ABSTRACT
Source separation of human urine (yellowwater) enhances the sustainability of wastewater management and efficiency of nutrient recovery and recycling. Storage of source-separated yellowwater is recommended prior to agronomic reuse. At this point, it is of immense interest to determine the effect of storage time on quality of yellowwater. Therefore, this study focused on examining changes in some chemical properties of raw, undiluted, freshly collected, source-separated yellowwater stored for a period of 1 year under different temperature regimes: cold (4 °C), mild (10 °C) and warm (22 °C). Chemical parameters (biochemical oxygen demand (BOD(5)), N-tot, N-NO(2), N-NO(3), N-NH(4), P-tot, K, S, and pH), with the main focus on fertiliser nutrient compounds intended for agricultural utilisation, were tested. The outcomes revealed that both nitrification and denitrification processes took place in the stored yellowwater, and an increase in the pH level of up to pH greater than 9 was observed. The study found that the main macronutrients can be well preserved in yellowwater, as there were no substantial changes in the contents of these elements over a 1 year storage period at the three temperatures tested.
Subject(s)
Fertilizers/analysis , Urine/chemistry , Adult , Biological Oxygen Demand Analysis , Child , Female , Humans , Male , Nitrates/urine , Nitrites/urine , Phosphorus/urine , Potassium/urine , Quaternary Ammonium Compounds/urine , Recycling , Sulfur/urine , Temperature , Waste Disposal, Fluid/methodsABSTRACT
BACKGROUND: Homoscedasticity (constant variance over axes or among statistical factors) is an integral assumption of most statistical analyses. However, a number of empirical studies in model organisms and humans demonstrate significant differences in residual variance (that component of phenotype unexplained by known factors) or intra-individual variation among genotypes. Our work suggests that renal traits may be particularly susceptible to randomization by genetic and non-genetic factors, including endogenous variables like age and weight. METHODS: We tested associations between age, weight and intra-individual variation in urinary calcium, citrate, chloride, creatinine, potassium, magnesium, sodium, ammonium, oxalate, phosphorus, sulfate, uric acid and urea nitrogen in 9,024 male and 6,758 female kidney stone patients. Coefficients of variation (CVs) were calculated for each individual for each solute from paired 24-hour urines. Analysis of CVs was corrected for inter-measurement collection variance in creatinine and urine volume. CVs for sodium and urea nitrogen were included to correct for dietary salt and protein. RESULTS: Age was positively associated with individual CVs for calcium and negatively associated with CVs for potassium, ammonium and phosphorus (p(FDR) < 0.01). Weight was associated with CVs for creatinine, magnesium and uric acid, and negatively associated with CVs for calcium, potassium and oxalate (p(FDR) < 0.05). CONCLUSION: Intra-individual variation changes over age and weight axes for numerous urinary solutes. Changing residual variance over age and weight could cause bias in the detection or estimation of genetic or environmental effects. New methodologies may need to account for such residual unpredictability, especially in diverse collections.
Subject(s)
Body Weight , Kidney Calculi/urine , Urinalysis/methods , Urinalysis/statistics & numerical data , Adolescent , Adult , Age Factors , Aged , Aged, 80 and over , Calcium/urine , Chlorides/urine , Citrates/urine , Creatinine/urine , Female , Humans , Magnesium/urine , Male , Middle Aged , Oxalates/urine , Phosphorus/urine , Potassium/urine , Quaternary Ammonium Compounds/urine , Sodium/urine , Sulfates/urine , Uric Acid/urine , Young AdultABSTRACT
BACKGROUND AND OBJECTIVES: Type 2 diabetes is associated with excessively low urine pH, which increases the risk for uric acid nephrolithiasis. This study was conducted to assess the metabolic basis responsible for the excessive urinary acidity of individuals with type 2 diabetes. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: Nine non-stone-forming patients who had type 2 diabetes and low urine pH and 16 age- and body mass index-matched non-stone-forming volunteers without type 2 diabetes were maintained on a constant metabolic diet for 7 days, and 24-hour urine was collected on the last 2 days of the diet. RESULTS: Urine dietary markers (potassium, sulfate, phosphorus, and urea nitrogen) were not different between the two groups. Patients with type 2 diabetes exhibited a significantly lower 24-hour urine pH (5.45+/-0.27 versus 5.90+/-0.42; P<0.01) and higher net acid excretion (NAE; 57+/-12 versus 38+/-18 mEq/d; P<0.01) compared with control subjects. The proportion of NAE excreted as ammonium (NH4+/NAE) was significantly lower in patients with type 2 diabetes than in control subjects (0.70+/-0.12 versus 0.94+/-0.36; P<0.01); however, the greater NAE in patients with type 2 diabetes was not accounted for by the differences in unmeasured urinary anions. CONCLUSIONS: The overly acidic urine in patients with type 2 diabetes persists after controlling for dietary factors, body size, and age. The lower pH is due to a combination of greater NAE and lower use of ammonia buffers in patients with diabetes, which predisposes them to uric acid urolithiasis.
