Time-/dose- series transcriptome data analysis and traditional Chinese medicine treatment of pneumoconiosis.

Pneumoconiosis' pathogenesis is still unclear and specific drugs for its treatment are lacking. Analysis of series transcriptome data often uses a single comparison method, and there are few reports on using such data to predict the treatment of pneumoconiosis with traditional Chinese medicine (TCM). Here, we proposed a new method for analyzing series transcriptomic data, series difference analysis (SDA), and applied it to pneumoconiosis. By comparison with 5 gene sets including existing pneumoconiosis-related genes and gene set functional enrichment analysis, we demonstrated that the new method was not inferior to two existing traditional analysis methods. Furthermore, based on the TCM-drug target interaction network, we predicted the TCM corresponding to the common pneumoconiosis-related genes obtained by multiple methods, and combined them with the high-frequency TCM for its treatment obtained through literature mining to form a new TCM formula for it. After feeding it to pneumoconiosis modeling mice for two months, compared with the untreated group, the coat color, mental state and tissue sections of the mice in the treated group were markedly improved, indicating that the new TCM formula has a certain efficacy. Our study provides new insights into method development for series transcriptomic data analysis and treatment of pneumoconiosis.

Pulmonary rehabilitation for pneumoconiosis: protocol for a systematic review and meta-analysis.

INTRODUCTION: Pneumoconiosis is characterised by diffuse fibrosis in lung tissue, and its incidence is on the rise. At present, there are limited therapeutic options for pneumoconiosis. Pulmonary rehabilitation (PR) has been widely used to treat pneumoconiosis，however, there is limited evidence concerning its efficacy. Therefore, we plan to conduct a systematic review to investigate the efficacy and safety of PR for pneumoconiosis. METHODS AND ANALYSIS: The following databases will be searched from their inception to 1 April 2019: PubMed, Embase, The Cochrane Library, Web of Science, Chinese Biomedical Literature Database, China National Knowledge Infrastructure, Chongqing VIP and Wanfang Data. Randomised controlled trials of PR for pneumoconiosis will be included. Primary outcomes will include 6 min walk distance and St. George's Respiratory Questionnaire. Study selection, extraction of data and assessment of study quality each will be independently undertaken. Statistical analysis will be conducted using Review Manager software. ETHICS AND DISSEMINATION: This systematic review will provide up-to-date information on PR for pneumoconiosis. The review does not require ethical approval and will be disseminated electronically through a peer-reviewed publication or conference presentations. PROSPERO REGISTRATION NUMBER: CRD42018095266.

[Introduction to Professor Ma Guiqin's experience in treatment of occupational pneumoconiosis].

Occupational pneumoconiosis refers to a group of occupational pulmonary diseases,which is mainly characterized by diffuse fibrosis of lung tissue caused by inhalation of different pathogenic productive dust and retention in the lungs during occupational activities for a long period of time. According to the classification and catalogue of occupational diseases in China,it is mainly classified into 12 categories,such as silicosis,coal worker's pneumoconiosis,asbestos lung. At present,it is the most serious and most common occupational disease in China. The pathogenesis of pneumoconiosis is not clear,and there is still no effective therapy. In the past three years,it is found that traditional Chinese medicine is effective in the treatment of pneumoconiosis. Through collection of medical records,the regularity of the prescriptions used in the treatment of pneumoconiosis were analyzed and summarized. According to the traditional Chinese medicine theory,the clinical characteristics of recurrent cough,phlegm and asthma in patients with pneumoconiosis could be included in the categories of cough,wheezing,lung distension. Professor Ma Guiqin believes that the basic pathogenesis of pneumoconiosis is depression of the atmosphere,deficiency of the kidney,internal obstruction of phlegm,and adverse cardinality. According to its pathogenesis,the commonly used prescriptions,namely Shengxian Decoction,Jinshui Liujun Jian,Xiaochaihu Decoction,Sini Powder,Guomin Jian and syndrome differentiation-based modified medicines were expounded. In addition, " throat is the gateway to the lung and stomach",Professor Ma also believes that the prevention of pneumoconiosis should start with the throat,so as to curb the exacerbation or recurrence of the disease. Through clinical observation,if traditional Chinese medicine is properly used,and pathogenesis and syndrome differentiation-based treatment is provided,traditional Chinese medicine can relieve the clinical symptoms,and improve the exercise tolerance and the quality of life of patients with pneumoconiosis.

[Clinical study on traditional Chinese medicine syndrome differentiation and treatment of pneumoconiosis].

