ABSTRACT
Drug-loaded electrospun fibers have attracted increasing attention as a promising wound dressing material due to their capability of preventing from infections and inflammation and maintaining an appropriate environment for wound healing. In this study, polylactic acid (PLA), which is widely used in wound management, was chosen as electrospinnable polymer. A triterpene extract (TE) from the outer bark of birch known for its anti-inflammatory, antiviral, antibacterial, and wound healing effects was chosen to produce TE-loaded PLA electrospun fibers for wound dressing. A binary solvent system of dichloromethane (DCM) and dimethyl sulfoxide (DMSO) was employed, and the ratio of the solvents was optimized for preparing smooth and uniform fibers. The morphology of TE-loaded PLA electrospun fibers was investigated by scanning electron microscopy (SEM). The entrapment of TE in PLA fibers was confirmed by confocal laser scanning microscopy (CLSM). Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) were used to analyze the solid state of TE in PLA fibers. The release behavior of TE was assayed by a shaking flask method for a period of 96 h. The results revealed that TE-loaded electrospun PLA microfibers could be reliably prepared and are promising future candidates in wound therapy.
Subject(s)
Bandages , Betula/chemistry , Nanofibers/chemistry , Plant Bark/chemistry , Polyesters/chemical synthesis , Triterpenes/chemical synthesis , Anti-Bacterial Agents/analysis , Anti-Bacterial Agents/chemical synthesis , Chemistry, Pharmaceutical/methods , Nanofibers/analysis , Plant Extracts/analysis , Plant Extracts/chemical synthesis , Polyesters/analysis , Triterpenes/analysisABSTRACT
PURPOSE: Focused ultrasound (FUS) is a noninvasive method to produce thermal and mechanical destruction along with an immune-stimulatory effect against cancer. However, FUS ablation alone appears insufficient to generate consistent antitumor immunity. In this study, a multifunctional nanoparticle was designed to boost FUS-induced immune effects and achieve systemic, long-lasting antitumor immunity, along with imaging and thermal enhancement. MATERIALS AND METHODS: PEGylated PLGA nanoparticles encapsulating astragalus polysaccharides (APS) and gold nanorods (AuNRs) were constructed by a simple double emulsion method, characterized, and tested for cytotoxicity. The abilities of PA imaging and thermal-synergetic ablation efficiency were analyzed in vitro and in vivo. The immune-synergistic effect on dendritic cell (DC) differentiation in vitro and the immune response in vivo were also evaluated. RESULTS: The obtained APS/AuNR/PLGA-PEG nanoparticles have an average diameter of 255.00±0.1717 nm and an APS-loading efficiency of 54.89±2.07%, demonstrating their PA imaging capability and high biocompatibility both in vitro and in vivo. In addition, the as-prepared nanoparticles achieved a higher necrosis cell rate and induced apoptosis rate in an in vitro cell suspension assay, greater necrosis area and decreased energy efficiency factor (EEF) in an in vivo rabbit liver assay, and remarkable thermal-synergic performance. In particular, the nanoparticles upregulated the expression of MHC-II, CD80 and CD86 on cocultured DCs in vitro, followed by declining phagocytic function and enhanced interleukin (IL)-12 and interferon (INF)-γ production. Furthermore, they boosted the production of tumor necrosis factor (TNF)-α, IFN-γ, IL-4, IL-10, and IgG1 (P< 0.001) but not IgG2a. Immune promotion peaked on day 3 after FUS in vivo. CONCLUSION: The multifunctional APS/AuNR/PLGA-PEG nanoparticles can serve as an excellent synergistic agent for FUS therapy, facilitating real-time imaging, promoting thermal ablation effects, and boosting FUS-induced immune effects, which have the potential to be used for further clinical FUS treatment.
Subject(s)
Astragalus Plant/chemistry , Breast Neoplasms/therapy , Gold/chemistry , Multifunctional Nanoparticles/chemistry , Nanotubes/chemistry , Polysaccharides/chemistry , Ultrasonic Therapy , Animals , Antigens, CD/metabolism , Apoptosis , Cell Death , Cell Differentiation , Cell Line, Tumor , Cell Proliferation , Cytokines/metabolism , Dendritic Cells/cytology , Female , Human Umbilical Vein Endothelial Cells/metabolism , Humans , Immunoglobulin G/metabolism , Lymphocytes, Tumor-Infiltrating/immunology , Mice, Inbred BALB C , Phagocytosis , Photoacoustic Techniques , Polyesters/chemical synthesis , Polyesters/chemistry , Polyethylene Glycols/chemical synthesis , Polyethylene Glycols/chemistry , Rabbits , Theranostic Nanomedicine , Vascular Endothelial Growth Factor A/metabolismABSTRACT
To achieve improved drug delivery efficiency to hepatocellular carcinoma (HCC), biodegradable poly (ethylene glycol)-poly (lactic-co-glycolic acid) (PEG-PLGA) nanoparticles (NP), surface-modified with SP94 peptide, were designed for the efficient delivery of cryptotanshinone to the tumor for the treatment of HCC. Cryptotanshinone NP and SP94-NP were prepared by using nanoprecipitation. The physicochemical and pharmaceutical properties of the NP and SP94-NP were characterized, and the release kinetics suggested that both NP and SP94-NP provided continuous, slow release of cryptotanshinone for 48 h. The in vitro cellular experiment demonstrated that SP94-NP significantly enhanced the cellular uptake of cryptotanshinone and induced high cytotoxicity and cellular apoptosis of hepatocellular carcinoma (HepG2) cells. The in vivo detecting results of targeting effect using the Cy5.5 probe evidenced that SP94-NP showed an accumulation in tumor more efficiently than that of unconjugated ones. Meanwhile, SP94-NP exhibited the smallest tumor size than other groups and showed no toxicity to body. The results of this study provide a promising nanoplatform for the targeting of HCC.
