ABSTRACT
The synaptic organization of thalamic inputs to motor cortices remains poorly understood in primates. Thus, we compared the regional and synaptic connections of vGluT2-positive thalamocortical glutamatergic terminals in the supplementary motor area (SMA) and the primary motor cortex (M1) between control and MPTP-treated parkinsonian monkeys. In controls, vGluT2-containing fibers and terminal-like profiles invaded layer II-III and Vb of M1 and SMA. A significant reduction of vGluT2 labeling was found in layer Vb, but not in layer II-III, of parkinsonian animals, suggesting a potential thalamic denervation of deep cortical layers in parkinsonism. There was a significant difference in the pattern of synaptic connectivity in layers II-III, but not in layer Vb, between M1 and SMA of control monkeys. However, this difference was abolished in parkinsonian animals. No major difference was found in the proportion of perforated versus macular post-synaptic densities at thalamocortical synapses between control and parkinsonian monkeys in both cortical regions, except for a slight increase in the prevalence of perforated axo-dendritic synapses in the SMA of parkinsonian monkeys. Our findings suggest that disruption of the thalamic innervation of M1 and SMA may underlie pathophysiological changes of the motor thalamocortical loop in the state of parkinsonism.
Subject(s)
Motor Cortex/ultrastructure , Parkinsonian Disorders/pathology , Post-Synaptic Density/ultrastructure , Thalamus/ultrastructure , 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine , Animals , Female , Macaca mulatta , Male , Neural Pathways/ultrastructure , Neurotoxins , Vesicular Glutamate Transport Protein 2/metabolismABSTRACT
Learning a novel motor skill is dependent both on regional changes within the primary motor cortex (M1) contralateral to the active hand and also on modulation between and within anatomically distant but functionally connected brain regions. Interregional changes are particularly important in functional recovery after stroke, when critical plastic changes underpinning behavioral improvements are observed in both ipsilesional and contralesional M1s. It is increasingly understood that reduction in GABA in the contralateral M1 is necessary to allow learning of a motor task. However, the physiological mechanisms underpinning plasticity within other brain regions, most importantly the ipsilateral M1, are not well understood. Here, we used concurrent two-voxel magnetic resonance spectroscopy to simultaneously quantify changes in neurochemicals within left and right M1s in healthy humans of both sexes in response to transcranial direct current stimulation (tDCS) applied to left M1. We demonstrated a decrease in GABA in both the stimulated (left) and nonstimulated (right) M1 after anodal tDCS, whereas a decrease in GABA was only observed in nonstimulated M1 after cathodal stimulation. This GABA decrease in the nonstimulated M1 during cathodal tDCS was negatively correlated with microstructure of M1:M1 callosal fibers, as quantified by diffusion MRI, suggesting that structural features of these fibers may mediate GABA decrease in the unstimulated region. We found no significant changes in glutamate. Together, these findings shed light on the interactions between the two major network nodes underpinning motor plasticity, offering a potential framework from which to optimize future interventions to improve motor function after stroke.SIGNIFICANCE STATEMENT Learning of new motor skills depends on modulation both within and between brain regions. Here, we use a novel two-voxel magnetic resonance spectroscopy approach to quantify GABA and glutamate changes concurrently within the left and right primary motor cortex (M1) during three commonly used transcranial direct current stimulation montages: anodal, cathodal, and bilateral. We also examined how the neurochemical changes in the unstimulated hemisphere were related to white matter microstructure between the two M1s. Our results provide insights into the neurochemical changes underlying motor plasticity and may therefore assist in the development of further adjunct therapies.
Subject(s)
Motor Cortex/metabolism , Motor Skills/physiology , Transcranial Direct Current Stimulation , gamma-Aminobutyric Acid/metabolism , Adult , Corpus Callosum/ultrastructure , Diffusion Magnetic Resonance Imaging , Dominance, Cerebral , Female , Glutamic Acid/metabolism , Humans , Magnetic Resonance Spectroscopy/methods , Male , Motor Cortex/chemistry , Motor Cortex/ultrastructure , Nerve Fibers, Myelinated/ultrastructure , Neuronal Plasticity , Young AdultABSTRACT
The hypothalamic neurohormone oxytocin decreases food intake via largely unexplored mechanisms. We investigated the central nervous mediation of oxytocin's hypophagic effect in comparison to its impact on the processing of generalized rewards. Fifteen fasted normal-weight, young men received intranasal oxytocin (24 IU) or placebo before functional magnetic resonance imaging (fMRI) measurements of brain activity during exposure to food stimuli and a monetary incentive delay task (MID). Subsequently, ad-libitum breakfast intake was assessed. Oxytocin compared to placebo increased activity in the ventromedial prefrontal cortex, supplementary motor area, anterior cingulate, and ventrolateral prefrontal cortices in response to high- vs. low-calorie food images in the fasted state, and reduced calorie intake by 12%. During anticipation of monetary rewards, oxytocin compared to placebo augmented striatal, orbitofrontal and insular activity without altering MID performance. We conclude that during the anticipation of generalized rewards, oxytocin stimulates dopaminergic reward-processing circuits. In contrast, oxytocin restrains food intake by enhancing the activity of brain regions that exert cognitive control, while concomitantly increasing the activity of structures that process food reward value. This pattern points towards a specific role of oxytocin in the regulation of eating behaviour in humans that might be of relevance for potential clinical applications.
