Attenuation of cannabis withdrawal symptoms by Prosopis farcta extract, its luteolin and melatonin in mice: Involvement of brain-derived neurotrophic factor and dopamine.

The aim of this study was the identification of luteolin in Prosopis farcta extract (PFE) and melatonin to evaluate its effect on THC withdrawal syndrome in mice. Luteolin was identified by high-performance liquid chromatography (HPCL). Signs of toxicity of mice in PFE and luteolin were monitored for LD50 calculation. The behavioral symptoms of THC withdrawal (stereotypies, ambulation, and inactivity time) induced by the rimonabant challenge were illustrated in THC-dependent mice receiving PFE, luteolin, and melatonin. The expression of mature BDNF (mBDNF) was evaluated by Western blot analysis. The dopamine concentrations were measured using HPLC. PFE and luteolin LD50 were 650 and 220 mg/kg, respectively. PFE (300 mg/kg), all doses of luteolin, and melatonin increased significantly the mBDNF expression and decreased the dopamine concentration. The findings suggest that PFE, luteolin, and melatonin are mighty in reducing the signs of THC withdrawal. It seems these effects were due to a decrease in dopamine concentration level and an increase in mBDNF protein expression in mice brains.

Evaluation of the toxicological effects of Neltuma alpataco (Prosopis alpataco) pod alkaloid extract.

The pods of Neltuma spp. have shown potential as a source of protein and energy in livestock. However, prolonged consumption of some of these species can lead to neurological symptoms in ruminants. This study aimed to determine the alkaloid content, as well as the in vitro and in vivo effects of an alkaloid-enriched extract (AEE) from N. alpataco pods. High performance liquid chromatography-high resolution mass spectrometry (HPLC-HRMS) identified juliprosine and juliprosopine as primary alkaloids, with juliprosine being most abundant. AEE from N. alpataco demonstrated dose-dependent cytotoxicity on glioma cells after 48 h, with a 50% cytotoxic concentration (CC50) of 24.69 µg/mL. However, the release of LDH was observed only at the highest tested concentration, indicating cellular damage. Further examination through phase-contrast microscopy and dual acridine orange/ethidium bromide fluorescence staining revealed morphological changes consistent with an apoptotic mechanism of cell death, ultimately leading to secondary necrosis. Finally, the LD50 after intraperitoneal injection in mice was determined to be 12.98 mg/kg. Taken together, these findings demonstrated for the first time the in vivo and in vitro toxicity of the AEE from N. alpataco pods.

Application of the SeDeM system for the preparation of antiparasitic tablets from mesquite flour for use in sheep.

The use of herbal medicine to treat various diseases is becoming increasingly important as an alternative therapy. Numerous plants have been traditionally used for different purposes, including antiparasitic in humans and animals. Diseases caused by gastrointestinal parasites in ruminants, especially by the nematode Haemonchus contortus, cause large economic losses to the producers, whether by complications of the diseases or the cost of treatment. The main way of handling nematodiasis is by administering anthelmintic drugs, but their excessive use has the disadvantage of causing drug resistance; therefore, an alternative is the use of herbal medicine for this purpose. Mesquite (Prosopis spp.) has been used in Mexico to treat gastrointestinal diseases attributed to helminths. The present study aimed to characterize the rheological properties of mesquite flour using the SeDeM Expert System to determine its suitability for tablet production by direct compression. Direct compression technology facilitates the tableting process by reducing manufacturing costs. The results of the present study indicate that mesquite flour can be processed by direct compression. The latter could allow the manufacturing of economic tablets to treat infections by H. contortus in ruminants.

Prosopis juliflora (Sw.) DC phytochemicals induce apoptosis and inhibit cell proliferation signaling pathways, EMT, migration, invasion, angiogenesis and stem cell markers in melanoma cell lines.