Subject(s)
Diabetes Mellitus, Type 2/urine , Diabetic Nephropathies/etiology , Nephrolithiasis/etiology , Adult , Age Factors , Aged , Biomarkers/urine , Body Mass Index , Buffers , Diabetes Mellitus, Type 2/complications , Diabetes Mellitus, Type 2/diet therapy , Diabetes Mellitus, Type 2/drug therapy , Diabetic Nephropathies/urine , Female , Humans , Hydrogen-Ion Concentration , Male , Middle Aged , Nephrolithiasis/urine , Phosphorus/urine , Potassium/urine , Quaternary Ammonium Compounds/urine , Sulfates/urine , Texas , Urea/urine , Uric Acid/urineABSTRACT
The effects of diet-induced acid-base disturbances were examined in 4-week, 13-week and 18-month toxicity studies, and in a 30-month carcinogenicity study. Rats were fed a natural ingredient diet (controls), supplemented with 2% or 4% KHCO(3) (base-forming diets), or with 1% or 2.1% NH(4)Cl (acid-forming diets). Additional controls were fed 3% KCl (neutral diet providing K(+) and Cl(-) in amounts equimolar to those in the 4% KHCO(3) diet and the 2.1% NH(4)Cl diet, respectively). NH(4)Cl induced the expected metabolic acidosis, as shown by decreased base excess in blood, decreased urinary pH and increased urinary net acid excretion. KHCO(3) induced the opposite effects. KCl did not affect the acid-base balance. Clinical condition and death rate were not affected. The feeding of high levels of each salt resulted in growth retardation and increased water intake and urinary volume. Plasma potassium and urinary potassium excretion were increased with KHCO(3) and KCl. Plasma chloride was increased with NH(4)Cl, but not with KCl. Urinary calcium and phosphate excretion were increased with NH(4)Cl, but there were no indications that bone minerals were involved (weight, calcium content and fat free solid of the femur were not affected). Standard haematological and clinical chemistry parameters were not affected. Kidney weights were increased with 2.1% NH(4)Cl. Hypertrophy of the adrenal zona glomerulosa occurred with KHCO(3), KCl and NH(4)Cl, due to chronic stimulation of the adrenal cortex by either K(+) or by NH(4)Cl-induced acidosis. An early onset (from week 13) of oncocytic tubules was noted in the kidneys of rats fed KHCO(3) and, after 30 months, the incidence of this lesion was much higher than the background incidence in ageing controls. No progression to oncocytomas was noted. KCl showed only slight effects on the early onset of oncocytic tubules (from 18 months). In contrast, the severity of nephrosis and the incidence of oncocytic tubules were decreased with 2.1% NH(4)Cl, suggesting a protective effect of acidosis. The feeding of KHCO(3) resulted in hyperplasia, papillomas and carcinomas of the urinary bladder. With KCl only a slight increase in proliferative urothelial lesions was noted. Apart from these (pre-)neoplastic lesions in the urinary bladder there were no treatment-related differences in tumour response among the groups. We concluded that most of the observed changes represent physiological adaptations to the feeding of acid- or base-forming salts. Remarkable effects noted with KHCO(3), and to a far lesser extent with KCl, consisted of renal oncocytic tubules and (pre-)neoplastic lesions of the urinary bladder epithelium. NH(4)Cl-induced chronic metabolic acidosis was not associated with dissolution of alkaline bone salts in rats. Finally, a protective effect of chronic acidosis on tumour development was not found.