Subject(s)
Eating/drug effects , Gyrus Cinguli/physiology , Motor Cortex/physiology , Oxytocin/physiology , Prefrontal Cortex/physiology , Administration, Intranasal , Adult , Brain Mapping/methods , Cognition/drug effects , Fasting , Gyrus Cinguli/ultrastructure , Healthy Volunteers , Humans , Magnetic Resonance Imaging/methods , Male , Motivation/drug effects , Motor Cortex/ultrastructure , Oxytocin/administration & dosage , Prefrontal Cortex/ultrastructure , RewardABSTRACT
The traditional classification of primary motor cortex (M1) as an agranular area has been challenged recently when a functional layer 4 (L4) was reported in M1. L4 is the principal target for thalamic input in sensory areas, which raises the question of how thalamocortical synapses formed in M1 in the mouse compare with those in neighboring sensory cortex (S1). We identified thalamic boutons by their immunoreactivity for the vesicular glutamate transporter 2 (VGluT2) and performed unbiased disector counts from electron micrographs. We discovered that the thalamus contributed proportionately only half as many synapses to the local circuitry of L4 in M1 compared with S1. Furthermore, thalamic boutons in M1 targeted spiny dendrites exclusively, whereas â¼9% of synapses were formed with dendrites of smooth neurons in S1. VGluT2+ boutons in M1 were smaller and formed fewer synapses per bouton on average (1.3 vs 2.1) than those in S1, but VGluT2+ synapses in M1 were larger than in S1 (median postsynaptic density areas of 0.064 µm2 vs 0.042 µm2). In M1 and S1, thalamic synapses formed only a small fraction (12.1% and 17.2%, respectively) of all of the asymmetric synapses in L4. The functional role of the thalamic input to L4 in M1 has largely been neglected, but our data suggest that, as in S1, the thalamic input is amplified by the recurrent excitatory connections of the L4 circuits. The lack of direct thalamic input to inhibitory neurons in M1 may indicate temporal differences in the inhibitory gating in L4 of M1 versus S1.SIGNIFICANCE STATEMENT Classical interpretations of the function of primary motor cortex (M1) emphasize its lack of the granular layer 4 (L4) typical of sensory cortices. However, we show here that, like sensory cortex (S1), mouse M1 also has the canonical circuit motif of a core thalamic input to the middle cortical layer and that thalamocortical synapses form a small fraction (M1: 12%; S1: 17%) of all asymmetric synapses in L4 of both areas. Amplification of thalamic input by recurrent local circuits is thus likely to be a significant mechanism in both areas. Unlike M1, where thalamocortical boutons typically form a single synapse, thalamocortical boutons in S1 usually formed multiple synapses, which means they can be identified with high probability in the electron microscope without specific labeling.
Subject(s)
Motor Cortex/ultrastructure , Somatosensory Cortex/ultrastructure , Thalamus/ultrastructure , Animals , Imaging, Three-Dimensional , Male , Mice , Mice, Inbred C57BL , Microscopy, Electron , Models, Anatomic , Motor Cortex/metabolism , Neural Pathways/physiology , Neural Pathways/ultrastructure , Phosphopyruvate Hydratase/metabolism , Presynaptic Terminals/metabolism , Presynaptic Terminals/ultrastructure , Somatosensory Cortex/metabolism , Synapses/metabolism , Synapses/ultrastructure , Synaptic Vesicles/metabolism , Synaptic Vesicles/ultrastructure , Thalamus/metabolism , Vesicular Glutamate Transport Protein 2/metabolism , Vesicular Glutamate Transport Protein 2/ultrastructureABSTRACT
Deafening elicits a deterioration of learned vocalization, in both humans and songbirds. In songbirds, learned vocal plasticity has been shown to depend on the basal ganglia-cortical circuit, but the underlying cellular basis remains to be clarified. Using confocal imaging and electron microscopy, we examined the effect of deafening on dendritic spines in avian vocal motor cortex, the robust nucleus of the arcopallium (RA), and investigated the role of the basal ganglia circuit in motor cortex plasticity. We found rapid structural changes to RA dendritic spines in response to hearing loss, accompanied by learned song degradation. In particular, the morphological characters of RA spine synaptic contacts between 2 major pathways were altered differently. However, experimental disruption of the basal ganglia circuit, through lesions in song-specialized basal ganglia nucleus Area X, largely prevented both the observed changes to RA dendritic spines and the song deterioration after hearing loss. Our results provide cellular evidence to highlight a key role of the basal ganglia circuit in the motor cortical plasticity that underlies learned vocal plasticity.
