Subject(s)
Biological Therapy/methods , CRISPR-Cas Systems/genetics , Citrus sinensis , Gene Editing , Plant Diseases/microbiology , Plant Diseases/prevention & control , Plant Viruses/pathogenicity , Rhizobiaceae/virology , Citrus sinensis/genetics , Citrus sinensis/microbiology , Citrus sinensis/virology , Disease Resistance/genetics , Florida , Food Industry , Food, Genetically Modified/microbiology , Food, Genetically Modified/virology , Genes, Plant/genetics , Plant Diseases/genetics , Plant Diseases/virology , Rhizobiaceae/pathogenicity , Spinacia oleracea/genetics , United StatesABSTRACT
Three lytic phages (PhiRP1, PhiRP2, and PhiRP3) specific for Robinia pseudoacacia rhizobia were isolated from the soil under black locust. They were characterized by their morphology, host range, and some other properties including DNA molecular weights. Studied phages have been found to belong to Siphoviridae family that comprises viruses with long, and noncontractile tails. They had broad host ranges and effectively lysed not only Robinia pseudoacacia microsymbionts but also different Mesorhizobium species. The phages were homogenous in latent periods (300 min) but heterogeneous in burst sizes (100-200 phage particles per one infected cell) and rise periods (90-120 min). They showed a distinct adsorption rate to Robinia pseudoacacia rhizobia (70.4-93.94%). The molecular weights of phage DNAs estimated from restriction enzyme digests were in the range from ca. 82 kb to ca. 105 kb.