Subject(s)
Carcinoma, Hepatocellular/drug therapy , Liver Neoplasms/drug therapy , Nanoparticles/administration & dosage , Peptide Fragments/administration & dosage , Phenanthrenes/administration & dosage , Polyesters/administration & dosage , Polyethylene Glycols/administration & dosage , Animals , Carcinoma, Hepatocellular/metabolism , Carcinoma, Hepatocellular/pathology , Cell Survival/drug effects , Cell Survival/physiology , Dose-Response Relationship, Drug , Drug Delivery Systems/methods , Drugs, Chinese Herbal/administration & dosage , Drugs, Chinese Herbal/chemical synthesis , Drugs, Chinese Herbal/metabolism , Female , Hep G2 Cells , Humans , Liver Neoplasms/metabolism , Liver Neoplasms/pathology , Mice , Mice, Inbred BALB C , Mice, Nude , Nanoparticles/chemistry , Nanoparticles/metabolism , Peptide Fragments/chemical synthesis , Peptide Fragments/metabolism , Phenanthrenes/chemical synthesis , Phenanthrenes/metabolism , Polyesters/chemical synthesis , Polyesters/metabolism , Polyethylene Glycols/chemical synthesis , Polyethylene Glycols/metabolismABSTRACT
The aim of the present research was to investigate the feasibility of developing polylactide-polycaprolactone-polyethylene glycol-polycaprolactone-polylactide (PLA-PCL-PEG-PCL-PLA) based micelles to improve ocular permeability of dexamethasone (DEX). PLA-PCL-PEG-PCL-PLA copolymers were synthesized by a ring-opening polymerization method. DEX was loaded into the developed copolymers. The DEX-loaded micelles were characterized using transmission electron microscopy (TEM) and dynamic light scattering (DLS) methods. Cytotoxicity of the micelles obtained was investigated on L929 cell line. Cellular uptake was followed by fluorescence microscopy and flow cytometry analyses. The release behavior of DEX from the micelles as well as the drug release kinetics was studied. Corneal permeability was also evaluated using an ex vivo bovine model. The pentablock copolymers were successfully synthesized. The TEM results verified the formation of spherical micelles, the sizes of which was approximately 65 nm. The micelles exhibited suitable compatibility on L929 cells. The release profile showed an initial burst release phase followed by a sustained release phase, the kinetic of which was close to the Weibull's distribution model. The micelles showed higher corneal permeability in comparison to a marketed DEX eye drop. Taken together, the results indicated that the PLA-PCL-PEG-PCL-PLA micelles could be appropriate candidates for the ocular delivery of DEX, and probably other hydrophobic drugs.
Subject(s)
Cornea/metabolism , Dexamethasone/chemical synthesis , Drug Development/methods , Micelles , Polyesters/chemical synthesis , Polyethylene Glycols/chemical synthesis , Animals , Anti-Inflammatory Agents/chemical synthesis , Anti-Inflammatory Agents/pharmacokinetics , Cattle , Cell Line , Cornea/drug effects , Dexamethasone/pharmacokinetics , Drug Evaluation, Preclinical/methods , Mice , Organ Culture Techniques , Permeability/drug effects , Polyesters/pharmacokinetics , Polyethylene Glycols/pharmacokineticsABSTRACT
Phosphorus-containing flame retardants synthesized from renewable resources have had a lot of impact in recent years. This article outlines the synthesis, characterization and evaluation of these compounds in polyesters and epoxy resins. The different approaches used in producing biobased flame retardant polyesters and epoxy resins are reported. While for the polyesters biomass derived compounds usually are phosphorylated and melt blended with the polymer, biobased flame retardants for epoxy resins are directly incorporated into the polymer structure by a using a phosphorylated biobased monomer or curing agent. Evaluating the efficiency of the flame retardant composites is done by discussing results obtained from UL94 vertical burning, limiting oxygen index (LOI) and cone calorimetry tests. The review ends with an outlook on future development trends of biobased flame retardant systems for polyesters and epoxy resins.