Subject(s)
Auditory Pathways/physiopathology , Basal Ganglia/physiology , Deafness/pathology , Dendritic Spines/physiology , Motor Cortex/pathology , Vocalization, Animal , Analysis of Variance , Animals , Biotin/analogs & derivatives , Deafness/etiology , Dendritic Spines/ultrastructure , Dextrans , Disease Models, Animal , Electrolysis/adverse effects , Finches , High Vocal Center/physiopathology , Male , Microscopy, Confocal , Microscopy, Electron, Transmission , Motor Cortex/ultrastructure , Synapses/pathology , Synapses/ultrastructureABSTRACT
UNLABELLED: Arginase 1 deficiency is a urea cycle disorder associated with hyperargininemia, spastic diplegia, loss of ambulation, intellectual disability, and seizures. To gain insight on how loss of arginase expression affects the excitability and synaptic connectivity of the cortical neurons in the developing brain, we used anatomical, ultrastructural, and electrophysiological techniques to determine how single-copy and double-copy arginase deletion affects cortical circuits in mice. We find that the loss of arginase 1 expression results in decreased dendritic complexity, decreased excitatory and inhibitory synapse numbers, decreased intrinsic excitability, and altered synaptic transmission in layer 5 motor cortical neurons. Hepatic arginase 1 gene therapy using adeno-associated virus rescued nearly all these abnormalities when administered to neonatal homozygous knock-out animals. Therefore, gene therapeutic strategies can reverse physiological and anatomical markers of arginase 1 deficiency and therefore may be of therapeutic benefit for the neurological disabilities in this syndrome. SIGNIFICANCE STATEMENT: These studies are one of the few investigations to try to understand the underlying neurological dysfunction that occurs in urea cycle disorders and the only to examine arginase deficiency. We have demonstrated by multiple modalities that, in murine layer 5 cortical neurons, a gradation of abnormalities exists based on the functional copy number of arginase: intrinsic excitability is altered, there is decreased density in asymmetrical and perisomatic synapses, and analysis of the dendritic complexity is lowest in the homozygous knock-out. With neonatal administration of adeno-associated virus expressing arginase, there is near-total recovery of the abnormalities in neurons and cortical circuits, supporting the concept that neonatal gene therapy may prevent the functional abnormalities that occur in arginase deficiency.
Subject(s)
Arginase/therapeutic use , Genetic Therapy , Hyperargininemia/pathology , Hyperargininemia/therapy , Motor Cortex/physiology , Recovery of Function/physiology , Action Potentials/drug effects , Action Potentials/physiology , Ammonia/blood , Animals , Animals, Newborn , Arginase/genetics , Arginase/metabolism , Disease Models, Animal , Hyperargininemia/blood , In Vitro Techniques , Mice , Mice, Transgenic , Motor Cortex/cytology , Motor Cortex/ultrastructure , Nerve Net/pathology , Nerve Net/physiology , Nerve Net/ultrastructure , Neurons/physiology , Neurons/ultrastructure , Picrotoxin/pharmacology , Sodium Channel Blockers/pharmacology , Synapses/ultrastructure , Tetrodotoxin/pharmacologyABSTRACT
The motor cortex (M1) is classically considered an agranular area, lacking a distinct layer 4 (L4). Here, we tested the idea that M1, despite lacking a cytoarchitecturally visible L4, nevertheless possesses its equivalent in the form of excitatory neurons with input-output circuits like those of the L4 neurons in sensory areas. Consistent with this idea, we found that neurons located in a thin laminar zone at the L3/5A border in the forelimb area of mouse M1 have multiple L4-like synaptic connections: excitatory input from thalamus, largely unidirectional excitatory outputs to L2/3 pyramidal neurons, and relatively weak long-range corticocortical inputs and outputs. M1-L4 neurons were electrophysiologically diverse but morphologically uniform, with pyramidal-type dendritic arbors and locally ramifying axons, including branches extending into L2/3. Our findings therefore identify pyramidal neurons in M1 with the expected prototypical circuit properties of excitatory L4 neurons, and question the traditional assumption that motor cortex lacks this layer.
