulation and protein levels of adipogenic transcription factor, PPARγ and C/EBPα in 3T3-L1 cells. Our study suggests that M. procumbens extract including new primeverose isolates may be potentially used as a natural source to ameliorate fat accumulation and even obesity.
Subject(s)
Adipogenesis , Lamiaceae , 3T3-L1 Cells , Animals , CCAAT-Enhancer-Binding Protein-alpha/genetics , CCAAT-Enhancer-Binding Protein-alpha/metabolism , CCAAT-Enhancer-Binding Protein-alpha/pharmacology , Lamiaceae/metabolism , Lipids , Mice , Obesity/metabolism , PPAR gamma/genetics , PPAR gamma/metabolism , PPAR gamma/pharmacology , Plant Extracts/pharmacologyABSTRACT
The extract of Elsholtzia ciliata aerial parts was subjected to bio-guided isolation using the intercellular ROS reduction in J774A.1 macrophages to monitor the anti-oxidative activity. Fifteen compounds were isolated from the active fractions including eleven flavonoids (vitexin, pedalin, luteolin-7-O-ß-d-glucopyranoside, apigenin-5-O-ß-d-glucopyranoside, apigenin-7-O-ß-d-glucopyranoside, chrysoeriol-7-O-ß-d-glucopyranoside, 7,3'-dimethoxyluteolin-6-O-ß-d-glucopyranoside, luteolin, 5,6,4'-trihydroxy-7,3'-dimethoxyflavone, 5-hydroxy-6,7-dimethoxyflavone (compound 13), 5-hydroxy-7,8-dimethoxyflavone); three hydroxycinnamic acid derivatives (caffeic acid, 4-(E)-caffeoyl-l-threonic acid, 4-O-(E)-p-coumaroyl-l-threonic acid) and one fatty acid (α-linolenic acid). The biological evaluation of these compounds (10-2.5â µm) indicated that all of them exerted good antioxidant and anti-inflammatory activities, in particular compound 13.
Subject(s)
Lamiaceae/chemistry , Nitric Oxide/metabolism , Plant Extracts/chemistry , Reactive Oxygen Species/metabolism , Animals , Antioxidants/chemistry , Cell Line , Cell Proliferation/drug effects , Flavonoids/chemistry , Flavonoids/isolation & purification , Flavonoids/pharmacology , Hydrogen Peroxide/pharmacology , Lamiaceae/metabolism , Macrophages/cytology , Macrophages/drug effects , Macrophages/metabolism , Mice , Oxidative Stress/drug effects , VietnamABSTRACT
BACKGROUND: To investigate the effects of salinity as a serious environmental limiter of productivity on the yield-related traits of Lallemantia iberica, a split-plot experiment was performed for 2 years (2017-2018) based on a randomized complete block design with three replications at Urmia University (37°33'09â³N, 45°05'53â³E). The main plots included salinity stress at two levels (6.72 dS m-1 , and 0.91 dS m-1 as control), and subplots were inoculants at four levels (Funneliformis mosseae, Thiobacillus sp., F. mosseae + Thiobacillus sp., and no inoculation). RESULTS: In the saline condition, serious reductions in yield and yield components (numbers of capsules per plant, seeds per capsule, and seeds per plant, 1000-seed weight, seed and biological yields), concentrations of leaf phosphorus and potassium, and relative mycorrhizal dependency were observed, but against the harvest index the leaf sulfur and sodium contents were increased. Moreover, all morphological traits (plant height, number of branches and leaves, leaf weight, stem weight, and ratio of leaf weight to stem weight) were decreased under salinity conditions. Mycorrhizal inoculation enhanced the salinity-induced reduction of yield and morphological traits to some extent. Inoculation with Thiobacillus had superiority in some of the yield and morphological characteristics compared with those in the non-inoculated plants. CONCLUSION: Salinity stress can significantly affect the yield, morphological characteristics, nutrients content, and mycorrhizal dependency of L. iberica plants. This study exhibited the significant effects of single and simultaneous applications of F. mosseae and Thiobacillus on plant growth and yield in saline soils. © 2020 Society of Chemical Industry.