Subject(s)
Epoxy Resins/chemical synthesis , Flame Retardants/chemical synthesis , Lignin/chemistry , Phosphorus/chemistry , Polyesters/chemical synthesis , Benzaldehydes/chemistry , Biomass , Epoxy Resins/chemistry , Fermentation , Humans , Polyesters/chemistry , Propylene Glycol/chemistryABSTRACT
Large amounts of agricultural wastes are rich in pectins that, in many cases, disrupt the processing of food residues due to gelation. Despite pectins being a promising sustainable feedstock for bio-based chemical production, the current pathways to produce platform molecules from this polysaccharide are hazardous and entail the use of strong acids. The present work describes a sequence of biocatalyzed reactions that involves 1) the extraction of pectin from sugar beet pulp and enzymatic recovery of galacturonic acid (GalA), followed by 2) the enzymatic oxidation of the GalA aldehyde and the recovery of galactaric acid (GA), and 3) the biocatalyzed polycondensation of GA to obtain fully bio-based polyesters carrying lateral hydroxy functionalities. The acid-free pectin extraction is optimized using enzymes and microwave technology. The conditions for enzymatic oxidation of GalA allow the separation of the GA produced by a simple centrifugation step that leads to the enzyme-catalyzed polycondensation reactions.
Subject(s)
Pectins/chemistry , Polyesters/chemistry , Polymers/chemistry , Sugar Acids/chemistry , Beta vulgaris/chemistry , Beta vulgaris/enzymology , Biocatalysis , Enzymes/metabolism , Hexuronic Acids/chemistry , Hexuronic Acids/metabolism , Models, Chemical , Molecular Structure , Polyesters/chemical synthesis , Polymers/chemical synthesis , Polysaccharides/chemistry , Polysaccharides/metabolismABSTRACT
Biodegradable polycaprolactone (PCL) has been widely applied as a scaffold material in tissue engineering. However, the PCL surface is hydrophobic and adsorbs nonspecific proteins. Some traditional antifouling modifications using hydrophilic moieties have been successful but inhibit cell adhesion, which is not ideal for tissue engineering. The PCL surface is modified with bioinspired zwitterionic poly[2-(methacryloyloxy)ethyl choline phosphate] (PMCP) via surface-initiated atom transfer radical polymerization to improve cell adhesion through the unique interaction between choline phosphate (CP, on PMCP) and phosphate choline (PC, on cell membranes). The hydrophilicity of the PCL surface is significantly enhanced after surface modification. The PCL-PMCP surface reduces nonspecific protein adsorption (e.g., up to 91.7% for bovine serum albumin) due to the zwitterionic property of PMCP. The adhesion and proliferation of bone marrow mesenchymal stem cells on the modified surface is remarkably improved, and osteogenic differentiation signs are detected, even without adding any osteogenesis-inducing supplements. Moreover, the PCL-PMCP films are more stable at the early stage of degradation. Therefore, the PMCP-functionalized PCL surface promotes cell adhesion and osteogenic differentiation, with an antifouling background, and exhibits great potential in tissue engineering.
Subject(s)
Biofouling , Cell Differentiation/drug effects , Osteogenesis/drug effects , Phosphorylcholine/analogs & derivatives , Polyesters/pharmacology , Polymethacrylic Acids/pharmacology , Tissue Engineering , Adsorption , Animals , Animals, Newborn , Biocompatible Materials/pharmacology , Cell Adhesion/drug effects , Cell Proliferation/drug effects , Cytokines/metabolism , Mesenchymal Stem Cells/cytology , Mesenchymal Stem Cells/drug effects , Phosphorylcholine/chemical synthesis , Phosphorylcholine/pharmacology , Photoelectron Spectroscopy , Polyesters/chemical synthesis , Polymethacrylic Acids/chemical synthesis , Rats, Sprague-Dawley , Surface Properties , Water/chemistryABSTRACT
Two novel phosphorus-containing copolyesters were synthesized by direct polycondensation reaction of phenyl dichlorophosphate, 1,4-succinic acid and 1,4-butanediol using stannous chloride (SnCl2) and 4-Methylbenzenesulfonic acid as catalyst, and its chemical structures were identified by 1H and 31P nuclear magnetic resonances (1H and 31P NMR). The resulting phosphorus-containing poly(butylene succinate) (PPBS) was characterized by X-ray diffraction (XRD), polarized optical microscope (POM), thermogravimetry analysis (TGA) and differential scanning calorimetry (DSC). PPBS can be as a flame retardant for commercial poly(butylene succinate) (PBS). A series of flame retardant composite materials were produced by melt-blending of PBS and PPBS. The comprehensive flame retardant property of composite materials was evaluated by limited oxygen index (LOI). While 20â¯wt % of PPBS was added into PBS resin, good flame retardant properties could be obtained. Composite materials can have much better flame retardancy (LOIâ¯=â¯30) than neat PBS resin. Thermogravimetric analysis (TGA) showed that the weight loss of PBS was decreased by the introduction of PPBS. In addition, possible flame retardancy mechanism of PPBS in composite materials was analyzed by SEM photos of char residue.