Subject(s)
Ammonium Chloride/toxicity , Bicarbonates/toxicity , Carcinogens/toxicity , Diet , Potassium Chloride/toxicity , Potassium Compounds/toxicity , Acid-Base Equilibrium/drug effects , Animals , Bicarbonates/urine , Blood Gas Analysis , Bone and Bones/chemistry , Bone and Bones/metabolism , Calcium/metabolism , Carcinogenicity Tests , Drinking/drug effects , Eating/drug effects , Feces/chemistry , Growth/drug effects , Hydrogen-Ion Concentration , Male , Neoplasms/epidemiology , Neoplasms/pathology , Organ Size/drug effects , Phosphorus/metabolism , Quaternary Ammonium Compounds/urine , Rats , Rats, Wistar , Sex CharacteristicsABSTRACT
BACKGROUND: Human urine is known to inhibit growth, aggregation, nucleation, and cell adhesion of calcium oxalate monohydrate (COM) crystals, the main solid phase of human kidney stones. This study tests the hypothesis that low levels of inhibition are present in men with calcium oxalate stones and could therefore promote stone production. METHODS: In 17 stone-forming men and 17 normal men that were matched in age to within five years, we studied the inhibition by dialyzed urine proteins of COM growth, aggregation, and binding to cultured BSC-1 renal cells, as well as whole urine upper limits of metastability (ULM) for COM and calcium phosphate (CaP) in relationship to the corresponding supersaturation (SS). RESULTS: Compared with normals, patient urine showed reduced COM growth inhibition and reduced ULM in relationship to SS. When individual defects were considered, 15 of the 17 patients were abnormal in one or more inhibition measurements. ULM and growth inhibition defects frequently coexisted. CONCLUSIONS: Reduced COM growth and CaP and CaOx ULM values in relationship to SS are a characteristic of male stone formers. Both defects could promote stones by facilitating crystal nucleation and growth. Abnormal inhibition may be a very important cause of human nephrolithiasis.
Subject(s)
Calcium Oxalate/chemistry , Kidney Calculi/urine , Urine/chemistry , Calcium/urine , Calcium Phosphates/chemistry , Citrates/urine , Crystallization , Humans , Male , Phosphorus/urine , Potassium/urine , Quaternary Ammonium Compounds/urine , Sex Factors , Sodium/urine , SolubilityABSTRACT
BACKGROUND: The mechanism of excess urine calcium excretion in human idiopathic hypercalciuria (IH) has not been determined but may be secondary to enhanced intestinal calcium absorption, decreased renal calcium reabsorption, and/or enhanced bone demineralization. We have developed a strain of genetic hypercalciuric stone-forming (GHS) rats as an animal model of human IH. When these GHS rats are placed on a low-calcium diet (LCD), urinary calcium (UCa) excretion exceeds dietary calcium intake, suggesting that bone may contribute to the excess UCa excretion. We used the GHS rats to test the hypothesis that bone contributes to the persistent IH when they are fed an LCD by determining if alendronate (Aln), which inhibits bone resorption, would decrease UCa excretion. METHODS: GHS rats (N = 16) and the parent strain (Ctl, N = 16) were fed 13 g/day of a normal (1.2%) calcium diet (NCD) for seven days and were then switched to a LCD (0. 02%) for seven days. Ctl and GHS rats in each group were then continued on LCD for an additional seven days, with or without injection of Aln (50 micrograms/kg/24 hrs). UCa excretion was measured daily during the last five days of each seven-day period. To determine the effects of Aln on urine supersaturation, the experiment was repeated. All relevant ions were measured, and supersaturation with respect to calcium oxalate and calcium hydrogen phosphate was determined at the end of each period. RESULTS: UCa was greater in GHS than in Ctl on NCD (7.4 +/- 0.5 mg/24 hrs vs. 1.2 +/- 0.1, GHS vs. Ctl, P < 0.01) and on LCD (3.9 +/- 0.2 mg/24 hrs vs. 0. 7 +/- 0.1, GHS vs. Ctl, P < 0.01). LCD provides 2.6 mg of calcium/24 hrs, indicating that GHS rats are excreting more calcium than they are consuming. On LCD, Aln caused a significant decrease in UCa in GHS rats and brought GHS UCa well below calcium intake. Aln caused a marked decrease in calcium oxalate and calcium hydrogen phosphate supersaturation. CONCLUSION: Thus, on a LCD, there is a significant contribution of bone calcium to the increased UCa in this model of IH. Aln is effective in decreasing both UCa and supersaturation. The Aln-induced decrease in urine supersaturation should be beneficial in preventing stone formation in humans, if these results, observed in a short-term study using the hypercalciuric stone-forming rat can be confirmed in longer term human studies.