Subject(s)
Action Potentials/physiology , Motor Cortex/physiology , Pyramidal Cells/physiology , Synapses/physiology , Synaptic Potentials/physiology , Adenoviridae/genetics , Animals , Axons/physiology , Axons/ultrastructure , Dendrites/physiology , Dendrites/ultrastructure , Fluorescent Dyes , Genetic Vectors , Mice , Microspheres , Microtomy , Motor Cortex/ultrastructure , Pyramidal Cells/ultrastructure , Stereotaxic Techniques , Synapses/ultrastructure , Synaptic Transmission , Thalamus/physiology , Thalamus/ultrastructure , Tissue Culture TechniquesABSTRACT
Intraperitoneal injection of the Gram-negative bacterial endotoxin lipopolysaccharide (LPS) elicits a rapid innate immune response. While this systemic inflammatory response can be destructive, tolerable low doses of LPS render the brain transiently resistant to subsequent injuries. However, the mechanism by which microglia respond to LPS stimulation and participate in subsequent neuroprotection has not been documented. In this study, we first established a novel LPS treatment paradigm where mice were injected intraperitoneally with 1.0 mg/kg LPS for four consecutive days to globally activate CNS microglia. By using a reciprocal bone marrow transplantation procedure between wild-type and Toll-like receptor 4 (TLR4) mutant mice, we demonstrated that the presence of LPS receptor (TLR4) is not required on hematogenous immune cells but is required on cells that are not replaced by bone marrow transplantation, such as vascular endothelia and microglia, to transduce microglial activation and neuroprotection. Furthermore, we showed that activated microglia physically ensheathe cortical projection neurons, which have reduced axosomatic inhibitory synapses from the neuronal perikarya. In line with previous reports that inhibitory synapse reduction protects neurons from degeneration and injury, we show here that neuronal cell death and lesion volumes are significantly reduced in LPS-treated animals following experimental brain injury. Together, our results suggest that activated microglia participate in neuroprotection and that this neuroprotection is likely achieved through reduction of inhibitory axosomatic synapses. The therapeutic significance of these findings rests not only in identifying neuroprotective functions of microglia, but also in establishing the CNS location of TLR4 activation.
Subject(s)
Brain Injuries/drug therapy , Lipopolysaccharides/administration & dosage , Microglia/drug effects , Neuroprotective Agents/administration & dosage , Toll-Like Receptor 4/metabolism , Animals , Antigens, CD/metabolism , Apoptosis/drug effects , Bone Marrow Transplantation , Brain Injuries/pathology , Brain Injuries/surgery , Bromodeoxyuridine/metabolism , Cell Proliferation/drug effects , Central Nervous System/cytology , Central Nervous System/drug effects , Disease Models, Animal , Dose-Response Relationship, Drug , Drug Administration Schedule , Flow Cytometry , Immunity, Innate/drug effects , In Situ Nick-End Labeling , Mice , Mice, Inbred C3H , Mice, Inbred C57BL , Mice, Knockout , Microarray Analysis , Microglia/metabolism , Microglia/ultrastructure , Microscopy, Immunoelectron , Motor Cortex/pathology , Motor Cortex/ultrastructure , Synapses/genetics , Synapses/metabolism , Synapses/ultrastructure , Toll-Like Receptor 4/deficiency , Transplantation ChimeraABSTRACT
The pathophysiology of mood and psychotic disorders, including unipolar depression (UPD), bipolar disorder (BPD) and schizophrenia (SCHZ), is largely unknown. Numerous studies, from molecular to neuroimaging, indicate that some individuals with these disorders have impaired brain energy metabolism evidenced by abnormal glucose metabolism and mitochondrial dysfunction. However, underlying mechanisms are unclear. A critical feature of brain energy metabolism is attachment to the outer mitochondrial membrane (OMM) of hexokinase 1 (HK1), an initial and rate-limiting enzyme of glycolysis. HK1 attachment to the OMM greatly enhances HK1 enzyme activity and couples cytosolic glycolysis to mitochondrial oxidative phosphorylation, through which the cell produces most of its adenosine triphosphate (ATP). HK1 mitochondrial attachment is also important to the survival of neurons and other cells through prevention of apoptosis and oxidative damage. Here we show, for the first time, a decrease in HK1 attachment to the OMM in postmortem parietal cortex brain tissue of individuals with UPD, BPD and SCHZ compared to tissue from controls without psychiatric illness. Furthermore, we show that HK1 mitochondrial detachment is associated with increased activity of the polyol pathway, an alternative, anaerobic pathway of glucose metabolism. These findings were observed in samples from both medicated and medication-free individuals. We propose that HK1 mitochondrial detachment could be linked to these disorders through impaired energy metabolism, increased vulnerability to oxidative stress, and impaired brain growth and development.