Subject(s)
Butylene Glycols/chemistry , Butylene Glycols/chemical synthesis , Flame Retardants/chemical synthesis , Polyesters/chemistry , Polymers/chemistry , Polymers/chemical synthesis , Calorimetry, Differential Scanning , Flame Retardants/analysis , Magnetic Resonance Spectroscopy , Phosphorus , Polyesters/chemical synthesis , Thermogravimetry , X-Ray DiffractionABSTRACT
PURPOSE: Artemisinin (ART) has anti-inflammatory, antimicrobial, antioxidant, anti-amyloid, and anti-malarial effects, but its application is limited due to its low water solubility and poor oral bioavailability. In this study, the bioavailability, water solubility, and anti-plasmodial property of ART were improved by PCL-PEG-PCL tri-block copolymers. METHODS: The structure of the copolymers was characterized by 1H NMR, FT-IR, DSC, and GPC techniques. ART was encapsulated within micelles by a single-step nano-precipitation method, leading to the formation of ART-loaded PCL-PEG-PCL micelles. The obtained micelles were characterized by dynamic light scattering (DLS) and atomic force microscopy (AFM). The in vivo anti-plasmodial activity of ART-loaded micelles was measured against Plasmodium berghei infected Swiss albino mice. RESULTS: The results showed that the zeta potential of ART-loaded micelles was about -8.37 mV and the average size was 91.87 nm. ART was encapsulated into PCL-PEG-PCL micelles with a loading capacity of 19.33 ± 0.015% and encapsulation efficacy of 87.21 ± 3.32%. In vivo anti-plasmodial results against P. berghei showed that multiple injections of ART-loaded micelles could prolong the circulation time and increase the therapeutic efficacy of ART. CONCLUSION: These results suggested that PCL-PEG-PCL micelles would be a potential carrier for ART for the treatment of malaria.
Subject(s)
Anti-Infective Agents/administration & dosage , Drug Carriers/administration & dosage , Malaria/drug therapy , Nanoparticles/administration & dosage , Plasmodium berghei/drug effects , Polyesters/administration & dosage , Polyethylene Glycols/administration & dosage , Animals , Anti-Infective Agents/chemical synthesis , Anti-Infective Agents/pharmacokinetics , Artemisinins/chemical synthesis , Artemisinins/pharmacokinetics , Drug Carriers/chemical synthesis , Drug Carriers/pharmacokinetics , Drug Evaluation, Preclinical/methods , Female , Malaria/metabolism , Mice , Nanoparticles/chemistry , Nanoparticles/metabolism , Plasmodium berghei/physiology , Polyesters/chemical synthesis , Polyesters/pharmacokinetics , Polyethylene Glycols/chemical synthesis , Polyethylene Glycols/pharmacokineticsABSTRACT
This study investigated the therapeutic efficiency of monomethoxy polyethylene glycol-poly(lactic-co-glycolic acid) (mPEG-PLGA) co-loaded with syringopicroside and hydroxytyrosol as a drug with effective targeting and loading capacity as well as persistent circulation in vivo. The nanoparticles were prepared using a nanoprecipitation method with mPEG-PLGA as nano-carrier co-loaded with syringopicroside and hydroxytyrosol (SH-NPs). The parameters like in vivo pharmacokinetics, biodistribution in vivo, fluorescence in vivo endomicroscopy, and cellular uptake of SH-NPs were investigated. Results showed that the total encapsulation efficiency was 32.38 ± 2.76 %. Total drug loading was 12.01 ± 0.42 %, particle size was 91.70 ± 2.11 nm, polydispersity index was 0.22 ± 0.01, and zeta potential was -24.5 ± 1.16 mV for the optimized SH-NPs. The nanoparticle morphology was characterized using transmission electron microscopy, which indicated that the particles of SH-NPs were in uniformity within the nanosize range and of spherical core shell morphology. Drug release followed Higuchi kinetics. Compared with syringopicroside and hydroxytyrosol mixture (SH), SH-NPs produced drug concentrations that persisted for a significantly longer time in plasma following second-order kinetics. The nanoparticles moved gradually into the cell, thereby increasing the quantity. ALT, AST, and MDA levels were significantly lower on exposure to SH-NPs than in controls. SH-NPs could inhibit the proliferation of HepG2.2.15 cells and could be taken up by HepG2.2.15 cells. The results confirmed that syringopicroside and hydroxytyrosol can be loaded simultaneously into mPEG-PLGA nanoparticles. Using mPEG-PLGA as nano-carrier, sustained release, high distribution in the liver, and protective effects against hepatic injury were observed in comparison to SH.