Subject(s)
Alendronate/pharmacology , Calcium/urine , Kidney Calculi/drug therapy , Kidney Calculi/urine , Animals , Bone Resorption/drug therapy , Bone Resorption/urine , Calcium Oxalate/urine , Calcium Phosphates/urine , Calcium, Dietary/administration & dosage , Creatinine/urine , Disease Models, Animal , Female , Humans , Kidney Calculi/genetics , Magnesium/urine , Male , Phosphorus/urine , Quaternary Ammonium Compounds/urine , Rats , Rats, Mutant Strains , Rats, Sprague-DawleyABSTRACT
Progression of chronic renal failure in the Han:SPRD-cy rat polycystic kidney disease is associated with renal depletion of citric acid cycle metabolites and betaine. Amelioration of this disease by a soy protein diet is associated with retention of citric acid cycle anions, despite increased excretion, and preservation of tissue levels of betaine. As we have recently found that modest dietary supplementation with flaxseed preserves renal function and reduces histologic injury in the Han:SPRD-cy rat, we undertook a high-resolution 1H NMR spectroscopic study of urine and renal tissue extracts from Han:SPRD-cy rats to explore the renal biochemical consequences of a flaxseed diet. There was no significant dietary effect upon organic anion, methylamine, or osmolyte excretion in healthy animals. There was increased citrate excretion in Han:SPRD-cy rats fed flaxseed. Urinary ammonium excretion did not differ, suggesting that the observed increase in citrate excretion was not due to an alkaline effect of diet. Tissue extract studies revealed that disease amelioration was associated with tissue retention of succinate and betaine. Amelioration of Han:SPRD-cy rat polycystic kidney disease by diet is associated with alteration in the handling of citric acid cycle metabolites. Betaine may have a metabolic role in the reduction of chronic renal injury.
Subject(s)
Anions/metabolism , Betaine/metabolism , Citrates/urine , Citric Acid Cycle/drug effects , Isoflavones , Methylamines/metabolism , Polycystic Kidney, Autosomal Dominant/diet therapy , Quaternary Ammonium Compounds/urine , Seeds , Animals , Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Anticholesteremic Agents/pharmacology , Disease Progression , Estrogens, Non-Steroidal/pharmacology , Kidney Failure, Chronic/etiology , Kidney Failure, Chronic/prevention & control , Kidney Function Tests , Linseed Oil/pharmacology , Magnetic Resonance Spectroscopy , Male , Phytoestrogens , Plant Preparations , Polycystic Kidney, Autosomal Dominant/complications , Polycystic Kidney, Autosomal Dominant/metabolism , Rats , Rats, Mutant Strains , Succinates/metabolismABSTRACT
Metabolic acidosis associated with the catabolic state mobilizes muscle nitrogen and releases it into blood as glutamine (GLN) targeted for renal consumption and base generation. Because GLN removed by the kidneys during acidosis is a major drain on the GLN available to other sites, subsequent deprivation may lead to impaired organ function. Conversely, GLN supplementation may spare endogenous supplies and restore organ function. To test this, Sprague-Dawley rats weighing between 250 and 350 g were pair-fed elemental diets supplemented with GLN 4.9 g/L (GLN-ED) or an equivalent mixture of neutral amino acids substituted for GLN (ED). Acid loading was effected by adding hydrochloric acid to the liquid diet (110 mmol/L). Animals were studied in metabolic cages for five consecutive 24-hour urine collection periods and then anesthetized for short-term studies of interorgan fluxes and tissue GLN content. Acidosis effected an increase in ammonium nitrogen excretion (fivefold) and a reciprocal decrease (24%) in urea nitrogen excretion. Enteral GLN had no effect on the acidosis-effected ammonium (2170 +/- 71 vs 2059 +/- 361 mumol/100 g, ED vs GLN-ED, respectively) or urea excretion (5522 +/- 95 vs 5915 +/- 984 mumol/100 g, ED vs GLN-ED, respectively). Although arterial blood GLN was not increased in the GLN-ED group (531 +/- 58 vs 438 +/- 51 nmol/mL, p = .10), both liver and muscle GLN were elevated (11,650 +/- 1137 nmol/g vs 7063 +/- 578 and 5503 +/- 489 and 4742 +/- 333 nmol/g, respectively, each p < .05).(ABSTRACT TRUNCATED AT 250 WORDS)
Subject(s)