Subject(s)
Bipolar Disorder/pathology , Brain/ultrastructure , Energy Metabolism/physiology , Hexokinase/metabolism , Mitochondria/enzymology , Schizophrenia/pathology , Adenosine Triphosphate/metabolism , Adult , Aged , Analysis of Variance , Brain/pathology , Female , Humans , Male , Middle Aged , Motor Cortex/pathology , Motor Cortex/ultrastructure , Parietal Lobe/pathology , Parietal Lobe/ultrastructure , Postmortem ChangesABSTRACT
NrCAM is a neural cell adhesion molecule of the L1 family that has been linked to autism spectrum disorders, a disease spectrum in which abnormal thalamocortical connectivity may contribute to visual processing defects. Here we show that NrCAM interaction with neuropilin-2 (Npn-2) is critical for semaphorin 3F (Sema3F)-induced guidance of thalamocortical axon subpopulations at the ventral telencephalon (VTe), an intermediate target for thalamic axon sorting. Genetic deletion of NrCAM or Npn-2 caused contingents of embryonic thalamic axons to misproject caudally in the VTe. The resultant thalamocortical map of NrCAM-null mutants showed striking mistargeting of motor and somatosensory thalamic axon contingents to the primary visual cortex, but retinogeniculate targeting and segregation were normal. NrCAM formed a molecular complex with Npn-2 in brain and neural cells, and was required for Sema3F-induced growth cone collapse in thalamic neuron cultures, consistent with a vital function for NrCAM in Sema3F-induced axon repulsion. NrCAM-null mice displayed reduced responses to visual evoked potentials recorded from layer IV in the binocular zone of primary visual cortex (V1), particularly when evoked from the ipsilateral eye, indicating abnormal visual acuity and ocularity. These results demonstrate that NrCAM is required for normal maturation of cortical visual acuity, and suggest that the aberrant projection of thalamic motor and somatosensory axons to the visual cortex in NrCAM-null mutant mice impairs cortical functions.
Subject(s)
Axons/physiology , Cell Adhesion Molecules/physiology , Motor Cortex/ultrastructure , Somatosensory Cortex/ultrastructure , Thalamus/ultrastructure , Visual Acuity , Visual Cortex/ultrastructure , Animals , Cell Adhesion Molecules/genetics , Evoked Potentials, Visual , Female , Growth Cones/physiology , Male , Membrane Proteins/physiology , Mice , Mice, Knockout , Motor Cortex/embryology , Motor Cortex/growth & development , Nerve Tissue Proteins/physiology , Neuropilin-2/genetics , Neuropilin-2/physiology , Somatosensory Cortex/embryology , Somatosensory Cortex/growth & development , Thalamus/embryology , Thalamus/growth & development , Visual Cortex/embryology , Visual Cortex/growth & developmentABSTRACT
The details and functional significance of the intrinsic horizontal connections between neurons in the motor cortex (MCx) remain to be clarified. To further elucidate the nature of this intracortical connectivity pattern, experiments were done on the MCx of three cats. The anterograde tracer biocytin was ejected iontophoretically in layers II, III, and V. Some 30-50 neurons within a radius of approximately 250 microm were thus stained. The functional output of the motor cortical point at which biocytin was injected, and of the surrounding points, was identified by microstimulation and electromyographic recordings. The axonal arborizations of the stained neurons were traced under camera lucida. The axon collaterals were extensive, reaching distances of Subject(s)
Motor Cortex/physiology
, Neurons/physiology
, Animals
, Axons/physiology
, Axons/ultrastructure
, Cats
, Cluster Analysis
, Dendritic Spines/physiology
, Dendritic Spines/ultrastructure
, Electric Stimulation
, Electromyography
, Lysine/analogs & derivatives
, Male
, Microelectrodes
, Microscopy, Electron
, Motor Cortex/cytology
, Motor Cortex/ultrastructure
, Multivariate Analysis
, Muscle, Skeletal/innervation
, Muscle, Skeletal/physiology
, Neural Pathways/cytology
, Neural Pathways/physiology
, Neural Pathways/ultrastructure
, Neuronal Tract-Tracers
, Neurons/cytology
, Neurons/ultrastructure
, Presynaptic Terminals/physiology
, Presynaptic Terminals/ultrastructure
, Pyramidal Cells/cytology
, Pyramidal Cells/physiology
, Pyramidal Cells/ultrastructure
, Synapses/physiology
, Synapses/ultrastructure
ABSTRACT
Experience-dependent regulation of synaptic strength in the horizontal connections in layer 1 of the primary motor cortex is likely to play an important role in motor learning. Dendritic spines, the primary sites of excitatory synapses in the brain, are known to change shape in response to various experimental stimuli. We used a rat motor learning model to examine connection strength via field recordings in slices and confocal imaging of labeled spines to explore changes induced solely by learning a simple motor task. We report that motor learning increases response size, while transiently occluding long-term potentiation (LTP) and increasing spine width in layer 1. This demonstrates learning-induced changes in behavior, synaptic responses, and structure in the same animal, suggesting that an LTP-like process in the motor cortex mediates the initial learning of a skilled task.