Subject(s)
Drug Carriers , Glycosides/administration & dosage , Nanoparticles/chemistry , Phenylethyl Alcohol/analogs & derivatives , Polyesters , Polyethylene Glycols , Animals , Chemical and Drug Induced Liver Injury/prevention & control , Drug Carriers/adverse effects , Drug Carriers/chemical synthesis , Drug Carriers/chemistry , Drug Carriers/pharmacokinetics , Drug Evaluation, Preclinical , Female , Hep G2 Cells , Humans , Male , Materials Testing , Mice , Particle Size , Phenylethyl Alcohol/administration & dosage , Polyesters/adverse effects , Polyesters/chemical synthesis , Polyesters/chemistry , Polyesters/pharmacokinetics , Polyethylene Glycols/adverse effects , Polyethylene Glycols/chemical synthesis , Polyethylene Glycols/chemistry , Polyethylene Glycols/pharmacokinetics , Rats , Rats, Sprague-Dawley , Tissue DistributionABSTRACT
PURPOSE: Epilepsy is a prevalent neurological disorder with a high frequency of drug resistance. While significant advancements have been made in drug delivery systems to overcome anti-epileptic drug resistance, efficacies of materials in biological systems have been poorly studied. The purpose of the study was to evaluate the anti-epileptic effects of injectable poly(epsilon-caprolactone) (PCL) microspheres for controlled release of an anticonvulsant, phenytoin (PHT), in an animal model of epilepsy. METHODS: PHT-PCL and Blank-PCL microspheres formulated using an oil-in-water (O/W) emulsion solvent evaporation method were evaluated for particle size, encapsulation efficiency, surface morphology and in-vitro drug release profile. Microspheres with the most suitable morphology and release characteristics weresubsequently injected into the hippocampus of a rat tetanus toxin model of temporal lobe epilepsy. Electrocorticography (ECoG)from the cerebral cortex were recorded for all animals. The number of seizure events, severity of seizures, and seizure duration were then compared between the two treatment groups. RESULTS: We have shown that small injections of drug-loaded microspheres are biologically tolerated and released PHT can control seizures for the expected period of time that is in accord with in-vitro release data. CONCLUSION: The study demonstrated the feasibility of polymer-based delivery systems incontrolling focal seizures.
Subject(s)
Anticonvulsants/administration & dosage , Drug Resistant Epilepsy/drug therapy , Epilepsy, Temporal Lobe/drug therapy , Phenytoin/administration & dosage , Animals , Anticonvulsants/pharmacokinetics , Cerebral Cortex/drug effects , Cerebral Cortex/physiopathology , Delayed-Action Preparations/chemical synthesis , Delayed-Action Preparations/chemistry , Disease Models, Animal , Drug Evaluation, Preclinical , Drug Resistant Epilepsy/physiopathology , Electrocorticography , Epilepsy, Temporal Lobe/physiopathology , Feasibility Studies , Hippocampus/drug effects , Hippocampus/physiopathology , Male , Microspheres , Particle Size , Phenytoin/pharmacokinetics , Polyesters/chemical synthesis , Polyesters/chemistry , Rats, Sprague-Dawley , Seizures/drug therapy , Seizures/physiopathology , Surface Properties , Tetanus Toxin , Treatment OutcomeABSTRACT
Well-designed agents for enhanced multimodal imaging have attracted great interests in recent years. In this work, we adopted a premix membrane emulsification (PME) method to prepare uniform PEGylated poly(lactic-co-glycolic acid) (PLGA) microcapsules (MCs) with superparamagnetic Fe3O4 nanoparticles (NPs) embedded in the shell (Fe3O4@PEG-PLGA MCs) for ultrasound (US)/magnetic resonance (MR) bimodal imaging. Compared to Fe3O4@PLGA MCs without PEGylation, Fe3O4@PEG-PLGA MCs could more stably and homogeneously disperse in physiological solutions. In vitro and in vivo trials demonstrated that Fe3O4@PEG-PLGA MCs (â¼3.7 µm) with very narrow size distribution (PDI=0.03) could function as efficient dual-modality contrast agents to simultaneously enhance US and MR imaging performance greatly. In vitro cell toxicity and careful histological examinations illustrated no appreciable cytotoxicity and embolism of Fe3O4@PEG-PLGA MCs to mice even at high dose. The uniform composite MCs developed here can act as clinical bimodal contrast agents to improve hybrid US/MR imaging contrast, which is promising for accurate diagnosis and real-time monitoring of difficult and complicated diseases.