ETHNOPHARMACOLOGICAL RELEVANCE: Prosopis juliflora (Sw.), DC is a xerophytic plant species that extensively grow in Asia, Africa, Australia, and Brazil. From ancient time P. juliflora is being utilized in various folk remedies for example in wound healing, fever, inflammation, measles, excrescences, diarrhea and dysentery. Traditionally, gum, paste, and smoke obtained from the leaves and pods are applied for anticancer, antidiabetic, anti-inflammatory, and antimicrobial purposes. AIM OF THE STUDY: Our previous studies have demonstrated the promising potential of Prosopis Juliflora leaves methanol extract (PJLME) against breast cancer, and suggested its possible integration as a complementary medicine for the effective management of breast cancer. However, evidence against how PJLME mechanistically target the cancer proliferative pathways and other targets is poorly understood. The basic aim of the present study was to understand the anti-melanoma potential of PJLME against B16f10 cells with possible mechanisms of action. MATERIALS AND METHODS: MTT assay was used to determine cell viability. Wound and transwell migration assay was performed to check migration potential of cells after PJLME treatment, while clonogenic assay was carried out to understand its colony inhibition actvity. Flow cytometry was used to perform annexin V/PI assay (apoptosis assay), ROS assay, cell cycle analysis. In-vitro angiogenesis assay was performed to check formation of capillary like vascular structure after PJLME treatment. Apoptotic genes, signaling pathways markers, EMT markers and stem cell markers were determined by western blotting. In-vivo BALB/C mice xenograft model study was performed to check the effect of PJLME on in-vivo melanoma tumor growth. RESULTS: The experimental outcome of the present study has clearly demonstrated the inhibition of growth, migration, invasion, colony formation and apoptosis inducing potential of PJLME against mouse melanoma cancer cells. Treatment of B16F10 melanoma cells with PJLME resulted in arrest of cell cycle at G0/G1 phase. Annexin V-FITC/PI assay confirmed the apoptosis inducing potential of PJLME in B16F10 and A375 melanoma cells. Furthermore, Western blot experiments confirmed that the treatment of PJLME downregulates the expression of anti-apoptotic gene like Bcl2 and increase the expression profile of pro-apoptotic genes like Bax, Bad, and Bak in B16F10 melanoma cells. HUVEC (Human umbilical vein endothelial cells) tube formation assay clearly demonstrated the anti-angiogenic potential of PJLME. The study also revealed that PJLME has potential to inhibit the Akt and Erk signaling pathways which are participating in cancer cell proliferation, migration, invasion etc. The outcome of qRT-PCR and immunoblotting analysis clearly unveiled that PJLME treatment leads to downregulation of epithelial-mesenchymal transition (EMT) as well as stem cell markers. Finally, the in-vivo animal xenograft model study also revealed the anti-melanoma potential of PJLME by significantly inhibiting the B16F10 melanoma tumor growth in BALB/c mice model. The LC-ESI-MS/MS analysis of PJLME showed the presence of variety of bioactive molecules associated with anticancer effects. CONCLUSION: The outcome of the present investigation clearly demonstrated the anti-melanoma potential of PJLME against B16f10 melanoma cells. PJLME can be explored as an adjuvant or complementary therapy against melanoma cancer, however further studies are required to understand the clinical efficacy of PJLME. Nevertheless, it can be further explored as a promising resource for identification of novel anticancer candidate drug.

Characterization of phyto-components with antimicrobial traits in supercritical carbon dioxide and soxhlet Prosopis juliflora leaves extract using GC-MS.

This study aimed to screen the bioactive compounds from Prosopis juliflora leaf supercritical fluid extract and to assess its antimicrobial properties. Supercritical carbon dioxide and Soxhlet methods were used for extraction. The extract was subjected to Gas Chromatography-Mass Spectrometer (GC-MS) and Fourier Transform Infrared for the characterization of the phyto-components. When compared to soxhlet extraction, more components (35) were eluted by supercritical fluid extraction (SFE), according to GC-MS screening. Rhizoctonia bataticola, Alternaria alternata, and Colletotrichum gloeosporioides were all successfully inhibited by P. juliflora leaf SFE extract, which demonstrated strong antifungal properties with mycelium percent inhibition of 94.07%, 93.15%, and 92.43%, respectively, compared to extract from Soxhlet, which registered 55.31%, 75.63% and 45.13% mycelium inhibition respectively. Also, SFE P. juliflora extracts registered higher zone of inhibition 13.90 mm, 14.47 mm and 14.53 mm against all three test food-borne bacterial pathogens viz Escherichia coli, Salmonella enterica and Staphylococcus aureus respectively. Results obtained from GC-MS screening revealed that SFE is more efficient than soxhlet extraction in recovering the phyto-components. P. juliflora may provide antimicrobial agents, a novel natural inhibitory metabolite.