Subject(s)
Dendritic Spines/physiology , Dendritic Spines/ultrastructure , Learning/physiology , Motor Cortex/physiology , Motor Cortex/ultrastructure , Neuronal Plasticity/physiology , Amino Acids , Analysis of Variance , Animals , Behavior, Animal , Dose-Response Relationship, Radiation , Electric Stimulation/methods , Estrous Cycle/physiology , Female , In Vitro Techniques , Long-Term Potentiation/physiology , Long-Term Potentiation/radiation effects , Microscopy, Confocal/methods , Motor Skills/physiology , Rats , Rats, Sprague-DawleyABSTRACT
Cortical stimulation (CS) as a means to modulate regional activity and excitability in cortex is emerging as a promising approach for facilitating rehabilitative interventions after brain damage, including stroke. In this study, we investigated whether CS-induced functional improvements are linked with synaptic plasticity in peri-infarct cortex and vary with the severity of impairments. Adult rats that were proficient in skilled reaching received subtotal unilateral ischemic sensorimotor cortex (SMC) lesions and implantation of chronic epidural electrodes over remaining motor cortex. Based on the initial magnitude of reaching deficits, rats were divided into severely and moderately impaired subgroups. Beginning two weeks post-surgery, rats received 100 Hz cathodal CS at 50% of movement thresholds or no-stimulation control procedures (NoCS) during 18 days of rehabilitative training on a reaching task. Stereological electron microscopy methods were used to quantify axodendritic synapse subtypes in motor cortical layer V underlying the electrode. In moderately, but not severely impaired rats, CS significantly enhanced recovery of reaching success. Sensitive movement analyses revealed that CS partially normalized reaching movements in both impairment subgroups compared to NoCS. Additionally, both CS subgroups had significantly greater density of axodendritic synapses and moderately impaired CS rats had increases in presumed efficacious synapse subtypes (perforated and multiple synapses) in stimulated cortex compared to NoCS. Synaptic density was positively correlated with post-rehabilitation reaching success. In addition to providing further support that CS can promote functional recovery, these findings suggest that CS-induced functional improvements may be mediated by synaptic structural plasticity in stimulated cortex.
Subject(s)
Brain Damage, Chronic/rehabilitation , Electric Stimulation Therapy/methods , Motor Cortex/physiopathology , Neuronal Plasticity , Recovery of Function , Stroke Rehabilitation , Animals , Behavior, Animal , Brain Damage, Chronic/physiopathology , Dendrites/ultrastructure , Electrodes, Implanted , Forelimb/innervation , Forelimb/physiopathology , Male , Microelectrodes , Motor Cortex/ultrastructure , Motor Skills Disorders/etiology , Motor Skills Disorders/rehabilitation , Movement , Neural Pathways , Presynaptic Terminals/ultrastructure , Rats , Rats, Long-Evans , Severity of Illness Index , Stroke/physiopathology , Synaptic Transmission , Treatment OutcomeABSTRACT
Golgi-Cox-impregnated pyramidal neurons of layer five motor cortical area were investigated in control, binaural ear-occluded control, undernourished and binaural ear-occluded undernourished Wistar rats of 12, 20 and 30 days of age. In neonatally undernourished, binaural ear-occluded-undernourished and partly in ear-occluded-control subjects, there were significant reductions in both the number and extent of the distal part of the dendritic branches of motor pyramids compared to their controls. Moreover, minimal effects on perikarya measurements were observed. These findings suggest that neonatal undernutrition and the concurrent reduction of auditory cues affect dendritic arbor development and possibly the convergence of the auditory experience upon motor pyramids and may interfere with the neocortical modulation of postural and movements activities.
Subject(s)
Animals, Newborn/physiology , Ear/physiology , Food Deprivation/physiology , Hearing/physiology , Motor Cortex/growth & development , Acoustic Stimulation , Animals , Cortical Synchronization , Dendrites/physiology , Dendrites/ultrastructure , Female , Male , Motor Cortex/ultrastructure , Rats , Rats, WistarABSTRACT
Intra- or juxta-columnar connections of pyramidal neurons to corticospinal neurons in rat motorsensory cortices were examined with brain slices by combining intracellular staining with Golgi-like retrograde labeling of corticospinal neurons. Of 108 intracellularly labeled pyramidal neurons, 27 neurons were selected for morphological analysis by successful staining of their axonal arborizations and sufficient retrograde labeling of corticospinal neurons. Many varicosities of local axon collaterals of each pyramidal neuron were closely apposed to the dendrites of corticospinal neurons, suggesting the convergent projections of layer II-VI pyramidal neurons to corticospinal neurons. Particularly, the varicosities of a layer IV star-pyramidal neuron made two- to three-fold more appositions to the dendrites of corticospinal neurons than those of a pyramidal neuron in the other layers. Fifteen appositions were examined electron-microscopically and 60% of them made asymmetric axospinous synapses. The present results together with those of the preceding report suggest that thalamic inputs are conveyed to corticospinal neurons preferentially via layer IV star-pyramidal neurons with phasic response properties, and thereby might contribute to the initiation or switching of movement. In contrast, inputs with tonic response properties from the other layers seem to be integrated in corticospinal neurons, and might be useful in maintaining the activity of corticospinal neurons.