Subject(s)
Capsules/chemistry , Contrast Media/chemistry , Ferrosoferric Oxide/chemistry , Magnetite Nanoparticles/chemistry , Polyesters/chemistry , Polyethylene Glycols/chemistry , Animals , Biocompatible Materials/chemistry , Biocompatible Materials/pharmacology , Cell Survival/drug effects , Contrast Media/toxicity , Liver/anatomy & histology , Liver/pathology , Magnetic Resonance Imaging , Magnetite Nanoparticles/toxicity , Magnetite Nanoparticles/ultrastructure , Mice , NIH 3T3 Cells , Polyesters/chemical synthesis , Polyethylene Glycols/chemical synthesisABSTRACT
The biodegradable poly(butylene adipate-co-terephthalate)(PBAT)/thermoplastic starch (TPS) composite has received considerable attention because of the environmental concerns raised by solid waste disposal. However, the application of PBAT/TPS blends was limited due to the poor mechanical properties originating from the incompatibility between PBAT and TPS. In this work, two approaches were developed to improve the mechanical properties of PBAT/TPS blends. One approach is to use compatibilizers, including the synthesized reactive compatibilizer - a styrene-maleic anhydride-glycidyl methacrylate (SMG) terpolymer, and the commercial compatibilizer (Joncryl-ADR-4368). The chemical structures of SMG were analyzed with (1)H NMR and FT-IR. The other approach is to use the modified PBAT (M-PBAT) to replace part of PBAT in the PBAT/TPS blends. M-PBATs with higher molecular weight were obtained via reactive extrusion of PBAT in the presence of a chain extender. The better dispersion of TPS in PBAT was observed in SEM images when using M-PBAT, leading to the higher tensile strength and elongation at break of PBAT/TPS blends. However, the elongation at break decreased in the presence of compatibilizer (SMG or 4368), though the tensile strength remained in a similar level or slightly higher. Overall, the tensile strength and the elongation at break of the resulting biodegradable PBAT/M-PBAT/TPS blends (TPS=40wt%) were above 27.0MPa and 500%, respectively, which is promising for various applications, including packaging and agricultural mulching films.
Subject(s)
Polyesters/chemistry , Solanum tuberosum/metabolism , Starch/chemistry , Epoxy Compounds/chemistry , Magnetic Resonance Spectroscopy , Maleic Anhydrides/chemistry , Methacrylates/chemistry , Microscopy, Electron, Scanning , Polyesters/chemical synthesis , Spectroscopy, Fourier Transform Infrared , Styrene/chemistry , Tensile StrengthABSTRACT
Use of single chemotherapy agents has shown some limitations in anti-tumor treatment, such as development of drug resistance, severe adverse reactions and limited regime for therapeutic use. Combination of two or more therapeutic drugs is a feasible strategy to overcome these limitations. This paper reports study of co-delivery by core-shell nanoparticles (NPs) with hydrophobic PLLA core loaded with curcumin (Cur) and hydrophilic heparin shell adsorbing Doxorubicin (DOX). Characterizations of Cur-PEA NPs, Cur-PEA/heparin NPs and DOX adsorbing into Cur-PEA/heparin NPs (DOX-Cur NPs) were also investigated by transmission electron microscope (TEM) and Malvern Zetasizer. Studies on cellular uptake of DOX-Cur NPs demonstrated that both drugs were effectively taken up by 4T1 tumor cells. Furthermore, DOX-Cur NPs suppressed 4T1 tumor cells growth more efficiently than either DOX or Cur alone at the same concentrations, as measured by flow cytometry (FCM). We found out that intravenous injection of DOX-Cur NPs efficiently inhibited growth of subcutaneous 4T1 breast carcinoma in vivo (p < 0.01) and prolonged survival of the treated 4T1 breast carcinoma mice. Moreover, the pathological damage to the cardiac tissue in mice treated with DOX-Cur NPs was significantly less severe than that of mice treated with free DOX. This study suggested that DOX-Cur NPs may have promising applications in breast carcinoma therapy.
Subject(s)
Breast Neoplasms/drug therapy , Curcumin/therapeutic use , Doxorubicin/therapeutic use , Heparin/chemistry , Nanoparticles/chemistry , Polyesters/chemistry , Polyethyleneimine/chemistry , Animals , Breast Neoplasms/pathology , Cations , Cell Death/drug effects , Cell Line, Tumor , Curcumin/adverse effects , Curcumin/pharmacology , Disease Models, Animal , Doxorubicin/adverse effects , Doxorubicin/pharmacology , Drug Carriers/chemistry , Drug Delivery Systems , Endocytosis/drug effects , Female , Flow Cytometry , Fluorescence , Heparin/chemical synthesis , Humans , Magnetic Resonance Spectroscopy , Mice , Mice, Inbred BALB C , Myocardium/pathology , Nanoparticles/ultrastructure , Polyesters/chemical synthesis , Polyethyleneimine/chemical synthesis , Tissue Distribution/drug effectsABSTRACT
BACKGROUND: Green synthesis of metallic nanoparticles (NPs) has been extensively carried out by using plant extracts (PEs) which have property of stabilizers/emulsifiers. To our knowledge, there is no comprehensive study on applying a green approach using PEs for fabrication of biodegradable PLA NPs. Conventional methods rely on molecules like polyvinyl alcohol, polyethylene glycol, D-alpha-tocopheryl poly(ethylene glycol 1000) succinate as stabilizers/emulsifiers for the synthesis of such biodegradable NPs which are known to be toxic. So, there is urgent need to look for stabilizers which are biogenic and non-toxic. The present study investigated use of PEs as stabilizers/emulsifiers for the fabrication of stable PLA NPs. Synthesized PLA NPs through this green process were explored for controlled release of the well known antioxidant molecule quercetin. METHODOLOGY/PRINCIPAL FINDINGS: Stable PLA NPs were synthesized using leaf extracts of medicinally important plants like Syzygium cumini (1), Bauhinia variegata (2), Cedrus deodara (3), Lonicera japonica (4) and Eleaocarpus sphaericus (5). Small and uniformly distributed NPs in the size range 70±30 nm to 143±36 nm were formed with these PEs. To explore such NPs for drugs/ small molecules delivery, we have successfully encapsulated quercetin a lipophilic molecule on a most uniformly distributed PLA-4 NPs synthesized using Lonicera japonica leaf extract. Quercetin loaded PLA-4 NPs were observed for slow and sustained release of quercetin molecule. CONCLUSIONS: This green approach based on PEs mediated synthesis of stable PLA NPs pave the way for encapsulating drug/small molecules, nutraceuticals and other bioactive ingredients for safer cellular uptake, biodistribution and targeted delivery. Hence, such PEs synthesized PLA NPs would be useful to enhance the therapeutic efficacy of encapsulated small molecules/drugs. Furthermore, different types of plants can be explored for the synthesis of PLA as well as other polymeric NPs of smaller size.