Cytotoxic effect and induction of apoptosis in human cervical cancer cells by a wood extract from Prosopis laevigata.

Cervical cancer ranks fourth in incidence among women worldwide. Cisplatin is currently the first-line drug of treatment for cervical cancer; however, it causes serious adverse effects. Therefore, it is crucial to explore natural products for cervical cancer treatment. Prosopis laevigata is a medicinal plant frequently used for ophthalmological and gastrointestinal infections. In this study, we used the MTT cell viability assay to evaluate the cytotoxic effect of a wood extract from Prosopis laevigata (Extract T7) in SiHa, HeLa, Ca Ski, and C-33 A cancer cell lines. Phosphatidylserine translocation and cell cycle evaluations were performed to determine the mechanism of cellular death. The extract's safety was evaluated using the Ames test with Salmonella typhimurium strains, in vivo acute toxicity assay, and repeated dose toxicity assay in mice. We also identified phenolic compounds of Extract T7 through liquid chromatography/mass spectrometry. Naringin, catechin, and eriodictyol demonstrated a higher concentration in Extract T7. Additionally, Extract T7 exhibited a cytotoxic effect against cervical cancer cells, where C-33 A was the most sensitive (IC50= 22.58 ± 1.10 µg/mL and 14.26 ± 1.11 µg/mL at 24 h and 48 h respectively). Extract T7 induced death by apoptosis and cell cycle arrest in the G2 phase in C-33 A. Extract T7 was not mutagenic. No toxicological effects were observed during acute toxicity and repeated dose toxicity for 28 days. Therefore, further evaluations of Extract T7 should be conducted to identify the complete mechanism of action for potential anti-tumoral activity and safety before conducting studies in animal models.

Antimicrobial activity of the methanolic leaf extract of Prosopis laevigata.

The appearance of antimicrobial-resistant pathogens has highlighted the need to search for new compounds that can effectively combat infectious diseases. A potential source of these compounds are the secondary metabolites of species that have been reported as effective traditional treatments of such diseases. Prosopis laevigata is a medicinal plant, and its chemical constituents have shown potential antimicrobial activity. In this study, the antimicrobial activities of the methanolic extract of the leaves of Prosopis laevigata against different bacterial and fungal strains of medical and agronomic interest were investigated in vitro. In addition, the chemical composition of this extract was investigated by HPLC-DAD, GC‒MS, and HPLC‒MS. The methanolic leaf extract contained 67 mg of GAE/g of total phenols (6.7%), 2.6 mg of QE/g of flavonoids (0.26%), and 11.87 mg of AE/g of total alkaloids (1.18%). Phenolic acids and catechol were the compounds identified by HPLC-DAD. The methanolic extract had strong antimicrobial activity, especially against Staphylococcus aureus (MIC = 0.62 mg/mL), Escherichia coli (MIC = 0.62 mg/mL), Candida tropicalis (MIC = 0.08 mg/mL) and Fusarium moniliforme (MIC = 4.62 mg/mL). These results suggest that the extract of P. laevigata leaves could be a source of antimicrobial molecules. However, it is necessary to delve into its chemical composition.

New bioactive compounds characterized by liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry in hydro-methanol and petroleum ether extracts of Prosopis farcta (Banks & Sol.) J. F. Macbr weed.