Acidosis/therapy , Glutamine/metabolism , Glutamine/therapeutic use , Alanine/metabolism , Animals , Chronic Disease , Creatinine/urine , Diet , Glutamates/metabolism , Glutamic Acid , Glutamine/administration & dosage , Liver/metabolism , Male , Methylhistidines/urine , Muscles/metabolism , Quaternary Ammonium Compounds/urine , Rats , Rats, Sprague-Dawley , Urea/urineABSTRACT
To investigate the significance of the lowered plasma vitamin A during the last few weeks of pregnancy, the capacity of erythrocyte lysates to destroy vitamin A in vitro and the alteration in ammonium nitrogen to creatinine ratio (Am N/Cr) in random urine samples was followed up at different gestational ages in women from low income groups. One group received no supplementation and the other received supplements of 1800 retinol equivalents (RE) and at most points the number of observations were not less than 12. In the unsupplemented women there was a significant increase in the capacity of erythrocytes to destroy vitamin A in vitro, which reflects an increased oxidative stress as compared to the supplemented group. While differences in mean Am N/Cr did not follow any consistent pattern, there was a strong association between lowered plasma vitamin A (< 35 micrograms/dl) and increased Am N/Cr ratio (> 0.5). This was true only when combined data of both groups were considered till 26 weeks of gestation and not beyond. As expected in supplemented women there was no significant fall in the plasma vitamin A during term. These data reveal that the lowered plasma vitamin A levels are suggestive of greater risk of hypovitaminosis A during pregnancy as indicated by the oxidative stress in erythrocytes.
Subject(s)
Creatinine/urine , Erythrocytes/metabolism , Pregnancy/blood , Quaternary Ammonium Compounds/urine , Vitamin A/blood , Chi-Square Distribution , Colorimetry , Diterpenes , Female , Ferrous Compounds/administration & dosage , Ferrous Compounds/therapeutic use , Follow-Up Studies , Hemolysis , Humans , Retinyl Esters , Sensitivity and Specificity , Time Factors , Vitamin A/administration & dosage , Vitamin A/analogs & derivatives , Vitamin A/metabolism , Vitamin A/pharmacology , Vitamin A/therapeutic useABSTRACT
A biokinetic model was used to simulate retention and excretion of two forms of U: ammonium diuranate (ADU), a relatively soluble form, and U3O8, a relatively insoluble form. These two U forms represent those most likely to be encountered in the U milling industry. The simulation model was compared with results from a study of aerosols of commercial refined U ore inhaled by laboratory animals. Beagle dogs were exposed by inhalation to ADU aerosols to achieve a median initial body burden of 0.058 mg U kg-1 body weight (within a range of 0.016 to 0.64 mg U kg-1), or to U3O8 aerosols to achieve a median retained body burden of 0.28 mg U kg-1 (0.030-0.81 mg U kg-1). The simulation model accurately described the accumulation of nephrotoxic concentrations of U in kidneys of animals exposed to ADU. Very small fractions of the initial body burden of U3O8 were translocated to kidney, and these fractions were overestimated by the model. The model showed general agreement with results of other laboratory animal studies and with available information from human exposures to ADU, UF6, or U3O8.
Subject(s)
Models, Biological , Uranium Compounds , Uranium/urine , Administration, Inhalation , Aerosols , Animals , Computer Simulation , Dogs , Female , Humans , Male , Oxides/administration & dosage , Oxides/urine , Quaternary Ammonium Compounds/administration & dosage , Quaternary Ammonium Compounds/urine , Species Specificity , Tissue Distribution , Uranium/administration & dosageABSTRACT
A Japanese boy with Lesch-Nyhan syndrome who passed xanthine calculi is reported. After pyelolithotomy for a left renal stone, made up of ammonium urate, associated with urinary tract infection, a high dose of allopurinol was given because of the persistence of pyuria. In the present case, the administration of a high dose of allopurinol, given for the prevention of ammonium urate stone formation in infected urine, induced xanthine calculi formation and we had difficulty in the management of this patient with Lesch-Nyhan syndrome associated with urinary tract infection. However, we believe it a basic necessity to cure our patient of his urinary tract infection and prevent recurrent ammonium urate stone formation because of the risk of renal deterioration.