Subject(s)
Lysine/analogs & derivatives , Motor Cortex/physiology , Neural Pathways/physiology , Pyramidal Cells/physiology , Pyramidal Tracts/physiology , Somatosensory Cortex/physiology , Action Potentials/physiology , Animals , Cell Shape/physiology , Dendrites/physiology , Dendrites/ultrastructure , Immunohistochemistry , In Vitro Techniques , Male , Microscopy, Electron, Transmission , Motor Cortex/ultrastructure , Neural Pathways/ultrastructure , Presynaptic Terminals/physiology , Presynaptic Terminals/ultrastructure , Pyramidal Cells/ultrastructure , Pyramidal Tracts/ultrastructure , Rats , Rats, Wistar , Somatosensory Cortex/ultrastructure , Synaptic Membranes/physiology , Synaptic Membranes/ultrastructure , Synaptic Transmission/physiology , Thalamus/physiologyABSTRACT
Dendritic geometry has been shown to be a critical determinant of information processing and neuronal computation. However, it is not known whether cortical projection neurons that target different subcortical nuclei have distinct dendritic morphologies. In this study, fast blue retrograde tracing in combination with intracellular Lucifer yellow injection and diaminobenzidine (DAB) photoconversion in fixed slices was used to study the morphological features of corticospinal, corticostriatal, and corticothalamic neurons in layer V of rat motor cortex. Marked differences in the distribution of soma, somal size, and dendritic profiles were found among the three groups of pyramidal neurons. Corticospinal neurons were large, were located in deep layer V, and had the most expansive dendritic fields. The apical dendrites of corticospinal pyramidal neurons were thick, spiny, and branched. In contrast, nearly all corticostriatal neurons were small cells located in superficial layer V. Their apical dendritic shafts were significantly more slender, though spiny like those of corticospinal neurons. Corticothalamic neurons, which were located in superficial layer V and in layer VI, had small or medium-sized soma, slender apical dendritic shafts, and dendrites that were largely spine free. This study indicates that, in layer V of rat motor cortex, each population of projection neurons has a unique somatodendritic morphology and suggests that distinct modes of cortical information processing are operative in corticospinal, corticostriatal, and corticothalamic neurons.
Subject(s)
Dendrites/ultrastructure , Motor Cortex/cytology , Motor Cortex/physiology , Pyramidal Cells/physiology , Pyramidal Cells/ultrastructure , Rats/anatomy & histology , Synaptic Transmission , Animals , Corpus Striatum/physiology , Fluorescent Dyes , In Vitro Techniques , Isoquinolines , Male , Motor Cortex/ultrastructure , Pyramidal Tracts/cytology , Pyramidal Tracts/ultrastructure , Rats, Sprague-Dawley , Thalamus/physiologyABSTRACT
The ventrolateral part of the parafascicular thalamic nucleus (PF), which is considered to take part in the control mechanism of orofacial motor functions, receives projection fibers not only from the dorsolateral part of the substantia nigra pars reticulata (SNr) but also from the ventral part of the reticular thalamic nucleus (RT) [Tsumori et al., Brain Res. 858 (2000) 429]. In order to better understand the influence of these fibers upon the PF projection neurons, the morphology, synaptology and chemical nature of them were examined in the present study. After ipsilateral injections of Phaseolus vulgaris-leucoagglutinin (PHA-L) into the dorsolateral part of the SNr and biotinylated dextran amine (BDA) into the ventral part of the RT, overlapping distributions of PHA-L-labeled SNr fibers and BDA-labeled RT fibers were seen in the ventrolateral part of the PF. At the electron microscopic level, the SNr terminals made synapses predominantly with the medium to small dendrites and far less frequently with the somata and large dendrites, whereas approximately half of the RT terminals made synapses with the somata and large dendrites and the rest did with the medium to small dendrites of PF neurons. Some of single dendritic as well as single somatic profiles received convergent synaptic inputs from both sets of terminals. These terminals were packed with pleomorphic synaptic vesicles and formed symmetrical synapses. After combined injections of PHA-L into the dorsolateral part of the SNr, BDA into the ventral part of the RT and wheat germ agglutinin-horseradish peroxidase (WGA-HRP) into the ventrolateral part of the striatum or into the rostroventral part of the lateral agranular cortex, WGA-HRP-labeled neurons were embedded in the plexus of PHA-L- and BDA-labeled axon terminals within the ventrolateral part of the PF, where the PHA-L- and/or BDA-labeled terminals were in synaptic contact with single somatic and dendritic profiles of the WGA-HRP-labeled neurons. Furthermore, the SNr and RT axon terminals were revealed to be immunoreactive for gamma-aminobutyric acid (GABA), by using the anterograde BDA tracing technique combined with immunohistochemistry for GABA. The present data suggest that GABAergic SNr and RT fibers may exert different inhibitory influences on the PF neurons for regulating the thalamic outflow from the PF to the cerebral cortex and/or striatum in the control of orofacial movements.