Subject(s)
Chemistry Techniques, Synthetic/methods , Drug Carriers/chemical synthesis , Green Chemistry Technology/methods , Nanoparticles , Plant Extracts/chemistry , Polyesters/chemical synthesis , Quercetin , Antioxidants/chemistry , Antioxidants/metabolism , Capsules , Delayed-Action Preparations , Drug Carriers/chemistry , Emulsifying Agents/chemistry , Permeability , Plants, Medicinal/chemistry , Polyesters/chemistry , Quercetin/chemistry , Quercetin/metabolism , SolubilityABSTRACT
In this study, we designed a new biocomposite comprising electrospun polycaprolactone (PCL)/fucoidan, in which the fucoidan has various beneficial biological functions, including anticoagulant, antiviral, and immunomodulatory activity. To obtain the composite scaffolds, a mixture of PCL and fucoidan was electrospun using various compositions (1, 2, 3, and 10 wt.%) of fucoidan powders. The resultant electrospun composites exhibited improved tensile modulus and strength for limited weight fractions (<10 wt.%) of fucoidan when compared with the pure PCL fiber mats. In addition, the biocomposites showed dramatic hydrophilic properties at >3 wt.% of fucoidan in the PCL/fucoidan. The biocompatibility of the electrospun mats was examined in vitro using osteoblast-like cells (MG63). Total protein content, alkaline phosphatase activity, and calcium mineralization were assessed. Scanning electron microscopic images showed that the cells were distributed more widely and were agglomerated on PCL/fucoidan mats compared with pure PCL mats. In addition, total protein content, alkaline phosphatase activity, and calcium mineralization were higher with PCL/fucoidan mats than with pure PCL mats. These observations suggest that fucoidan-supplemented biocomposites would make excellent materials for tissue-engineering applications.
Subject(s)
Biocompatible Materials/chemical synthesis , Electrochemical Techniques/methods , Polyesters/chemical synthesis , Polysaccharides/chemical synthesis , Tissue Engineering/methods , Undaria , Biocompatible Materials/pharmacology , Cells, Cultured , Humans , Osteoblasts/drug effects , Osteoblasts/physiology , Polyesters/pharmacology , Polysaccharides/pharmacologyABSTRACT
Betulin, an abundant triterpene, can be extracted from birch bark and can be used as a renewable monomer in the synthesis of microporous polyesters. Cross-linked networks and hyperbranched polymers are accessible by an A(2) + B(3) reaction, with betulin being the A(2) monomer and B(3) being a trifunctional acid chloride. Reaction of betulin with a diacid dichloride results in linear, soluble polyesters. The present communication proves that the polyreaction follows the classic schemes of polycondensation reactions. The resulting polymers are analyzed with regard to their micro-porosity by gas sorption, NMR spectroscopy, and X-ray scattering methods. The polymers feature intrinsic microporosity, having ultrasmall pores, which makes them candidates for gas separation membranes, e.g., for the separation of CO(2) from N(2) .