Subject(s)
Kidney Calculi/urine , Lesch-Nyhan Syndrome/urine , Urinary Tract Infections/urine , Xanthines/urine , Allopurinol/adverse effects , Allopurinol/therapeutic use , Child , Combined Modality Therapy , Enterococcus faecalis , Humans , Kidney Calculi/chemically induced , Male , Proteus Infections/urine , Quaternary Ammonium Compounds/urine , Streptococcal Infections/urine , Uric Acid/urine , XanthineABSTRACT
Six women, aged 38 to 62 yr, participated in a 40-day metabolic study to investigate the effect of level of protein intake and of sodium bicarbonate ingestion on urinary calcium, net calcium balance, net renal acid excretion, and arterialized venous blood pH and bicarbonate ion concentration. The diet contained 44 g protein during the first 16 days and 102 g during the remaining 24 days. During the last 10 days of the study, 5.85 g of sodium bicarbonate was ingested concomitantly with the higher protein intake. Calcium, phosphorus, and magnesium intakes were held constant at 500, 900, and 300 mg, respectively. The increase in protein intake significantly increased urinary calcium and net renal acid excretion and the mean net calcium balance became negative. The ingestion of sodium bicarbonate alkalinized the urine and reversed the increase in urinary calcium associated with the higher protein intake; the mean net calcium balance became positive. The arterialized venous blood pH and bicarbonate ion concentrations were not significantly affected by dietary treatments. The results suggest that the ingestion of a small amount of sodium bicarbonate may be an effective way to increase calcium retention in women with protein-induced hypercalciuria.
Subject(s)
Acid-Base Equilibrium , Bicarbonates/administration & dosage , Calcium/metabolism , Dietary Proteins/administration & dosage , Age Factors , Bicarbonates/metabolism , Calcium/urine , Hydrogen-Ion Concentration , Magnesium/metabolism , Phosphorus/metabolism , Quaternary Ammonium Compounds/urine , Sodium BicarbonateABSTRACT
A human metabolic study was conducted to observe the effect of level of protein intake on urinary calcium, calcium absorption and calcium balance in older adults and to further study the mechanisms of protein-induced hypercalciuria. An increase in protein intake from about 47 to 112 g while maintaining calcium, magnesium and phosphorus intakes constant caused an increase in urinary calcium and a decrease in calcium retention. Glomerular filtration rate was increased and fractional renal tubular reabsorption was decreased by the increase in protein intake; total renal acid, ammonium and sulfate excretions more than doubled, whereas urinary sodium decreased by 38%. The changes in urinary calcium were positively correlated with the increase in total renal acid and sulfate excretion as well as with the decrease in fractional renal tubular reabsorption of calcium. Thus, the data indicate that protein-induced hypercalciuria is due to an increase in glomerular filtration rate and a decrease in fractional renal tubular reabsorption of calcium, the latter of which may be caused by the increased acid load on the renal tubular cells.
Subject(s)
Calcium/metabolism , Dietary Proteins/pharmacology , Kidney/physiology , Adult , Aged , Calcium/urine , Calcium, Dietary , Female , Humans , Magnesium/blood , Male , Middle Aged , Phosphorus/blood , Quaternary Ammonium Compounds/urine , Sodium/urine , Sulfates/urineABSTRACT
Urinary ammonium excretion, in vitro ammoniagenesis and the activities of renal cortical phosphate-dependent glutaminase (PDG) and glutamic dehydrogenase (GLDH) were measured in rats with a reduced renal mass. Following contralateral nephrectomy, ammonium excretion per nephron, ammonia production and the activities of PDG and GLDH were all increased significantly in remnant kidneys of rats fed high protein diets. In rats fed low protein diets, although PDG activity increased, GLDH activity and ammonia production and excretion did not increase in remnant kidneys following contralateral nephrectomy. Ammonia production and excretion were greater in rats fed high than low protein diets, a difference that was corrected by the addition of mineral acid to the diets of low protein-fed rats. Acid supplementation to the low protein group did not result in enhanced ammonia production or GLDH activity following a reduction in renal mass. The data indicate that the increased rate of ammoniagenesis which occurs following nephron reduction is markedly influenced by dietary protein content. A lack of enhanced GLDH activity may underlie the lack of increased ammonia production of low protein-fed rats following nephron reduction.