The purpose of this study was to identify and characterize the chemical composition present in aerial parts of Prosopis farcta in petroleum ether and hydro-methanol extracts through LC-PDA-ESI-MS/MS and GC-MS techniques respectively for the first time. The plant samples were collected from northeast of Iran during maturity stage. LC-MS/MS profile revealed 47 phenolic compounds in hydro-methanol extracts, including phenolic acids, flavonoids, and their glycoside derivatives. Flavonoid-O-glycosides (19), flavonoid aglycones (11), phenolic acid derivatives (9), flavonoid-C-glycosides (4), and flavonoid-O, C-glycosides (1) were dominant class phenolics in all studied parts. The extracts contained a significant amount of major compounds, including gallic and vanillic acids, luteolin, apigenin, phloridzin, and vicenin-2. Also, GC-MS analysis of petroleum ether extracts showed that fatty acids, organic acids, steroids, terpenoids, and hydrocarbons were the group of major compounds in all parts. Twenty-four, 27, and 25 components were identified, which represent 99.2%, 96.1%, and 99.4% of the total composition in fruits, leaves, and stems, respectively. These results suggested that other genetic resources of P. farcta can be further explored to screen genotypes with high bioactive compounds and purification of phytochemical compounds, which are valuable to produce, expand, and develop natural antioxidants in production of bio-medicine and food.

Reproductive disruption in adult female and male rats prenatally exposed to mesquite pod extract or daidzein.

Phytoestrogens are considered to be endocrine disruptors, since they can alter the endocrine system, thus disturbing many reproductive events. The intake of diets containing a high content of phytoestrogens has increased worldwide in human populations and in domestic animals. Phytoestrogens in maternal blood can pass through the placenta to the fetus in high amounts and can have long-term organizational effects. Mesquite (Prosopis sp) is a leguminous plant widely used to feed several livestock species, and is also used in the human diet. In this study we assessed the effects of exposure to mesquite pod extract during the periconception and pregnancy periods on the reproduction of male and female descendants. The females of three experimental groups received one of the following treatments: 1) vehicle injection; 2) mesquite pod extract or 3) the isoflavone daidzein during the periconception and pregnancy periods. Estrous cyclicity, sexual behavior and hormones, as well as uterine and vaginal epithelia were evaluated in the female descendants. In the males, sexual behavior and hormones, apoptosis in testicular cells and sperm quality were evaluated. In females the following was observed: alterations in estrous cycles, decreased sexual behavior, estradiol and progesterone levels, increased uterine and vaginal epithelia. In males, we observed a decrease in sexual behavior, testosterone and sperm quality, and apoptosis increased in testicular cells. All these effects were similar to those caused by daidzein. These results indicate that prenatal exposure to mesquite pod extract or daidzein, administered to females before and during pregnancy, can disrupt normal organizational-activational programming of reproductive physiology in female and male descendants.

Genetic diversity of Prosopis juliflora in the state of Qatar and its valuable use against postharvest pathogen of mango fruits.

Mango (Mangifera indica) is the second most internationally traded tropical fruit in the world. The fruit has high nutritional value. Its susceptibility to postharvest diseases and chill injuries increases its storage cost and put stress on exploring natural products that can increase its shelf-life. Our team has previously described Prosopis juliflora water-soluble leaf ethanolic (PJ-WS-LE) extract with fungicidal effectiveness against spoiling fungi. The present study explores P. juliflora genetic diversity in the state of Qatar and the antifungal effectiveness of the leaf extract of plants collected from different locations. The study also evaluates PJ-WS-LE extract efficacy against Alternaria. alternata and Colletotrichum gloeosporioides inoculated in mango samples and the power of the extract as coating material. P. juliflora samples collected from six different locations showed genetic and antimicrobial effectiveness similarities. They showed also similarity to the sequence representing P. juliflora 18S ribosomal RNA partial sequence, accession number JX139107.1 originated from India. PJ-WS-LE extract (8 mg/ml) has 80% efficacy in controlling A. alternata in mango and it lowers C. gloeosporioides disease severity by 53.4%. PJ-WS-LE extract (8 mg/ml) embedded in 1% chitosan maintained mango quality for 5 weeks. In vivo results of PJ-WS-LE extract highlights the potentials of the extract as chemical fungicides replacement.

Prosopis juliflora piperidine alkaloid extract levels in diet for sheep change energy and nitrogen metabolism and affect enteric methane yield.