Subject(s)
Betula/chemistry , Gases/chemistry , Plant Extracts/chemistry , Polyesters/chemistry , Triterpenes/chemistry , Adsorption , Plant Bark/chemistry , Plant Extracts/isolation & purification , Polyesters/chemical synthesis , Porosity , Triterpenes/isolation & purificationABSTRACT
The size and surface property of nanomaterial-based delivery systems administered intravenously play important roles in their cell uptake and in vivo distribution. Both of them should be capable of self-evolution in order to achieve efficient targeting performance. A facile strategy was proposed to manipulate both the size and surface property of polymeric micelles. It was found that the hierarchical assembly between trimethylated chitosan-g-poly(ε-caprolactone) (TMC-PCL) micelles and carboxyethyl chitosan-g-poly(ethylene glycol) (CEC-PEG) could produce onion-like micelles with enlarged size and PEGylated surface. The onion-like micelles could withstand the ionic strength of plasma and competitive exchange with BSA, and abruptly disassemble into the pristine TMC-PCL micelles via a small change in pH. By varying the degree of carboxyethylation, the disassembly pH could be modulated to the range of the tumoral microclimate pH. In contrast with TMC-PCL micelles, which displayed high cytotoxicity and endocytic ability towards C6 glioma cells, the onion-like micelles were cell-friendly and internalized by the cells at a very low level. Doxorubicin was used as a model chemotherapeutic agent and incorporated within TMC-PCL micelles. Dox release from both TMC-PCL micelles and the onion-like micelles was very slow under normal physiological conditions and displayed excellent pH sensitivity. Cell viability of Dox-loaded micelles was also investigated.
Subject(s)
Micelles , Onions/chemistry , Alkylation/drug effects , Animals , Cell Death/drug effects , Cell Line, Tumor , Cell Survival/drug effects , Chitosan/chemical synthesis , Chitosan/chemistry , Doxorubicin/pharmacology , Endocytosis/drug effects , Hydrogen-Ion Concentration/drug effects , Microscopy, Confocal , Microscopy, Electron, Transmission , Osmolar Concentration , Polyesters/chemical synthesis , Polyesters/chemistry , Polyethylene Glycols/chemical synthesis , Polyethylene Glycols/chemistry , RatsABSTRACT
Novel highly functional biobased epoxy compounds, epoxidized sucrose esters of fatty acids (ESEFAs), were cross-linked with a liquid cycloaliphatic anhydride to prepare polyester thermosets. The degree of cure or conversion was studied using differential scanning calorimetry (DSC), and the sol content of the thermosets was determined using solvent extraction. The mechanical properties were studied using tensile testing to determine Young's modulus, tensile stress, and elongation at break. Dynamic mechanical analysis (DMA) was used to determine glass-transition temperature, storage modulus, and cross-link density. The nanomechanical properties of the surfaces were studied using nanoindentation to determine reduced modulus and indentation hardness. The properties of coatings on steel substrates were studied to determine coating hardness, adhesion, solvent resistance, and mechanical durability. Compared with the control, epoxidized soybean oil, the anhydride-cured ESEFAs have high modulus and are hard and ductile, high-performance thermoset materials while maintaining a high biobased content (71-77% in theory). The exceptional performance of the ESEFAs is attributed to the unique structure of these macromolecules: well-defined compact structures with high epoxide functionality. These biobased thermosets have potential uses in applications such as composites, adhesives, and coatings.
Subject(s)
Coated Materials, Biocompatible/chemical synthesis , Epoxy Compounds/chemical synthesis , Fatty Acids/chemistry , Polyesters/chemical synthesis , Sucrose/chemistry , Anhydrides/chemistry , Calorimetry, Differential Scanning , Cross-Linking Reagents/chemistry , Elastic Modulus , Elasticity , Hardness , Materials Testing , Mechanics , Solvents , Soybean Oil/chemistry , Spectroscopy, Fourier Transform Infrared , Steel , Surface Properties , Temperature , Tensile StrengthABSTRACT
In this study, the grafting of nicotinic acid and p-aminobenzoic acid (PABA) onto poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) was performed by Huisgen's 1,3-dipolar cycloaddition, also known as click chemistry. Concentrations used for grafting were 0.10, 0.20, and 0.30 molar ratios with respect to caproyl units. The grafted copolymers were successfully obtained at all ratios as confirmed by NMR, GPC, and FT-IR. According to the DSC results, the polymorphisms of these grafted copolymers were mostly changed from semicrystalline to amorphous depending on the type and the amount of grafting compounds. TGA thermograms showed different thermal stabilities of the grafted copolymers compared to the original copolymers. Cytotoxicity results from HUVEC models suggested that the toxicity of grafted nanoparticles increased with the molar ratios of grafting units. Due to differences in molecular structure between nicotinic acid and PABA, physicochemical properties (particle size and surface charge) of grafted copolymer nanoparticles were substantially different. With increasing molar ratio of the grafting units, the particle size of blank nanoparticles tended to increase, resulting from an increase in the hydrophobic fragments of the grafted copolymer. Ibuprofen was chosen as a model drug to evaluate the interaction between grafted copolymers and loaded drug. After ibuprofen loading, the particle size of the loaded nanoparticles of both grafted copolymers increased compared to that of the blank nanoparticles. Significant differences in loading capacity between nicotinic acid and PABA grafted copolymer nanoparticles were clearly shown. This is most likely a result of different compatibility between each grafting compound and ibuprofen, including hydrogen bond interaction, π-π stacking interaction, and steric hindrance.