Subject(s)
Agricultural Inoculants/metabolism , Fungi/metabolism , Lamiaceae/growth & development , Lamiaceae/metabolism , Mycorrhizae/metabolism , Sulfur/metabolism , Thiobacillus/metabolism , Lamiaceae/microbiology , Oxidation-Reduction , Phosphorus/metabolism , Plant Leaves/growth & development , Plant Leaves/metabolism , Salinity , Sodium Chloride/metabolismABSTRACT
BACKGROUND: A wide variety of secondary metabolites are synthesized from primary metabolites by plants which have a vast range in pharmaceutical, food additive and industrial applications. In recent years, the use of elicitors has opened a novel approach for the production of secondary metabolite compounds. Dracocephalum kotschyi is a valuable herb due to pharmaceutical compounds like rosmarinic acid, quercetin and apigenin. In the current study, foliar application of chitosan (0, 100, 400 mg L-1 ) as an elicitor was used. RESULTS: After chitosan treatment, the amounts of hydrogen peroxide (H2 O2 ) increased and the plant was able to increase the activities of enzymatic (guaiacol peroxidase, catalase and phenylalanine ammonium lyase) and non-enzymatic (total phenols and flavonoids) defensive metabolites. Also, foliar spray of chitosan promoted nutrient absorption which led to the accumulation of macroelements in the plant. CONCLUSIONS: Chitosan was found to be a very effective elicitor for improving rosmarinic acid and quercetin content (up to 13-fold). Also, the content of apigenin (anticancer flavonoid) showed 16-fold enhancement compared to the control. Therefore, the treatment of D. kotschyi leaves with chitosan caused a very large increase in the induction and production of important pharmaceutical compounds such as rosmarinic acid and quercetin. © 2020 Society of Chemical Industry.
Subject(s)
Chitosan/pharmacology , Lamiaceae/drug effects , Lamiaceae/metabolism , Secondary Metabolism/drug effects , Biological Transport/drug effects , Catalase/metabolism , Cinnamates/analysis , Cinnamates/metabolism , Depsides/analysis , Depsides/metabolism , Hydrogen Peroxide/metabolism , Nutrients/metabolism , Peroxidase/metabolism , Plant Leaves/drug effects , Plant Leaves/metabolism , Plant Proteins/metabolism , Plants, Medicinal/drug effects , Plants, Medicinal/metabolism , Quercetin/analysis , Quercetin/metabolism , Rosmarinic AcidABSTRACT
Here we report the comprehensive characterization of the secondary metabolites from the leaves of Colebrookea oppositifolia Smith, a species used as medicinal plant in the traditional medicine of Nepal. Phytochemical screening of bioactives was performed using an integrated LC-MSn and high resolution MS (Mass Spectrometry) approach. Forty-three compounds were tentatively identified, mainly aglyconic and glycosilated flavonoids and phenolic acids, as well as other bioactives such as coumarins and terpenes were detected. Furthermore, the NF-κB and AP-1 inhibitory activity of C. oppositifolia extract were evaluated, as well as its cytotoxicity against THP-1 cells, in order to assess the potential use of this herb as a source of anti-inflammatory and cytotoxic compounds. The results so far obtained indicate that C. oppositifolia leaves extract could significantly reduce the viability of THP-1 cells (IC50 = 6.2 ± 1.2 µg/mL), as well as the activation of both NF-κB and AP-1 at the concentration of 2 µg/mL. Our results indicate that Nepalese C. oppositifolia is a valuable source of anti-inflammatory and cytotoxic compounds. The phytochemical composition reported here can partially justify the traditional uses of C. oppositifolia in Nepal, especially in the treatment of inflammatory diseases, although further research will be needed to assess the full potential of this species.
Subject(s)
Anti-Inflammatory Agents/pharmacology , Lamiaceae/metabolism , NF-kappa B/antagonists & inhibitors , Plant Extracts/pharmacology , Plants, Medicinal/metabolism , Transcription Factor AP-1/antagonists & inhibitors , Anti-Inflammatory Agents/isolation & purification , Anti-Inflammatory Agents/toxicity , Chromatography, Liquid , Flavonoids/analysis , Flavonoids/isolation & purification , Humans , Hydroxybenzoates/analysis , Hydroxybenzoates/isolation & purification , Mass Spectrometry , Metabolome , Methanol , Nepal , Plant Extracts/chemistry , Plant Extracts/isolation & purification , Plant Extracts/toxicity , Plant Leaves/metabolism , THP-1 CellsABSTRACT
The objective of this study was to quantify the phenolic compounds and to evaluate and compare the biological activities of the ethyl acetate (EtOAc), methanolic (MeOH) and aqueous extracts from the Micromeria nervosa aerial parts, based on their antioxidant activity and enzymatic inhibition. Total phenolic and flavonoid contents were calculated and individual compo3unds were detected using LC-ESI-MS/MS. The antioxidant activity was determined using six different assays while enzymatic activity was determined by α-amylase and tyrosinase enzyme inhibition. The main phenolic constituents detected in the extracts were rosmarinic acid. In the antioxidant assays the aqueous extract was shown to be more efficient than the others. The EtOAc and MeOH extracts presented higher inhibitory activity with respect to α-amylase and tyrosinase. Regardless of the solvent, the results suggest M. nervosa aerial extracts present a biological potential due to their antioxidant activity and enzymatic inhibition.