BACKGROUND: Ionophore antibiotics improve the efficiency of energy metabolism, which has driven their use as a feed additive in ruminants for decades. Currently, they have not been approved in many countries, generating a challenge for the immediate search for plant extracts with a similar mode of action on rumen metabolism. This study evaluated the effects of enriched Prosopis juliflora (mesquite) piperidine alkaloid extract (MPA) levels as an alternative phytoadditive to sodium monensin (MON) in sheep. RESULTS: The MPA diet did not differ from MON with regard to nutrient intake. A quadratic effect (P < 0.05) was observed for organic matter and neutral detergent fibre digestibility, with respective maximum point at 25.40 and minimum point at 0.95 mg kg-1 MPA. The MPA levels linearly decreased (P < 0.05) faecal nitrogen loss. MPA did not differ from MON with regard to nutrient digestibility, and MPA levels increased (P < 0.05) the proportion of digestible energy and metabolizability from dietary gross energy. The MPA levels linearly decreased (P < 0.05) enteric CH4 production, the yield showing lower (P < 0.05) energy loss as CH4 than MON. CONCLUSION: The results show that MPA levels of 17.3 and 27.8 mg kg-1 are enteric CH4 inhibitors and enhance energy and protein utilization, indicating a promising alternative to MON for ruminants. © 2022 Society of Chemical Industry.

Effect of Prosopis ruscifolia on incretin and insulin secretion in alloxan-induced hyperglycemic rats / Efecto de Prosopis ruscifolia sobre la secreción de incretina e insulina de ratas hiperglicémicas por aloxano

Background: Diabetes mellitus treatment is based on oral hypoglycemic agents or insulin. Medicinal plants constitute an option, and the leaves of Prosopis ruscifolia (Pr) were shown to be effective in reducing glycemia in hyperglycemic animals. Objective: In this paper, we report the effect of P. rusciofolia (Pr) on insulin and incretin secretion in alloxan-induced hyperglycemic rats. Methodology: The effective dose was selected, and four groups (n=10) of Wistar rats were used. Two groups with normal glycemia received water or Pr (75 mg/Kg, per os, p.o.), and two groups with hyperglycemia induced by alloxan (intraperitoneal, ip), received water or Pr (75 mg/Kg, p.o.) for 2 weeks. Oral glucose tolerance test, and incretin and insulin levels were measured at the end of the experimental period. Results: The results showed that extract promotes better tolerance to oral glucose overload, in addition to a statistically significant (p<0.001) increase in blood levels of incretin and insulin, compared to the hyperglycemic rats. Conclusion: It is concluded that the ethanolic extract of P. ruscifolialeaves has a hypoglycemic effect in hyperglycemic animals by a mechanism that involves the incretin-insulin system

Antecedentes: la diabetes mellitus es una enfermedad metabólica cuyo tratamiento se basa en el uso de agentes hipoglicemiantes orales o insulina. Una opción al tratamiento son las plantas medicinales y en ese sentido, estudios previos en animales con hojas de Prosopis ruscifolia (Pr) han demostrado efecto hipoglicemiante. Objetivo: en este trabajo se reporta el efecto de P. rusciofolia (Pr) en la secreción de insulina e incretina, en ratas hiperglicémicas por aloxano. Metodología: se emplearon cuatro grupos de ratas Wistar (n=10). Dos grupos con glicemia normal que fueron tratadas con agua Pr (75 mg/Kg, per os, p.o.) y dos grupos con hiperglicemia inducida por la inyección intraperitoneal de aloxano recibieron agua Pr (75 mg/Kg, per os, p.o.) durante dos semanas. Se midieron la tolerancia oral a la glucosa, y los niveles de incretina e insulina al final del periodo de experimentación. Resultados: se encontró que el extracto promueve una mayor tolerancia a la sobrecarga de glucosa, y además un incremento significativo (p<0.001) de los niveles de incretina e insulina en sangre, comparados al grupo de ratas hiperglicémicas. Conclusión: se concluye que e l estracto etanólico de las hojas de P. ruscifolia tienen efecto hipoglicemiante en animales hiperglicémicos por un mecanismo que incluye al sistema incretina-insulina

Consumption of the Syrian mesquite plant (Prosopis farcta) fruit and seed lyophilized extracts may have both protective and toxic effects in STZ-induced diabetic rats.