Objective: To explore the clinical effect of traditional Chinese medicine (TCM) syndrome differentiation in the treatment of pneumoconiosis. Methods: We selected 160 pneumoconiosis patients who were hospitalized in our hospital from July 2015 to July 2016. 102 patients were divided into experimental groups, and the other 58 patients were assigned to the control group. These patients were randomly assigned. The control group was given routine treatment, while the experimental group received conventional treatment combined with TCM syndrome differentiation. We rated patients' quality of life using the SF-36 Quality of Life Scale. C-reactive protein levels in both groups before and after treatment were detected by ELISA. Furthermore, we detected the expression levels of IL-6 mRNA and TGF-ß mRNA in serum before and after treatment by RT-PCR.The analysis was performed with SPSS19.0 software. The normal distribution measurement data was expressed by Mean±SD, and the t-test was used for statistical analysis. Non-normal distribution measurement data were expressed as median(M) and interquartile range (P(25), P(75)), and non-parametric test was used for statistical analysis. Results: Compared with the control group, the readmission rate in the experimental group decreased within 3 months; The SF-36 quality of life score results of the two groups showed a statistically significant difference in BP, VT and RE (P<0.05);The levelofCRPdecreasedsignificantlyafter treatment in bothgroups,and thedifferencewasstatistically significant (P<0.05).Comparedwith thecontrolgroup, the levelsofIL-6mRNAandTGF-ß1mRNA alsodecreased (P<0.05). Conclusion: TCM syndromedifferentiation and treatmentofpneumoconiosis can effectively improve the patient's condition and life quality. We predict that it may inhibit the progression of pulmonary fibrosis in pneumoconiosis.

[Clinical abservations of pulmonary surfactant' s autologous transfusion in massive lung lavage].

Objective: Investigate the pulmonary surfactant autotransfusion effect on the recovery of respiratory function in patients with whole lung lavage, to provide theoretical basis for the clinical application. Methods: We taken 30 patients of pneumoconiosis treated by whole lung lavage as the subjects. We extracted the pulmonary surfactant from lavage fluid, after single postoperative lung lavage for the first time; after one weeks when the second times of lung lavage were performed to the other side of the lung of patients, we put PS into the right side. We taken the patients the second times of lung lavage who were put PS into the right side as returning group, the first times of lung lavage who were not put PS into as on returning group. We observed indi-cators, such as expiratory resistance, respiratory work, lung compliance, airway pressure, PO(2), the pulmonary function recovery time and other indicators, comparing with the changes of pulmonary function before lung la-vage for the first time and at 0、60、90、120 min after the pulmonary surfactant autotransfusion. Results: Com-pared with the no returning group, the expiratory resistance of the returning group decreased significantly at 90 min、120 min after the pulmonary surfactant autotransfusion; the respiratory work and airway pressure of the re-turning group decreased significantly at 60、90、120 min after the pulmonary surfactant autotransfusion, there was statistically significant in the difference between different groups (P<0.05, P<0.01). Compared with the no returning group, the lung compliance and the PO(2) of the returning group increased significantly at 60 min、90 min、120 min after the pulmonary surfactant autotransfusion, there was statistically significant in the difference between different groups (P<0.05, P<0.01). The lung function recovery time of returning group was (155.7 ± 35.2) min, the lung function recovery time of no returning group was (183.71±41.81) min, there was statistical-ly significant in the difference between different groups (P<0.05). Compared with the no returning, there were not statistically significant in the difference of the Heart rate、the systolic blood pressure and the diastolic blood pressure about the returning at 60、90、120 min after the pulmonary surfactant autotransfusion.There was no ad-verse reactions such as pulmonary infection, pulmonary infection and so on. Conclusion: The pulmonary surfac-tant autotransfusion may reduce expiratory resistance, work of breathing, airway pressure; improve lung compliance, alveolar ventilation function; increase oxygen partial pressure and decrease the surgery recovery time in patients with pneumoconiosis.

[Randomized Controlled Clinical Trials for Acupuncture Treatment of Pneumoconiosis].