Subject(s)
Intralaminar Thalamic Nuclei/ultrastructure , Neural Inhibition/physiology , Neural Pathways/ultrastructure , Presynaptic Terminals/ultrastructure , Substantia Nigra/ultrastructure , gamma-Aminobutyric Acid/metabolism , Animals , Corpus Striatum/metabolism , Corpus Striatum/ultrastructure , Dendrites/metabolism , Dendrites/ultrastructure , Immunohistochemistry , Intralaminar Thalamic Nuclei/metabolism , Male , Microscopy, Electron , Motor Cortex/metabolism , Motor Cortex/ultrastructure , Neural Pathways/metabolism , Presynaptic Terminals/metabolism , Rats , Rats, Wistar , Substantia Nigra/metabolism , Synaptic Transmission/physiologyABSTRACT
We reported previously that 3 months following a unilateral lesion of the nigrostriatal pathway with 6-hydroxydopamine (6-OHDA), there was a decrease in the extracellular level of striatal glutamate as determined by in vivo microdialysis. This resulted in an accumulation or increase in the density of nerve terminal glutamate immunolabeling (Meshul et al., 1999). We also reported on blockade of dopamine D-2 receptors with haloperidol resulting in ultrastructural changes within the striatum consistent with increased functioning of the glutamatergic corticostriatal pathway (Meshul and Tan 1994). We hypothesized that administration of haloperidol to 6-OHDA-lesioned rats may be capable of activating the corticostriatal pathway and thereby counteracting the effects of the unilateral nigrostriatal lesion. Striatal glutamatergic function was evaluated using electron microscopy and quantitative glutamate immunocytochemistry. Starting 1 month after a unilateral lesion of the nigrostriatal pathway with 6-OHDA, haloperidol (0.5 mg/kg/d) was administered for the next 2 months. Within the dorsolateral caudate nucleus, the main area of innervation from the motor cortex, haloperidol blocked the 6-OHDA-induced increase in the density of nerve terminal glutamate immunolabeling. Within all three experimental groups (6-OHDA, haloperidol, 6-OHDA/haloperidol) there was an increase in the mean percentage of striatal asymmetrical synapses containing a perforated postsynaptic density. In addition, haloperidol treatment resulted in a reduction in the number of apomorphine-induced contralateral rotations in unilaterally 6-OHDA lesioned rats. The data suggests that the decrease in striatal glutamatergic function 3 months following a unilateral 6-OHDA lesion can be reversed by daily haloperidol treatment. This finding is discussed in terms of current therapy for Parkinson's disease. Synapse 36:129-142, 2000. Published 2000 Wiley-Liss, Inc.
Subject(s)
Dopamine Antagonists/pharmacology , Dopamine Antagonists/therapeutic use , Glutamic Acid/metabolism , Haloperidol/pharmacology , Haloperidol/therapeutic use , Neostriatum/drug effects , Neostriatum/metabolism , Presynaptic Terminals/drug effects , Presynaptic Terminals/metabolism , Animals , Apomorphine/pharmacology , Denervation , Dopamine Agonists/pharmacology , Immunohistochemistry , Male , Motor Cortex/drug effects , Motor Cortex/metabolism , Motor Cortex/ultrastructure , Neostriatum/ultrastructure , Nerve Degeneration/chemically induced , Neural Pathways/cytology , Neural Pathways/drug effects , Neural Pathways/metabolism , Oxidopamine , Parkinsonian Disorders/drug therapy , Parkinsonian Disorders/physiopathology , Presynaptic Terminals/ultrastructure , Rats , Rats, Sprague-Dawley , Time FactorsSubject(s)
Brain/drug effects , Gangliosides/pharmacology , Learning/drug effects , Locomotion/drug effects , Signal Transduction/drug effects , Adenylyl Cyclases/metabolism , Animals , Brain/enzymology , Brain/physiology , Carbachol/pharmacology , Cholinergic Agonists/pharmacology , Conditioning, Operant/drug effects , Hippocampus/drug effects , Hippocampus/enzymology , Hippocampus/physiology , Male , Motor Cortex/drug effects , Motor Cortex/enzymology , Motor Cortex/physiology , Motor Cortex/ultrastructure , Rats , Rats, Wistar , Somatosensory Cortex/drug effects , Somatosensory Cortex/enzymology , Somatosensory Cortex/physiology , Somatosensory Cortex/ultrastructure , Synaptosomes/drug effects , Synaptosomes/enzymologyABSTRACT
As it was revealed by electron microscopic study, phenazepam (benzodiazepine representative) administered to rats in acute toxic dose caused astrocyte processes swelling mainly in reticular portion of substantia nigra and cerebral areas with high density of GABAergic synapses in initial intoxication stage (one hour after the drug introduction). Following 24 hrs changes of axodendritic and axo-somatic synapse postsynaptic part were detected similar to those of myoneural synapses induced by anti-cholinesterase agents, which allows to suggest the contribution of the increased intracellular calcium ions content. Specific benzodiazepine receptor agonist-flumazenil, introduced 30 min later than phenazepam leaded to the decrease of ultrastructural changes in postsynaptic part of the synapses in reticular zone of substantia nigra. Thus toxic effect of drugs that cause prolonged activity of peripheral and central synapses postsynaptic part results in the alike ultrastructural changes irrespective of their mediator type and is likely to be connected with the growth of intracellular calcium concentration.