The aim of study was to investigate the antidiabetic and antioxidant properties of extracts obtained from dried Prosopis farcta fruit and seeds against streptozotocin-induced diabetes in rats. According to the results, glucose, haemoglobin A1c, α-glycosidase activity, liver and kidney damage biomarkers, and malondialdehyde contents of all of the diabetic groups were found to have increased significantly according to the control group. Furthermore, the insulin and C-peptide secretions increased, and liver malondialdehyde level decreased, which were determined as the result of fluctuations in the antioxidant enzyme activities with a dose of 400 mg/kg fruit extract, while seed extract dosages of 100 and 400 mg/kg caused an increase in hepatic demage biomarkers. It was concluded that fruit extract may have insulin secretion stimulating and lipid peroxidation inhibitory effects, whereas seed extract might have caused hepatocyte damage changes to the transport functions and membrane permeability of these cells, thus causing enzymes to leak.

Attenuating effect of Prosopis cineraria against paraquat-induced toxicity in prepubertal mice, Mus musculus.

Several herbicides, especially paraquat, are persistent organic pollutants which cause damage to humans and animals through reactive oxygen and nitrogen species. Prosopis cineraria (L.) Druce exhibits antioxidant activity and can effectively manage tremors. Therefore, the present research assessed the preventive effect of Prosopis cineraria (L.) Druce ethanolic extract (PCDE) against paraquat-induced toxicity in prepubertal mice. The plant extract was chemically characterized by a high-performance liquid chromatography-diode array detector (HPLC-DAD). The PCDE was orally administered to prepubertal mice for continuous 21 days, 2 h before paraquat exposure (2 mg/kg for consecutive 3 days per week for 3 weeks). The changes in behavior, motor coordination, memory, muscle movement, anxiety, and neurotransmitter levels in the brain were assessed. Histopathology and estimation of oxidative stress parameters in the brain, liver, kidney, and heart tissues were also carried out. HPLC-DAD analysis showed a high amount of quercetin, kaempferol, and ellagic acid derivatives in the plant extract. The PCDE showed improved muscle coordination, muscle movement and memory, and reduced anxiety in prepubertal mice. Moreover, levels of dopamine and noradrenaline were increased in the brain. It successfully ameliorated the oxidative stress in different organs by increasing the level of glutathione and superoxide dismutase and by reducing malondialdehyde. The histopathological assessment showed the plant extract effectively mitigated paraquat-induced pathological lesions in the neurons, neuroglia, hepatocytes, and kidney tissues. It is concluded from the present study that the treatment with PCDE had prevented the paraquat-induced toxicity in the brain, liver, kidney, and heart through the reduction of oxidative stress possibly due to the presence of phenolic compounds and flavonoids.

Vitexin isolated from Prosopis cineraria leaves induce apoptosis in K-562 leukemia cells via inhibition of the BCR-ABL-Ras-Raf pathway.

OBJECTIVES: Leukemia is one of the severe cancer types all around the globe. Even though some chemotherapeutic drugs are available for treating leukemia, they have various side effects. As an alternative approach, herbal drugs are focused on current research to overcome leukemia. The present work was conducted to investigate the antileukemic mechanism of active phytochemical vitexin, which was isolated from ethno-medicine (Prosopis cineraria leaf) used by traditional healers of West Bengal, India. METHODS: Antiproliferative mechanisms of selected phyto-compound against K-562 cells were evaluated using cellular uptake, morphological changes, DNA fragmentation, mitochondrial membrane potential and signaling pathways analysis. KEY FINDINGS: Vitexin exhibited cytotoxicity by reducing mitochondrial membrane potential (32.40%) and causing DNA fragmentation (84.15%). The western blotting study indicated inhibition of cell survival proteins (BCR, ABL, H-RAS, N-RAS, K-RAS and RAF) and expression of apoptotic proteins (p38, BAX and caspase-9) in leukemia cells upon treatment with vitexin. CONCLUSIONS: Based on the results, presently investigated phyto-compound vitexin could be considered for developing safe and natural drugs to treat leukemia after conducting suitable preclinical and clinical trials.

Environmentally benign Prosopis juliflora extract for corrosion protection by sorption - Gravimetric, mechanistic and thermodynamic studies.