OBJECTIVE: To observe the clinical therapeutic effect of acupuncture therapy in improving pulmonary functions and clinical symptoms of pneumoconiosis patients. METHODS: A total of 120 pneumoconiosis patients were randomly divided into acupuncture group (n=59) and control group (n = 61). The patients of the control group were ordered to take Acetylcysteine capsule (200 mg/time, t.i.d.) for 12 consecutive weeks and the patients of the acupuncture group received both acupuncture (twice a week) and Acetylcysteine capsule for 12 consecutive weeks. The used acupoints were Baihui (GV 20), Tanzhong (CV 17), Zhongwan (CV 12), Qihai (CV 6), and bilateral Kongzui (LU 6), Taiyuan (LU 9), Yuji (LU 10), Zusanli (ST 36), Fenglong (ST 40), Sanyinjiao (SP 6), Feishu (BL 13), Xinshu (BL 15), Ganshu (BL 18), Pishu (BL 20), Shenshu (BL 23) and Geshu (BL 17) and stimulated by needle-twirling reducing or reinforcing techniques. The pulmonary functions including spirometric forced vital capacity(FVC) and forced expiratory volume in one second (FEV 1), FEV 1/FVC, and diffusion capacity for carbon monoxide (DLCO) were detected by using a Medgraphics Cardio 2 Combined VO 2/ECG Exercise System. The severity of cough and shortness of breath (SOB) was detected by Visual Analog Scale (VAS), and the patient's health status was assessed using Chronic Obstructive Pulmonary Disease Assessment Test (CAT score, 0-40 points). RESULTS: Compared with pretreatment in the same one group, FE1 level and FEV 1/FVC after 12 weeks' treatment were obviously increased in both the control and acupuncture groups (P<0.05), while the CAT scores at the 12th week, the cough and SOB scores at the 6th, 12th and 16th week after the treatment were decreased notably in both groups (P<0.05), and the cough and SOB scores of the acupuncture group were significantly lower than those of the control group (P<0.05). No significant changes were found in the FVC and DLCO levels in both groups (P>0.05). CONCLUSION: Acupuncture therapy can improve symptoms of cough, shortness of breath, as well as pulmonary functions in pneumoconiosis patients.

[Effectiveness of climatic health resort complex treatment of patients with pneumoconiosis and dust-induced bronchitis using underwater massage shower]. / Effektivnost' klimaticheskogo sanatorno-kurortnogo lecheniia bol'nykh pnevmokoniozom i pylevym bronkhitom s vkliucheniem v lechebny kompleks protsedur podvodnogo dusha-massazha.

The article provides the results of studies of 167 cases affected with occupationally-related pneumoconiosis and dust bronchitis. 117 patients were treated with complex resort therapy including under-water massage, and 50 patients were given the same complex therapy without shower massage. The effectiveness of the therapeutic techniques were evaluated basing on both objective and subjective criteria, including supplementary laboratory and functional techniques. The results were positive in both groups, but in the major group, the favourable shifts were more expressed but statistically were not reliable. Thus, the underwater shower massage may be regarded as a positive but not decisive factor in the complex resort treatment of occupational diseases of lungs.

[The use of hyperbaric oxygenation for the treatment of patients with pneumoconiosis and pulmonary heart failure]. / Primenenie giperbaricheskoi oksigenatsii dlia lecheniia bol'nykh pnevmokoniozom s legochno-serdechnoi nedostatochnost'iu.

Examination of 76 patients with pneumoconiosis (stage I) revealed that hyperbaric oxygenation is increasing the force of cardiac contractions, reduction of the general peripheral resistance and reduction of the lesser circulation hypertension. The therapeutic action of hyperbaric oxygenation is related to its ability to remove the sequels of oxygen.

[Actual problems of the treatment of the main forms of occupational diseases]. / Aktual'nye voprosy lecheniia osnovnykh form professional'nykh zabolevanii.

The article presents data on the treatment of dust induced diseases of the lungs, occupational bronchial pathology, lead poisoning, allergic skin diseases, occupational hypoacusis and occupational NS disorders. The treatment techniques included relatively new therapies of which non-medicinal techniques were preferred, such as needle therapy, entherosorption, phytotherapy, manual therapy, vacuum massage, etc.

Explanatory models of black lung: understanding the health-related behavior of Appalachian coal miners.

Many retired coal miners who are eligible for care in a black lung treatment center at little or no cost to themselves do not enter into available programs or discontinue soon after beginning therapy. Reasons for this behavior are related to the prevalent beliefs among Appalachians concerning the course of black lung and the appropriate treatment for it. The miners' health beliefs are clearly at odds with those of the health care providers who work in the centers. Using the concept of explanatory model, popular and professional health cultures are analyzed, focusing on course of disease, sick role, appropriate treatment, and expected outcome. Differences in explanatory models are discussed with regard to implications for the organization and delivery of care to retired coal miners with black lung.