Environmentally benign plant extract compounds have gained significant attention in the corrosion prevention applications due to their biodegradability and eco-friendliness. The adsorptive corrosion control action of Prosopis juliflora plant extract was investigated using differential mass change experiments and the mechanism was validated using Tafel and Nyquist plots. The effect of green corrosion inhibitor concentration (0-800 ppm) on corrosion rate at different solution temperatures (305.15, 310.15 and 315.15 K) in 1 M HCl was studied. The corrosion inhibitor exhibited monolayer surface coverage and confirmed by Langmuir isotherm (R2 > 0.970). The negative values of Gibbs free energy (<20 kJ/mol) proved the electrostatic interaction between the inhibitor and metal surface. The enhanced energy barrier for the corrosion process was confirmed by changes in Ea in the presence of biomass-based inhibitor on the mild steel surface. Electrochemical impedance study proved that the double layer capacitance (Cdl) decreased and the resistance increased with increase in corrosion inhibitor concentration. The surface modifications on the metal were observed using scanning electroscope imaging. ATR studies were conducted for functional group identification of the corrosion inhibitor.

Response of Prosopis farcta to gradually increased soil copper and cadmium levels based on an integrated investigation.

The impact of gradually increased soil levels of copper (Cu) and cadmium (Cd) on the medicinal plant, Prosopis farcta, irrigated with metal-enriched water was determined. Plants were treated with 2.54, 5.08, 10.16, and 20.32 µg mL-1 for Cu2+ and 6.13, 12.26, 24.52, and 49 µg mL-1 for Cd2+. The rate of phytoremediation was measured by bioconcentration factor (BCF) and the relative bioconcentration factor (RBCF). The movement of metal ions from roots to shoots was calculated as the Translocation Factor (TF). The exposure of plants to Cd or Cu decreased plant growth and increased Cd and Cu concentration in their shoots and roots. The weight of both shoots and roots decreased linearly with the increase of Cu and Cd contents in roots and shoots. Cd was more toxic than Cu as expected. The water content of shoots and roots decreased linearly as heavy metal levels increased. Prosopis farcta can take up Cu and Cd in both Cu- and Cd-contaminated soils but was more capable for transporting Cd from roots to shoots rather than Cu although more Cu is taken up by roots. Prosopis farcta is a natural accumulator of Cu and Cd and can be used in phytoremediation.CONCISE NOVEL ASPECTS OF THIS STUDYThis is the first report to show that the medicinal plant Prosopis farcta is an accumulator for Cu and Cd.This was determined by gradual addition of the metals to the soil via irrigation by heavy metal-polluted water which can provide an opportunity for the plant to develop a metal-resistance mechanism.Choosing suitable plant species for heavy metal accumulation is a critical step for successful phytoremediation of heavy metal pollutants.CORE IDEASProsopis farcta is of interest as a medicinal plant.P. farcta can take up Cu and Cd in both Cu- and Cd-contaminated soils.P. farcta transports more Cd from roots to shoots but more Cu is taken up.

Unveiling Antioxidant and Antiproliferative Effects of Prosopis juliflora Leaves against Human Prostate Cancer LNCaP Cells.

Herbal medications or formulations are regularly recommended by clinicians as a potential therapeutic method for a variety of human ailments, including cancer. Although Prosopis juliflora extracts have shown promise in anticancer activity, the effects on prostate cancer and the accompanying molecular mechanisms of action are still unexplored. This research aims at the antioxidant, antiproliferative, and apoptosis-inducing properties of Prosopis juliflora methanolic leaves extract in human prostate cancer LNCaP cells. The antioxidant ability of the extract was assessed using the DPPH (2, 2-diphenyl-2-picrylhydrazyl) and two additional reducing power tests. Antitumor activity was determined using MTT cell viability tests and LDH cytotoxicity assays. The probable mechanism of apoptotic cell death was further investigated utilizing a caspase-3 activation assay and qRT-PCR mRNA expression investigations of apoptotic-related genes. The results revealed that the methanol extract of Prosopis juliflora leaves contains alkaloids, flavonoids, tannins, glycosides, and phenols, all of which have substantial antioxidant activity. In vitro anticancer tests demonstrated that extract therapy resulted in a dose-dependent reduction in cell viability of LNCaP prostate cancer cells, but normal HaCaT cells showed no cytotoxic effects. Furthermore, plant extract therapy increased caspase-3 activation and mRNA expression of apoptotic-related genes, suggesting that this could be a mechanism for cancer cell growth suppression. The significance of Prosopis juliflora as a source of new antioxidant compounds against prostate cancer was emphasized in the current study. However, more study is needed to demonstrate the efficacy of Prosopis juliflora leaves extract in the treatment of prostate cancer.

[Prosopis spp. in allergic diseases]. / Prosopis spp. en enfermedades alérgicas.

OBJECTIVES: To demonstrate cutaneous reactivity and the participation of Prosopis pollen in patients with allergic diseases who attend the Allergy and Clinical Immunology Service of the General Hospital of Mexico, in Mexico City. METHODS: A retrospective and cross-sectional study was carried out by analyzing clinical records that had a complete medical history of patients between the ages of 3 and 79 years, with diagnoses of allergic rhinitis, asthma, and rhinoconjunctivitis. RESULTS: 1285 patients were studied; the frequency of positive skin reactivity for Prosopis was 27.78 %; 82.97 % had a diagnosis of allergic rhinitis, 4.39 % had a diagnosis of asthma, and 12.64 % had a diagnosis of rhinoconjunctivitis. CONCLUSIONS: Prosopis pollen plays an important role in diseases of allergic etiology. It is important to consider the antigenic combination with other families such as Chenopodiaceae.

Objetivos: Demostrar la reactividad cutánea y la participación del polen de Prosopis en los pacientes con enfermedades alérgicas que acuden al Servicio de Alergia e Inmunología Clínica del Hospital General de México, en la Ciudad de México. Métodos: Se llevó a cabo un estudio retrospectivo y transversal de expedientes clínicos que contaran con historia clínica completa, de pacientes entre tres y 79 años de edad con diagnósticos de rinitis, asma y rinoconjuntivitis alérgica. Resultados: Se estudiaron 1285 pacientes. La frecuencia de reactividad cutánea positiva para Prosopis fue de 27.78 %; 82.97 % cursaba con diagnóstico de rinitis alérgica, 4.39 % de asma y 12.64 % de rinoconjuntivitis. Conclusión: El polen de Prosopis participa importantemente en enfermedades de etiología alérgica. Es importante considerar el cruce antigénico con otras familias como las Chenopodiaceas.

LC-ESI-QTOF-MS/MS characterization of phenolic compounds from Prosopis farcta (Banks & Sol.) J.F.Macbr. and their potential antioxidant activities.

Prosopis farcta (Banks & Sol.) J.F.Macbr. is an emerging medicinal plant containing a diverse array of phytochemicals, including protein, fat, carbohydrate, fibre, alkaloids, fatty acids, glycosides, and polyphenols, with strong antioxidant potential. However, the screening and characterization of phenolic compounds in P. farcta is limited. This study is conducted to determine the polyphenol contents and their antioxidant activity in P. farcta leaves samples via liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS/MS) and high-performance liquid chromatography-photodiode array (HPLC-PDA). Total phenolic content (TPC), total flavonoid content (TFC), and total tannins content (TTC) were determined for polyphenol estimation. The antioxidant properties were measured by total antioxidant capacity (TAC), 2,2'-Diphenyl-2-picrylhydrazyl (DPPH), Ferric Reducing Antioxidant Power (FRAP), and 2,2"²-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). LC-ESI-QTOF-MS/MS was used to identify and characterize 47 phenolic compounds, which mainly included phenolic acids (13), flavonoids (28), other polyphenols (4), lignans (1), and stilbenes (1). According to HPLC-PDA quantification, chlorogenic acid (9.78 ± 2.15 mg/g dw) was the most abundant phenolic acid, while the main flavonoids included catechin (12.73 ± 1.29 mg/g dw) and kaempferol (7.93 ± 1.47 mg/g dw). The study demonstrated the significance of P. farcta as a rich source of phenolic compounds with antioxidant capacity that can be widely used in food, beverage, feed, and pharmaceutical applications.